Differential localization of coatomer complex isoforms within the Golgi apparatus.

abstract：:The functional architecture of mammalian visual cortex has been elucidated in impressive detail by experimental work of the past 20-25 years. The origin of many of the salient features of this architecture, however, has remained unexplained. This paper is the first of three (the others will appear in subsequent issues...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Linsker R

更新日期：1986-10-01 00:00:00

abstract：:A cDNA containing the full coding region of human cytochrome b5 was inserted into a vaccinia virus cDNA expression vector. Infection of human thymidine kinase-minus (TK-) 143 cells in culture with this recombinant virus resulted in production of 0.3 nmol of cytochrome b5 per mg of cell lysate protein. The expressed cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama T,Nagata K,Yamazoe Y,Kato R,Matsunaga E,Gelboin HV,Gonzalez FJ

更新日期：1990-07-01 00:00:00

abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

更新日期：1985-10-01 00:00:00

abstract：:The fungus, Phycomyces blakesleeanus, shows many well-defined responses to a number of external stimuli. Genetic analysis shows that at least eight genes are involved in Phycomyces sensory transduction. As a first step toward the molecular analysis of these genes and their products, we have developed a transformation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Revuelta JL,Jayaram M

更新日期：1986-10-01 00:00:00

abstract：:Swarming bacteria move in multicellular groups and exhibit adaptive resistance to multiple antibiotics. Analysis of this phenomenon has revealed the protective power of high cell densities to withstand exposure to otherwise lethal antibiotic concentrations. We find that high densities promote bacterial survival, even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler MT,Wang Q,Harshey RM

更新日期：2010-02-23 00:00:00

abstract：:III-V compound semiconductors are widely used for electronic and optoelectronic applications. However, interfacing III-Vs with other materials has been fundamentally limited by the high growth temperatures and lattice-match requirements of traditional deposition processes. Recently, we developed the templated liquid-p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hettick M,Li H,Lien DH,Yeh M,Yang TY,Amani M,Gupta N,Chrzan DC,Chueh YL,Javey A

更新日期：2020-01-14 00:00:00

abstract：:On a planet experiencing global environmental change, the governance of natural resources depends on sustained collective action by diverse populations. Engaging in such collective action can only build upon the foundation of human cognition in social-ecological settings. To help understand this foundation, we assess ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freeman J,Baggio JA,Coyle TR

更新日期：2020-04-07 00:00:00

abstract：:The two large cytoplasmic domains (C1 and C2) of adenylyl cyclases (AC), when expressed separately and mixed together, reconstitute enzyme activity that can be regulated by various modulators. Therefore, we have used the C1 or its C1a subdomain and C2 regions from type I AC (ACI) and type V AC (ACV) to identify the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wittpoth C,Scholich K,Yigzaw Y,Stringfield TM,Patel TB

更新日期：1999-08-17 00:00:00

abstract：:The human genome is made up of long DNA segments, the isochores, which are compositionally homogeneous and can be subdivided into a small number of families characterized by different G+C levels. Chromosome in situ suppression hybridization (in which excess unlabeled human DNA is added to suppress hybridization of rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saccone S,De Sario A,Wiegant J,Raap AK,Della Valle G,Bernardi G

更新日期：1993-12-15 00:00:00

abstract：:Ischemia of the myocardium and lower limbs is a common consequence of arterial disease and a major source of morbidity and mortality in modernized countries. Inducing neovascularization for the treatment of ischemia is an appealing therapeutic strategy for patients for whom traditional treatment modalities cannot be p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jang E,Albadawi H,Watkins MT,Edelman ER,Baker AB

更新日期：2012-01-31 00:00:00

abstract：:Starburst polyamidoamine dendrimers are a new class of synthetic polymers with unique structural and physical characteristics. These polymers were investigated for the ability to bind DNA and enhance DNA transfer and expression in a variety of mammalian cell lines. Twenty different types of polyamidoamine dendrimers w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kukowska-Latallo JF,Bielinska AU,Johnson J,Spindler R,Tomalia DA,Baker JR Jr

