Ammonium toxicity and potassium limitation in yeast.

abstract：:Commensal microbes, whether they are beneficial or pathogenic, are sensitive to host processes that starve or swamp the prokaryote with large fluctuations in local zinc concentration. To understand how microorganisms coordinate a dynamic response to changes in zinc availability at the molecular level, we evaluated the...

journal_title：PLoS biology

authors： Gilston BA,Wang S,Marcus MD,Canalizo-Hernández MA,Swindell EP,Xue Y,Mondragón A,O'Halloran TV

更新日期：2014-11-04 00:00:00

abstract：:Several strategies have been pursued to increase the extent of exon 7 inclusion during splicing of SMN2 (survival of motor neuron 2) transcripts, for eventual therapeutic use in spinal muscular atrophy (SMA), a genetic neuromuscular disease. Antisense oligonucleotides (ASOs) that target an exon or its flanking splice ...

journal_title：PLoS biology

authors： Hua Y,Vickers TA,Baker BF,Bennett CF,Krainer AR

更新日期：2007-04-01 00:00:00

abstract：:Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have been identified, which are present in most eukaryotes. We report the existence of a fifth AP complex, AP-5. Tagged AP-5 localises to a late endosomal compartment i...

journal_title：PLoS biology

authors： Hirst J,Barlow LD,Francisco GC,Sahlender DA,Seaman MN,Dacks JB,Robinson MS

更新日期：2011-10-01 00:00:00

abstract：:Citizen science is mainstream: millions of people contribute data to a growing array of citizen science projects annually, forming massive datasets that will drive research for years to come. Many citizen science projects implement a "leaderboard" framework, ranking the contributions based on number of records or spec...

journal_title：PLoS biology

authors： Callaghan CT,Rowley JJL,Cornwell WK,Poore AGB,Major RE

更新日期：2019-06-27 00:00:00

abstract：:The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations in human XPD are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. We performed a comparative analysis of XPD f...

journal_title：PLoS biology

authors： Kuper J,Braun C,Elias A,Michels G,Sauer F,Schmitt DR,Poterszman A,Egly JM,Kisker C

更新日期：2014-09-30 00:00:00

abstract：:The anaerobic bacterium Fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of Na⁺. Here, we demonstrate that this ion-motive force is directly coupled to ATP synthesis, via an F₁F₀-ATP synthase with a novel Na⁺ recognition motif, shared by other human pathogens. Molecular model...

journal_title：PLoS biology

authors： Schulz S,Iglesias-Cans M,Krah A,Yildiz O,Leone V,Matthies D,Cook GM,Faraldo-Gómez JD,Meier T

更新日期：2013-01-01 00:00:00

abstract：:Robust biological systems are expected to accumulate cryptic genetic variation that does not affect the system output in standard conditions yet may play an evolutionary role once phenotypically expressed under a strong perturbation. Genetic variation that is cryptic relative to a robust trait may accumulate neutrally...

journal_title：PLoS biology

authors： Duveau F,Félix MA

更新日期：2012-01-01 00:00:00

abstract：:The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were developed to address the lack of reproducibility in biomedical animal studies and improve the communication of research findings. While intended to guide the preparation of peer-reviewed manuscripts, the principles of transparent reporting ...

journal_title：PLoS biology

authors： Karp NA,Meehan TF,Morgan H,Mason JC,Blake A,Kurbatova N,Smedley D,Jacobsen J,Mott RF,Iyer V,Matthews P,Melvin DG,Wells S,Flenniken AM,Masuya H,Wakana S,White JK,Lloyd KC,Reynolds CL,Paylor R,West DB,Svenson KL,

更新日期：2015-05-20 00:00:00

abstract：:Amyloid-β (Aβ) accumulation in the brain is a hallmark of Alzheimer's disease (AD) pathology. However, the molecular mechanism controlling microglial Aβ phagocytosis is poorly understood. Here we found that the E3 ubiquitin ligase Pellino 1 (Peli1) is induced in the microglia of AD-like five familial AD (5×FAD) mice, ...

journal_title：PLoS biology

authors： Xu J,Yu T,Pietronigro EC,Yuan J,Arioli J,Pei Y,Luo X,Ye J,Constantin G,Mao C,Xiao Y

更新日期：2020-10-05 00:00:00

abstract：:Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC) respond to numerous hormonal and neural signals, resulting in changes in food intake. Here, we demonstrate that ARC POMC neurons express capsaicin-sensitive transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors. To s...

journal_title：PLoS biology

authors： Jeong JH,Lee DK,Liu SM,Chua SC Jr,Schwartz GJ,Jo YH

更新日期：2018-04-24 00:00:00

abstract：:Lysosomes are ubiquitous acidified organelles that degrade intracellular and extracellular material trafficked via multiple pathways. Lysosomes also sense cellular nutrient levels to regulate target of rapamycin (TOR) kinase, a signaling enzyme that drives growth and suppresses activity of the MiT/TFE family of transc...

