当前位置： SCI文献检索 > EUROPEAN JOURNAL OF PHARMACOLOGY期刊下所有文献 > Neuroprotective effects of thymoquinone against transient forebrain ischemia in the rat hippocampus.

Neuroprotective effects of thymoquinone against transient forebrain ischemia in the rat hippocampus.

Abstract：

:Increasing evidence demonstrates that oxidative stress plays an important role in brain injury in experimental models of brain ischemia. Thymoquinone, the main constituents of the volatile oil from Negella sativa seeds, is reported to possess strong antioxidant properties. Hence, the present study was undertaken to evaluate the neuroprotective effect of thymoquinone against transient forebrain ischemia-induced neuronal damage in the rat hippocampus. Rats were divided randomly into five groups: control, sham, ischemia, thymoquinone and ischemia+thymoquinone. Transient forebrain ischemia was induced with bilateral occlusion of both common carotid arteries for 10 min followed by 7 days of reperfusion. Thymoquinone was administered (5 mg/kg/day p.o.) 5 days before ischemia and continued during the reperfusion time. Animals were sacrificed, and brain tissues were isolated for histopathological examination. Hippocampal tissues were also used for determination of malondialdehyde levels, an end product of lipid peroxidation; glutathione (GSH) levels, a key antioxidant and the activities of the antioxidant enzymes catalase and superoxide dismutase (SOD). Thymoquinone and its metabolite thymohydroquinone were tested as inhibitors of the in vitro non-enzymatic lipid peroxidation induced by iron-ascorbate in the hippocampal homogenate. Forebrain ischemia-reperfusion neural injury in rats was demonstrated by histopathological observation, which revealed significant neural cell death in the hippocampus CA1 area 7 days post-ischemia (77% cell loss). Additionally, forebrain ischemia-reperfusion oxidative injury in rats was demonstrated by a significant increase in malondialdehyde and a significant decrease in GSH contents, catalase and SOD activities in the hippocampal tissue compared to the control or sham-operated groups. Pretreatment of thymoquinone attenuated forebrain ischemia-induced neuronal damage manifested by significantly decreasing the number of dead hippocampal neuronal cells (24% in thymoquinone-treated versus 77% for ischemia, P<0.001), which confirm the protective role of thymoquinone in ischemia-reperfusion injury. Also, pretreatment of ischemic rats with thymoquinone decreased the elevated levels of malondialdehyde and increased GSH contents, catalase and SOD activities to normal levels. Thymoquinone and thymohydroquinone inhibited the in vitro non-enzymatic lipid peroxidation in hippocampal homogenate induced by iron-ascorbate. The IC50 for thymoquinone and thymohydroquinone were found to be 12 and 3 microM respectively. This suggests that the protection of thymoquinone and its metabolite involve increased resistance to oxidative stress. In conclusion, thymoquinone is effective in protecting rats against transient forebrain ischemia-induced damage in the rat hippocampus. This spectacular protection makes thymoquinone a promising agent in pathologies implicating neurodegenaration such as cerebral ischemia.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Al-Majed AA,Al-Omar FA,Nagi MN

doi

10.1016/j.ejphar.2006.05.046

subject

Has Abstract

pub_date

2006-08-14 00:00:00

pages

40-7

issue

1-3

eissn

0014-2999

issn

1879-0712

pii

S0014-2999(06)00573-5

journal_volume

543

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF PHARMACOLOGY文献大全
  • PM101: a cyclodextrin-based intravenous formulation of amiodarone devoid of adverse hemodynamic effects.

    abstract：:Intravenous amiodarone (Amiodarone i.v.) is widely used to treat cardiac arrhythmias. The most frequent clinical adverse event associated with Amiodarone i.v. administration is systemic hypotension which has been attributed to the cosolvents used in the formulation, polysorbate 80 and benzyl alcohol. To minimize hypot...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2009.02.009

    authors： Cushing DJ,Kowey PR,Cooper WD,Massey BW,Gralinski MR,Lipicky RJ

    更新日期：2009-04-01 00:00:00

  • Oral administration of quercetin inhibits bone loss in rat model of diabetic osteopenia.

