Abstract：
OBJECTIVE:To assess the effectiveness of in vivo gene therapy to treat subcutaneous tumors generated from murine lung cancer cells. MATERIAL AND METHODS:C57BL/6 mice received subcutaneus injections of 5 x 105 cells from the murine Lewis lung cancer cell line. By 10 days, subcutaneous tumors of approximately 5 mm diameter were formed. At that point, treatment was provided by intratumor injection of a replication-defective recombinant adenovirus carrying the gene for thymidine kinase (AdCMV-Tk) or interleukin (IL) 12 (AdCMV-IL12), or by injection of syngeneic dendritic cells previously transduced with adenovirus containing the IL-12 gene (DC-IL12). Control groups were treated with saline or adenovirus containing the gene for beta-galactosidase (AdCMV-LacZ), which functions as a reporter gene and does not have a therapeutic effect. The number of animals in each group ranged from 14 to 25 in experiments using adenovirus and from 10 to 12 in experiments using dendritic cells. Tumor size was followed for 3 weeks in the case of treatment with adenovirus and 4 weeks for treatment with dendritic cells. RESULTS:A significant reduction in subcutaneous tumor growth was observed in the groups treated with AdCMV-Tk, AdCMV-IL12, and DC-IL12 compared with control groups treated with saline or AdCMV-LacZ. The difference was statistically significant from day 7 of treatment in the AdCMV-Tk group, from day 9 in the AdCMV-IL12 group, and from day 10 in the DC-IL12 group, and in all cases it was maintained until the end of the follow-up period. CONCLUSIONS:Gene therapy with AdCMV-Tk, AdCMV-IL12, or DC-IL12 is effective in our model of subcutaneous tumors arising from cells of the Lewis lung cancer cell line. The treatment leads to a significant reduction in tumor growth compared with control groups.
journal_name
Arch Bronconeumoljournal_title
Archivos de bronconeumologiaauthors
Rodrigo Garzón M,Tirapu Fernández de la Cuesta I,Arina Iraeta A,Centelles Llorente MN,Zulueta Francés Jsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
526-32issue
10eissn
0300-2896issn
1579-2129pii
13093396journal_volume
42pub_type
杂志文章abstract：INTRODUCTION:Community-acquired pneumonia (CAP) is a common serious infection. This study aimed to evaluate the prognostic utility of neutrophil count percentage (NCP) and neutrophil-lymphocyte ratio (NLR) in patients with CAP. METHODS:Retrospective study of hospitalized patients with CAP. Patients had a blood test at...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2019.02.005
更新日期：2019-09-01 00:00:00
abstract：:Nocardia is a gram-positive bacillus that infects mainly immunodepressed patients. Its association with lupus erythematosus has been described only occasionally and we have found no reports in the literature of an association between lupus and acute respiratory distress syndrome due to pulmonary nocardiosis. We presen...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s1579-2129(06)60223-1
更新日期：2005-05-01 00:00:00
abstract：:During the natural history of patients with chronic obstructive pulmonary disease (COPD), an increase in symptom severity can occur, called exacerbated COPD (ECOPD), sometimes requiring hospital admission. Although there is ongoing debate on the definition of ECOPD--leading to a lack of homogeneity in the numerous stu...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,评审
doi：10.1016/S0300-2896(10)70061-2
更新日期：2010-01-01 00:00:00
abstract：INTRODUCTION:Intermediate respiratory care units (IRCU) provide continuous monitoring and non-invasive mechanical ventilation (NIMV) in patients with severe respiratory failure who are usually admitted to intensive care units (ICU). The usefulness of IRCU in managing severe asthma exacerbations has never been evaluated...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2012.11.009
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:To analyse the results obtained in the diagnosis and staging of lung cancer (LC) by a Lung Cancer Rapid Diagnosis Unit (LC-RDU) in which real-time endobronchial ultrasound-guided transbronchial needle aspiration (RT-EBUS guided-TBNA) is performed as part of the clinical evaluation of the patient prior to trea...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2010.07.005
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the diagnostic efficacy of pleural procedures, safety, delay and cost of the diagnosis of pleural effusion (PE) by analysing the parameters that are dependent on the area of patient management (outpatient or inpatient). PATIENTS AND METHODS:Prospective non-randomized study. Two groups were establ...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2010.05.010
更新日期：2010-09-01 00:00:00
abstract：:Despite the administration of appropriate treatment, a high number of patients with asthma remain uncontrolled. This suggests the need for alternative treatments that are effective, safe and selective for the established asthma phenotypes, especially in patients with uncontrolled severe asthma. The most promising opti...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,评审
