Validation of a short tandem repeat multiplex typing system for genetic individualization of domestic cat samples.

Abstract:

AIM:To conduct developmental validation studies on a polymerase chain reaction (PCR) based short tandem repeat (STR) multiplex typing system, developed for the purpose of genetic individualization and parentage testing in domestic cat samples. METHODS:To evaluate reproducibility of the typing system, the multiplex was amplified using DNA extracted from hair, blood, and buccal samples obtained from the same individual (n=13). Additional studies were performed to evaluate the system's species' specificity, using 26 North American mammalian species and two prokaryotes Sacchromyces and Escherichia coli, sensitivity, and ability to identify DNA mixtures. Patterns of Mendelian inheritance and mutation rates for the 11 loci were directly examined in a large multi-generation domestic cat pedigree (n=263). RESULTS:Our studies confirm that the multiplex system was species-specific for feline DNA and amplified robustly with as little as 125 picograms of genomic template DNA, demonstrating good product balance. The multiplex generated all components of a two DNA mixture when the minor component was one tenth of the major component at a threshold of 50 relative fluorescence units. The multiplex was reproducible in multiple tissue types of the same individual. Mutation rates for the 11 STR were within the range of sex averaged rates observed for Combined DNA Index System (CODIS) loci. CONCLUSION:The cat STR multiplex typing system is a robust and reliable tool for the use of forensic DNA analysis of domestic cat samples.

journal_name

Croat Med J

journal_title

Croatian medical journal

authors

Coomber N,David VA,O'Brien SJ,Menotti-Raymond M

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

547-55

issue

4

eissn

0353-9504

issn

1332-8166

journal_volume

48

pub_type

杂志文章
  • The efficacy and adverse effects of budesonide in remission induction treatment of autoimmune hepatitis: a retrospective study.

    abstract:AIM:To compare the early biochemical response and rate of adverse effects in patients who received prednisolone (PRED)/azathioprine (AZA) and those who received budesonide (BUD)/AZA as the first-line treatment for autoimmune hepatitis. METHODS:The study involved 25 patients receiving PRED 30 mg/day + AZA 50 mg/day and...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Binicier OB,Günay S

    更新日期:2019-08-31 00:00:00

  • Breastfeeding practices in Mostar, Bosnia and Herzegovina: cross-sectional self-report study.

    abstract:AIM:To investigate breastfeeding practices among mothers of infants in the city of Mostar, Bosnia and Herzegovina. METHODS:The cross-sectional self-report study was conducted among 326 mothers of infants visiting the Advisory Center for Infants at the Medical Center in Mostar and at the Children's Department of Mostar...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Simić T,Sumanović-Glamuzina D,Boranić M,Vuksić I,Boban A

    更新日期:2004-02-01 00:00:00

  • Raman microspectroscopy as a biomarking tool for in vitro diagnosis of cancer: a feasibility study.

    abstract:AIM:To elucidate whether Raman spectroscopy aided by extensive spectral database and neural network analysis can be a fast and confident biomarking tool for the diagnosis of various types of cancer. METHODS:Study included 27 patients with 11 different malignant tumors. Using Raman microscopy (RM) a total of 540 Raman ...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2012.53.551

    authors: Pavićević A,Glumac S,Sopta J,Popović-Bijelić A,Mojović M,Bacić G

    更新日期:2012-12-01 00:00:00

  • Opinions of Zagreb University students on professor-student dating.

    abstract:AIM:To investigate what Zagreb University students think about professor-student dating. METHODS:We surveyed 500 students from ten different Zagreb University schools using anonymous questionnaires. Twenty-five female and 25 male students from each school were asked to assess professor-student dating as either accepta...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Pulanić D,Skunca M,Jureković R,Obadić A,Ostojić-Pulanić B,Petrovecki M

    更新日期:2001-02-01 00:00:00

  • Long-term cognitive deficits in patients with good outcomes after aneurysmal subarachnoid hemorrhage from anterior communicating artery.

    abstract:AIM:To evaluate long-term cognitive consequences of subarachnoid hemorrhage with good outcome and the opinion of patients and their relatives about these consequences. METHODS:The study included 10 patients surgically treated for subarachnoid hemorrhage due to the rupture of aneurysm of the anterior communicating arte...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Ravnik J,Starovasnik B,Sesok S,Pirtosek Z,Svigelj V,Bunc G,Bosnjak R

    更新日期:2006-04-01 00:00:00

  • Levels of self-reported depression and anxiety among HIV-positive patients in Albania: a cross-sectional study.

