Database resources of the National Center for Biotechnology Information.

abstract：:The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginy...

journal_title：Nucleic acids research

authors： Laigle A,Chinsky L,Turpin PY

更新日期：1982-03-11 00:00:00

abstract：:The POU domain transcription factor, Oct-2, is essential for the B cell-specific expression of CD36 in mouse B cells. In order to determine how Oct-2 mediates expression of CD36 in B cells, we cloned and analysed the mouse CD36 promoter. In contrast to the human CD36 promoter, the mouse promoter contains a consensus o...

journal_title：Nucleic acids research

authors： Shore P,Dietrich W,Corcoran LM

更新日期：2002-04-15 00:00:00

abstract：:Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional charact...

journal_title：Nucleic acids research

authors： Benders GA,Noskov VN,Denisova EA,Lartigue C,Gibson DG,Assad-Garcia N,Chuang RY,Carrera W,Moodie M,Algire MA,Phan Q,Alperovich N,Vashee S,Merryman C,Venter JC,Smith HO,Glass JI,Hutchison CA 3rd

更新日期：2010-05-01 00:00:00

abstract：:We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...

journal_title：Nucleic acids research

authors： Yu BJ,Kang KH,Lee JH,Sung BH,Kim MS,Kim SC

更新日期：2008-08-01 00:00:00

abstract：:We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist t...

journal_title：Nucleic acids research

authors： Mytelka DS,Chamberlin MJ

更新日期：1996-07-15 00:00:00

abstract：:Lens epithelium-derived growth factor/p75 (LEDGF/p75) is a transcriptional coactivator involved in stress response, autoimmune disease, cancer and HIV replication. A fusion between the nuclear pore protein NUP98 and LEDGF/p75 has been found in human acute and chronic myeloid leukemia and association of LEDGF/p75 with ...

journal_title：Nucleic acids research

authors： De Rijck J,Bartholomeeusen K,Ceulemans H,Debyser Z,Gijsbers R

更新日期：2010-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the stability of highly repetitive rDNA array is maintained through transcriptional silencing. Recently, a β-1,3-glucanosyltransferase Gas1 has been shown to play a significant role in the regulation of transcriptional silencing in S. cerevisiae. Here, we show that the gas1Δ mutation incre...

journal_title：Nucleic acids research

authors： Ha CW,Kim K,Chang YJ,Kim B,Huh WK

更新日期：2014-07-01 00:00:00

abstract：:Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific nucleases such as Transcription Activator-Like Effectors Nucleases (TALENs) and Cas9-gRNA allows...

journal_title：Nucleic acids research

authors： Yang L,Guell M,Byrne S,Yang JL,De Los Angeles A,Mali P,Aach J,Kim-Kiselak C,Briggs AW,Rios X,Huang PY,Daley G,Church G

更新日期：2013-10-01 00:00:00

abstract：:A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction...

journal_title：Nucleic acids research

authors： Watkins PC,Tanzi RE,Gibbons KT,Tricoli JV,Landes G,Eddy R,Shows TB,Gusella JF

更新日期：1985-09-11 00:00:00

abstract：:The last 82 nucleotides of the 6.3 kb genomic RNA of plant turnip yellow mosaic virus (TYMV), the so-called 'tRNA-like' domain, presents functional, structural and primary sequence homologies with canonical tRNAs. In particular, one of the stem-loops resembles the TPsi(pseudouridine)-branch of tRNA, except for the pre...

journal_title：Nucleic acids research

authors： Becker HF,Motorin Y,Florentz C,Giegé R,Grosjean H

更新日期：1998-09-01 00:00:00

abstract：:The tumor suppressor gene, p53, is rarely mutated in neuroblastomas (NB) at the time of diagnosis, but its dysfunction could result from a nonfunctional conformation or cytoplasmic sequestration of the wild-type p53 protein. However, p53 mutation, when it occurs, is found in NB tumors with drug resistance acquired ove...

journal_title：Nucleic acids research

authors： Goldschneider D,Horvilleur E,Plassa LF,Guillaud-Bataille M,Million K,Wittmer-Dupret E,Danglot G,de Thé H,Bénard J,May E,Douc-Rasy S

