Towards elucidation of the mechanism of UV1C, a deoxyribozyme with photolyase activity.

abstract：:Rabbit psoas muscle filaments, isolated in relaxing buffer from non-glycerinated muscle, have been applied to hydrophilic carbon films and stained with uranyl acetate. Electron micrographs were obtained under low-dose conditions to minimize specimen damage. Surrounding the filament backbone, except in the bare zone, i...

journal_title：Journal of molecular biology

authors： Knight P,Trinick J

更新日期：1984-08-15 00:00:00

abstract：:The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an excised Ssp DnaE intein and the second from a splicing-deficient precu...

journal_title：Journal of molecular biology

authors： Sun P,Ye S,Ferrandon S,Evans TC,Xu MQ,Rao Z

更新日期：2005-11-11 00:00:00

abstract：:The SH3 domain is a protein-protein interaction module commonly found in intracellular signaling and adaptor proteins. The SH3 domains of multiple endocytic proteins have been recently implicated in binding ubiquitin, which serves as a signal for diverse cellular processes including gene regulation, endosomal sorting,...

journal_title：Journal of molecular biology

authors： He Y,Hicke L,Radhakrishnan I

更新日期：2007-10-12 00:00:00

abstract：:Cdc25B is a dual specificity phosphatase involved in the control of cyclin-dependent kinases and the progression of cells through the cell cycle. A series of minimal domain Cdc25B constructs maintaining catalytic activity have been expressed. The structure of a minimum domain construct binding sulfate was determined a...

journal_title：Journal of molecular biology

authors： Reynolds RA,Yem AW,Wolfe CL,Deibel MR Jr,Chidester CG,Watenpaugh KD

更新日期：1999-10-29 00:00:00

abstract：:New methods for collecting cross-relaxation data from proteins and nucleic acids make it possible to improve the accuracy and precision of interproton distance measurements used as input for NMR solution structure determinations. It thus is of interest to determine whether such experimentally achievable improvements i...

journal_title：Journal of molecular biology

authors： Hoogstraten CG,Markley JL

更新日期：1996-05-03 00:00:00

abstract：:The insertion sequence IS903 encodes a 307 amino acid residue protein, transposase, that is essential for transposition. It is a multi-functional DNA-binding protein that specifically recognizes the 18 bp inverted repeats at the ends of the element and also recognizes DNa non-specifically when it captures a target sit...

journal_title：Journal of molecular biology

authors： Tavakoli NP,DeVost J,Derbyshire KM

更新日期：1997-12-12 00:00:00

abstract：:Previous kinetic studies demonstrated that nucleotide-derived conformational changes regulate function in the COOH-terminal Src kinase. We have employed enhanced methods of hydrogen-deuterium exchange-mass spectrometry (DXMS) to probe conformational changes on CSK in the absence and presence of nucleotides and thereby...

journal_title：Journal of molecular biology

authors： Hamuro Y,Wong L,Shaffer J,Kim JS,Stranz DD,Jennings PA,Woods VL Jr,Adams JA

更新日期：2002-11-08 00:00:00

abstract：:Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have shown that in addition to the active site, pyridoxine 5'-phosphate oxidase contains a non-catalyti...

journal_title：Journal of molecular biology

authors： Safo MK,Musayev FN,di Salvo ML,Schirch V

更新日期：2001-07-20 00:00:00

abstract：:Expression of the Caenorhabditis elegans cysteine protease gene cpr-1 is regulated both spatially and temporally. In situ hybridisation and Northern blot analysis have shown that this gene is expressed exclusively in gut cells of all developmental stages except the embryo. We now show by transgenic transformation with...

journal_title：Journal of molecular biology

authors： Britton C,McKerrow JH,Johnstone IL

更新日期：1998-01-01 00:00:00

abstract：:Low dose electron diffraction and imaging techniques have been applied to the study of the crystalline structure of gp32*I, a DNA helix destabilizing protein derived from bacteriophage T4 gene 32 protein. A quantitative analysis of intensities from electron diffraction patterns from tilted, multilayered gp32*I crystal...

journal_title：Journal of molecular biology

authors： Cohen HA,Chiu W,Hosoda J

更新日期：1983-09-05 00:00:00

abstract：:Protein self-assembly is a common and essential biological phenomenon, and bacterial microcompartments present a promising model system to study this process. Bacterial microcompartments are large, protein-based organelles which natively carry out processes important for carbon fixation in cyanobacteria and the surviv...