journal_title：PLoS biology

authors： Murphy JT,Liu H,Ma X,Shaver A,Egan BM,Oh C,Boyko A,Mazer T,Ang S,Khopkar R,Javaheri A,Kumar S,Jiang X,Ory D,Mani K,Matkovich SJ,Kornfeld K,Diwan A

更新日期：2019-05-14 00:00:00

abstract：:Recent evidence suggests that many malignancies, including breast cancer, are driven by a cellular subcomponent that displays stem cell-like properties. The protein phosphatase and tensin homolog (PTEN) is inactivated in a wide range of human cancers, an alteration that is associated with a poor prognosis. Because PTE...

journal_title：PLoS biology

authors： Korkaya H,Paulson A,Charafe-Jauffret E,Ginestier C,Brown M,Dutcher J,Clouthier SG,Wicha MS

更新日期：2009-06-02 00:00:00

abstract：:For the last 100 years, it has been uncontroversial to state that the plant germline is set aside late in development, but there is surprisingly little evidence to support this view. In contrast, much evolutionary theory and several recent empirical studies seem to suggest the opposite-that the germlines of some and p...

journal_title：PLoS biology

authors： Lanfear R

更新日期：2018-05-16 00:00:00

abstract：:Fusion proteins composed of the histone methyltransferase mixed-lineage leukemia (MLL) and a variety of unrelated fusion partners are highly leukemogenic. Despite their prevalence, particularly in pediatric acute leukemia, many molecular details of their transforming mechanism are unknown. Here, we provide mechanistic...

journal_title：PLoS biology

authors： Mueller D,García-Cuéllar MP,Bach C,Buhl S,Maethner E,Slany RK

更新日期：2009-11-01 00:00:00

abstract：:Certain bacterial adhesins appear to promote a pathogen's extracellular lifestyle rather than its entry into host cells. However, little is known about the stimuli elicited upon such pathogen host-cell interactions. Here, we report that type IV pili (Tfp)-producing Neisseria gonorrhoeae (P(+)GC) induces an immediate r...

journal_title：PLoS biology

authors： Boettcher JP,Kirchner M,Churin Y,Kaushansky A,Pompaiah M,Thorn H,Brinkmann V,Macbeath G,Meyer TF

更新日期：2010-08-24 00:00:00

abstract：:Science communication and outreach are essential for training the next generation of scientists and raising public awareness for science. Providing effective science, technology, engineering, and mathematics (STEM) educational outreach to students in classrooms is challenging because of the need to form partnerships w...

journal_title：PLoS biology

authors： Kompella P,Gracia B,LeBlanc L,Engelman S,Kulkarni C,Desai N,June V,March S,Pattengale S,Rodriguez-Rivera G,Ryu SW,Strohkendl I,Mandke P,Clark G

更新日期：2020-03-30 00:00:00

abstract：:Members of the highly conserved class of BEACH domain containing proteins (BDCPs) have been established as broad facilitators of protein-protein interactions and membrane dynamics in the context of human diseases like albinism, bleeding diathesis, impaired cellular immunity, cancer predisposition, and neurological dys...

journal_title：PLoS biology

authors： Steffens A,Bräutigam A,Jakoby M,Hülskamp M

更新日期：2015-07-02 00:00:00

abstract：:Planarians display remarkable plasticity in maintenance of their germline, with the ability to develop or dismantle reproductive tissues in response to systemic and environmental cues. Here, we investigated the role of G protein-coupled receptors (GPCRs) in this dynamic germline regulation. By genome-enabled receptor ...

journal_title：PLoS biology

authors： Saberi A,Jamal A,Beets I,Schoofs L,Newmark PA

更新日期：2016-05-10 00:00:00

abstract：:The transcription rate of a gene is often controlled by several regulators that bind specific sites in the gene's cis-regulatory region. The combined effect of these regulators is described by a cis-regulatory input function. What determines the form of an input function, and how variable is it with respect to mutatio...

journal_title：PLoS biology

authors： Mayo AE,Setty Y,Shavit S,Zaslaver A,Alon U

更新日期：2006-04-01 00:00:00

abstract：:The mechanisms that regulate how dendrites target different neurons to establish connections with specific cell types remain largely unknown. In particular, the formation of cell-type-specific connectivity during postnatal neurogenesis could be either determined by the local environment of the mature neuronal circuit ...

