Abstract：
:Thyroid-stimulating hormone (TSH) stimulates the synthesis and release of thyroid hormones including triiodothyronine (T3) and thyroxine (T4). Semiquantitative analyses using northern blot and in situ hybridization suggested that TSH gene transcription is upregulated under conditions of hypothyroidism. However, no quantitative analysis of TSH gene expression using real-time polymerase chain reaction (PCR) has been reported. In this study, we quantitated the TSHbeta messenger ribonucleic acid (mRNA) level as well as the TSHbeta heterogeneous nuclear ribonucleic acid (hnRNA) level in the anterior pituitary of hypothyroid rats, by real-time PCR using the LightCycler system. The hnRNA is the primary deoxyribonucleic acid (DNA) transcript, which reflects the transcription rate more reliably than the mRNA because of its short half-life. In the anterior pituitary of rats with methimazol-induced chronic hypothyroidism, both mRNA and hnRNA expression of TSHbeta were upregulated fourfold relative to normal rats (n=4). Our method provides a rapid and accurate measure of gene transcription. In the present report, we described a technique for accurate measurement of TSHbeta hnRNA level.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Sugimoto K,Mori K,Uchida K,Kobayashi D,Itoi Kdoi
10.1016/j.brainresbull.2007.06.004subject
Has Abstractpub_date
2007-09-14 00:00:00pages
142-6issue
1-3eissn
0361-9230issn
1873-2747pii
S0361-9230(07)00188-8journal_volume
74pub_type
杂志文章abstract：:After sustaining a traumatic brain injury (TBI), a person's ability to make daily decisions can be affected. Simple tasks such as, deciding what to wear are no longer effortless choices, but are instead difficult decisions. This study explored the use of a discrimination task with a magnesium treatment in order to exa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.02.019
更新日期：2018-05-01 00:00:00
abstract：:The study examines the influence of vestibular and leg proprioceptive cues on the maintenance of the body vertical in human stance. Vestibular body orientation cues were changed by applying bipolar currents to both mastoid bones (cosine-bell wave form of 3.3 s duration, 1 mA current intensity). Proprioceptive input wa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(96)00138-4
更新日期：1996-01-01 00:00:00
abstract：:This investigation assessed the relative reliability of the human auditory evoked potential (AEP) amplitude-intensity and latency-intensity functions. Averaged AEP waveforms to 10 levels of stimulus magnitude were obtained from bipolar recordings. Variability and slope of group functions were constant, as indicated by...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(80)90195-1
更新日期：1980-03-01 00:00:00
abstract：:Developmental changes in the control of neurite extension by extracellular factors can be examined by utilizing cultures of neurons from various aged embryos. Several conditioned media and tissue extracts were added to cultures of chick embryo retinal neurons on collagen substrates for 1, 3 and 5 days in vitro. Neurit...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(87)90112-2
更新日期：1987-04-01 00:00:00
abstract：:Every year, a large number of infants and young children worldwide are administered general anesthesia. Whether general anesthesia adversely affects the intellectual development and cognitive function of children at a later date remains controversial. Many animal experiments have shown that general anesthetics can cau...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2020.08.003
更新日期：2020-11-01 00:00:00
abstract：:Recent in vivo electrophysiological studies in our laboratory demonstrated medial thalamus (MT) induced short-term facilitation in the middle layers of the anterior cingulate cortex (ACC). The aim of the present study was to investigate different forms of short-term plasticity (STP) in layer II/III of the ACC in an in...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.10.011
更新日期：2007-01-09 00:00:00
abstract：:Intelligence depends on the ability of the brain to acquire and apply rules and representations. At the neuronal level these properties have been shown to critically depend on the prefrontal cortex. Here we present, in the context of the Distributed Adaptive Control architecture (DAC), a biologically based model for f...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2010.11.008
更新日期：2011-06-30 00:00:00
abstract：:The anterograde spread of herpesvirus in the visual system subsequent to retinitis has been observed clinically. We compared the ability of two well-studied Herpes simplex virus (HSV) strains to be transported in the anterograde direction in the hamster visual system: strain McIntyre, representing HSV-1, and strain 18...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(92)90038-y
