tRNA 3' end maturation in archaea has eukaryotic features: the RNase Z from Haloferax volcanii.

abstract：:It is now appreciated that in addition to their role in humoral immunity, B cells also exert regulatory mechanisms that lead to attenuation of inflammatory responses. The concept of B-cell regulation became well recognized when mice deficient in B cells due to genetic disruption were shown to be refractory to recovery...

journal_title：Journal of molecular biology

authors： Khalil MI,Gurski CJ,Dittel LJ,Neu SD,Dittel BN

更新日期：2021-01-08 00:00:00

abstract：:The chicken nuclear lamina is composed of at least three proteins called lamins A, B1 and B2. In addition, putative precursors are transiently expressed during in vivo synthesis of lamins A and B2. Here we report the complete sequence of lamin B2 as it is deduced from a cloned cDNA. Comparison of lamin B2 with lamins ...

journal_title：Journal of molecular biology

authors： Vorburger K,Lehner CF,Kitten GT,Eppenberger HM,Nigg EA

更新日期：1989-08-05 00:00:00

abstract：:In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. The autoinducer-2 production protein LuxS, is involved in a novel quorum-sensing system and is thought to catalyse the degradation of S-ribosylhomocysteine to homocysteine and the autoinducer molecule 4,5-di...

journal_title：Journal of molecular biology

authors： Ruzheinikov SN,Das SK,Sedelnikova SE,Hartley A,Foster SJ,Horsburgh MJ,Cox AG,McCleod CW,Mekhalfia A,Blackburn GM,Rice DW,Baker PJ

更新日期：2001-10-12 00:00:00

abstract：:Factor B is a key component of the alternative pathway of complement and is cleaved by factor D into the Ba and Bb fragments in the presence of activated C3 (C3b or C3(H(2)O)). The Ba fragment contains three short consensus/complement repeat domains, while the Bb fragment contains a von Willebrand factor type A (vWF-A...

journal_title：Journal of molecular biology

authors： Hinshelwood J,Perkins SJ

更新日期：2000-09-01 00:00:00

abstract：:The structure of cardiotoxin CTX I from Naja naja atra has been investigated by NMR spectroscopy. Sequence specific resonance assignments have been obtained for all backbone protons as well as for most side-chain protons. Distance geometry calculations were carried out using a metric matrix DG program. A total of 715 ...

journal_title：Journal of molecular biology

authors： Jahnke W,Mierke DF,Béress L,Kessler H

更新日期：1994-07-29 00:00:00

abstract：:Oncogenes may play a crucial role in the genetic program of cellular differentiation; even, probably, at a very early stage in this program, which can be described as pre-commitment. We have investigated the variation in, and the control level of, the accumulation of the transformation-associated cellular protein p53 ...

journal_title：Journal of molecular biology

authors： Khochbin S,Principaud E,Chabanas A,Lawrence JJ

更新日期：1988-03-05 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion. Cys11, His185, Glu222 and Asp233 are the other metal ligands. Wild...

journal_title：Journal of molecular biology

authors： Wang J,Duewel HS,Stuckey JA,Woodard RW,Gatti DL

更新日期：2002-11-22 00:00:00

abstract：:The preparation of hybrid histone octamers with wheat histone H2A variants replacing chicken H2A in the chicken octamer is described. The fidelity of the reconstituted hybrid octamers was confirmed by dimethyl suberimidate cross-linking. Polyglutamic-acid-mediated assembly of these octamers on long DNA and subsequent ...

journal_title：Journal of molecular biology

authors： Lindsey GG,Orgeig S,Thompson P,Davies N,Maeder DL

更新日期：1991-04-20 00:00:00

abstract：:Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured Kd for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)2SO4. These cr...

journal_title：Journal of molecular biology

authors： Fedorov AA,Pollard TD,Almo SC

更新日期：1994-08-19 00:00:00

abstract：:DegP, a member of the highly conserved HtrA family of proteases, performs a regulated proteolysis of toxic misfolded proteins in the periplasm of Gram-negative bacteria. The allosteric switch between inactive and active conformations is a central mechanism to carefully control proteolytic activity of DegP and to maint...

journal_title：Journal of molecular biology

authors： Park H,Kim YT,Choi C,Kim S

更新日期：2017-10-13 00:00:00

abstract：:Proteomics research has delivered many novel tumor targets. However, due to key limitations, it does not specifically identify targets that are most accessible for drug delivery, such as cell-surface antigens. A novel tumor antigen discovery platform based on screening a single domain antibody (sdAb) library against t...

