Abstract:
RATIONALE AND OBJECTIVES:The aims of this study were to label the versatile amino acid l-lysine with (99m)Tc using 2,3-dimercapto-succinic acid (DMSA) as a chelator, and to assess its tumor imaging feasibility under in vivo and in vitro conditions, and finally to determine the subcellular biodistribution of this radiopharmaceutical. MATERIALS AND METHODS:DMSA-l-lysine was chemically synthesized and labeled with sodium pertechnetate. Nuclear magnetic resonance (NMR) and mass spectral analysis of DMSA-l-lysine were conducted. Radiochemical purity was determined by thin-layer chromatography (TLC) and paper chromatography. Cellular uptake, competition and subcellular localization studies were performed in rat breast cancer cells (13762). In vivo studies of planar imaging and biodistribution studies were performed on female Fischer 344 rats. Medical Internal Radiation Dose (MIRD) dosimetry estimates were calculated. RESULTS:Radiochemical purity (determined by radio-TLC and high-performance liquid chromatography) of these compounds was >95%. (99m)Tc-DMSA-l-lysine showed good uptake in in vitro cell culture assays and uptake was reduced in competition studies. (99m)Tc-DMSA-l-lysine accumulates in the nucleus as much as in the cytoplasm and it was also shown that accumulation of the (99m)Tc-DMSA-l-lysine in the nucleus increases as a function of a time. There was an increase in tumor-to-blood and tumor-to-muscle count density ratios. Tumor/background ratios were 5.75 at 1 hour and 6.87 at 2 hours. In vivo tissue distribution studies revealed that radiation dosimetry of blood-forming organs were within radiation dose limits. CONCLUSION:DMSA-l-lysine kits can be labeled with (99m)Tc easily and efficiently, with high radiochemical purity and cost-effectiveness. In vitro cellular uptake and scintigraphic imaging studies demonstrated the pharmacokinetic distribution and feasibility of using (99m)Tc-DMSA-l-lysine for tumor imaging.
journal_name
Acad Radioljournal_title
Academic radiologyauthors
Karacalioglu AO,Yang DJ,Azhdarinia A,Mendez R,Oh C,Kohanim S,Chanda M,Greenwell AC,Yu DF,Kim EEdoi
10.1016/j.acra.2006.03.022subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1327-37issue
11eissn
1076-6332issn
1878-4046pii
S1076-6332(06)00417-Xjournal_volume
13pub_type
杂志文章abstract:RATIONALE AND OBJECTIVES:The goal of this article is to analyze and compare the performance of a developed mass computer-aided detection (CAD) system that takes breast density information into account when using manual or automatic breast density annotations in the training step. The advantages of considering this brea...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2010.04.013
更新日期:2010-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To investigate the performance of diffusion kurtosis imaging (DKI) and diffusion tensor imaging (DTI) in discriminating benign tissue, low- and high-grade prostate adenocarcinoma (PCa). MATERIALS AND METHODS:Forty-eight patients with biopsy-proven PCa of different Gleason grade (GG), who provi...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2018.11.015
更新日期:2019-10-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The purpose of this prospective randomized study was to compare the long-term instructional effectiveness of a computer-based radiology multimedia textbook (MMTB) with that of a traditional lecture. METHODS:Volunteer faculty/fellows and residents were randomly assigned to either a computer-bas...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(05)80731-7
更新日期:1994-11-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:A novel ventilation imaging method based on four-dimensional (4D) computed tomography (CT) has been applied to the field of radiation oncology. Understanding its reproducibility is a prerequisite for clinical applications. The purpose of this study was to quantify the reproducibility of 4D CT v...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2012.07.006
更新日期:2012-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:We investigated the influence of muscular activity and regional body temperature changes on the accumulation of intravenously (i.v.) administered, dextran-coated superparamagnetic iron oxide (SPIO) particles in the lymph nodes of rats. METHODS:Four groups of rats (N = 21) were used. Five rats ...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(96)80193-0
更新日期:1996-08-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:This study aimed to measure apparent diffusion coefficient (ADC) in Chinese patients with newly diagnosed multiple myeloma by whole-body diffusion-weighted magnetic resonance imaging (WB-DWI MRI) and assess the diagnostic accuracy of ADC in the discrimination of deep response to induction chemo...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2017.12.008
更新日期:2018-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The Liver Imaging Reporting and Data System (LI-RADS) is a newly developed nomogram for standardizing the performance and interpretation of liver imaging. However, it is unclear which imaging technique is optimal to exactly define LI-RADS scale. This study aims to determine the concordance of c...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2015.11.002