更新日期：2006-01-01 00:00:00

abstract：:Three databases that provide data on transcriptional regulation are described. TRANSFAC is a database on transcription factors and their DNA binding sites. TRRD (Transcription Regulatory Region Database) collects information about complete regulatory regions, their regulation properties and architecture. COMPEL compri...

journal_title：Nucleic acids research

authors： Wingender E,Kel AE,Kel OV,Karas H,Heinemeyer T,Dietze P,Knüppel R,Romaschenko AG,Kolchanov NA

更新日期：1997-01-01 00:00:00

abstract：:We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidi...

journal_title：Nucleic acids research

authors： Nilsson AN,Emilsson G,Nyberg LK,Noble C,Stadler LS,Fritzsche J,Moore ER,Tegenfeldt JO,Ambjörnsson T,Westerlund F

更新日期：2014-09-01 00:00:00

abstract：:Upstream open reading frames (uORFs) latent in mRNA transcripts are thought to modify translation of coding sequences by altering ribosome activity. Not all uORFs are thought to be active in such a process. To estimate the impact of uORFs on the regulation of translation in humans, we first circumscribed the universe ...

journal_title：Nucleic acids research

authors： McGillivray P,Ault R,Pawashe M,Kitchen R,Balasubramanian S,Gerstein M

更新日期：2018-04-20 00:00:00

abstract：:We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...

journal_title：Nucleic acids research

authors： Asai K,Kiryu H,Hamada M,Tabei Y,Sato K,Matsui H,Sakakibara Y,Terai G,Mituyama T

更新日期：2008-07-01 00:00:00

abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is Europe's primary nucleotide-sequence repository. The ENA consists of three main databases: the Sequence Read Archive (SRA), the Trace Archive and EMBL-Bank. The objective of ENA is to support and promote the use of nucleotide sequencing as an experimen...

journal_title：Nucleic acids research

authors： Leinonen R,Akhtar R,Birney E,Bower L,Cerdeno-Tárraga A,Cheng Y,Cleland I,Faruque N,Goodgame N,Gibson R,Hoad G,Jang M,Pakseresht N,Plaister S,Radhakrishnan R,Reddy K,Sobhany S,Ten Hoopen P,Vaughan R,Zalunin V,Cochr

更新日期：2011-01-01 00:00:00

abstract：:Vascular endothelial growth factor A (VEGF-A) is a master regulator of angiogenesis, vascular development and function. In this study we investigated the transcriptional regulation of VEGF-A-responsive genes in primary human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using gen...

journal_title：Nucleic acids research

authors： Kaikkonen MU,Niskanen H,Romanoski CE,Kansanen E,Kivelä AM,Laitalainen J,Heinz S,Benner C,Glass CK,Ylä-Herttuala S

更新日期：2014-11-10 00:00:00

abstract：:We used 2D protein gel electrophoresis and DNA microarray technologies to systematically analyze genes under glucose repression in B:acillus subtilis. In particular, we focused on genes expressed after the shift from glycolytic to gluconeogenic at the middle logarithmic phase of growth in a nutrient sporulation medium...

journal_title：Nucleic acids research

authors： Yoshida K,Kobayashi K,Miwa Y,Kang CM,Matsunaga M,Yamaguchi H,Tojo S,Yamamoto M,Nishi R,Ogasawara N,Nakayama T,Fujita Y

更新日期：2001-02-01 00:00:00

abstract：:Active DNA demethylation is crucial for epigenetic control, but the underlying enzymatic mechanisms are incompletely understood. REPRESSOR OF SILENCING 1 (ROS1) is a 5-methylcytosine (5-meC) DNA glycosylase/lyase that initiates DNA demethylation in plants through a base excision repair process. The enzyme binds DNA no...

journal_title：Nucleic acids research

authors： Parrilla-Doblas JT,Ponferrada-Marín MI,Roldán-Arjona T,Ariza RR

更新日期：2013-10-01 00:00:00

abstract：:We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by compa...

journal_title：Nucleic acids research

authors： Meyerhof W,Klinger-Mitropoulos S,Stalder J,Weber R,Knöchel W

更新日期：1984-10-25 00:00:00

abstract：:Human Hepatitis B Virus (HBV) RNAs contain a cis -acting sequence, the post-transcriptional regulatory element (HPRE), which facilitates the cytoplasmic localization of intronless transcripts. Our previous studies have shown that the HPRE is composed of at least two independent sub-elements, HPREalpha and HPREbeta, wh...

