Diazonamide toxins reveal an unexpected function for ornithine delta-amino transferase in mitotic cell division.

abstract：:For a series of different proteins, including a structural protein, enzyme, inhibitor, protein marker, and a charge-transfer system, we have quantified the higher affinity of Na+ over K+ to the protein surface by means of molecular dynamics simulations and conductivity measurements. Both approaches show that sodium bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vrbka L,Vondrásek J,Jagoda-Cwiklik B,Vácha R,Jungwirth P

更新日期：2006-10-17 00:00:00

abstract：:Interleukin 2 (IL-2) plays a critical role in the growth and differentiation of lymphoid cells. The IL-2 signal is delivered intracellularly by the IL-2 receptor beta chain (IL-2R beta); however, the mechanism by which the signal reaches the nucleus remains unclear. In this study, we demonstrate the rapid activation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hatakeyama M,Kawahara A,Mori H,Shibuya H,Taniguchi T

更新日期：1992-03-15 00:00:00

abstract：:A key component of scientific communication is sufficient information for other researchers in the field to reproduce published findings. For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stodden V,Seiler J,Ma Z

更新日期：2018-03-13 00:00:00

abstract：:The enzyme p-cresol methylhydroxylase [4-cresol: (acceptor) oxidoreductase (methyl-hydroxylating), EC 1.17.99.1] contains two subunits: a cytochrome c (electron transfer) subunit (cytochrome cpc) and a flavin (catalytic) subunit. When these subunits are separated by isoelectric focusing, a stable cytochrome subunit is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLendon GL,Bagby S,Charman JA,Driscoll PC,McIntire WS,Mathews FS,Hill HA

更新日期：1991-11-01 00:00:00

abstract：:Capsaicin (CP), the pungent component of chili pepper, acts on sensory neurons to convey the sensation of pain. The CP receptor, vanilloid receptor 1 (VR1), has been shown to be highly expressed by nociceptive neurons in dorsal root and trigeminal ganglia. We demonstrate here that the dendritic cell (DC), a key cell t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Basu S,Srivastava P

更新日期：2005-04-05 00:00:00

abstract：:A mutation in the Escherichia coli dsbA gene (coding for a periplasmic disulfide oxidoreductase) reduces the rate of disulfide bond formation in the enzyme pullulanase and also reduces the rate at which the enzyme is secreted to the cell surface, as measured by protease accessibility. The enzyme did not become proteas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pugsley AP

更新日期：1992-12-15 00:00:00

abstract：:Secondary (i.e., heterotrophic or animal) production is a main pathway of energy flow through an ecosystem as it makes energy available to consumers, including humans. Its estimation can play a valuable role in the examination of linkages between ecosystem functions and services. We found that oil and gas platforms of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Claisse JT,Pondella DJ 2nd,Love M,Zahn LA,Williams CM,Williams JP,Bull AS

更新日期：2014-10-28 00:00:00

abstract：:In a genetic screen of available T-DNA-mutagenized Arabidopsis populations for loci potentially involved in phytochrome (phy) signaling, we identified a mutant that displayed reduced seedling deetiolation under continuous red light, but little if any change in responsiveness to continuous far-red light. This behavior ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huq E,Tepperman JM,Quail PH

更新日期：2000-08-15 00:00:00

abstract：:Although aminoacyl-tRNA synthetases (ARSs) are essential for protein synthesis, they also function as regulators and signaling molecules in diverse biological processes. Here, we screened 11 different human ARSs to identify the enzyme that is secreted as a signaling molecule. Among them, we found that lysyl-tRNA synth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park SG,Kim HJ,Min YH,Choi EC,Shin YK,Park BJ,Lee SW,Kim S

更新日期：2005-05-03 00:00:00

abstract：:Yeast mutants in which genes encoding subunits of the vacuolar H(+)-ATPase were interrupted were assayed for their vacuolar ATPase and proton-uptake activities. The vacuoles from the mutants lacking subunits A (72 kDa), B (57 kDa), or c (proteolipid, 16 kDa) were completely inactive in these reactions. Immunological s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noumi T,Beltrán C,Nelson H,Nelson N