更新日期:2016-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To identify the presence and extent of artifacts in prostate diffusion-weighted magnetic resonance imaging (DW-MRI) and discuss tradeoffs between imaging at 1.5 T (1.5 T) and 3.0 T (3.0 T). In addition, we aim to provide quantitative estimates of signal-to-noise ratios (SNRs) at both field stre...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2013.04.005
更新日期:2013-08-01 00:00:00
abstract::Innovation in the field of diagnostic imaging is based primarily on the availability of new and improved equipment that opens the door for new clinical applications. Payments for these imaging procedures are subject to complex Medicare price control schemes, affecting incentives for appropriate use and innovation. Ach...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2011.04.007
更新日期:2011-09-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Renovascular smooth muscle contractility, an important factor in contrast media-induced nephrotoxicity, depends on intracellular Ca2+ concentration, which is composed of extracellular Ca2+ influx and intracellular Ca2+ release. These factors were investigated in contrast media-induced renal vas...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(96)80298-4
更新日期:1996-11-01 00:00:00
abstract::The purpose of this work is to provide current information on the rapidly evolving subject of nephrogenic systemic fibrosis (NSF), to establish the radiologic approach to the management of high-risk patients for NSF, and to assess the probabilistic risk of NSF compared to contrast induced nephropathy (CIN), as encount...
journal_title:Academic radiology
pub_type: 杂志文章,评审
doi:10.1016/j.acra.2009.01.001
更新日期:2009-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Renal tubular vacuolization (RTV), which has been shown to occur after the use of iodinated contrast material, may be one of the earliest signs of contrast medium-induced renal injury. In this study, the authors tested a method for preventing RTV with the administration of acetylcysteine, theop...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(03)80061-2
更新日期:2003-05-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the ex vivo ablation zones created in hepatic tissue using monopolar and bipolar gas- and water-cooled radiofrequency (RF) applicators. MATERIALS AND METHODS:RF ablations were performed on ex vivo bovine liver tissue using closed circuit water-cooled and closed circuit cryogenicall...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2014.02.004
更新日期:2014-05-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate cone beam computed tomography (CBCT) for monitoring of tibial bone defect healing in comparison to histopathological findings. MATERIALS AND METHODS:Circumscribed tibial bone defects were created in 16 mini-pigs and imaging of the tibia was performed on day 42 using a modern CBCT s...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2011.10.022
更新日期:2012-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Because several factors are involved in cancer detection, a malignant lesion that is visible on a mammogram will not necessarily be reported by the radiologist reading the case. Indeed, a significant fraction of screening-detected cancers are visible in retrospect, and were perceived by the rad...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2004.04.015
更新日期:2004-08-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to develop a computer-aided diagnosis (CAD) system in assessing the sonographic morphologic and texture features of soft-tissue tumors. MATERIALS AND METHODS:The retrospective study involved 114 pathology proven cases including 73 benign and 41 malignant soft-tissue t...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2009.07.024
更新日期:2009-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Diffraction-enhanced imaging (DEI) is a type of phase contrast x-ray imaging that has improved image contrast at a lower dose than conventional radiography for many imaging applications, but no studies have been done to determine if DEI might be useful for diagnosing lung injury. The goals of t...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2011.08.005
更新日期:2011-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Mammographic density and parenchymal patterns have been shown to be related to the risk of developing breast cancer. Thus, computerized texture analysis of breast parenchymal patterns on mammograms may be useful in assessing breast cancer risk. MATERIALS AND METHODS:A comparative evaluation wa...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2005.03.069
更新日期:2005-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVE:Today, the exchange of medical images and clinical information is well defined by the digital imaging and communications in medicine (DICOM) and Health Level Seven (ie, HL7) standards. The interoperability among information systems is specified by the integration profiles of IHE (Integrating the...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2004.11.015
更新日期:2005-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the ability of dynamic contrast-enhanced magnetic resonance imaging (MRI) to differentiate several tumor entities of the parotid gland in a prospective clinical trial. MATERIALS AND METHODS:A total of 112 patients with parotid tumors were examined with dynamic contrast-enhanced 1.5...