更新日期：1996-05-14 00:00:00

abstract：:The first step in poliovirus replication is binding of virus to a cellular receptor. Mouse L cells, which are resistant to poliovirus infection because they do not bear a poliovirus receptor, were transformed with HeLa cell (human) DNA to poliovirus sensitivity at a frequency of approximately 1 in 50,000 transformants...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mendelsohn C,Johnson B,Lionetti KA,Nobis P,Wimmer E,Racaniello VR

更新日期：1986-10-01 00:00:00

abstract：:Degassing of planetary interiors through surface volcanism plays an important role in the evolution of planetary bodies and atmospheres. On Earth, carbon dioxide and water are the primary volatile species in magmas. However, little is known about the speciation and degassing of carbon in magmas formed on other planets...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wetzel DT,Rutherford MJ,Jacobsen SD,Hauri EH,Saal AE

更新日期：2013-05-14 00:00:00

abstract：:Plasmacytoid dendritic cells (pDCs) are known mainly for their secretion of type I IFN upon viral encounter. We describe a CD2hiCD5+CD81+ pDC subset, distinguished by prominent dendrites and a mature phenotype, in human blood, bone marrow, and tonsil, which can be generated from CD34+ progenitors. These CD2hiCD5+CD81+...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang H,Gregorio JD,Iwahori T,Zhang X,Choi O,Tolentino LL,Prestwood T,Carmi Y,Engleman EG

更新日期：2017-02-21 00:00:00

abstract：:We show that the nonlinear mechanical response of networks formed from un-cross-linked fibrin or collagen type I continually changes in response to repeated large-strain loading. We demonstrate that this dynamic evolution of the mechanical response arises from a shift of a characteristic nonlinear stress-strain relati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Münster S,Jawerth LM,Leslie BA,Weitz JI,Fabry B,Weitz DA

更新日期：2013-07-23 00:00:00

abstract：:The RNA world hypothesis presumes that RNA will be competent for varied essential cellular functions. One such indispensable cell function is regulation of membrane permeability. Though this was not a known RNA activity, selection-amplification yielded RNAs that bound phosphatidylcholine:cholesterol liposomes. At leas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khvorova A,Kwak YG,Tamkun M,Majerfeld I,Yarus M

更新日期：1999-09-14 00:00:00

abstract：:Almost all democratic societies evolved socially and politically out of authoritarian and nondemocratic regimes. These changes not only altered the allocation of economic resources in society but also the structure of political power. In this paper, we develop a framework for studying the dynamics of political and soc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acemoglu D,Egorov G,Sonin K

更新日期：2011-12-27 00:00:00

abstract：:The most common monogenic cause of small-vessel disease leading to ischemic stroke and vascular dementia is the neurodegenerative syndrome cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), which is associated with mutations in the Notch 3 receptor. CADASIL pathology ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arboleda-Velasquez JF,Manent J,Lee JH,Tikka S,Ospina C,Vanderburg CR,Frosch MP,Rodríguez-Falcón M,Villen J,Gygi S,Lopera F,Kalimo H,Moskowitz MA,Ayata C,Louvi A,Artavanis-Tsakonas S

更新日期：2011-05-24 00:00:00

abstract：:Xenogeneic monoclonal antibodies were prepared to the murine interleukin 2 (IL-2)-dependent HT2 cell line. One rat IgM monoclonal antibody (7D4) was identified that inhibited proliferation of the HT2 cells and of IL-2-dependent CTLL cells in the presence of crude rat IL-2 as well as of purified human IL-2. The level o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malek TR,Robb RJ,Shevach EM

更新日期：1983-09-01 00:00:00

abstract：:Dimyristoyl lecithin liposomes, containing cytochrome b5 reductase (NADH:ferricytochrome b5 oxidoreductase, EC 1.6.2.2) and varying amounts of cytochrome b5, were used to measure flavoprotein catalysis alone and catalysis requiring electron transfer between the reductase and cytochrome as a function of temperature. Wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strittmatter P,Rogers MJ