doi：10.1016/j.arbres.2014.02.002
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:The current goal of asthma treatment is to achieve and maintain control. This study aimed to explore the relationship between the ACT (Asthma Control Test) questionnaire and the levels of control according to GINA (Global Initiative for Asthma) to establish the cut-off points for the ACT and evaluate its r...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,多中心研究
doi：10.1016/j.arbres.2010.04.003
更新日期：2010-07-01 00:00:00
abstract：INTRODUCTION:COPD and emphysema due to alpha-1 antitrypsin deficiency (AATD) are the first and fourth indications for lung transplantation worldwide, respectively. Despite this, there is little information regarding the health status of these patients at the time of transplantation. METHODS:Patients who received a lun...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2014.08.003
更新日期：2015-08-01 00:00:00
abstract：:We report our 3-years experience with video-thoracoscopy for pneumothorax. Forty-six endoscopies (34 men, 12 women; mean age 27 +/- 9 years) were performed. Patients were under general anesthesia and intubation was selective. The conventional approach was used, with 3 incisions through which the camera and endoscopic ...
journal_title：Archivos de bronconeumologia
pub_type： 临床试验,杂志文章
doi：10.1016/s0300-2896(15)30814-0
更新日期：1996-02-01 00:00:00
abstract：BACKGROUND:Results supporting the use and the effectiveness of positive expiratory, pressure devices in chronic obstructive pulmonary disease (COPD) patients are still controversial, We have tested the hypothesis that adding TPEP or IPPB to standard pharmacological therapy may provide additional clinical benefit over, ...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.arbres.2013.07.019
更新日期：2014-01-01 00:00:00
abstract：:Thirty-one patients with pulmonary aspergilloma were studied retrospectively, leading to the following main findings: 1) Men are most often affected (90% of cases). 2) Diagnosis occurs significantly more often when the patient is between 50 to 60 years of age. 3) Aspergilloma originates most often in residual pulmonar...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s0300-2896(15)31014-0
更新日期：1994-11-01 00:00:00
abstract：INTRODUCTION AND OBJECTIVE:Non-tuberculous mycobacteria (NTM) isolates are becoming more common. The main objective of our study was to establish the number and diversity of NTM species in our region and their distribution according to the source sample, age and gender of the patients, and to analyse clinically signifi...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2017.02.014
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:To analyze the network structure of collaboration between medical centers sharing authorship of scientific articles on smoking. MATERIAL AND METHODS:Articles reporting smoking research by authors from 2 or more Spanish medical centers between 1999 and the end of 2003 were identified through the Science Citat...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,评审
doi：10.1016/s1579-2129(07)60089-5
更新日期：2007-07-01 00:00:00
abstract：:Asthma continues to be a global health problem, despite advances in diagnostic techniques and treatment. The inflammatory nature of asthma is currently indisputable, as is the involvement of the entire respiratory tree, both the proximal and most distal airways, which has been demonstrated in multiple studies. The dev...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,评审
doi：10.1016/S0300-2896(11)70016-3
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:As the research undertaken to date on the efficacy of the new antibiotics in the treatment of exacerbations of chronic bronchitis has taken the form of trials designed to demonstrate equivalence, we have no data on the advantages associated with the use of these new drugs with greater bactericidal activity. O...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,meta分析,评审
doi：10.1016/s1579-2129(07)60016-0
更新日期：2007-01-01 00:00:00
abstract：INTRODUCTION:Persistent air leak is frustrating for both patients and physicians, above all leaks with a high risk of surgery. Insertion of endobronchial valves could be an alternative to surgery. The aim of this study is to describe our experience in these valves and analyse their efficacy in a series of patients with...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2014.04.007
更新日期：2015-01-01 00:00:00
abstract：:Conventional polysomnography (CPS) is a complex, costly procedure that is not widely available, meaning that it is difficult to apply it in patients suspected of having obstructive sleep apnea syndrome (OSAS). Various procedures have therefore been proposed for screening candidates for CPS. We studied the usefulness o...