journal_title：Journal of molecular biology

authors： Kennedy NW,Ikonomova SP,Slininger Lee M,Raeder HW,Tullman-Ercek D

更新日期：2021-01-22 00:00:00

abstract：:The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature chitobiase ArChb possesses four functionally independent domains: a catalytic domain stabilized by Ca(2+), a galactose-bindi...

journal_title：Journal of molecular biology

authors： Lonhienne T,Zoidakis J,Vorgias CE,Feller G,Gerday C,Bouriotis V

更新日期：2001-07-06 00:00:00

abstract：:We have evaluated the thermodynamic parameters associated with cooperative cofilin binding to actin filaments, accounting for contributions of ion-linked equilibria, and determined the kinetic basis of cooperative cofilin binding. Ions weaken non-contiguous (isolated, non-cooperative) cofilin binding to an actin filam...

journal_title：Journal of molecular biology

authors： Cao W,Goodarzi JP,De La Cruz EM

更新日期：2006-08-11 00:00:00

abstract：:Muscular dystrophies arise with various mutations in dystrophin, implicating this protein in force transmission in normal muscle. With 24 three-helix, spectrin repeats interspersed with proline-rich hinges, dystrophin's large size is an impediment to gene therapy, prompting the construction of mini-dystrophins. Result...

journal_title：Journal of molecular biology

authors： Bhasin N,Law R,Liao G,Safer D,Ellmer J,Discher BM,Sweeney HL,Discher DE

更新日期：2005-09-30 00:00:00

abstract：:Dimers of the Rel/NFkappaB transcription factor family form with differential stabilities through the combinatorial association of five polypeptides: p50, p52, p65, cRel, and RelB. Here, we have characterized the nature of the monomer-dimer equilibrium of the p50 homodimer. Sedimentation equilibrium studies show that ...

journal_title：Journal of molecular biology

authors： Sengchanthalangsy LL,Datta S,Huang DB,Anderson E,Braswell EH,Ghosh G

更新日期：1999-06-18 00:00:00

abstract：:Aquaporin-4 (AQP4) is the predominant water channel in the mammalian brain and an important drug target for treatment of cerebral edema, bipolar disorder and mesial temporal lobe epilepsy. We determined the AQP4 structure by electron crystallography of double-layered, two-dimensional (2D) crystals. The structure allow...

journal_title：Journal of molecular biology

authors： Hiroaki Y,Tani K,Kamegawa A,Gyobu N,Nishikawa K,Suzuki H,Walz T,Sasaki S,Mitsuoka K,Kimura K,Mizoguchi A,Fujiyoshi Y

更新日期：2006-01-27 00:00:00

abstract：:The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) functions in splicing group I introns by promoting the formation of the catalytically active structure of the intron's catalytic core. Previous studies suggested a model in which the protein binds first to the intron's P4-P6 domain, and then ...

journal_title：Journal of molecular biology

authors： Caprara MG,Myers CA,Lambowitz AM

更新日期：2001-04-27 00:00:00

abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

journal_title：Journal of molecular biology

authors： Herzberg O

更新日期：1991-02-20 00:00:00

abstract：:Crystal structures of an Fab (NC6.8) from a murine IgG2b(kappa) antibody and its complex with a sweet-tasting, N-,N'-,N"-trisubstituted guanidine compound (NC174) have been determined by X-ray analysis. Both crystal forms are produced by a microseeding technique in polyethylene glycol (PEG) 8000 but the habits and spa...

journal_title：Journal of molecular biology

authors： Guddat LW,Shan L,Anchin JM,Linthicum DS,Edmundson AB

更新日期：1994-02-11 00:00:00

abstract：:Insertion and deletion mutagenesis within the gene topA of Escherichia coli encoding DNA topoisomerase I was carried out to test the existence of subdomains in the enzyme and the relationship between the slow-growth topA- phenotype and the known DNA relaxation activity of the enzyme. All mutants that show no detectabl...

journal_title：Journal of molecular biology

authors： Zumstein L,Wang JC

更新日期：1986-10-05 00:00:00

abstract：:Pseudomonas aeruginosa encodes an enzyme (PA2794) that is annotated as a sialidase (or neuraminidase), as it possesses three bacterial neuraminidase repeats that are a signature of nonviral sialidases. A recent report showed that when the gene encoding this sialidase is knocked out, this led to a reduction in biofilm ...