journal_title：PLoS biology

authors： Kelsch W,Mosley CP,Lin CW,Lois C

更新日期：2007-11-01 00:00:00

abstract：:During pregnancy, the ETS transcription factor ELF5 establishes the milk-secreting alveolar cell lineage by driving a cell fate decision of the mammary luminal progenitor cell. In breast cancer, ELF5 is a key transcriptional determinant of tumor subtype and has been implicated in the development of insensitivity to an...

journal_title：PLoS biology

authors： Gallego-Ortega D,Ledger A,Roden DL,Law AM,Magenau A,Kikhtyak Z,Cho C,Allerdice SL,Lee HJ,Valdes-Mora F,Herrmann D,Salomon R,Young AI,Lee BY,Sergio CM,Kaplan W,Piggin C,Conway JR,Rabinovich B,Millar EK,Oakes SR,C

更新日期：2015-12-30 00:00:00

abstract：:Chemotaxis allows bacteria to colonize their environment more efficiently and to find optimal growth conditions, and is consequently under strong evolutionary selection. Theoretical and experimental analyses of bacterial chemotaxis suggested that the pathway has been evolutionarily optimized to produce robust output u...

journal_title：PLoS biology

authors： Løvdok L,Bentele K,Vladimirov N,Müller A,Pop FS,Lebiedz D,Kollmann M,Sourjik V

更新日期：2009-08-01 00:00:00

abstract：:Over the past decade, biology has undergone a data revolution in how researchers collect data and the amount of data being collected. An emerging challenge that has received limited attention in biology is managing, working with, and providing access to data under continual active collection. Regularly updated data pr...

journal_title：PLoS biology

authors： Yenni GM,Christensen EM,Bledsoe EK,Supp SR,Diaz RM,White EP,Ernest SKM

更新日期：2019-01-29 00:00:00

abstract：:While the role of cortical microstructure in organising neural function is well established, it remains unclear how structural constraints can give rise to more flexible elements of cognition. While nonhuman primate research has demonstrated a close structure-function correspondence, the relationship between microstru...

journal_title：PLoS biology

authors： Paquola C,Vos De Wael R,Wagstyl K,Bethlehem RAI,Hong SJ,Seidlitz J,Bullmore ET,Evans AC,Misic B,Margulies DS,Smallwood J,Bernhardt BC

更新日期：2019-05-20 00:00:00

abstract：:Three-dimensional (3D) structural information on many length scales is of central importance in biological research. Excellent methods exist to obtain structures of molecules at atomic, organelles at electron microscopic, and tissue at light-microscopic resolution. A gap exists, however, when 3D tissue structure needs...

journal_title：PLoS biology

authors： Denk W,Horstmann H

更新日期：2004-11-01 00:00:00

abstract：:Better understanding of feeding behaviour will be vital in reducing obesity and metabolic syndrome, but we lack a standard model that captures the complexity of feeding behaviour. We construct an accurate stochastic model of rodent feeding at the bout level in order to perform quantitative behavioural analysis. Analys...

journal_title：PLoS biology

authors： McGrath TM,Spreckley E,Rodriguez AF,Viscomi C,Alamshah A,Akalestou E,Murphy KG,Jones NS

更新日期：2019-12-05 00:00:00

abstract：:A key component of working memory is the ability to remember multiple items simultaneously. To understand how the human brain maintains multiple items in memory, we examined direct brain recordings of neural oscillations from neurosurgical patients as they performed a working memory task. We analyzed the data to ident...

journal_title：PLoS biology

authors： Bahramisharif A,Jensen O,Jacobs J,Lisman J

更新日期：2018-08-15 00:00:00

abstract：:[This corrects the article DOI: 10.1371/journal.pbio.3000311.]. ...

journal_title：PLoS biology

authors： Simeon R,Jiang M,Chamoun-Emanuelli AM,Yu H,Zhang Y,Meng R,Peng Z,Jakana J,Zhang J,Feng H,Chen Z

更新日期：2019-10-04 00:00:00

abstract：:The genetic code provides the translation table necessary to transform the information contained in DNA into the language of proteins. In this table, a correspondence between each codon and each amino acid is established: tRNA is the main adaptor that links the two. Although the genetic code is nearly universal, sever...

journal_title：PLoS biology

authors： Abascal F,Posada D,Knight RD,Zardoya R

更新日期：2006-05-01 00:00:00

abstract：:How cell proliferation subsides as cells terminally differentiate remains largely enigmatic, although this phenomenon is central to the existence of multicellular organisms. Here, we show that GATA-1, the master transcription factor of erythropoiesis, forms a tricomplex with the retinoblastoma protein (pRb) and E2F-2....

journal_title：PLoS biology

authors： Kadri Z,Shimizu R,Ohneda O,Maouche-Chretien L,Gisselbrecht S,Yamamoto M,Romeo PH,Leboulch P,Chretien S

更新日期：2009-06-09 00:00:00