更新日期：1975-07-01 00:00:00

abstract：:Class I PI3-kinases signal downstream of receptor tyrosine kinases and G protein-coupled receptors and have been implicated in tumorigenesis. Although the oncogenic potential of the PI3-kinase subunit p110α requires its mutational activation, other p110 isoforms can induce transformation when overexpressed in the wild...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dbouk HA,Pang H,Fiser A,Backer JM

更新日期：2010-11-16 00:00:00

abstract：:Goblet-cell hyperplasia is a critical pathological feature in hypersecretory diseases of airways. However, the underlying mechanisms are unknown, and no effective therapy exists. Here we show that stimulation of epidermal growth factor receptors (EGF-R) by its ligands, EGF and transforming growth factor alpha (TGFalph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeyama K,Dabbagh K,Lee HM,Agustí C,Lausier JA,Ueki IF,Grattan KM,Nadel JA

更新日期：1999-03-16 00:00:00

abstract：:Yawn contagion in humans has been proposed to be related to our capacity for empathy. It is presently unclear whether this capacity is uniquely human or shared with other primates, especially monkeys. Here, we show that in gelada baboons (Theropithecus gelada) yawning is contagious between individuals, especially thos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palagi E,Leone A,Mancini G,Ferrari PF

更新日期：2009-11-17 00:00:00

abstract：:Intracerebral hemorrhagic stroke and vascular dementia are age- and hypertension-associated manifestations of human cerebral small vessel disease (SVD). Cerebral microvessels are formed by endothelial cells (ECs), which are connected through tight junctions, adherens junctions, and stabilizing basement membrane struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinl C,Castaneda Vega S,Riehle H,Stritt C,Calaminus C,Wolburg H,Mauel S,Breithaupt A,Gruber AD,Wasylyk B,Olson EN,Adams RH,Pichler BJ,Nordheim A

更新日期：2015-08-11 00:00:00

abstract：:A fundamental problem in cell biology is to understand how spatial information is recognized and integrated into morphogenetic responses. Budding yeast undergoes differentiation to filamentous growth, which involves changes in cell polarity through mechanisms that remain obscure. Here we define a regulatory input wher...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Basu S,Vadaie N,Prabhakar A,Li B,Adhikari H,Pitoniak A,Chow J,Chavel CA,Cullen PJ

更新日期：2016-04-05 00:00:00

abstract：:RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in a form refractory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kittler R,Pelletier L,Ma C,Poser I,Fischer S,Hyman AA,Buchholz F

更新日期：2005-02-15 00:00:00

abstract：:The nucleotide cyclic di-3',5'- adenosine monophosphate (c-di-AMP) was recently identified as an essential and widespread second messenger in bacterial signaling. Among c-di-AMP-producing bacteria, altered nucleotide levels result in several physiological defects and attenuated virulence. Thus, a detailed molecular un...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huynh TN,Luo S,Pensinger D,Sauer JD,Tong L,Woodward JJ

更新日期：2015-02-17 00:00:00

abstract：:Extraction of thylakoid membranes with cholate in the presence of ammonium sulfate inactivated oxygen evolution and liberated a managanese-containing protein. This protein could be combined with preformed liposomes containing the depleted thylakoid membranes to restore 85% of the original oxygen-evolution activity. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spector M,Winget GD

更新日期：1980-02-01 00:00:00

abstract：:We have determined the volume and location of hippocampal tissue required for normal acquisition of a spatial memory task. Ibotenic acid was used to make bilateral symmetric lesions of 20-100% of hippocampal volume. Even a small transverse block (minislab) of the hippocampus (down to 26% of the total) could support sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moser MB,Moser EI,Forrest E,Andersen P,Morris RG

更新日期：1995-10-10 00:00:00

abstract：:Cell proliferation may be altered in many diseases, but it is uncertain exactly how to measure total numbers of divisions. Although it is impossible to count every division directly, potentially total numbers of stem cell divisions since birth may be inferred from numbers of somatic errors. The idea is that divisions ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim JY,Tavaré S,Shibata D

更新日期：2005-12-06 00:00:00