journal_title：Nucleic acids research

authors： Smith GJ 3rd,Donello JE,Lück R,Steger G,Hope TJ

更新日期：1998-11-01 00:00:00

abstract：:Upon endocytosis, most oligodeoxynucleotides (ODNs) accumulate in vesicular compartments; a tiny number of them cross the vesicle membrane, reach the cytosol and by passive diffusion enter the nucleus where they are entrapped. So far, the compartment in which an antisense ODN interacts with its mRNA target has not bee...

journal_title：Nucleic acids research

authors： Meunier L,Mayer R,Monsigny M,Roche AC

更新日期：1999-07-01 00:00:00

abstract：:Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo cell-free protein synthesis system shows a heterogeneous but equal distribution of these abundant translatable mRNAs in...

journal_title：Nucleic acids research

authors： Saunders CA,Bostian KA,Halvorson HO

更新日期：1980-09-11 00:00:00

abstract：:Two similar, but not identical, models have been proposed for the amino acid-base pair contacts in the CAP-DNA complex ('Model I,' Weber, I. and Steitz, T., Proc. Natl. Acad. Sci. USA, 81, 3973-3977, 1984; 'Model II,' Ebright, et al., Proc. Natl. Acad. Sci. USA, 81, 7274-7278, 1984). One difference between the two mod...

journal_title：Nucleic acids research

authors： Gunasekera A,Ebright YW,Ebright RH

更新日期：1990-12-11 00:00:00

abstract：:The initial genomic response to serum growth factors is the transcriptional activation of a set of immediate-early genes. Serum-induced transcriptional activation of several of these genes involves the formation of a ternary complex that includes the serum response factor (SRF), a 62 kDa ternary complex factor (TCF) a...

journal_title：Nucleic acids research

authors： Latinkić BV,Zeremski M,Lau LF

更新日期：1996-04-01 00:00:00

abstract：:Homologous recombination (HR) is a highly accurate mechanism of DNA repair that can be exploited for homology-directed gene targeting. Since in most cell types HR occurs very infrequently (approximately 10(-6) to 10(-8)), its practical application has been largely restricted to specific experimental systems that allow...

journal_title：Nucleic acids research

authors： van Nierop GP,de Vries AA,Holkers M,Vrijsen KR,Gonçalves MA

更新日期：2009-09-01 00:00:00

abstract：:A limited number of Methicillin-resistant Staphylococcus aureus (MRSA) clones are responsible for MRSA infections worldwide, and those of different lineages carry unique Type I restriction-modification (RM) variants. We have identified the specific DNA sequence targets for the dominant MRSA lineages CC1, CC5, CC8 and ...

journal_title：Nucleic acids research

authors： Roberts GA,Houston PJ,White JH,Chen K,Stephanou AS,Cooper LP,Dryden DT,Lindsay JA

更新日期：2013-08-01 00:00:00

abstract：:Mutations in immunoglobulin µ-binding protein 2 (Ighmbp2) cause distal spinal muscular atrophy type 1 (DSMA1), an autosomal recessive disease that is clinically characterized by distal limb weakness and respiratory distress. However, despite extensive studies, the mechanism of disease-causing mutations remains elusive...

journal_title：Nucleic acids research

authors： Lim SC,Bowler MW,Lai TF,Song H

更新日期：2012-11-01 00:00:00

abstract：:Oligodeoxyribonucleotides containing N4-methoxycytosine (mo4C), N4-methoxy-5-methylcytosine (mo4m5C) and other base-analogues were synthesised and used to compare the stabilities of duplexes containing mo4C.A and mo4C.G base pairs with those containing normal and mismatch pairs. The Tm values and other thermodynamic p...

journal_title：Nucleic acids research

authors： Anand NN,Brown DM,Salisbury SA

更新日期：1987-10-26 00:00:00

abstract：:Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The ...

journal_title：Nucleic acids research

authors： Yang D,Tan Z,Mi Y,Wei B

更新日期：2017-04-07 00:00:00