journal_title:Academic radiology
pub_type: 临床试验,杂志文章
doi:10.1016/j.acra.2007.03.004
更新日期:2007-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the reproducibility of Modified Response Evaluation Criteria in Solid Tumors (mRECIST) for hepatocellular carcinoma (HCC) after transarterial chemoembolization (TACE) using multiphasic computed tomography. MATERIALS AND METHODS:The institutional review board approved this retrospec...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2018.02.013
更新日期:2018-11-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The purpose of this study is to quantify the clinical impact of resident-attending discrepancies at a tertiary referral academic radiology residency program by assessing rates of intervention, discrepancy confirmation, recall rate, and management change rate; furthermore, a discrepancy categori...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2017.11.016
更新日期:2018-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The authors evaluated the diagnostic accuracy of viewing computed tomographic (CT) scans as film versus soft-copy images at a workstation. METHODS:Receiver operating characteristic analysis of the interpretation of 202 CT scans (103 were normal, 99 were abnormal) by five neuroradiologists was ...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(97)80004-9
更新日期:1997-02-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To estimate the gain in signal-to-noise ratio (SNR) in first-pass contrast-enhanced (CE) abdominal magnetic resonance angiography (MRA) at 3.0 T compared with 1.5 T. MATERIALS AND METHODS:Three protocols were simulated using six contrast agents: gadopentetate dimeglumine (Magnevist, Berlex, Wa...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2007.03.007
更新日期:2007-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To determine computed tomography (CT) radiological features and texture features that are rewarding in differentiating nonhypervascular pancreatic neuroendocrine neoplasms (PNENs) from pancreatic ductal adenocarcinomas (PDACs). MATERIALS AND METHODS:We compared patients to pathologically prove...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2019.06.012
更新日期:2020-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To compare the results of two software-based methods, Quantra and Volpara, for volumetric breast composition assessment. MATERIALS AND METHODS:Four hundred forty-five normal, bilateral, two-view, digital mammograms were included. Breast volume (BV), fibroglandular tissue volume (FTV), and perc...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2014.12.003
更新日期:2015-04-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:A number of studies have reported decreases in fractional anistropy (FA) in amyotrophic lateral sclerosis using diffusion tensor imaging (DTI). The purpose of this study was to perform a meta-analysis in order to estimate the diagnostic test accuracy measures of DTI for the diagnosis of amyotro...
journal_title:Academic radiology
pub_type: 杂志文章,meta分析
doi:10.1016/j.acra.2012.04.012
更新日期:2012-09-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this report is to investigate the magnetic field dependence of the signal-to-noise ratio (SNR) for carotid vessel wall magnetic resonance imaging using phased-array (PA) surface coils by comparing images obtained at 1.5 and 3 Tesla (T) and determine the extent to which the improved S...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2005.08.009
更新日期:2005-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To analyze the statistical biases in the studies used to derive cardiac magnetic resonance-based major and minor criteria for the diagnosis of arrhythmogenic right ventricular cardiomyopathy (ARVC). MATERIALS AND METHODS:ARVC is a rare disorder of the heart that can lead to sudden death in you...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2015.04.009
更新日期:2015-08-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To investigate the value of texture analysis and conventional magnetic resonance imaging (MRI) features for predicting the early recurrence (ER) of single hepatocellular carcinoma (HCC) after hepatectomy. MATERIALS AND METHODS:A total of 100 HCC patients were first divided into group A (tumor ...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2018.10.011
更新日期:2019-09-01 00:00:00