更新日期：1992-03-01 00:00:00
abstract：:Previous findings showed that inhibitors of fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL), degrading enzymes of anandamide (2-AEA) and 2-arachidonoylglycerol (2-AG), reduced the nonsteroidal anti-inflammatory drug-induced gastric lesions. The present study aimed to investigate: i./whether centra...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.02.012
更新日期：2018-05-01 00:00:00
abstract：:We previously reported that a low threshold action of norepinephrine (NE) on the cerebellar circuitry is expressed as an amplification of the inhibitory action of gamma aminobutyric acid (GABA) on Purkinje cell activity. Here we examined the effects of locus coeruleus (LC) stimulation on "off-beam" inhibitions of Purk...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90211-3
更新日期：1983-06-01 00:00:00
abstract：:Theories pertaining to the "why" of motion sickness are in short supply relative to those detailing the "how." Considering the profoundly disturbing and dysfunctional symptoms of motion sickness, it is difficult to conceive of why this condition is so strongly biologically based in humans and most other mammalian and ...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2009.09.017
更新日期：2010-01-15 00:00:00
abstract：:Over the last 15 years, many publications described the use of peptide sequences that have been dubbed cell penetrating peptides (CPP), Trojan Horse peptides, protein transduction domains, or membrane-translocating sequences. These mostly positively charged domains bring attached cargo across biological membranes. One...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2005.08.015
更新日期：2005-12-15 00:00:00
abstract：:Secondary brain injuries following intracerebral hemorrhage (ICH) are mediated by inflammatory pathway activation. The present study aimed to characterize long noncoding RNAs (lncRNAs) that are differentially expressed in cerebral tissues during ICH pathogenesis and to investigate their pathogenic functions. An ICH mo...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.04.017
更新日期：2020-09-01 00:00:00
abstract：:The aim of this study was to investigate whether the histamine H1-receptor blocker, chlorpheniramine (CPA), has a reinforcing effect in goldfish. We used a place-preference procedure in an aquarium with two chambers colored black and white. On day 1, the animals were placed in the test chamber for 10 min for habituati...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(97)00287-6
更新日期：1998-01-01 00:00:00
abstract：:This is the first report of quantitative autoradiography results showing sex differences of GABAA receptor levels in brain regions of a wild rodent (wood mouse, Apodemus sylvaticus) living in its natural habitat. The labeling of this GABAergic site with its specific high affinity radioligand [3H] muscimol provided a h...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(96)00034-2
更新日期：1996-01-01 00:00:00
abstract：:Most neurodegenerative diseases are characterized by intracellular aggregates of insoluble proteins. As for the majority of these disorders, aetiology and pathogenesis are only poorly understood; current nosological concepts are largely based on these molecular signatures of protein aggregates which also provide valua...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2016.08.018
更新日期：2016-09-01 00:00:00
abstract：:Methylphenidate (MPD) is a widely prescribed psychostimulants used for the treatment of attention deficit hyperactive disorder (ADHD). Unlike the psychostimulants cocaine and amphetamine, MPD does not exhibit direct actions on the serotonin transporter, however there is evidence suggesting that the therapeutic effects...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.10.011
更新日期：2017-01-01 00:00:00
abstract：:We showed previously that the number and time of spike-wave discharges (SWDs) were increased after intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), an effect, which was completely abolished by cyclooxygenase-2 (COX-2) inhibitor indomethacin (IND) pretreatment in Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2014.03.003
更新日期：2014-05-01 00:00:00
abstract：:Glutamate was found to be an excitatory neurotransmitter in the enteric nervous system. Although several lines of evidence indicate a role of glutamate in the regulation of gut motility and secretion the physiological significance of glutamatergic transmission is not clear yet. We studied the effect of glutamate on [3...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.05.011
