Abstract：
AIM:To develop an automated, high throughput extraction protocol in order to produce database eligible profiles from fingerprints and other low copy number (LCN) DNA sources. METHODS:Extraction of either purified control DNA or buccal cells, for example, with commercial kits was compared to extraction with a simple digestion buffer and a subsequent concentration and purification. Results were evaluated based on the amount of DNA recovered and the completeness of the DNA profiles produced. RESULTS:Simple procedures with fewer steps were superior to commercial kits, such as DNA IQ (Promega, Madison, WI, USA) and QiaAmp (Qiagen, Valencia, CA, USA), and other protocols with many manipulations. The optimized protocol included a thirty-minute incubation with 0.01% SDS and proteinase K at 56 degrees C, followed by an incubation at 100 degrees C for 10 minutes. Concentration of the extract and removal of the SDS was accomplished with a Microcon 100 (Millipore, Bedford, MA, USA), which can be assembled into a 96 well plate, the Microcon-96 Retentate Assembly Plate (Millipore) for automation. The addition of 1 ng Poly A RNA to the Microcon significantly improved DNA recovery. CONCLUSION:A one-step sample digestion followed by sample concentration/purification minimized sample loss and maximized amplification input. Moreover, this methodology can be easily adapted for automation. Implementation of this protocol, due to the numerous potential sources of LCN DNA samples, will enhance the recovery of biological evidence from crime scenes and may be a source of database profiles.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Schiffner LA,Bajda EJ,Prinz M,Sebestyen J,Shaler R,Caragine TAkeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
578-86issue
4eissn
0353-9504issn
1332-8166journal_volume
46pub_type
杂志文章abstract：AIM:To evaluate the effectiveness of suture anchors in the reconstruction of chronic collateral ligaments of fingers. METHODS:We treated 8 patients, 6 with chronic instability of the collateral ligament of the thumb and 2 with the instability of the fifth finger, using the Statak suture anchor. A stable joint was achi...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：AIM:To establish the incidence of tumors of the central nervous system (CNS) in the population of Labin area in Istria, Croatia, characterized by very little population migration. METHOD:We retrospectively analyzed data on 175 patients (102 men and 73 women) diagnosed with CNS tumor according to the World Health Organ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：:On behalf of the European Commission, a Cross-Border Health Care Project was undertaken to explore how citizens living in the Euregio Meuse-Rhine can obtain improved access to health services in the Member States concerned: Belgium, Germany, and The Netherlands. Main attention of the project is focused on practical is...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：AIM:To update the estimate of multiple sclerosis (MS) prevalence in Croatia using multiple epidemiological tools. METHODS:This level IV, epidemiological study gathered data from three national patient registries and one database of a non-governmental MS patients' organization. Data were extracted on all individuals wh...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2018.59.65
更新日期：2018-04-30 00:00:00
abstract：:To examine ways of ensuring access to health services within the framework of primary health care (PHC), since the goal of PHC to make universal health care available to all people has become increasingly neglected amid emerging themes of globalization, trade, and foreign policy. From a public health point of view, we...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/2010.51.181
更新日期：2010-06-01 00:00:00
abstract：:The Revised European-American Classification of Lymphoid Neoplasms (REAL) classification has been validated by a multi-institutional study, and project data showed that it is both reproducible and clinically relevant. The new World Health Organization (WHO) Classification of Neoplastic Diseases of Hematopoietic and Ly...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：AIM:To identify and characterize the subunit structure of interleukin-13 receptor (IL-13R) in human medulloblastoma cell lines and target them with a chimeric fusion protein consisting of interleukin-13 and Pseudomonas exotoxin (termed as IL-13 cytotoxin). METHODS:Five human medulloblastoma cell lines were examined fo...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：AIM:To compare morbidity and mortality of patients with severe intra-abdominal infections after two types of surgical treatment, on-demand ("wait and see") relaparotomy and modified planned relaparotomy. METHODS:We prospectively analyzed the outcomes of 65 patients with severe peritonitis surgically treated in two Cro...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：AIM:To determine types and frequency of CAPN3 mutations in 29 unrelated Croatian families, analyzed during 6-year prospective and ongoing genetic and epidemiological study of muscular dystrophies in Croatia. METHODS:Mutation analysis included allele-specific polymerase chain reaction (PCR) or combination of PCR and re...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-08-01 00:00:00
abstract：AIM:To evaluate the accuracy of eye color prediction based on six IrisPlex single nucleotide polymorphisms (SNP) in a Slovenian population sample. METHODS:Six IrisPlex predictor SNPs (HERC2 - rs12913832, OCA2 - rs1800407, SLC45A2 - rs16891982 and TYR - rs1393350, SLC24A4 - rs12896399, and IRF4 - rs12203592) of 105 ind...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2013.54.381
更新日期：2013-08-01 00:00:00
abstract：AIM:To evaluate successes in the clinical management of severe diarrheal diseases in a busy referral hospital in Ghana, four years after the introduction of the World Health Organization's protocol for the clinical management of diarrhea and the establishment of an oral rehydration therapy Corner. METHOD:Data on the c...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：AIM:To test antinociceptive properties of botulinum toxin type A (BTX-A) in rats with carrageenan- and capsaicin-induced pain and inflammation. METHODS:Pain was provoked with carrageenan (1%) or capsaicin (0.1%) injection into the plantar surface of the rat paw-pad. The effect of BTX-A 5 U/kg on carrageenan- and capsa...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：:The Croatian Pharmaceutical Sector Reform Project was one component of a larger Health Reform Project financed by the World Bank. The Croatian government was concerned that Croatia appeared to spend more money on medicinal drugs than most other countries in the region; that the price of drugs purchased in Croatia was ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：AIM:To asses prevalence of essential arterial hypertension in family members of soldiers killed in 1992-1995 war in Bosnia and Herzegovina. METHODS:The study enrolled 1144 subjects who lost a family member in the war and 582 of their close neighbors who experienced no such loss. Data on their medical history and habit...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：AIM:To examine the usefulness of molecular analysis of IgH gene rearrangement in assessment of clonality in bone marrow biopsies with lymphoid aggregates (LA) and/or nodular lymphoid hyperplasia (NLH) in patients with different subtypes of malignant lymphomas. METHOD:Five hundred and twenty nine samples of bone marrow...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:Polish physicians-philosophers tried to find a compromise between medicine as a science and medicine as a healing art. They stated that clinical practice should be transformed into science, bearing in mind that there would be no medicine without the existence of the sick. A perfect physician is a good and wise person ...