journal_title：Journal of molecular biology

authors： Zhang J,Li Q,Nguyen TD,Tremblay TL,Stone E,To R,Kelly J,Roger MacKenzie C

更新日期：2004-07-30 00:00:00

abstract：:Four-helix bundles are identified and characterized in the subunit interfaces of protein multimers. We find that this motif occurs as often in the interfaces as in the protein monomers. Common and different characteristics demonstrated by the bundles in the two environments suggest the possible stabilization mechanism...

journal_title：Journal of molecular biology

authors： Lin SL,Tsai CJ,Nussinov R

更新日期：1995-04-21 00:00:00

abstract：:In phylogenetic reconstructions by the parsimony method, utilizing 62 sequenced globin genes and pseudogenes (including 34 of the beta-globin gene family from eutherian orders Primates, Lagomorpha, Artiodactyla and Rodentia), the branch of primate psi beta pseudogenes and the goat embryonically expressed epsilon II ge...

journal_title：Journal of molecular biology

authors： Goodman M,Koop BF,Czelusniak J,Weiss ML

更新日期：1984-12-25 00:00:00

abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

journal_title：Journal of molecular biology

authors： Birkeland NK,Lindquist BH

更新日期：1986-04-05 00:00:00

abstract：:The expression of the gene thrS encoding threonyl-tRNA synthetase is under the control of two apparently different regulatory loops: translational feedback regulation and growth rate-dependent control. The translational feedback regulation is due to the binding of threonyl-tRNA synthetase to a site located in the lead...

journal_title：Journal of molecular biology

authors： Comer MM,Dondon J,Graffe M,Yarchuk O,Springer M

更新日期：1996-08-16 00:00:00

abstract：:HtrA (high-temperature requirement A) family proteins play important roles in protein-quality control processes in the bacterial periplasm. A common feature of all members of this family is their modular organization comprising a chymotrypsin-like protease domain and at least one PDZ (postsynaptic density of 95 kDa, d...

journal_title：Journal of molecular biology

authors： Schubert A,Wrase R,Hilgenfeld R,Hansen G

更新日期：2015-08-28 00:00:00

abstract：:To identify structural features in a G-protein-coupled receptor (GPCR) crucial for biosynthesis, stability in the membrane and stability in detergent micelles, we developed an evolutionary approach using expression in the inner membrane of Escherichia coli. From the analysis of 800,000 sequences of the rat neurotensin...

journal_title：Journal of molecular biology

authors： Schlinkmann KM,Hillenbrand M,Rittner A,Künz M,Strohner R,Plückthun A

更新日期：2012-09-21 00:00:00

abstract：:Leech carboxypeptidase inhibitor (LCI) is a 67-residue, tight-binding metallocarboxypeptidase inhibitor composed of a compact domain with a five-stranded beta-sheet and a short alpha-helix that are strongly stabilized by four disulfide bonds. In this study, we investigated the contribution of each particular disulfide...

journal_title：Journal of molecular biology

authors： Arolas JL,Castillo V,Bronsoms S,Aviles FX,Ventura S

更新日期：2009-09-18 00:00:00

abstract：:The crystal structure of desulforedoxin from Desulfovibrio gigas, a new homo-dimeric (2 x 36 amino acids) non-heme iron protein, has been solved by the SIRAS method using the indium-substituted protein as the single derivative. The structure was refined to a crystallographic R-factor of 16.9% at 1.8 A resolution. Nati...

journal_title：Journal of molecular biology

authors： Archer M,Huber R,Tavares P,Moura I,Moura JJ,Carrondo MA,Sieker LC,LeGall J,Romão MJ

更新日期：1995-09-01 00:00:00

abstract：:Conformational fluctuation plays a key role in protein function, but we know little about the associated structural changes. Here we present a general method for elucidating, at the atomic level, a large-scale shape change of a protein molecule in solution undergoing conformational fluctuation. The method utilizes the...

journal_title：Journal of molecular biology

authors： Kitahara R,Yokoyama S,Akasaka K

更新日期：2005-03-25 00:00:00

abstract：:The extracellular matrix in tissues such as bone, tendon and cornea contains ordered, parallel arrays of collagen type I fibrils. Cells embedded in these matrices frequently co-align with the collagen fibrils, suggesting that ordered fibrils provide structural or signalling cues for cell polarization. To study mechani...