更新日期：2005-08-15 00:00:00
abstract：:The microdialysis method was utilized to study the effects of N omega-nitro-L-arginine (L-NNA) and N omega-nitro-D-arginine (D-NNA), an inhibitor of nitric oxide (NO) synthesis from L-arginine and its D-enantiomer, on spontaneous and N-methyl-D-aspartate (NMDA)-evoked overflow of striatal extracellular dopamine (DA) i...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90021-3
更新日期：1994-01-01 00:00:00
abstract：:Hippocampal theta activity is a large amplitude, sinusoidal wave that occurs during attentive immobility and exploratory behaviour in the rat, and it is thought to be involved in memory formation. Recent reports suggest that corticotropin-releasing hormone (CRH) has pro-mnemonic effects in rodents. Because memory-enha...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(99)00039-8
更新日期：1999-04-01 00:00:00
abstract：:The nucleus locus coeruleus (LC) has been implicated in the modulation of the spinal sensorimotor function. The aim of the present study was to examine the effect of electrical stimulation of the LC on sensorimotor function in the trigeminal system. The following two cases of sensorimotor behaviors mediated by the tri...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(00)00426-3
更新日期：2000-12-01 00:00:00
abstract：:From a psychophysiological point of view, arousal is a fundamental feature of behavior. As reported in different empirical studies based on insights from theories of consumer behavior, store atmosphere should evoke phasic arousal reactions to attract consumers. Most of these empirical investigations used verbal scales...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2005.06.012
更新日期：2005-11-15 00:00:00
abstract：:To better describe the hypothalamic efferents that mediate estrogenic effects on lordosis, each of 18 ovariectomized hamsters received a 30 gauge implant of estradiol aimed at the right or left ventromedial hypothalamus (VMN). Six days later, subjects were treated with progesterone and observed for their durations of ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(89)90094-4
更新日期：1989-04-01 00:00:00
abstract：:We investigated the likelihood that bradykinesia coexisted with levodopa-induced dyskinesias (LID) in 10 dyskinetic Parkinson's disease patients (DPD). Their motor performance was compared to that of 10 age/gender-matched non-dyskinetic patients (NDPD) and 10 healthy controls. Whole-body movement (WBM) and rapid alter...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.02.015
更新日期：2006-05-15 00:00:00
abstract：:Bilateral inferior olive lesions, produced by systemic administration of the neurotoxin 3-acetylpyridine (3AP) produce a proconvulsant state specific for strychnine-induced seizures and myoclonus. We have proposed that these phenomena are mediated through increased excitation of cerebellar Purkinje cells, through acti...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(90)90118-j
更新日期：1990-10-01 00:00:00
abstract：:Little is known about the behavioral, nutritional, and metabolic events that control dietary intake quality. Two experiments are described that manipulate nutritional conditions that have been hypothesized to affect dietary item choice so as to assess what effect, if any, the added factor of genetic obesity has in mod...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(85)90112-1
更新日期：1985-06-01 00:00:00
abstract：:A combination of immunocytochemical, electrophysiological and pharmacological techniques were employed to study the properties of neurons within the supramammillary (SUM) complex of the rat. The SUM region contains a small, but dense, population of tyrosine hydroxylase immunoreactive neurons. Following injection of th...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(88)90060-3
更新日期：1988-03-01 00:00:00
abstract：:Chronic swimming training and phytotherapeutic supplementation are assumed to alleviate oxidative damage, and support cell survival in the brain. The effect of forced, chronic swimming training, and enriched lab chow containing 1% (w/w) dried nettle (Urtica dioica) leaf were investigated for oxidative stress, inflamma...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.02.028
更新日期：2005-05-30 00:00:00
abstract：:Using immunohistochemistry for serotonin (5-HT) after administration of 5-hydroxytryptophan (5-HTP), we have demonstrated the existence of a particular group of 5-HT accumulating neurons in the posterior hypothalamus of the rat and cat. This cluster of neurons, which we termed "5-HT immunoreactive (IR) posterior hypot...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(84)90153-9
更新日期：1984-06-01 00:00:00