    abstract：:Diabetic osteopenia can result in an increased incidence of bone fracture and a delay in fracture healing. Quercetin, one of the most widely distributed flavonoids in plants, possesses antioxidant property and beneficial effect on osteoporosis in ovariectomized mice. All these properties make quercetin a potential can...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2011.08.014

    authors： Liang W,Luo Z,Ge S,Li M,Du J,Yang M,Yan M,Ye Z,Luo Z

    更新日期：2011-11-16 00:00:00

  • Unraveling the identity of benzodiazepine binding sites in rat hipppocampus and olfactory bulb.

    abstract：:The goals of the work reported here were (i) to identify distinct GABA(A)/benzodiazepine receptors in the rat hippocampus and olfactory bulb using receptor binding assays, and (ii) to determine the affinities and selectivities of benzodiazepine receptor ligands from structurally diverse chemical families at each site ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(00)00398-8

    authors： Lameh J,Wang P,Elgart D,Meredith D,Shafer SL,Loew GH

    更新日期：2000-07-21 00:00:00

  • Inhibitors of 1,2-diacylglycerol kinase potentiate the TRH-induced stimulation of Ca2+-activated K+ current.

    abstract：:Transient activation of the outward K+ current caused by a rise in the cytosolic free Ca2+ concentration, [Ca2+]i was the predominant change in plasma membrane ion flux during the first phase of thyrotropin-releasing hormone (TRH) action on pituitary cells. Following the intracellular application of inhibitors of 1,2-...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0922-4106(89)90046-1

    authors： Mollard P,Vacher P,Dufy B,Winiger BP,Schlegel W

    更新日期：1989-03-07 00:00:00

  • Dopamine agonistic potency of two novel prolactin release-inhibiting ergolines.

    abstract：:Two novel 8 alpha-amino ergolines (CH 29-717 and CU 32-085) have been shown to inhibit secretion of prolactin in rats in vivo. However, when tested for dopaminomimetic potency on pituitary cell culture preparations in vitro, CH 29-717 inhibited prolactin release with an IC50 = 4 X 10(-9) M. In the same model the 1-met...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(84)90167-5

    authors： Markó M

    更新日期：1984-06-01 00:00:00

  • Effect of 3-isobutyl-1-methylxanthine and zaprinast on non-adrenergic non-cholinergic relaxation in the rat gastric fundus.

    abstract：:Vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) have been proposed as inhibitory non-adrenergic non-cholinergic (NANC) neurotransmitters in the rat gastric fundus. The smooth muscle relaxant actions of VIP and NO are medaited by cAMP and cGMP, respectively; therefore the effect of inhibitors of phosphodi...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(92)90421-y

    authors： Barbier AJ,Lefebvre RA

    更新日期：1992-01-21 00:00:00

  • Vascular effects of capsaicin in isolated perfused rat mesenteric bed.

    abstract：:The effects of intra- and extraluminal capsaicin administration were evaluated in isolated perfused rat mesenteric bed. Capsaicin (10 nM-1 microM) produced a potent concentration-dependent relaxation of the tonic vasoconstriction induced by norepinephrine (1 microM) but not by high-K+ (60 mM). The capsaicin-induced re...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(88)90559-6

    authors： Manzini S,Perretti F

    更新日期：1988-03-29 00:00:00

  • Evidence for a role of nitric oxide in the corticotropin-releasing factor release induced by interleukin-1 beta.

    abstract：:Interleukin-1 beta stimulates corticotropin-releasing factor (CRF) secretion from the hypothalamus involving the activation of prostaglandins. This study investigated the possibility that nitric oxide (NO) acts as a mediator of interleukin-1-induced CRF release. An in vitro rat hypothalami continuous perifusion system...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)00700-h

    authors： Sandi C,Guaza C

    更新日期：1995-02-14 00:00:00

  • Halothane counteracts acetylcholine-induced increase in Ca2+ sensitivity of the contractile apparatus in airway smooth muscle.

    abstract：:The direct relaxing effect of halothane on airway smooth muscle has been reported to involve the reduction of the cytosolic Ca2+ concentration ([Ca2+]i) and the [Ca2+]i-independent inhibitory mechanism. To clarify the extent of the contribution of these mechanisms, the effect of halothane on the [Ca2+]i-tension relati...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(96)00617-6

    authors： Kai T,Takahashi S,Kanaide H

    更新日期：1996-11-21 00:00:00

  • Emodin augments calcium activated chloride channel in colonic smooth muscle cells by Gi/Go protein.