journal_title：Journal of molecular biology

authors： Friedrichs J,Taubenberger A,Franz CM,Muller DJ

更新日期：2007-09-21 00:00:00

abstract：:Protein-protein interactions within the membrane, partially or fully mediated by transmembrane (TM) domains, are involved in many vital cellular processes. Since the unique feature of the membrane environment enables protein-protein assembly that otherwise is not energetically favored in solution, the structural restr...

journal_title：Journal of molecular biology

authors： Sal-Man N,Gerber D,Shai Y

更新日期：2004-11-26 00:00:00

abstract：:The effect of divalent cations on the self-association of high molecular weight subfragment-2 (long S-2) and low molecular weight subfragment-2 (short S-2) of rabbit skeletal muscle myosin has been investigated. In the presence of millimolar concentrations of Ca2+ or Mg2+ long S-2 associates at neutral pH to form orde...

journal_title：Journal of molecular biology

authors： Ueno H,Rodgers ME,Harrington WF

更新日期：1983-08-05 00:00:00

abstract：:Pax5 (paired box binding factor 5) is a critical regulator of transcription and lineage commitment in B lymphocytes. In B cells, mb-1 (Ig-alpha/immunoglobulin-associated alpha) promoter transcription is activated by Pax5 through its recruitment of E74-like transforming sequence (Ets) family proteins to a composite sit...

journal_title：Journal of molecular biology

authors： Fitzsimmons D,Lukin K,Lutz R,Garvie CW,Wolberger C,Hagman J

更新日期：2009-09-18 00:00:00

abstract：:Binding of the histone (H2A, H2B) dimer with chicken erythrocyte DNA has been studied by salt-titration spectroscopy in equilibrium conditions. The circular dichroism of DNA near 275 nm is depressed by the interaction with (H2A, H2B) at low concentrations of salt. The depression increases with increasing amounts of (H...

journal_title：Journal of molecular biology

authors： Oohara I,Wada A

更新日期：1987-07-20 00:00:00

abstract：:A method using binding site "neighbor-effect" parameters (NEPs) is introduced to evaluate the effects of interaction between adjacent ligands on their binding to an infinite linear lattice. Binding site overlap is also taken into account. This enables the conditional probability approach of McGhee & von Hippel to be e...

journal_title：Journal of molecular biology

authors： Wolfe AR,Meehan T

更新日期：1992-02-20 00:00:00

abstract：:The capsid (CA) protein plays crucial roles in HIV infection and replication, essential to viral maturation. The absence of high-resolution structural data on unassembled CA hinders the development of antivirals effective in inhibiting assembly. Unlike enzymes that have targetable, functional substrate-binding sites, ...

journal_title：Journal of molecular biology

authors： Du S,Betts L,Yang R,Shi H,Concel J,Ahn J,Aiken C,Zhang P,Yeh JI

更新日期：2011-02-25 00:00:00

abstract：:A single mutation in the wild-type transthyretin (WT TTR) such as V30M causes a familial amyloidotic polyneuropathy disease. Comparison of the three-dimensional crystal structures of WT and V30M does not tell much about the reason. High-pressure NMR revealed that at neutral pH both WT and V30M exist as equilibrium bet...

journal_title：Journal of molecular biology

authors： Niraula TN,Haraoka K,Ando Y,Li H,Yamada H,Akasaka K

更新日期：2002-07-05 00:00:00

abstract：:Fish hatching enzymes are zinc metalloproteases that digest the egg envelope (chorion) at the time of hatching. The crystal structure of zebrafish hatching enzyme 1 (ZHE1) has been solved at 1.10 Å resolution. ZHE1 is monomeric, is mitten shaped, and has a cleft at the center of the molecule. ZHE1 consists of three 3(...

journal_title：Journal of molecular biology

authors： Okada A,Sano K,Nagata K,Yasumasu S,Ohtsuka J,Yamamura A,Kubota K,Iuchi I,Tanokura M

更新日期：2010-10-08 00:00:00

abstract：:Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacteri...

journal_title：Journal of molecular biology

authors： Zhang L,Wang Y,Picking WL,Picking WD,De Guzman RN

更新日期：2006-06-02 00:00:00