    abstract:AIM:To gain an initial perspective of mental health issues facing the Human Immunodeficiency Virus (HIV)-positive population at the University Hospital Center of Tirana (UHCT) HIV/AIDS Ambulatory Clinic. METHODS:From June-August 2009, we conducted semi-structured interviews with 79 patients (93% response rate) at the ...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2011.52.622

    authors: Morrison SD,Banushi VH,Sarnquist C,Gashi VH,Osterberg L,Maldonado Y,Harxhi A

    更新日期:2011-10-15 00:00:00

  • Plasma concentration of immunoglobulin classes and subclasses in children with autism in the Republic of Macedonia: retrospective study.

    abstract:AIM:To examine plasma concentration of IgA, IgM, IgG classes, and IgG1, IgG2, IgG3, and IgG4 subclasses in children with autism. METHODS:Infantile autism was diagnosed by the Diagnostic and Statistical Manual for Mental Disorders (DSM)-IV and the International Classification of Diseases (ICD)-10 criteria. Plasma sampl...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Trajkovski V,Ajdinski L,Spiroski M

    更新日期:2004-12-01 00:00:00

  • International pediatric cardiac assistance in Croatia: results of the 10 year program.

    abstract:AIM:In an effort to help alleviate the lack of an adequate pediatric cardiac surgical service that existed in Croatia following 1991 the International Childrens Heart Foundation (ICHF) was asked to provide the necessary surgery. Initially, this project was undertaken as a humanitarian program to provide pediatric cardi...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Novick WM,Anić D,Ivancan V,Di Sessa TG

    更新日期:2004-08-01 00:00:00

  • Thyroid function and autoimmunity in sarcoidosis: a case-control study.

    abstract:AIM:To evaluate thyroid function and the presence of antithyroid autoantibodies in patients with sarcoidosis, compared to patients with chronic obstructive pulmonary disease (controls). METHODS:Sera were obtained from 26 patients (19 women and 7 men) with active sarcoidosis, age and sex matched to 26 patients with dia...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Ilias I,Panoutsopoulos G,Batsakis C,Nikolakakou D,Filippou N,Christakopoulou I

    更新日期:1998-12-01 00:00:00

  • Comparison of preprocessing methods and storage times for touch DNA samples.

    abstract:AIM:To select appropriate preprocessing methods for different substrates by comparing the effects of four different preprocessing methods on touch DNA samples and to determine the effect of various storage times on the results of touch DNA sample analysis. METHOD:Hand touch DNA samples were used to investigate the det...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2017.58.4

    authors: Dong H,Wang J,Zhang T,Ge JY,Dong YQ,Sun QF,Liu C,Li CX

    更新日期:2017-02-28 00:00:00

  • Dental identification after two mass disasters in Croatia.

    abstract:AIM:To determine the usefulness of dental methods in the identification of victims in the railway accident in Zagreb (August 30, 1974) and midair collision of a British and a Slovenian airplane near Vrbovec (September 10, 1976). METHODS:There were 152 people killed in the railway accident, and 176 fatalities in the pl...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Dumancić J,Kaić Z,Njemirovskij V,Brkić H,Zecević D

    更新日期:2001-12-01 00:00:00

  • Assessment of psychological and social factors in adolescents risk behavior: questionnaire study.

    abstract:AIM:To investigate the influence of psychological and social factors on health risk behaviors, such as smoking, alcohol and psychoactive drug consumption among adolescents and to determine the prevalence of consuming various psychoactive substances. METHODS:The survey was conducted among adolescents in the Primorsko-g...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Malatestinić D,Mićović V,Kendel G,Baricev-Novaković Z

    更新日期:2005-02-01 00:00:00

  • Y-chromosome short tandem repeat intermediate variant alleles DYS392.2, DYS449.2, and DYS385.2 delineate new phylogenetic substructure in human Y-chromosome haplogroup tree.

    abstract:AIM:To determine the human Y-chromosome haplogroup backgrounds of intermediate-sized variant alleles displayed by short tandem repeat (STR) loci DYS392, DYS449, and DYS385, and to evaluate the potential of each intermediate variant to elucidate new phylogenetic substructure within the human Y-chromosome haplogroup tree...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2009.50.239

    authors: Myres NM,Ritchie KH,Lin AA,Hughes RH,Woodward SR,Underhill PA

    更新日期:2009-06-01 00:00:00

  • Mixed stains from sexual assault cases: autosomal or Y-chromosome short tandem repeats?

