Abstract：
:Pituicytes, the astrocytic glial cells of the neural lobe, are known to secrete interleukin-6 and nitric oxide upon stimulation with various inflammatory mediators, i.e. interleukin-1beta. Nitric oxide is described to modulate the secretion of interleukin-6 in various cell types. The aim of the present study was to investigate the effect of nitric oxide on interleukin-1beta induced interleukin-6 secretion. Furthermore the effect of interferon-gamma on interleukin-6 and nitric oxide release was investigated. Cultures of pituicytes were prepared of neural lobes from male mice. The effect of interleukin-1beta and interferon-gamma on interleukin-6 and nitric oxide secretion was investigated in pituicytes cultured for 14 days. The secretion of interleukin-6 and nitric oxide was determined after 24 h of stimulation. Pituicytes secrete interleukin-6 upon stimulation with interleukin-1beta dose dependently but did not induce any detectable nitric oxide release. Co-stimulation with interferon-gamma and interleukin-1beta induced a significant nitric oxide release. In addition interferon-gamma inhibits interleukin-1beta induced interleukin-6 secretion dose dependently. The observed effect of interferon-gamma on interleukin-6 secretion was not affected by the specific inducible nitric oxide synthase inhibitor 1400W (N-(3-[aminomethyl]benzyl)acetamidine). Furthermore interferon-gamma dose dependently inhibits unstimulated interleukin-6 secretion. Use of the nitric oxide releaser DETA/NO (2,2'-(hydroxynitrosohydrazono)bis-ethanimine) demonstrated that nitric oxide does not inhibit interleukin-1beta induced interleukin-6 secretion. These results demonstrated that nitric oxide has no influence on interleukin-1beta induced interleukin-6 secretion in cultured pituicytes. However the results are showing that interferon-gamma has an inhibitory effect on interleukin-6 secretion.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Thorn A,Tuxen M,Moesby L,Hansen EW,Christensen JDdoi
10.1016/j.ejphar.2005.06.045keywords:
subject
Has Abstractpub_date
2005-09-05 00:00:00pages
168-74issue
1-2eissn
0014-2999issn
1879-0712pii
S0014-2999(05)00720-Xjournal_volume
519pub_type
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