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The sodium-iodide symporter expression in placental tissue at different gestational age: an immunohistochemical study.

Abstract：

BACKGROUND:Iodide (I(-)) is crucial for foetal thyroid function. Foetal iodide results from maternal circulating iodide and from deiodination of iodothyronines within the placenta. The Na(+)/I(-) symporter (NIS) localized in placental cells appears to be involved in iodide exchange. Low NIS expression has been reported in trophoblast cells from the first trimester and pregnancy at term. AIMS:The aim of this study was to examine NIS expression by immunohistochemistry in the major components of human ovular tissue and placenta. MATERIALS AND METHODS:Formalin-fixed and paraffin-embedded specimens of placental tissue from the first trimester and at term were analysed. NIS expression was quantified as percentage of NIS-positive cells/total cells. NIS expression was also evaluated by real-time polymerase chain reaction (RT-PCR) in five first-trimester and five at-term placental specimens. RESULTS:In the first-trimester specimens heterogeneous NIS immunoreactivity was found in cyto-syncytiotrophoblast cells, with a range of NIS-positive cells from 5% to 80% (mean +/- SD 21.85 +/- 23.95), in mesenchymal and endothelial cells from 1% to 40% (14.5 +/- 11.16), in decidual cells from 5% to 40% (10.38 +/- 11.98) and in endometrial glands from 3% to 40% (21.86 +/- 13.93). In specimens from placenta at term, NIS-positive cyto-syncytiotrophoblast cells were between 5% and 40% (mean 17.85 +/- 18.15), mesenchymal and endothelial cells between 1% and 40% (13.67 +/- 12.16), decidual tissue between 5% and 30% (16.43 +/- 9.08), and endometrial glands between 3% and 40% (16.67 +/- 15.27). No significant differences in NIS expression were observed between the first trimester and placenta at term. A similar level of mRNA expression for the NIS gene was obtained by RT-PCR both in ovular material of the first trimester and in placenta at term. CONCLUSIONS:We found NIS to be expressed in various placental and ovular components and its expression to remain constant during pregnancy.

journal_name

Clin Endocrinol (Oxf)

journal_title

Clinical endocrinology

authors

Di Cosmo C,Fanelli G,Tonacchera M,Ferrarini E,Dimida A,Agretti P,De Marco G,Vitti P,Pinchera A,Bevilacqua G,Naccarato AG,Viacava P

doi

10.1111/j.1365-2265.2006.02577.x

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

544-8

issue

eissn

0300-0664

issn

1365-2265

pii

CEN2577

journal_volume

pub_type

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The sodium-iodide symporter expression in placental tissue at different gestational age: an immunohistochemical study.