    abstract::We analyzed forensic DNA samples from four cases of sexual assault, using the Y-chromosome-specific human DNA markers and a panel of autosomal short tandem repeats (STRs). The presence of male contribution was evaluated by the analysis of the Amelogenin locus. A panel of tetrameric Y-STR (DYS19, DYS390, DYS391, DYS392...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Cerri N,Ricci U,Sani I,Verzeletti A,De Ferrari F

    更新日期:2003-06-01 00:00:00

  • Epidemiology of central nervous system tumors in Labin area, Croatia, 1974-2001.

    abstract:AIM:To establish the incidence of tumors of the central nervous system (CNS) in the population of Labin area in Istria, Croatia, characterized by very little population migration. METHOD:We retrospectively analyzed data on 175 patients (102 men and 73 women) diagnosed with CNS tumor according to the World Health Organ...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Materljan E,Materljan B,Sepcić J,Tuskan-Mohar L,Zamolo G,Erman-Baldini I

    更新日期:2004-04-01 00:00:00

  • Influence of 1991-1995 war on breast-feeding in Croatia: questionnaire study.

    abstract:AIM:To investigate the influence of 1991-1995 war on the prevalence, duration, and practice of breast-feeding Croatian children up to 5 years of age. METHOD:In 1996, interviews were conducted in households with children up to 2 years of age (757 children) and 2-5 years of age (1,180 children). Data for war-free areas,...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Zakanj Z,Armano G,Grgurić J,Herceg-Cavrak V

    更新日期:2000-06-01 00:00:00

  • Cross-border alliances in health care: international co-operation between health insurers and providers in the Euregio Meuse-Rhine.

    abstract::On behalf of the European Commission, a Cross-Border Health Care Project was undertaken to explore how citizens living in the Euregio Meuse-Rhine can obtain improved access to health services in the Member States concerned: Belgium, Germany, and The Netherlands. Main attention of the project is focused on practical is...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Hermans HE,den Exter A

    更新日期:1999-06-01 00:00:00

  • Neutrophil-to-lymphocyte and platelet-to-lymphocyte ratio help identify patients with lung cancer, but do not differentiate between lung cancer subtypes.

    abstract:AIM:To assess the diagnostic value of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in lung cancer (LC). We compared the ratios between healthy participants and all LC patients, as well patients with different pathohistological LC subtypes. METHODS:We retrieved the data on neutrophil, lym...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2016.57.287

    authors: Nikolić I,Kukulj S,Samaržija M,Jeleč V,Žarak M,Orehovec B,Taradi I,Romić D,Kolak T,Patrlj L

    更新日期:2016-06-30 00:00:00

  • Pregnant women quit smoking; what about fathers? Survey study in Bursa Region, Turkey.

    abstract:AIM:To evaluate maternal and paternal smoking habits during pregnancy and determine their correlation with pregnancy complications and newborn status. METHODS:The study included 499 pregnant women who delivered at the Department of Obstetrics and Gynecology in Uludag University School of Medicine, over a period of one...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Uncu Y,Ozcakir A,Ercan I,Bilgel N,Uncu G

    更新日期:2005-10-01 00:00:00

  • Development of a multiplex single base extension assay for mitochondrial DNA haplogroup typing.

    abstract:AIM:To provide a screening tool to reduce time and sample consumption when attempting mitochondrial DNA (mtDNA) haplogroup typing. METHODS:A single base primer extension assay was developed to enable typing, in a single reaction, of twelve mtDNA haplogroup specific polymorphisms. For validation purposes a total of 147...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Nelson TM,Just RS,Loreille O,Schanfield MS,Podini D

    更新日期:2007-08-01 00:00:00

  • Rebuilding the healthcare system in Mostar: challenge and opportunity.

    abstract::A basic premise of this article is that the political and ethnic division of the city of Mostar imperils community health planning efforts beyond those normally encountered in the aftermath of war. Bosnia and Herzegovina is divided among ethnic groups, and the city of Mostar is further subdivided between the Croatian ...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Pyle GF,Thompson CR,Oresković S,Bagaric I

    更新日期:1998-09-01 00:00:00

  • The incidence of postural orthostatic tachycardia syndrome in the population of Zagreb, Croatia.

    abstract:AIM:To estimate the incidence of postural orthostatic tachycardia syndrome (POTS) in the population of Zagreb, Croatia, and to determine the patients' demographic and clinical characteristics. METHODS:From 2012-2017, we identified patients with POTS by a retrospective analysis of medical records at University Hospital...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Adamec I,Crnošija L,Ruška B,Pavičić T,Junaković A,Krbot Skorić M,Pekmezović T,Habek M