    abstract：:Emodin is a natural anthraquinone in rhubarb. It has been identified as a prokinetic drug for gastrointestinal motility in Chinese traditional medicine. Emodin contracts smooth muscle by increasing the concentration of intracellular Ca(2+). In many smooth muscles, increasing intracellular Ca(2+) activates Ca(2+)-activ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2009.04.045

    authors： Xu L,Ting-Lou,Lv N,Zhu X,Chen Y,Yang J

    更新日期：2009-08-01 00:00:00

  • Comparison between dopamine transporter affinity and self-administration potency of local anesthetics in rhesus monkeys.

    abstract：:Local anesthetics bind to dopamine transporters and inhibit dopamine uptake in rodent brain. Additionally, local anesthetics are self-administered in rhesus monkeys. The present study determined binding affinities of cocaine and five local anesthetics at dopamine transporters in rhesus monkey brain, and compared bindi...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00967-4

    authors： Wilcox KM,Paul IA,Woolverton WL

    更新日期：1999-02-19 00:00:00

  • Genital grooming and emesis induced by vanilloids in Suncus murinus, the house musk shrew.

    abstract：:The potential of resiniferatoxin and capsaicin to modulate emesis and genital grooming was investigated in Suncus murinus. Resinifertoxin (3-30 nmol, i.c.v.), E-capsaicin (10-100 nmol, i.c.v.) and Z-capsaicin (100 nmol, i.c.v.) induced emesis (P<0.05) and subsequently antagonised the emetic response induced by intraga...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(01)01041-x

    authors： Rudd JA,Wai MK

    更新日期：2001-06-22 00:00:00

  • Enalapril, a new nonsulfhydryl angiotensin converting enzyme inhibitor, does not potentiate morphine analgesia.

    abstract：:Sprague-Dawley rats received oral doses of enalapril maleate (5 mg/kg), a potent, nonsulfhydryl, angiotensin converting enzyme inhibitor, or saline. Sixty min later, morphine sulfate, 5 mg/kg, or saline was injected subcutaneously. Response to a thermal stimulus was monitored before and up to 5 h ter morphine injectio...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(84)90607-1

    authors： Mojaverian P,Swanson BN,Ferguson RK

    更新日期：1984-02-17 00:00:00

  • Effect of olanzapine on scopolamine induced deficits in differential reinforcement of low rate 72s (DRL-72s) schedule in rats: involvement of the serotonergic receptors in restoring the deficits.

    abstract：:Scopolamine, a non-selective muscarinic receptor antagonist has widespread central nervous system effects. Muscarinic receptors located in the central nervous system play a vital role in the modulation of impulsivity. The objective of the current study was to evaluate the effect of scopolamine on impulsivity using dif...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.10.005

    authors： Jayarajan P,Nirogi R,Shinde A

    更新日期：2013-11-15 00:00:00

  • Neurokinins produce selective venoconstriction via NK-3 receptors in the rat mesenteric vascular bed.

    abstract：:The vasoactive properties of the neurokinins (substance P (SP), neurokinin A (NKA), neurokinin B (NKB)) and some selective analogues were assessed in the arterial and venous mesenteric beds of the rat. Although both sides of the mesenteric vasculature displayed endothelium-dependent relaxation in response to acetylcho...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(91)90860-s

    authors： D'Orléans-Juste P,Claing A,Télémaque S,Warner TD,Regoli D

    更新日期：1991-11-12 00:00:00

  • Tracheal epithelium releases a vascular smooth muscle relaxant factor: demonstration by bioassay.

    abstract：:Acetylcholine caused no relaxation of a rubbed strip from rabbit aorta (RASR) which was precontracted with phenylephrine. When the same strip was taken into epithelium-intact guinea-pig trachea, acetylcholine produced a slow-developing relaxation which was antagonized by atropine but not by propranolol, indomethacin, ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(86)90586-8

    authors： Ilhan M,Sahin I

    更新日期：1986-11-19 00:00:00

  • Felbamate reduces hypoxic-ischemic brain damage in vivo.

