Abstract:
AIM:To identify and characterize the subunit structure of interleukin-13 receptor (IL-13R) in human medulloblastoma cell lines and target them with a chimeric fusion protein consisting of interleukin-13 and Pseudomonas exotoxin (termed as IL-13 cytotoxin). METHODS:Five human medulloblastoma cell lines were examined for the expression of IL-13R subunits at the mRNA and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR) and indirect immunofluorescence studies, respectively. In addition, IL-13 cytotoxin-induced cytotoxicity was examined in these medulloblastoma cell lines by measuring protein synthesis inhibition. RESULTS:All five medulloblastoma cell lines expressed mRNA and proteins for IL-4Ra and IL-13Ra1 chains, whereas three of the cell lines also showed the presence of IL-13Ra2. mRNA or protein for IL-2gc chain was not detected in any of the cell lines. Consistent with the expression of IL-13Ra2 chain, IL-13 cytotoxin was highly and specifically cytotoxic to three of five medulloblastoma cell lines. The sensitivity of medulloblastoma cell lines to IL-13 cytotoxin could be completely eliminated by concurrent incubation with excess of IL-13, but not with IL-2 or IL-4. CONCLUSION:These studies establish IL-13R, in particular IL-13Ra2, as a medulloblastoma-associated target for IL-13 cytotoxin therapy. IL-13 cytotoxin may be useful for medulloblastoma therapy. Alternatively, IL-13Ra2 may serve as a tumor-specific antigen for active immunotherapy.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Joshi BH,Leland P,Puri RKkeywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
455-62issue
4eissn
0353-9504issn
1332-8166journal_volume
44pub_type
杂志文章abstract:AIM:In an effort to help alleviate the lack of an adequate pediatric cardiac surgical service that existed in Croatia following 1991 the International Childrens Heart Foundation (ICHF) was asked to provide the necessary surgery. Initially, this project was undertaken as a humanitarian program to provide pediatric cardi...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract:AIM:To investigate the influence of 1991-1995 war on the prevalence, duration, and practice of breast-feeding Croatian children up to 5 years of age. METHOD:In 1996, interviews were conducted in households with children up to 2 years of age (757 children) and 2-5 years of age (1,180 children). Data for war-free areas,...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Corporate medical practice, a market economy, and a consumer culture are transforming health care. The service relationships of doctors with patients are now commodities. The doctor, directed by disease management protocols (to improve outcomes, reduce costs, and standardize care), is, in effect, providing programmed ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract:AIM:To asses prevalence of essential arterial hypertension in family members of soldiers killed in 1992-1995 war in Bosnia and Herzegovina. METHODS:The study enrolled 1144 subjects who lost a family member in the war and 582 of their close neighbors who experienced no such loss. Data on their medical history and habit...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract:AIM:To determine types and frequency of CAPN3 mutations in 29 unrelated Croatian families, analyzed during 6-year prospective and ongoing genetic and epidemiological study of muscular dystrophies in Croatia. METHODS:Mutation analysis included allele-specific polymerase chain reaction (PCR) or combination of PCR and re...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract:AIM:To compare the myocardial effects of cardioplegia by warm blood, tepid blood, and cold crystalloid during coronary artery bypass grafting (CABG). METHODS:Patients undergoing CABG surgery at Kaunas University Hospital between 2000 and 2004 were randomized into three groups (n=156), receiving a different method of c...
journal_title:Croatian medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2005-12-01 00:00:00
abstract:AIM:To determine changes in sexual behaviors and other relevant characteristics related to human immunodeficiency virus (HIV) and sexually transmitted infection (STI) risks among young Croatian adults. METHOD:We surveyed adults aged 18-24 in 2005 (n=1092) and 18-25 in 2010 (n=1005). Both samples were probabilistic and...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.458
更新日期:2011-08-15 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2007-02-01 00:00:00
abstract:AIM:To investigate whether socioeconomic inequalities at a micro-scale, through their effect on major health risk factors and other health indicators, contribute to health status in an isolated island population with demonstrated reduced genetic and environmental variability. METHODS:This cross-sectional study was per...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2010.51.259
更新日期:2010-06-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2012.53.301
更新日期:2012-08-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2016.57.558
更新日期:2016-12-31 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2017.58.384
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2018.59.65
更新日期:2018-04-30 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2014.55.672
更新日期:2014-12-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
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journal_title:Croatian medical journal
pub_type: 杂志文章
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2012.53.480
更新日期:2012-10-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2008.1.75
更新日期:2008-02-01 00:00:00
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journal_title:Croatian medical journal
pub_type:
doi:
更新日期:2019-10-31 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract::The Faculty of Medicine in Tirana, the only institution providing medical training in Albania, has currently developed three separate branches of study, General Medicine, Dentistry, and Pharmacy. Since the early 1990s, following the collapse of the communist regime, many faculty members have been trained abroad in dif...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
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journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00