Abstract:
:Recent investigations have demonstrated that polyphenolic catechins inhibit cancer cell proliferation and tumor growth. However, how the major active component of tea catechins, epigallocatechin-3 gallate (EGCG), mediates anticancerous effects has not been extensively examined. We have investigated the cell growth inhibitory effects of EGCG on cell growth of the human breast cancer cell line MCF-7, and the mechanism of its action with emphasis on the regulation of tumor cell survival. A significant EGCG dose-dependent growth inhibition was observed coordinated with EGCG-induced apoptosis. Analysis of survivin expression after addition of EGCG showed that both survivin mRNA and protein were decreased. The survivin-promoter luciferase activity in EGCG-treated cells was significantly inhibited by 91+/-2.0% (P<0.001), compared with the control. Interestingly, EGCG strongly inhibited the basal activation of phospho-AKT and AKT kinase activity as early as 30 min after treatment. Furthermore, inhibition of AKT kinase activity by EGCG preceded the suppression of survivin (1 h post treatment), followed by increased caspase-9 activity (6 h post treatment). A dominant negative AKT or the phosphatidylinositol 3-kinase inhibitor, LY294002, also strongly inhibited survivin promoter activity, providing further evidence to support the hypothesis that the inhibitory effect of EGCG on survivin is mediated via the AKT pathway. Therefore, EGCG is a potent proapoptotic agent in MCF-7 breast cancer cells that targets survivin expression via suppression of the AKT pathway.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Tang Y,Zhao DY,Elliott S,Zhao W,Curiel TJ,Beckman BS,Burow MEsubject
Has Abstractpub_date
2007-10-01 00:00:00pages
705-11issue
4eissn
1019-6439issn
1791-2423journal_volume
31pub_type
杂志文章abstract::Prostate cancer is the most commonly diagnosed cancer and the second leading cause of cancer related deaths in men in the United States. Ciprofloxacin is a relatively non-toxic antibiotic that can be easily administered orally with large volume of distribution and good tissue penetration. Studies from others and our l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::The potential of arsenic trioxide (As2O3) as a novel therapy against ovarian cancer has been progressively recognized. Its prospective usefulness for treatment of this malignancy either alone or in combination with other chemotherapeutic agents has been increasingly explored. In this study, we attempted to enhance the...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.986
更新日期:2011-06-01 00:00:00
abstract::Meningiomas are the most commonly occurring tumors of the central nervous system including the brain and spinal cord. Malignant meningiomas are highly aggressive and frequently recur after surgical resection of the tumor. Our previous studies have reported that urokinase plasminogen activator receptor (uPAR) and matri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::In the study of 69 matched pairs of lung tumors and normal lung parenchyma, the median activities of cathepsin B (CB) and cystein proteinase inhibitors (CPI's) in tumors were found to be increased 4.4-fold and twofold, respectively. The levels of CB and CPI activities did not correlate with TNM stages nor with cell di...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.1.77
更新日期:1994-07-01 00:00:00
abstract::Interferon (IFN) is a promising drug for prevention and treatment of hepatocellular carcinoma (HCC) in combination with chemotherapeutic agents. We previously reported that the spectra of antiproliferative activity and synergistic effect of IFN-beta when combined with anticancer drugs are more potent than those of IFN...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::MicroRNAs (miRNAs) were reported to be involved in the development of clear cell renal cell carcinoma (ccRCC). However, the study on miRNAs in ccRCC is far from complete. The present study identified miRNAs which could act as potential novel prognostic markers for ccRCC, and analyzed its possible mechanism. We found t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3746
更新日期:2016-12-01 00:00:00
abstract::The prognosis of patients carrying glottic squamous cell carcinomas (GSCCs) involving the anterior commissure is often unpredictable. In order to assess the possible prognostic role of new and reliable parameters, p53 and cyclin D1 protein expression was immunohistochemically analysed in pathological samples from 27 p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.3.553
更新日期:1996-09-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is promising for cancer treatment owing to its selective cytotoxicity against malignant cells. However, some cancer cell types, including malignant melanoma cells, are resistant to TRAIL-induced apoptosis. Therefore, dr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1483
更新日期:2012-08-01 00:00:00
abstract::Breast cancer (BC) continues to affect the lives of millions of women worldwide. Several members of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) subfamily are involved in tumor progression. Notably, the CEACAM subfamily harbors the already established cancer biomarker CEA, as well as other pote...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1860
更新日期:2013-05-01 00:00:00
abstract::One hundred five patients with clinical stage T2-T4 transitional cell bladder carcinoma who completed either low-dose (20-26 Gy) or high dose (40-50 Gy) preoperative radiotherapy (RT) followed by cystectomy were reviewed. Five-year actuarial survival was 42%, 52%, and 17% for stages T2, T3, and T4, respectively. Five-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.5.555
更新日期:1992-10-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.4.613
更新日期:1993-04-01 00:00:00
abstract::WNT signals are transduced to the planar cell polarity (PCP) pathway or the beta-catenin pathway. Drosophila Frizzled (Fz), Starry night (Stan), Van Gogh (Vang), Dishevelled (Dsh), Prickle (Pk), Diego (Dgo) and Nemo (Nmo) are implicated in the PCP signaling pathway. Choi and Benzer identified Drosophila Nmo in 1994, a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Antisense oligonucleotides that selectively inhibit gene expression are a genetic approach for disease treatment and prevention. However, their use as therapeutic agents is complicated by their low rate of transport across cellular membranes and their sequestration within endocytic-like vesicles. We report that the ad...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.4.841