更新日期：1991-03-01 00:00:00

abstract：:The hetR gene plays a very important role in cell differentiation of heterocystous cyanobacteria. To understand the mechanism of the hetR gene product in regulation of heterocyst differentiation, the recombinant HetR protein (rHetR) was overproduced in Escherichia coli. Purified rHetR was unstable and degraded easily ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou R,Wei X,Jiang N,Li H,Dong Y,Hsi KL,Zhao J

更新日期：1998-04-28 00:00:00

abstract：:Calcicludine (CaC) is a 60-amino acid polypeptide from the venom of Dendroaspis angusticeps. It is structurally homologous to the Kunitz-type protease inhibitor, to dendrotoxins, which block K+ channels, and to the protease inhibitor domain of the amyloid beta protein that accumulates in Alzheimer disease. Voltage-cla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schweitz H,Heurteaux C,Bois P,Moinier D,Romey G,Lazdunski M

更新日期：1994-02-01 00:00:00

abstract：:Habituation is a form of simple memory that suppresses neural activity in response to repeated, neutral stimuli. This process is critical in helping organisms guide attention toward the most salient and novel features in the environment. Here, we follow known circuit mechanisms in the fruit fly olfactory system to der...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen Y,Dasgupta S,Navlakha S

更新日期：2020-06-02 00:00:00

abstract：:Use-dependent long-term changes of neuronal response properties must be gated to prevent irrelevant activity from inducing inappropriate modifications. Here we test the hypothesis that local network dynamics contribute to such gating. As synaptic modifications depend on temporal contiguity between presynaptic and post...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galuske RAW,Munk MHJ,Singer W

更新日期：2019-11-12 00:00:00

abstract：:Suppressor of variegation 3-9 homolog 1 (SUV39H1), a histone methyltransferase, catalyzes histone 3 lysine 9 trimethylation and is involved in heterochromatin organization and genome stability. However, the mechanism for regulation of the enzymatic activity of SUV39H1 in cancer cells is not yet well known. In this stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang D,Zhou J,Liu X,Lu D,Shen C,Du Y,Wei FZ,Song B,Lu X,Yu Y,Wang L,Zhao Y,Wang H,Yang Y,Akiyama Y,Zhang H,Zhu WG

更新日期：2013-04-02 00:00:00

abstract：:High vein density, a distinctive trait of C4 leaves, is central to both C3-to-C4 evolution and conversion of C3 to C4-like crops. We tested the hypothesis that high vein density in C4 leaves is due to elevated auxin biosynthesis and transport in developing leaves. Up-regulation of genes in auxin biosynthesis pathways ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CF,Yu CP,Wu YH,Lu MJ,Tu SL,Wu SH,Shiu SH,Ku MSB,Li WH

更新日期：2017-08-15 00:00:00

abstract：:Recent studies with fibroblasts have shown that peptidylhydroxyproline formation begins toward the end of the log phase of growth and is an expression of the activity of the enzyme, collagen proline hydroxylase, which increase in late log-phase cells. This communication reports a two- to fivefold increase in hydroxyla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Comstock JP,Udenfriend S

更新日期：1970-06-01 00:00:00

abstract：:A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)-expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A beta-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li XH,Carrington JC

更新日期：1995-01-17 00:00:00

abstract：:Plants and animals deploy intracellular immune receptors that perceive specific pathogen effector proteins and microbial products delivered into the host cell. We demonstrate that the ADR1 family of Arabidopsis nucleotide-binding leucine-rich repeat (NB-LRR) receptors regulates accumulation of the defense hormone sali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonardi V,Tang S,Stallmann A,Roberts M,Cherkis K,Dangl JL

更新日期：2011-09-27 00:00:00

abstract：:Understanding the relationship between genetic variation and phenotypic variation for quantitative traits is necessary for predicting responses to natural and artificial selection and disease risk in human populations, but is challenging because of large sample sizes required to detect and validate loci with small eff...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swarup S,Huang W,Mackay TF,Anholt RR

