Abstract:
AIM:To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS:Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study. Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR. Loss of heterozygosity (LOH) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples. Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR. RESULTS:The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%. The LOH frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively. Fifty percent LOH was found at two sites and 17% LOH at three sites. Apaf-1 mRNA expression decreased significantly in 13 cases (rs=0.487, P=0.003). The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples. Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA rs=0.886, P=10(-6)). CONCLUSION:The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Wang HL,Bai H,Li Y,Sun J,Wang XQdoi
10.3748/wjg.v13.i38.5060subject
Has Abstractpub_date
2007-10-14 00:00:00pages
5060-4issue
38eissn
1007-9327issn
2219-2840journal_volume
13pub_type
杂志文章abstract:AIM:To identify the susceptible gene (s) for type 2 diabetes in the previously mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes. METHODS:Twenty three SNPs located in 10 candidate genes i...
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pub_type: 杂志文章
doi:10.3748/wjg.v9.i9.2078
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-06-28 00:00:00
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doi:10.3748/wjg.v25.i8.941
更新日期:2019-02-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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pub_type: 杂志文章,meta分析,评审
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更新日期:2014-12-14 00:00:00
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pub_type: 杂志文章
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pub_type: 社论,评审
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2009-02-14 00:00:00
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journal_title:World journal of gastroenterology
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i5.970
更新日期:2003-05-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.4709
更新日期:2008-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.2038
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i36.5498
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i9.2121
更新日期:2003-09-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i14.20
更新日期:2005-04-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i5.915
更新日期:2003-05-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
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pub_type: 杂志文章,meta分析
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i8.1192
更新日期:2006-02-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i9.2818
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i25.3899
更新日期:2005-07-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i38.5863
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2006-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2010-12-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 评论,信件
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更新日期:2011-06-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i28.4492
更新日期:2006-07-28 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-07 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-28 00:00:00