更新日期:2000-10-01 00:00:00
abstract::We performed this study in order to evaluate the impact of the chemokine CXCL12 and its single-nucleotide polymorphism (SNP) rs1801157 on clinicopathological parameters and survival in patients undergoing surgery for esophagogastric cancer. The expression pattern of CXCL12 and its polymorphisms were analyzed by RT-PCR...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1044
更新日期:2011-08-01 00:00:00
abstract::Lung cancer is one of the most common types of cancer worldwide. Understanding the molecular mechanisms underlying the development and progression of lung cancer may improve early diagnosis, treatment and prognosis. The aim of the present study was to examine the pathogenesis of lung cancer and to identify potentially...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4926
更新日期:2020-01-01 00:00:00
abstract::This study examines the role of LEF1, a component of the Wnt signaling pathway, in human breast and murine mammary carcinoma and its relationship to ErbB2 (her-2/neu) expression. Mammary tissue and tumors from 5 different Wnt pathway-activated transgenic mouse strains and 5 different ErbB2 pathway-activated transgenic...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::Thyroid cancer is a common endocrine cancer, of which papillary thyroid cancer (PTC) is the most common type. Neuregulin 1 (NRG1), a glycoprotein mediating cell‑cell signaling, plays vital roles in cellular activities; however, its role in PTC progression remains poorly understood. In this study, we performed immunohi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4426
更新日期:2018-08-01 00:00:00
abstract::Bone morphogenetic protein 7 (BMP-7) is an important regulator of cell development and differentiation of various organs. Tumorigenesis and tumor progression are also strongly associated with changes of the fate of cells which are highly differentiated in healthy tissues. Therefore, we studied the role of BMP-7 in bre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Lymph node metastasis of breast cancer is a clinical marker of poor prognosis. Yet, there exist no therapies targeting mechanisms of intravasation into lymphatics. Herein we report on an effect of the antidyslipidemic drug fenofibrate with vasoprotective activity, which attenuates breast cancer intravasation in vitro,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3956
更新日期:2017-05-01 00:00:00
abstract::Postoperative adjuvant chemotherapy reportedly improves advanced colorectal cancer patients' survival, however, it is necessary to assess what regimens are useful. Doxifluridine (5'-DFUR) is an intermediate of capecitabine approved in Europe and USA to treat metastatic colorectal cancer. 5'-DFUR is metabolized to 5-fl...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-07-01 00:00:00
abstract::We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts. Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Cutaneous melanoma is an aggressive cancer with a poor prognosis for patients with advanced disease. The identification of several key molecular pathways implicated in the pathogenesis of melanoma has led to the development of novel therapies for this devastating disease. In melanoma, both the Ras/Raf/MEK/ERK (MAPK) a...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2014.2481
更新日期:2014-08-01 00:00:00
abstract::Aurora kinase A (AURKA) regulates the cell cycle checkpoint and maintains genomic integrity. AURKA is overexpressed in various malignant tumors and its upregulation induces chromosomal instability, which leads to aneuploidy and cell transformation. To investigate the role of AURKA in endometrial cancer, we evaluated t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2842
更新日期:2015-04-01 00:00:00
abstract::Sterile alpha motif and histidine/aspartic acid domain‑containing protein 1 (SAMHD1), the only deoxynucleotide triphosphate (dNTP) hydrolase in eukaryotes, plays a crucial role in regulating the dynamic balance and ratio of cellular dNTP pools. Furthermore, SAMHD1 has been reported to be involved in the pathological p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.4988
更新日期:2020-04-01 00:00:00
abstract::Recently, a novel candidate tumor suppressor gene, DPC 4/SMAD 4, has been implicated in the development of pancreatic cancers. Its location at human chromosome 18q21 prompted us to investigate this gene in a large series of primary tumors located outside the gastrointestinal tract which have been associated with loss ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::The effect of reduced glutathione (GSH) and ethanol on aflatoxin B1 (AFB1)-induced DNA single strand breaks was studied in primary cultured hepatocytes. Buthionine sulfoximine (BSO) which decreased intracellular GSH to 13% of those of the control levels increased DNA fragmentation of AFB1-treated hepatocytes by over 1...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.1.123
更新日期:1994-01-01 00:00:00
abstract::This retrospective study looks at the differences between adolescents (15-19 years) and young adults (20-25 years), diagnosed with Hodgkin lymphoma and treated at the same adult institution. Outcome according to risk category was evaluated, and although there were no significant differences in the whole cohort, or low...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.33.1.145
更新日期:2008-07-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
abstract::Specific RE protein species modulated by autocrine TGF alpha are unknown. HCT116 cells transfected with TGF alpha antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RE species. Nutrients alone (N) induced maximal mitog...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.6.1025
更新日期:1996-06-01 00:00:00
abstract::Thirty-three adult patients with anaplastic astrocytoma or glioblastoma multiforme, verified between 1990 and 1997, received postoperative intra-arterial ACNU chemotherapy and irradiation. Since our previous strategies to malignant gliomas had included intra-venous ACNU, the purpose of the present study is to evaluate...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:10.3892/ijo.13.3.537
更新日期:1998-09-01 00:00:00