journal_title：Croatian medical journal
pub_type： 历史文章,杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：AIM:To estimate the incidence and mortality trends of gastric and colorectal cancers in Croatia between 1988 and 2008. METHODS:Incidence data for the period 1988-2008 were obtained from the Croatian National Cancer Registry. The number of deaths from gastric and colorectal cancers were obtained from the World Health O...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2012.53.124
更新日期：2012-04-01 00:00:00
abstract：:Academic medicine is usually considered to be a tripartite composition of teaching, research, and health care services delivery or patient care. The meaning of each component varies with interpretation. Developing countries such as Malawi face particular challenges in the field of academic medicine, including research...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：AIM:To examine relationships among combat exposure, posttraumatic stress disorder (PTSD) symptoms, depression, suicidality, nicotine dependence, and religiosity in Croatian veterans. METHODS:This cross-sectional study used Combat Exposure Scale (CES) to quantify the stressor severity, PTSD Checklist 5 (PCL) to quantif...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2018-08-31 00:00:00
abstract：AIM:To compare the health-related quality of life of unemployed and employed women with disabilities and establish factors affecting their life satisfaction. METHODS:The study included 318 women with disabilities, 160 of whom were employed and 158 unemployed, paired according to age and region of residence. The health...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.550
更新日期：2011-08-15 00:00:00
abstract：AIM:To compare the quality of the 2008 cancer mortality data of the Istanbul Directorate of Cemeteries (IDC) with the 2008 data of International Agency for Research on Cancer (IARC) and Turkish Statistical Institute (TUIK), and discuss the suitability of using this databank for estimations of cancer mortality in the fu...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2012.53.480
更新日期：2012-10-01 00:00:00
abstract：AIM:To assess diseases outcomes and tolerability of real-life second-line nivolumab in a series of metastatic renal cell carcinoma (mRCC) patients. METHODS:This retrospective chart review involved prospectively monitored patients (named patient program) treated with second-line nivolumab for mRCC at the University Hos...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2020-08-31 00:00:00
abstract：AIM:To evaluate maternal and paternal smoking habits during pregnancy and determine their correlation with pregnancy complications and newborn status. METHODS:The study included 499 pregnant women who delivered at the Department of Obstetrics and Gynecology in Uludag University School of Medicine, over a period of one...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：AIM:To immunohistochemically evaluate the expression of MAGE-A1, MAGE-A, and NY-ESO-1 cancer/testis (C/T) tumor antigens in medullary breast cancer (MBC) tumor samples and to analyze it in relation to the clinicopathological features. METHODS:This retrospective study included samples from 49 patients: 40 with typical ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.171
更新日期：2011-04-15 00:00:00
abstract：AIM:To evaluate the administration of antipyretics to children with upper respiratory tract infections (URTI) by their parents or guardians without consultation with physicians, and compare epidemiological and clinical characteristics of patients who received antipyretics and of untreated patients. METHODS:A prospecti...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.48
更新日期：2011-02-01 00:00:00
abstract：:Aim .To investigate if there are differences in medical ethics education between different schools of medicine in Spain, specifically between private and public schools and between recently founded schools and older ones. METHOD:The curricula of medical degrees from all Spanish faculties were reviewed for the 2014/20...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.493
更新日期：2016-10-31 00:00:00
abstract：AIM:To assess the effectiveness of the long-term group psychotherapy in the treatment of posttraumatic stress disorder (PTSD) in war veterans on the basis of clinical picture of PTSD, associated neurotic symptoms, and adopted models of psychological defense mechanisms. METHODS:Prospective cohort study involved 59 war ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-02-01 00:00:00
abstract：AIM:To compare the early biochemical response and rate of adverse effects in patients who received prednisolone (PRED)/azathioprine (AZA) and those who received budesonide (BUD)/AZA as the first-line treatment for autoimmune hepatitis. METHODS:The study involved 25 patients receiving PRED 30 mg/day + AZA 50 mg/day and...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2019-08-31 00:00:00
abstract：AIM:To compare the growth patterns of Qatari preschool children aged 0-5 years with the growth reference charts developed by the American National Center for Health Statistics and Centers for Disease Control and Prevention (NCHS/CDC). METHODS:The demographic data of weight, height, and age were collected in a cross-se...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-08-01 00:00:00
abstract：:The aim of this article is to offer a concise interpretation of the scientific data about the topic of Croatian genetic heritage that was obtained over the past 10 years. We made a short overview of previously published articles by our and other groups, based mostly on Y-chromosome results. The data demonstrate that C...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.225
更新日期：2011-06-01 00:00:00