    abstract：:The neuroprotective effects of felbamate were tested in a model of incomplete cerebral ischemia and hypoxia in 7-day-old rat pups. Felbamate pretreatment (300 mg/kg) reduced the surface of infarcted cortex following bilateral carotid ligation, by 42-49% compared to saline and dimethylsulfoxide (DMSO) controls, respect...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(92)90343-3

    authors： Wasterlain CG,Adams LM,Hattori H,Schwartz PH

    更新日期：1992-03-03 00:00:00

  • Modulation of endothelial prostaglandin synthesis by corticotropin releasing factor and antagonists.

    abstract：:Corticotropin releasing factor (CRF) is a hypothalamic hormone that also displays autocrine/paracrine roles at peripheral sites. High concentrations of CRF have been identified in endothelial cells and other inflammatory tissues. We investigated the effects of CRF and antagonists in the regulation of prostaglandin syn...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00416-6

    authors： Fleisher-Berkovich S,Rimon G,Danon A

    更新日期：1998-07-24 00:00:00

  • Selective inhibition of the inducible nitric oxide synthase by aminoguanidine.

    abstract：:Overproduction of the free radical nitric oxide (NO) has been implicated in the pathogenesis of a variety of inflammatory and immunologically mediated diseases as well as complications of diabetes. In the present study we have demonstrated that aminoguanidine selectively inhibits the cytokine-inducible isoform of NO s...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(93)90357-n

    authors： Misko TP,Moore WM,Kasten TP,Nickols GA,Corbett JA,Tilton RG,McDaniel ML,Williamson JR,Currie MG

    更新日期：1993-03-16 00:00:00

  • Adenosine inhibits efferent function of extrinsic capsaicin-sensitive sensory nerves in the enteric nervous system.

    abstract：:Capsaicin (1-3 microM) and electrical stimulation of mesenteric nerves in the presence of hexamethonium and guanethidine antidromically stimulate extrinsic sensory nerve fibers to produce a specific slow depolarizing response of myenteric neurons and a contractile response of muscles in the isolated guinea-pig ileum, ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(93)90092-v

    authors： Takaki M,Namba T,Fujii W,Suga H

    更新日期：1993-06-11 00:00:00

  • Evaluation of delta receptor mediation of supraspinal opioid analgesia by in vivo protection against the beta-funaltrexamine antagonist effect.

    abstract：:The involvement of delta opioid receptors in supraspinal analgesia was investigated. With this aim, opioids that produced analgesia in the tail immersion test were administered i.c.v. to mice a few minutes before the irreversible antagonist, beta-funaltrexamine (beta-FNA). Protection of the respective analgesic effect...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90038-1

    authors： Sánchez-Blázquez P,Garzón J

    更新日期：1989-01-02 00:00:00

  • Impairment of adenylyl cyclase signal transduction in mecobalamin-deficient rats.

    abstract：:This study examined alterations in the beta-adrenoceptor-G5-adenylyl cyclase system in cerebral cortex membranes from vitamin B12-deficient rats fed a diet lacking vitamin B12 (mecobalamin) for 15 weeks. Basal, 5(7)-guanylylimidodiphosphate (GppNHp)-, isoproterenol-, and forskolin-stimulated adenylyl cyclase activitie...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0922-4106(95)90076-4

    authors： Hatta S,Watanabe M,Ikeda H,Kamada H,Saito T,Ohshika H

    更新日期：1995-11-30 00:00:00

  • Effect of two dopamine agonists, TL-99 and 3-PPP, on prolactin secretion in the rat.

    abstract：:TL-99 and 3-PPP, two putative dopamine autoreceptor agonists, effectively suppressed serum prolactin (PRL) concentrations in rats treated with alpha-methyltyrosine. In addition, the secretion of PRL from anterior pituitary tissue under in vitro conditions was significantly inhibited by TL-99 and 3-PPP. These findings ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(83)90565-4

    authors： Gudelsky GA,Passaro E,Meltzer HY

    更新日期：1983-06-17 00:00:00

  • Short chain bombesin pseudopeptides with potent bombesin receptor antagonist activity in rat and guinea pig pancreatic acinar cells.

