Abstract：
AIM:To provide a screening tool to reduce time and sample consumption when attempting mitochondrial DNA (mtDNA) haplogroup typing. METHODS:A single base primer extension assay was developed to enable typing, in a single reaction, of twelve mtDNA haplogroup specific polymorphisms. For validation purposes a total of 147 samples were tested including 73 samples successfully haplogroup typed using mtDNA control region (CR) sequence data, 20 samples inconclusively haplogroup typed by CR sequence data, 21 samples previously haplogroup typed using RFLP analysis, and 31 samples of known ancestral origin without previous haplogroup typing. Additionally, two highly degraded human bones embalmed and buried in the early 1950s were analyzed using the SNP multiplex. RESULTS:When the SNP multiplex was used to type the 96 previously CR sequenced specimens, an increase in haplogroup or macrohaplogroup assignment relative to conventional CR sequence analysis was observed. The single base extension assay was also successfully used to assign a haplogroup to decades-old, embalmed skeletal remains dating to World War II. CONCLUSION:The SNP multiplex was successfully used to obtain haplogroup status of highly degraded human bones, and demonstrated the ability to eliminate possible contributors. The SNP multiplex provides a low-cost, high throughput method for typing of mtDNA haplogroups A, B, C, D, E, F, G, H, L1/L2, L3, M, and N that could be useful for screening purposes for human identification efforts and anthropological studies.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Nelson TM,Just RS,Loreille O,Schanfield MS,Podini Dsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
460-72issue
4eissn
0353-9504issn
1332-8166journal_volume
48pub_type
杂志文章abstract：AIM:To describe the problems in the interpretation of Croatian mortality data and explore possible reasons for the recorded increase in mortality in the 1990-1999 period, particularly related to different methods of collection and estimation of data on deaths and population. METHODS:Numbers of recorded deaths and popu...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:We present a paternity testing case in which a double incompatibility was found for two short tandem repeat (STR) markers, human fibrinogen alpha (FGA) and Penta E. Analysis of the trio (mother, father, and daughter) included the amplification with a battery of 15 autosomal short tandem repeats (STR) by using a commer...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：AIM:To evaluate maternal and paternal smoking habits during pregnancy and determine their correlation with pregnancy complications and newborn status. METHODS:The study included 499 pregnant women who delivered at the Department of Obstetrics and Gynecology in Uludag University School of Medicine, over a period of one...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：:The Faculty of Medicine in Tirana, the only institution providing medical training in Albania, has currently developed three separate branches of study, General Medicine, Dentistry, and Pharmacy. Since the early 1990s, following the collapse of the communist regime, many faculty members have been trained abroad in dif...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-02-01 00:00:00
abstract：: ...
journal_title：Croatian medical journal
pub_type： 社论,历史文章
doi：
更新日期：2018-10-31 00:00:00
abstract：AIM:To investigate missed opportunities to reveal existing but not formerly diagnosed coronary heart disease cases and related risk factors in primary health care. METHODS:The study comprised 850 people aged over 30 years with no known history of coronary heart disease, receiving health services from a primary care ce...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：AIM:To investigate the ability of two standard quality of life (QOL) questionnaires - The Short Form (36-item) Health Survey (SF-36) and The European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 (EORTC QLQ C30) to evaluate QOL in patients with chronic graft-vs-host disease (cG...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.6
更新日期：2016-02-01 00:00:00
abstract：AIM:To examine the usefulness of molecular analysis of IgH gene rearrangement in assessment of clonality in bone marrow biopsies with lymphoid aggregates (LA) and/or nodular lymphoid hyperplasia (NLH) in patients with different subtypes of malignant lymphomas. METHOD:Five hundred and twenty nine samples of bone marrow...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：AIM:To evaluate the effect of intravesical instillation of Bacillus Calmette-Guerin (BCG) in the prevention of recurrence and progression of the superficial bladder cancer. METHODS:Between February 1989 and May 1994, 170 patients with histologically proven superficial transitional cell carcinoma of the bladder stage T...
journal_title：Croatian medical journal
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:To examine ways of ensuring access to health services within the framework of primary health care (PHC), since the goal of PHC to make universal health care available to all people has become increasingly neglected amid emerging themes of globalization, trade, and foreign policy. From a public health point of view, we...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/2010.51.181
更新日期：2010-06-01 00:00:00
abstract：:Corporate medical practice, a market economy, and a consumer culture are transforming health care. The service relationships of doctors with patients are now commodities. The doctor, directed by disease management protocols (to improve outcomes, reduce costs, and standardize care), is, in effect, providing programmed ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Photolysis or "uncaging" of caged compounds represents a significant tool in cell biology and chemistry. It provides a means for quantitative control of compound delivery with temporal and spatial resolution while observing their consequences for cellular signaling. We discuss the use of ultraviolet-transmitting optic...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：AIM:To present a novel strategy for mapping quantitative trait loci (QTL), using human metapopulations. The strategy is based on the expectation that in geographic clusters of small and distinct human isolates, a combination of founder effect and genetic drift can dramatically increase population frequency of rare QTL ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-08-01 00:00:00
abstract：AIM:To present two patients with basaloid squamous cell carcinoma of the larynx, a rare, highly aggressive variant of the squamous cell carcinoma. METHODS:Surgical excision of the polypoid tumor of the anterior comissure of the larynx was performed in a 62 year-old male, and in the second case, total laryngectomy with...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：AIM:Analysis of countertransference problems in the treatment of a heterogeneous group of war veterans. METHOD:The method used in this work was psychodynamic clinical observation and analysis of countertransference phenomena in group therapy. RESULTS:In the beginning of our work, we faced with a regressive group, whi...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：AIM:To examine the feasibility of using peer review for the quality control of online materials. METHODS:We analyzed the inter-rater agreement on the quality of epidemiological lectures online, based on the Global Health Network Supercourse lecture library. We examined the agreement among reviewers by looking at kappa...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
abstract：AIM:To assess fetal behavior in all three trimesters of normal pregnancy and to investigate the continuation of behavior from fetal to neonatal period. METHODS:One hundred out of 130 pregnant women in all trimesters with singleton pregnancy were assigned for the investigation. All recordings were performed in the morn...
