Abstract:
BACKGROUND:Prostaglandin D(2) (PGD(2)), a major prostanoid produced by activated mast cells, has long been implicated in allergic diseases. PGD(2) demonstrates its effects through two G-protein-coupled receptors, DP and CRTH2. The PGD(2)/CRTH2 system mediates chemotaxis of eosinophils, basophils, and Th2 cells, which are involved in the induction of allergic inflammation. Although recent studies have shown that the specific receptors for PGD(2), DP, and CRTH2 are expressed in various human tissues, the role of PGD(2) is unknown in human bronchial epithelial cells. In this study, we investigated the expression and function of CRTH2/DP on NCI-H(292) and NHBE cells. METHOD:The CRTH2/DP expression was examined by RT-PCR and flow-cytometric analysis. NCI-H(292) and NHBE cells were cultured in the presence of various stimulants. The resulting supernatants were measured by ELISA. RESULTS:We demonstrated that PGD(2) induced production of IL-8 and GM-CSF in NCI-H(292) and NHBE cells. DK-PGD(2) (CRTH2 agonist) and latanoprost (FP, a prostaglandin F receptor, agonist) failed to augment the production of these cytokines. Pretreatment with ramatroban (CRTH2 antagonist) and AL8810 (FP antagonist) did not reduce the production of these cytokines. The PGD(2)-induced cytokine production was inhibited by pertussis toxin or specific inhibitors for MAP/ERK kinase (PD98059) and p38 MAP kinase (SB202190). CONCLUSION:These results suggest that PGD(2) is a potent inducer of IL-8 and GM-CSF production with MAP/ERK and p38 MAP kinase activation, but this is independent of CRTH2 activation.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Chiba T,Kanda A,Ueki S,Ito W,Kamada Y,Oyamada H,Saito N,Kayaba H,Chihara Jdoi
10.1159/000095436subject
Has Abstractpub_date
2006-01-01 00:00:00pages
300-7issue
3eissn
1018-2438issn
1423-0097pii
95436journal_volume
141pub_type
杂志文章abstract:BACKGROUND:Nitrosative and acid stress play an important role in the pathogenesis of asthma. The aim of this study was to evaluate whether, in asthmatics, a link exists between the concentrations of nitrite/nitrate, ammonia and pH values in exhaled breath condensate (EBC) and asthma severity, lung function, exhaled nit...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000335674
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:It is not known why some foods sensitizing via the gastrointestinal tract are prevalent allergenic foods and others are not. Eating habits, processing, and the food matrix have been suggested to influence the allergenicity of a given food. Factors related to protein structure, such as stability to digestion,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000321200
更新日期:2011-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the effectiveness of specific immunotherapy (SIT) management with allergoids in children with allergic asthma by monitoring changes in clinical parameters and inflammatory markers in exhaled breath. METHODS:The study population included 43 patients (24 males) of 6-14 ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000368832
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is increasing among the elderly. Staphylococcus aureus colonization is one of the most important aggravating factors in AD. OBJECTIVE:This study analyses the clinical features of AD in elderly patients and determines the pathogenic roles of staphylococcal superantigens in AD with low ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000329150
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:2-Arachidonoylglycerol (2-AG), an endogenous ligand for the cannabinoid receptors (CB1 and CB2), has been shown to exhibit a variety of cannabimimetic activities in vitro and in vivo. Recently, we found that human eosinophilic leukemia EoL-1 cells and human peripheral blood eosinophils express the CB2 recept...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000092704
更新日期:2006-01-01 00:00:00
abstract::We aimed to evaluate the therapeutic effect of nebulized beclomethasone dipropionate (nBDP) on both allergic asthma and rhinitis. In a randomized, double-blind, placebo-controlled study, 40 children (mean age 10.7 ± 2.1 years) with allergic asthma and rhinitis received either nBDP (daily dose of 800 µg, administered t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
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更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Allergic conjunctivitis is characterized by itchy, watery and swollen eyes which occur in response to exposure to seasonal or environmental allergens. The early phase reaction of allergic conjunctivitis is primarily mediated by mast cell degranulation while the late phase reaction is driven by Th2 cells and ...