    abstract：:A series of potent bombesin antagonists based on the reduced C-terminal peptide bond modification which in the past resulted in the first really potent antagonists are compared for effects on bombesin-stimulated amylase release from and binding to rat and guinea pig pancreatic acini. It was found that the original mem...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(90)94109-b

    authors： Coy D,Wang LH,Jiang NY,Jensen R

    更新日期：1990-11-06 00:00:00

  • Antagonism of the anxiolytic action of diazepam and chlordiazepoxide by the novel imidazopyridines, EMD 39593 and EMD 41717.

    abstract：:The imidazopyridines EMD 35993 and EMD 41717 antagonized the anticonflict actions of diazepam and chlordiazepoxide in rodent models which are predictive for anxiolytic action in man. In contrast to other described benzodiazepine antagonists, these compounds did not antagonize either the anticonvulsant or muscle relaxa...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(83)90582-4

    authors： Skolnick P,Paul S,Crawley J,Lewin E,Lippa A,Clody D,Irmscher K,Saiko O,Minck KO

    更新日期：1983-04-08 00:00:00

  • Mechanisms of simvastatin myotoxicity: The role of autophagy flux inhibition.

    abstract：:Statins are some of the most widely used drugs worldwide, but one of their major side effects is myotoxicity. Using mouse myoblast (C2C12) and human alveolar rhabdomyosarcoma cell lines (RH30) in both 2-dimensional (2D) and 3-dimensional (3D) cell culture, we investigated the mechanisms of simvastatin's myotoxicity. W...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2019.172616

    authors： Emami A,Shojaei S,da Silva Rosa SC,Aghaei M,Samiei E,Vosoughi AR,Kalantari F,Kawalec P,Thliveris J,Sharma P,Zeki AA,Akbari M,Gordon JW,Ghavami S

    更新日期：2019-11-05 00:00:00

  • Ramatroban, a TP receptor antagonist, improves vascular responses to acetylcholine in hypercholesterolemic rabbits in vivo.

    abstract：:Recent studies show that 8-iso-prostaglandin F(2alpha), a member of F(2)-isoprostane family, acts as a vasoconstrictor via TP receptor activation; and its local release may contribute to an abnormal vasomotor tone associated with hypercholesterolemia. The purpose of this study was to examine whether ramatroban, a TP r...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(03)01626-1

    authors： Ishizuka T,Matsui T,Kurita A

    更新日期：2003-05-02 00:00:00

  • Osthole suppresses seizures in the mouse maximal electroshock seizure model.

    abstract：:The aim of this study was to determine the anticonvulsant effects of osthole {[7-methoxy-8-(3-methyl-2-butenyl)-2H-1-benzopyran-2-one]--a natural coumarin derivative} in the mouse maximal electroshock-induced seizure model. The antiseizure effects of osthole were determined at 15, 30, 60, and 120 min after its systemi...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2009.02.022

    authors： Luszczki JJ,Andres-Mach M,Cisowski W,Mazol I,Glowniak K,Czuczwar SJ

    更新日期：2009-04-01 00:00:00

  • Drug-eluting stents: sirolimus and paclitaxel differentially affect cultured cells and injured arteries.

    abstract：:Sirolimus and paclitaxel eluted from stents inhibit cell proliferation and other cellular processes by dramatically different mechanisms. In this study, the effects of sirolimus and paclitaxel on cultured human coronary artery smooth muscle and endothelial cell function or cell cycle changes in balloon-injured arterie...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2005.09.042

    authors： Parry TJ,Brosius R,Thyagarajan R,Carter D,Argentieri D,Falotico R,Siekierka J

    更新日期：2005-11-07 00:00:00

  • MDL 74,180 reduces cerebral infarction and free radical concentrations in rats subjected to ischaemia and reperfusion.

    abstract：:The protective effect of MDL 74,180 (2,3-dihydro-2,2,4,6, 7-pentamethyl-3-(4-methylpiperazino)-methyl-1-benzofuran-5-ol dihydrochloride) and alpha-tocopherol analogue free radical scavenger, against cerebral ischaemia and reperfusion in conscious rats has been demonstrated. Tissue damage following middle cerebral arte...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00807-1

    authors： Cowley DJ,Lukovic L,Petty MA

    更新日期：1996-03-18 00:00:00