journal_title：Croatian medical journal
pub_type： 杂志文章,多中心研究
doi：
更新日期：2005-10-01 00:00:00
abstract：:Health and health care provision are among the most important and politically sensitive public service areas. Politicians carefully incorporate health care program changes in their political agendas to gain votes. However, knowing health care priorities of the electoral body is not useful only to politicians, but also...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：AIM:To evaluate the results of current reforms in Macedonian health sector. METHOD:Description and situation analysis, covering the period 1991-1997, are focused on demographic and vital indicators, morbidity and mortality data, elements of health care system, legislation, health insurance, health care financing, and ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：AIM:To determine whether the students enrolled in the computer-based teaching program would take the final examination in pathology earlier than those who studied according to the previous traditional program. METHODS:The study included all medical students enrolled in the pathology course at the Zagreb University Sch...
journal_title：Croatian medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2005-06-01 00:00:00
abstract：AIM:To evaluate the existing evidence on relative efficacy and tolerability of topical mono-compound intraocular pressure (IOP)-lowering drugs in treatment of primary open angle glaucoma (POAG) and ocular hypertension (OHT). METHODS:In this systematic review of systematic reviews/meta-analyses of randomized controlled...
journal_title：Croatian medical journal
pub_type： 杂志文章,评审
doi：10.3325/cmj.2014.55.468
更新日期：2014-10-01 00:00:00
abstract：AIM:To assess treatment outcomes of psychotherapy for war veterans suffering from posttraumatic stress disorder (PTSD) and to investigate self-perceived social acknowledgment. METHODS:In this prospective cohort study, a set of psychological instruments was used to assess the level of posttraumatic stress symptoms (Cli...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
abstract：AIM:To explore the economic justification for introducing ultrasound screening for developmental dysplasia of the hip in Croatia. METHODS:The analysis was based on the two formulas: that cost-benefit equals benefit/cost, and that net benefit equals benefit minus cost. Screening costs were expressed as a sum of trainin...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：AIM:Several amplification and detection formats for the analysis of short tandem repeat loci are readily available to the forensic laboratory. Careful consideration must be given to the throughput, sensitivity, concordance, data interpretation, facility requirements, and costs of operation. The Pennsylvania State Polic...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：AIM:To evaluate the effectiveness of suture anchors in the reconstruction of chronic collateral ligaments of fingers. METHODS:We treated 8 patients, 6 with chronic instability of the collateral ligament of the thumb and 2 with the instability of the fifth finger, using the Statak suture anchor. A stable joint was achi...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：AIM:To evaluate the efficacy and toxicity of preoperative chemoradiotherapy with capecitabine in locally advanced rectal cancer. METHODS:Between June 2004 and January 2005, 57 patients with operable, clinical stage II-III adenocarcinoma of the rectum entered the prospective phase II study. Radiation dose was 45 Gy (25...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-10-01 00:00:00
abstract：:Promising research developments in both basic and applied sciences, such as genomics and participatory health care approaches, have generated widespread interest in personalized medicine among almost all scientific areas and clinicians. The term personalized medicine is, however, frequently used without defining a cle...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2012.53.301
更新日期：2012-08-01 00:00:00
abstract：:Aim .To investigate if there are differences in medical ethics education between different schools of medicine in Spain, specifically between private and public schools and between recently founded schools and older ones. METHOD:The curricula of medical degrees from all Spanish faculties were reviewed for the 2014/20...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.493
更新日期：2016-10-31 00:00:00
abstract：AIM:To assess long-term mental health outcomes in people who suffer from war-related posttraumatic stress disorder (PTSD) but do not receive appropriate treatment. METHODS:We interviewed 264 subjects from former Yugoslavia, who lived in Croatia, Serbia, Germany, and the United Kingdom. All of them had suffered from PT...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2009.50.465
更新日期：2009-10-01 00:00:00
abstract：AIM:To assess the relationship between cognitive functions, severity of depressive symptoms, and expression of interleukin 1 (IL)-1 in patients treated with systemic anticancer therapy. METHODS:This prospective study, conducted in 2017-2018, involved 55 patients (56% men) subjected to systemic anticancer therapy. Fort...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2019-04-30 00:00:00