journal_title:International archives of allergy and immunology
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doi:10.1159/000328769
更新日期:2012-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049498
更新日期:2001-09-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000265539
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abstract:INTRODUCTION:Cow's milk protein allergy (CMA) is the most common type of food allergy in childhood and exclusion diet is a challenge for patients. OBJECTIVE:The study aim was to investigate the frequency of tolerance to baked foods containing milk and evaluate immediate skin prick test (SPT) and specific IgEs for diff...
journal_title:International archives of allergy and immunology
pub_type: 新闻
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journal_title:International archives of allergy and immunology
pub_type: 新闻
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000090385
更新日期:2006-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237621
更新日期:1997-08-01 00:00:00
abstract::Peritoneal mast cells of Syrian hamsters release histamine to the action of concanavalin A (Con A) in dose-dependent fashion. The rate of release was very rapid in the first seconds of cell activation and completed in 60 s after the challenge. Morphological changes concomitant to the lectin treatment, followed by elec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236113
更新日期:1992-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000488329
更新日期:2018-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000085427
更新日期:2005-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237099
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Loss-of-function mutations of the filaggrin (FLG) gene cause an impaired skin barrier and increase the risk of atopic dermatitis. Interestingly, FLG mutations have also been found to be associated with a high risk of peanut allergy. OBJECTIVE:We investigated the association of FLG mutations with self-report...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000345949
更新日期:2013-01-01 00:00:00
abstract::Oral immunotherapy (OIT) is a significant focus of treatment of food allergy. OIT appears to be effective in inducing desensitization, however, patients receiving OIT frequently developmild/moderate symptoms during the therapy. It has not been clearly established whether the clinical tolerance induced by OIT resembles...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The digestive system of Dermatophagoides farinae remains poorly documented. METHODS:In this study, a three-dimensional (3-D) model of the alimentary canal of D. farinae was built for the first time based on hematoxylin-eosin staining, 76 cross-sections of the adult mite and 3-D reconstruction technology. R...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000115890
更新日期:2008-01-01 00:00:00
abstract::Peritoneal eosinophilia was induced in rats using Ascaris suum extract as an antigen, and characteristics of granule proteins of eosinophils collected from the peritoneal cavity were investigated. Peritoneal eosinophilia was induced by injection of the antigen solution into the peritoneal cavity of the immunized rats ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237111
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Mast cell-released chemical mediators such as histamine, leukotriene (LT) C(4) and prostaglandin (PG) D(2) lead to the onset of allergic disorders. ATP provided from glycolysis is essential for histamine release and LTC(4) secretion from mast cells upon Fc epsilon RI cross-linking, indicating that glucose is...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000312122
更新日期:2010-01-01 00:00:00
abstract::Ever since the realization that T lymphocytes are key players in the defense against pathogens and tumors, a major aim of immunologists has been to understand the relationship between the functional and migratory properties of antigen-specific T cells. The current paradigm proposes that T cells follow organ-specific t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000074896
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:The classical clinical manifestation of untreated immunoglobulin deficiency comprises predominantly recurrent and complicated respiratory tract infections. Before the 1980s, little was known about the clinical manifestation of immunodeficiency in the general medical population, and also the availability of s...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000301584
更新日期:2010-01-01 00:00:00
abstract::Delayed drug allergy reactions (DDAR) are potentially fatal. Certain human leukocyte antigen (HLA) alleles have been associated with delayed allergy reactions following the administration of particular drugs. Examples are HLA-B*57:01 (abacavir), HLA-B*15:02/HLA-A*31:01 (carbamazepine), and HLA-B*58:01 (allopurinol). B...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000448217
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Several studies have examined the relationship of asthma with serum dyslipidemia and reported positive, negative or no association. Most studies were limited by their cross-sectional design and the wide age range of the participants. In a cohort of children in Cyprus, we explored the association of asthma wi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000368405
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Natural rubber latex (NRL) products are complex mixtures consisting of different allergenic components. Among them, Hev b 1 belongs to the important and well-characterized ones. To quantify the relevant allergen Hev b 1 in NRL products, a two-site monoclonal antibody (mAb)-based assay was developed. METHODS...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024449
更新日期:2000-11-01 00:00:00