    更新日期:2020-10-31 00:00:00

  • Professionals with delivery skills: backbone of the health system and key to reaching the maternal health Millennium Development Goal.

    abstract::The attainment of the fifth Millennium Development Goal requires adequate national reserves of skilled birth attendants. Nurses, midwives, and their equivalents form the frontline of the formal health system are a critical element of global efforts to reduce ill-health and poverty in the poorest areas of the world. Pl...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2008.3.318

    authors: Wirth M

    更新日期:2008-06-01 00:00:00

  • Serosurvey of human metapneumovirus infection in Croatia.

    abstract:AIM:To assess the seroprevalence of human metapneumovirus (hMPV) in Croatia. METHODS:During 2005, a total of 137 serum specimens from Croatian patients aged from 6 days to 51 years, without respiratory symptoms, were collected at the Croatian National Institute of Public Health. The sera were examined using the indire...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Ljubin Sternak S,Vilibić Cavlek T,Falsey AR,Walsh EE,Mlinarić Galinović G

    更新日期:2006-12-01 00:00:00

  • Inequality, poverty, and material deprivation in new and old members of the European Union.

    abstract:AIM:To analyze the main indicators of income inequality, objective and subjective poverty, material deprivation, and the role of public social transfers in the reduction of poverty in 15 old and 10 new member states of the European Union (EU), undergoing post-communist socio-economic transition, as well as in Croatia, ...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Matković T,Sucur Z,Zrinscak S

    更新日期:2007-10-01 00:00:00

  • Tongue somatosensory evoked potentials reflect midbrain involvement in patients with clinically isolated syndrome.

    abstract:AIM:To test the hypothesis that tSSEP findings reflect clinical and MRI MS lesions, the aim of this study was to investigate tSSEP changes in patients with clinically isolated syndrome (CIS) in relation to clinical and brainstem MRI findings. The second aim was to investigate whether the interpretation of the tSSEP res...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2016.57.558

    authors: Krbot Skorić M,Adamec I,Crnošija L,Gabelić T,Barun B,Zadro I,Butković Soldo S,Habek M

    更新日期:2016-12-31 00:00:00

  • Parental administration of antipyretics to children with upper respiratory tract infections without consultation with a physician.

    abstract:AIM:To evaluate the administration of antipyretics to children with upper respiratory tract infections (URTI) by their parents or guardians without consultation with physicians, and compare epidemiological and clinical characteristics of patients who received antipyretics and of untreated patients. METHODS:A prospecti...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2011.52.48

    authors: Andabaka T,Globočnik T,Vukelić D,Esposito S,Baršić B

    更新日期:2011-02-01 00:00:00

  • Health-related quality of life of women with disabilities in relation to their employment status.

    abstract:AIM:To compare the health-related quality of life of unemployed and employed women with disabilities and establish factors affecting their life satisfaction. METHODS:The study included 318 women with disabilities, 160 of whom were employed and 158 unemployed, paired according to age and region of residence. The health...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:10.3325/cmj.2011.52.550

    authors: Barisin A,Benjak T,Vuletić G

    更新日期:2011-08-15 00:00:00

  • Bile duct diameter changes after laparoscopic cholecystectomy: a magnetic resonance cholangiopancreatography prospective study.

    abstract:AIM:To assess the intrahepatic and extrahepatic bile duct diameter dilatation after laparoscopic cholecystectomy with magnetic resonance cholangiopancreatography. METHODS:Forty-eight patients (35 women, mean age 54.58±11.83 years) underwent laparoscopic cholecystectomy because of gallstones. The intrahepatic and extra...

    journal_title:Croatian medical journal

    pub_type: 杂志文章

    doi:

    authors: Pavlović T,Trtica S,Troskot Perić R

    更新日期:2020-07-05 00:00:00

  • A proposed role for efflux transporters in the pathogenesis of hydrocephalus.

    abstract::Hydrocephalus is a common brain disorder that is treated only with surgery. The basis for surgical treatment rests on the circulation theory. However, clinical and experimental data to substantiate circulation theory have remained inconclusive. In brain tissue and in the ventricles, we see that osmotic gradients drive...

    journal_title:Croatian medical journal

    pub_type: 杂志文章,评审

    doi:10.3325/cmj.2014.55.366

    authors: Krishnamurthy S,Tichenor MD,Satish AG,Lehmann DB

    更新日期:2014-08-28 00:00:00