Abstract：
BACKGROUND:Global travellers are increasingly at risk of contracting malaria. The increasing occurrence of drug-resistance in many endemic areas emphasizes the need for novel drug targets for antimalarial-screening. In this study, the use of pyruvate kinase as a drug-target is evaluated. The functional validation of a gene encoding pyruvate kinase (designated PK1) has previously been reported. However, alternative copies of this enzyme encoded by Plasmodium falciparum could also circumvent the role of PK1. A survey of genome data revealed a putative ORF seemingly coding for another pyruvate kinase (designated PK2). METHODS:The expression of PK1 and PK2 in in vitro cultures were investigated by RT-PCR. Biocomputational analysis was carried out to identify structural differences between the P. falciparum pyruvate kinases and the corresponding enzymes from its human host. RESULTS:Both PK1 and PK2 were indeed actively transcribed during the intraerythrocytic stages, suggesting the involvement of both enzymes during infection. A comparison of amino acid residues at the effector binding sites of PK1 and PK2, to those of the human pyruvate kinases revealed some significant differences that could serve as targets for selective inhibitors to be designed against parasitic pyruvate kinases. CONCLUSION:Experimental evidence for the expression of both PK1 and PK2 during the blood stages of malaria infection was provided. Interestingly, phylogenetic analysis revealed that the "PK2" type of enzyme appears to be confined to Apicomplexans, an important observation with respect to the assessment of PK2 as a drug-target.
journal_name
Travel Med Infect Disjournal_title
Travel medicine and infectious diseaseauthors
Chan M,Tan DS,Sim TSdoi
10.1016/j.tmaid.2006.01.015subject
Has Abstractpub_date
2007-03-01 00:00:00pages
125-31issue
2eissn
1477-8939issn
1873-0442pii
S1477-8939(06)00021-4journal_volume
5pub_type
杂志文章abstract：BACKGROUND:Staphylococcus aureus is the most common cause of Skin and Soft Tissue Infections (SSTIs) in the community in the United States of America. Community Health Centers (CHC) serve as primary care providers for thousands of immigrants in New York. METHODS:As part of a research collaborative, 6 New York City-are...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2016.10.003
更新日期：2016-01-01 00:00:00
abstract：:Malaria is the most frequently imported acute, life-treating, tropical disease in international travellers. We did a literature review in PubMed using pre-defined search terms to identify possible risk factors for malaria deaths in travellers. After screening, a total of 51 papers were selected for inclusion and were ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2014.04.014
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Travellers are confronted with a variety of vector-borne threats. Is one type of repellent effective against all biting vectors? The aim of this review is to examine the literature, up to December 31st, 2012, regarding repellent efficacy. METHODS:We searched PubMed for relevant papers. Repellents of interes...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2013.10.005
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:To assess artemether-lumefantrine effectiveness in the treatment of falciparum malaria in an unsupervised outpatient setting in Africa. DESIGN:Open label prospective study. SETTING:Occupational health clinic, Mozambique. SUBJECTS:Semi-immune Mozambican adults. OUTCOME MEASURES:28-day treatment failures. ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2007.08.007
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:On 26 December 2004, the Asian tsunami hit countries around the Indian Ocean rim, particularly around its earthquake-associated epicentre off Indonesia. A number of popular tourist destinations for Australian travellers are located in this region. This study was designed to investigate travel insurance claim...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2006.01.012
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Recreational travel to wilderness destinations such as national parks is increasing. The inherent risks present in such destinations can result in injury and illness, have a significant impact on local medical resources, and end with expensive search and rescue operations. In order to increase our understand...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2008.07.002
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:The emergence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) had resulted in an unpresented global pandemic. In the initial events, the Kingdom of Saudi Arabia implemented mandatory quarantine of returning travelers in order to contain COVID-19 cases. MATERIALS AND METHODS:This is a l...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2020.101901
更新日期：2020-01-01 00:00:00
abstract：INTRODUCTION:Middle East Respiratory Coronavirus Virus (MERS-CoV) first emerged from Saudi Arabia in 2012 and has since been recognized as a significant human respiratory pathogen on a global level. METHODS:In this narrative review, we focus on the prevention of MERS-CoV. We searched PubMed, Embase, Cochrane, Scopus, ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2019.101520
更新日期：2019-11-12 00:00:00
abstract：:Air travel during the COVID-19 pandemic is challenging for travellers, airlines, airports, health authorities, and governments. We reviewed multiple aspects of COVID peri-pandemic air travel, including data on traveller numbers, peri-flight prevention, and testing recommendations and in-flight SARS-CoV-2 transmission,...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2020.101939
更新日期：2020-01-01 00:00:00
abstract：UNLABELLED:Our objective was to determine tuberculin skin test conversion rate of health care workers traveling to Botswana. The rate of tuberculin skin test conversion was 4.2% for the entire group studied or 6.87 per 1000 person weeks (95% CI, 1.87-17.60). BACKGROUND:International travel by health care workers trave...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2013.07.002
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:Cruise ships carry a large number of people in confined spaces providing an environment for transmission of infections. The aim of this study is to estimate the incidence of and describe the spectrum of respiratory infections and gastrointestinal illness among passengers and crew of cruise Ship A. MATERIAL ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2016.05.019
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:Leishmania species that occur within different geographical areas may cause different clinical manifestations, virulence and drug sensitivity. Patients/Methods. All patients with a clinical diagnosis of cutaneous leishmaniasis seen at the Hospital for Tropical Diseases from 1997 to 2000 were identified and c...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2004.11.002
更新日期：2006-01-01 00:00:00
abstract：:Influenza and meningococcal disease are two serious diseases that are especially linked. Outbreaks of influenza have been frequently associated with secondary outbreaks of meningococcal disease. Travellers such as Hajj pilgrims are at particular risk, the most recent meningococcal outbreaks being in 2000 and 2001, whi...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2008.09.001