journal_title：Journal of molecular biology

authors： Xu G,Ryan C,Kiefel MJ,Wilson JC,Taylor GL

更新日期：2009-02-27 00:00:00

abstract：:The Rad1-Rad9-Hus1 (9-1-1) complex serves a dual role as a DNA-damage sensor in checkpoint signaling and as a mediator in the DNA repair pathway. However, the intercellular mechanisms that regulate the 9-1-1 complex are poorly understood. Jab1, the fifth component of the COP9 signalosome complex, has a central role in...

journal_title：Journal of molecular biology

authors： Huang J,Yuan H,Lu C,Liu X,Cao X,Wan M

更新日期：2007-08-10 00:00:00

abstract：:Integration host factor (IHF) of Escherichia coli is a histone-like protein that is involved both in site-specific recombination and in regulating the expression of a number of phage and bacterial genes. We have shown previously that transcription of the ilvGMEDA operon in E. coli is greatly reduced in IHF mutants. We...

journal_title：Journal of molecular biology

authors： Tsui P,Freundlich M

更新日期：1988-10-05 00:00:00

abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

journal_title：Journal of molecular biology

authors： Birkeland NK,Lindquist BH

更新日期：1986-04-05 00:00:00

abstract：:Ribgrass mosaic virus (RMV) is a member of the tobamovirus group of plant viruses. The structure has been determined at 2.9 A resolution by fiber diffraction methods, and refined by molecular dynamics methods to an R-factor of 0.095. The carboxyl-carboxylate interactions that drive disassembly in tobamoviruses are pre...

journal_title：Journal of molecular biology

authors： Wang H,Culver JN,Stubbs G

更新日期：1997-06-27 00:00:00

abstract：:The replacement of cis proline 39 of ribonuclease T1 by an alanine residue leads to a decrease in stability by about 20 kJ/mol and to major changes in the folding kinetics that are not easily explained by the proline model for protein folding. In particular, a novel very slow reaction is observed in the refolding of t...

journal_title：Journal of molecular biology

authors： Mayr LM,Schmid FX

更新日期：1993-06-05 00:00:00

abstract：:Several experimental data support the notion that the recognition of DNA crossovers play an important role in the multiple functions of topoisomerase II. Here, a theoretical analysis of the possible modes of assembly of yeast topoisomerase II with right and left-handed tight DNA crossovers is performed, using the crys...

journal_title：Journal of molecular biology

authors： Timsit Y,Duplantier B,Jannink G,Sikorav JL

更新日期：1998-12-18 00:00:00

abstract：:The small GTPases Arf1 and Arf6 have nonoverlapping functions in cellular traffic despite their very high sequence and structural resemblance. Notably, the exquisite isoform specificity of their guanine nucleotide exchange factors and their distinctive sensitivity to the drug brefeldin A cannot be explained by any str...

journal_title：Journal of molecular biology

authors： Biou V,Aizel K,Roblin P,Thureau A,Jacquet E,Hansson S,Guibert B,Guittet E,van Heijenoort C,Zeghouf M,Perez J,Cherfils J

更新日期：2010-10-01 00:00:00

abstract：:Huntington's disease (HD) is an inherited progressive neurodegenerative disorder caused by a pathological CAG trinucleotide repeat expansion in the large multi-exon gene, huntingtin (HTT). Although multiple pathogenic mechanisms have been proposed for HD, there is increasing interest in the RNA processing of the HTT g...

journal_title：Journal of molecular biology

authors： Hughes AC,Mort M,Elliston L,Thomas RM,Brooks SP,Dunnett SB,Jones L

更新日期：2014-04-03 00:00:00

abstract：:Here, we report the first characterization and partial purification of an archaeal tRNA 3' processing activity, the RNase Z from Haloferax volcanii. The activity identified here is an endonuclease, which cleaves tRNA precursors 3' to the discriminator. Thus tRNA 3' processing in archaea resembles the eukaryotic 3' pro...

journal_title：Journal of molecular biology

authors： Schierling K,Rösch S,Rupprecht R,Schiffer S,Marchfelder A

更新日期：2002-03-01 00:00:00