更新日期：2013-01-15 00:00:00

abstract：:The envelope spike of HIV is one of the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles in infection and immune evasion, the chemical structures of the glycans on the native viral envelope glycoprotein gp120--as opposed to recombinantly ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doores KJ,Bonomelli C,Harvey DJ,Vasiljevic S,Dwek RA,Burton DR,Crispin M,Scanlan CN

更新日期：2010-08-03 00:00:00

abstract：:Previous studies have provided evidence that IL-15 expression within human tumors is crucial for optimal antitumor responses; however, the regulation of IL-15 within the tumor microenvironment (TME) is unclear. We report herein, in analyses of mice implanted with various tumor cell lines, soluble IL-15/IL-15Rα complex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santana Carrero RM,Beceren-Braun F,Rivas SC,Hegde SM,Gangadharan A,Plote D,Pham G,Anthony SM,Schluns KS

更新日期：2019-01-08 00:00:00

abstract：:Previously we reported that pretreatment of rats with the substance P (SP) antagonist CP-96,345 inhibits the enterotoxic responses following administration of toxin A from Clostridium difficile into ileal loops, indicating that SP participates in the intestinal responses to this toxin. We now report that injection of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castagliuolo I,Keates AC,Qiu B,Kelly CP,Nikulasson S,Leeman SE,Pothoulakis C

更新日期：1997-04-29 00:00:00

abstract：:Early events in the action of 17-beta-estradiol can be studied in soluble extracts of rat uterus by exposure of the estradiol-receptor protein to a DNA-cellulose matrix. After complexing with [(3)H]estradiol, the 4S receptor protein binds to the DNA, and it can be eluted with buffer of high ionic strength as a more ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto KR,Alberts BM

更新日期：1972-08-01 00:00:00

abstract：:Seemingly similar individuals often experience drastically different success trajectories, with some repeatedly failing and others consistently succeeding. One explanation is preexisting variability along unobserved fitness dimensions that is revealed gradually through differential achievement. Alternatively, positive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van de Rijt A,Kang SM,Restivo M,Patil A

更新日期：2014-05-13 00:00:00

abstract：:The subdivision of all the experimental days of the Whitetop project into two approximately equal groups, group W with predominantly westerly winds aloft and group E with frequent easterly winds, shows a remarkable difference in the apparent effect of seeding. On W days there was no detectable effect of seeding on rai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lovasich JL,Neyman J,Scott EL,Smith JA

更新日期：1969-11-01 00:00:00

abstract：:Methods to acutely manipulate protein interactions at the subcellular level are powerful tools in cell biology. Several blue-light-dependent optical dimerization tools have been developed. In these systems one protein component of the dimer (the bait) is directed to a specific subcellular location, while the other com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benedetti L,Barentine AES,Messa M,Wheeler H,Bewersdorf J,De Camilli P

更新日期：2018-03-06 00:00:00

abstract：:Bacterial RNases catalyze the turnover of RNA and are essential for gene expression and quality surveillance of transcripts. In Escherichia coli, the exoribonucleases RNase R and polynucleotide phosphorylase (PNPase) play critical roles in degrading RNA. Here, we developed an optical-trapping assay to monitor the tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fazal FM,Koslover DJ,Luisi BF,Block SM

更新日期：2015-12-08 00:00:00

abstract：:The Belgrade (b) rat has an autosomal recessively inherited, microcytic, hypochromic anemia associated with abnormal reticulocyte iron uptake and gastrointestinal iron absorption. The b reticulocyte defect appears to be failure of iron transport out of endosomes within the transferrin cycle. Aspects of this phenotype ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleming MD,Romano MA,Su MA,Garrick LM,Garrick MD,Andrews NC

更新日期：1998-02-03 00:00:00

abstract：:The type of cytokines produced during T cell responses determines susceptibility or resistance to many pathogens and influences the development of autoimmunity and allergy. To define the role of individual accessory molecules in cytokine production during primary immune responses, Drosophila cell lines expressing muri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luksch CR,Winqvist O,Ozaki ME,Karlsson L,Jackson MR,Peterson PA,Webb SR

更新日期：1999-03-16 00:00:00