更新日期：2009-07-01 00:00:00
abstract：BACKGROUND:Traveller's diarrhoea (TD) is the most common infectious problem for travellers, and we lack effective ways of preventing it, apart from antibiotic prophylaxis. This study aims to quantify the risk reduction of a hypothetic TD vaccine Norwegian travellers are willing to pay for. METHODS:1204 clients at Reis...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2016.10.005
更新日期：2016-01-01 00:00:00
abstract：:Travellers with recognised disabilities or the dis-ability to function as required during a trip have been overlooked in the travel medicine literature. This paper provides a starting point for further discussion and research into this neglected traveller population. In contrast, tourism research has explored travel w...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2018.02.001
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Rickettsial diseases present as acute febrile illnesses, sometimes with inoculation eschars. METHODS:We performed a systematic review of studies published between 1997 and 2017 to assess the underestimation of non-eschar rickettsial disease (NERD) relative to eschar rickettsial disease (ERD), as a cause of ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2018.02.006
更新日期：2018-01-01 00:00:00
abstract：:The Global Polio Eradication Initiative (GPEI) in Pakistan has faced failure despite being implemented successfully. Polio cases were successfully reduced by 99% until 2005. However, thereafter, new polio cases were registered, which continue to rise annually. This repeat polio outbreak has placed the country on watch...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2015.06.004
更新日期：2015-09-01 00:00:00
abstract：:At present there are no 'high-tech' solutions to the problems that may beset intercontinental travellers. Indications for the use of drugs are limited, and their use must accord with Good Clinical Practice. Essentially, travellers must look after their sleep, as far as possible, during and after the flight. The most u...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2006.01.009
更新日期：2006-12-01 00:00:00
abstract：BACKGROUND:Data on imported malaria in pregnant women are scarce. METHOD:A retrospective, descriptive study of pooled data on imported malaria in pregnancy was done using data from 1991 to 2014 from 8 different collaborators in Europe, the United States and Japan. National malaria reference centres as well as speciali...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2015.06.011
更新日期：2015-07-01 00:00:00
abstract：:Cutaneous myiasis caused by Dermatobia hominis, the human botfly, involves the infestation of human tissue with fly larvae, and is common in Central and South America. We report a case of a 57-year-old man with cutaneous myiasis imported into the US from Belize. The epidemiology, biological life cycle, clinical presen...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2006.09.005
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:In an initial study among children from non-Japanese encephalitis (JE)-endemic countries, seroprotection rates remained high 6 months after completion of the primary series with IXIARO®. METHODS:In this open-label follow-up study, a subset of 23 children who received a 2-dose primary series of IXIARO® in th...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2020.101616
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:The diagnosis of acute malaria in non-endemic countries is still carried out largely by microscopic examination of thick and thin smears or rapid diagnostic tests. Low-density infections might be missed, however, but the more sensitive PCR is more expensive, complex and requires considerable more time. METH...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2014.12.016
更新日期：2015-03-01 00:00:00
abstract：BACKGROUND:Children travel with their families, including children with chronic illness. We know that adults with chronic illness who travel are more likely than their healthy peers to become sick while traveling. A review of the literature was undertaken to identify what is known about traveling with children with spe...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.tmaid.2019.06.009
更新日期：2020-01-01 00:00:00
abstract：:International travel is changing the epidemiology of imported malaria. Our aim was to study malaria prophylaxis administered to travellers from Greece. The study was conducted during 2008-2009. Data were collected using a standardized form. A total of 2337 travellers were studied; prophylaxis was recommended to 60.2% ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2011.09.005
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:In France, no previous studies have focused specifically on health problems among medical students during internships abroad including the clinical symptoms suggestive of infectious diseases and the acquisition of pathogen carriage. METHODS:Clinical follow up and qPCR based respiratory, gastrointestinal and...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2019.101548
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:To investigate the genetic diversity of Haemophilus influenzae positive samples among French pilgrims attending the 2018 Hajj pilgrimage. METHOD:After screening by qPCR, multilocus sequence typing was performed for all H. influenzae-positive samples. The following housekeeping genes were amplified and assig...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2020.101883
更新日期：2020-01-01 00:00:00
abstract：:Recent case reports of viral hemorrhagic fever in Europe and the United States have raised concerns about the possibility for increased importation of filoviruses to non-endemic areas. This emerging threat is concerning because of the increase in global air travel and the rise of tourism in central and eastern Africa ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2010.05.003
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:The objective of this study was to describe the clinical pathology and seasonal variation of cutaneous larva migrans (CLM) in an impoverished community. METHOD:A representative sample of the population of a poor urban neighbourhood in Fortaleza, northeast Brazil, was examined for CLM four times during a per...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2003.10.003
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND:Currently, Brazil is experiencing one of the fastest increasing coronavirus disease (COVID-19) mortality rates worldwide, with a minimum of 158,000 confirmed deaths presently. The city of São Paulo is particularly vulnerable because it is the most populated city in Brazil. Thus, this study aimed to analyse C...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2020.101945
更新日期：2020-12-02 00:00:00
abstract：BACKGROUND:International travel is considered a risk for colonisation with extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE). To our knowledge, no studies to date have focused on ESBL-PE colonisation among long-term business travellers. Therefore this study aimed to clarify the characteristics associ...
journal_title：Travel medicine and infectious disease
pub_type： 杂志文章
doi：10.1016/j.tmaid.2016.09.012
更新日期：2016-01-01 00:00:00