journal_title：Archivos de bronconeumologia
pub_type： 临床试验,杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:In the last few years, considerable interest has been aroused in the treatment of chronic obstructive pulmonary disease (COPD) and the dearth of therapeutic options has been replaced by a far more proactive treatment approach, reflected in the development of the various guidelines and recommendations of the scientific...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,评审
doi：10.1016/S0300-2896(10)70050-8
更新日期：2010-12-01 00:00:00
abstract：:Ten cases of pulmonary mycetoma diagnosed in our service between 1985-1992 were retrospectively studied. Clinical methodology followed in the study is commented and the different therapeutic options are reviewed, so the literature concerning this process. The unspecificity of serum precipitins with Aspergillus is emph...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s0300-2896(15)31048-6
更新日期：1994-08-01 00:00:00
abstract：:Pulmonary neoplasms can deposit malignant cells in the pleural cavity by a variety of mechanisms, depending not only on histological type but also on clinical stage. We investigated the effect on survival rate of a finding of malignant cells in pleural lavage. We also investigated the effect of the presence of pleural...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：OBJECTIVE:To analyze the factors that determine the risk of morbidity and mortality associated with lung resection in patients with bronchogenic carcinoma. PATIENTS AND METHODS:Prospective multicenter study conducted between October 1, 1993 and September 30, 1997 in the 19 hospitals that make up the Bronchogenic Carci...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s1579-2129(07)60038-x
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVE:Mortality due to lung cancer in Spain is increasing continuously. The aim of the present study was to collect information on the hospital incidence of lung cancer, as well as information on clinical management, in different regions of Spain. MATERIAL AND METHODS:A prospective observational study of patients ...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,多中心研究
doi：
更新日期：2006-09-01 00:00:00
abstract：INTRODUCTION:The effectiveness of needle aspiration in the initial treatment of primary spontaneous pneumothorax has been widely studied. The objective of this research was to compare digital with manual aspiration in a randomized clinical trial. METHODS:We designed a blinded parallel-group randomized clinical trial w...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2020.01.016
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVE:To describe our experience in managing patients with Duchenne muscular dystrophy. PATIENTS AND METHODS:We analyzed the following variables in a group of 27 patients with Duchenne muscular dystrophy: arterial blood gases, lung function before and after mechanical ventilation, oxygen saturation (measured by pu...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s1579-2129(07)60127-x
更新日期：2007-10-01 00:00:00
abstract：OBJECTIVE:In the studies carried out to date, the cost of chronic obstructive pulmonary disease (COPD) may have been overestimated due to the inclusion of previously diagnosed patients seeking medical attention for their symptoms. As a result, the severity of the cases included in these studies may have been greater th...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s1579-2129(06)60198-5
更新日期：2004-02-01 00:00:00
abstract：INTRODUCTION:The purpose of the present study is to analyse the prevalence and distribution of asbestos lung residue in the Barcelona urban population. MATERIAL AND METHODS:Lung autopsy samples were obtained from 35 individuals who had lived in Barcelona. The close family were interviewed in order to rule out asbestos...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2009.12.008
更新日期：2010-04-01 00:00:00
abstract：OBJECTIVE:Diffuse interstitial lung diseases (DILD) form a group of diseases which affect the alveolar interstitial space and share very similar clinical, radiological, and functional features, making lung biopsy essential for establishing diagnosis, prognosis, and treatment in many cases. We aimed to see whether there...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/s1579-2129(06)60284-x
更新日期：2005-10-01 00:00:00
abstract：:Patient cooperation in controlling asthma is a key element for achieving the most efficient therapy possible according to current guidelines. Cooperation requires that the patient be adequately informed about his disease and able to make certain decisions. The aim of this study was to analyze whether patients really d...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章,多中心研究
doi：10.1016/s0300-2896(15)30039-9
更新日期：1999-10-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to verify the reproducibility between two different observers of an analysis method for diaphragmatic displacement measurements using direct visualization with videofluoroscopy. PATIENTS AND METHODS:29 mouth breathing children aged 5 to 12 years from both genders were analyzed. ...
journal_title：Archivos de bronconeumologia
pub_type： 杂志文章
doi：10.1016/j.arbres.2010.12.011
更新日期：2011-06-01 00:00:00
