Abstract:
:The in vitro sponge-gel-supported three-dimensional histoculture chemosensitivity assay (Hoffman assay) allows the in vivo-like culture of human tumors. In this study, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end point was applied to the Hoffman assay in an attempt to increase in vitro-in vivo correlation. The chemosensitivities of 16 human tumor lines were determined in vitro by the histoculture assay, and retrospectively correlated to their in vivo chemosensitivity as xenografts in nude mice. The in vitro test was considered to be positive if tumor-cell MTT reduction activity was lowered by more than 50%. The cutoff drug concentrations to determine sensitivity in vitro were determined for mitomycin C, doxorubicin, 5-fluorouracil and cisplatin. Using these cutoff drug concentrations in vitro we found, as a function of time of exposure, a strong correlation between serum drug concentrations found in nude mice given maximum tolerated doses and drug concentrations found in the histoculture media in vitro, thereby establishing a relationship between the amounts of drugs to which tumors were exposed in vivo and in vitro. The overall correlation rate of the efficacy results of the drug-response assay to in vivo chemosensitivities was 89.8%, with 90.0% true-positive and 89.7% true-negative rates, 81.7% sensitivity and 94.6% specificity, thereby indicating potential clinical use for tumor histoculture with the MTT end point.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Furukawa T,Kubota T,Watanabe M,Takahara T,Yamaguchi H,Takeuchi T,Kase S,Kodaira S,Ishibiki K,Kitajima Mdoi
10.1002/ijc.2910510325keywords:
subject
Has Abstract,Author List Incompletepub_date
1992-05-28 00:00:00pages
489-98issue
3eissn
0020-7136issn
1097-0215journal_volume
51pub_type
杂志文章abstract::Nitrogen-containing bisphosphonates (N-BPs) are effective antiosteolytic agents in patients with multiple myeloma. Preclinical studies have also demonstrated that these agents have direct antitumor effects in vitro and can reduce tumor burden in a variety of animal models, although it is not clear whether such effects...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24758
更新日期:2010-01-01 00:00:00
abstract::While platelet-derived growth factor, PDGF, is not regularly expressed in mesenchymal tissues, PDGF B mRNA is often found in tumors derived from these tissues. PDGF B protein is also present, but the protein levels in individual tumors do not appear to correlate well with those of the mRNA. PDGF B is homologous to the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-01-15 00:00:00
abstract::The relationship between renal-cell cancer (RCC) and occupation was investigated in an international multicenter population-based case-control study. Study centers in Australia, Denmark, Germany, Sweden and the United States interviewed 1732 incident RCC cases and 2309 controls. Significant associations were found wit...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910610503
更新日期:1995-05-29 00:00:00
abstract::Patients with head-and-neck cancer commonly have immune defects. It was reported that these patients have raised serum IgA levels. We investigated whether IgA-anti-Fab autoantibodies, which occur in association with immune dysfunction, are present in patients with head-and-neck cancer. Sera of 101 patients with squamo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570109
更新日期:1994-04-01 00:00:00
abstract::Environmental carcinogens often induce specific mutations in the p53 gene, apparent in tumors. The relation between aflatoxin B1 (AFB1 )-related hepatocellular carcinomas (HCCs) and hot spot at codon 249 of the p53 gene has received a great deal of attention, but its significance is still controversial. To clarify thi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990719)82:2<187::aid-ijc
更新日期:1999-07-19 00:00:00
abstract::Treatment of lung cancer is often performed with cone-down oblique beams to spare spinal cord and normal structures. However, there is no optimum technique to determine oblique beam angles when a CT simulation is not available. Impact of oblique beam angle was investigated in this study. Fifteen patients with centrall...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20001220)90:6<359::aid-ijc8>3.0.
更新日期:2000-12-20 00:00:00
abstract::Cytoskeleton and extracellular matrix-related proteins (CECMPs) represent the most common class of cancer mutants, owing to the large size of their coding regions and the randomness of mutagenesis. We used a bioinformatics approach to assess the impact of amino acid (AA) substitutions on the sensitivity of CECMPs to p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31111
更新日期:2018-03-01 00:00:00
abstract::Epidemiologic evidence on the association of antioxidant intake and prostate cancer incidence is inconsistent. Total antioxidant intake and prostate cancer incidence have not previously been examined. Using the ferric-reducing antioxidant potential (FRAP) assay, the total antioxidant content (TAC) of diet and suppleme...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28438
更新日期:2014-03-01 00:00:00
abstract::It has been suggested that phytoestrogens protect against hormone-dependent cancers. Lignans are the main class of phytoestrogens in Western diets. We conducted a prospective study of endometrial cancer and circulating levels of the main human lignan, enterolactone. The design was a case-control study nested within 3 ...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.22140
更新日期:2006-11-15 00:00:00
abstract::We describe the anatomic and histologic presentation and prognosis of non-Hodgkin's lymphoma (NHL) among people with AIDS (PWA) and determine their contribution to the NHL burden. We linked AIDS and cancer registries in selected areas of the United States and compared NHL sites and histologies in PWA and non-PWA, afte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19971127)73:5<645::aid-ijc
更新日期:1997-11-27 00:00:00
abstract::Apigenin is a plant flavonoid that is thought to play a role in the prevention of carcinogenesis. However, its mechanism of action has not yet been elucidated. Because of the importance of angiogenesis in tumor growth, we investigated the effect of apigenin on endothelial and smooth-muscle cells in an in vitro model. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000301)85:5<691::aid-ijc
更新日期:2000-03-01 00:00:00
abstract::Familial risk of pancreatic cancer has been mainly assessed through case-control studies based on reported but not medically verified cancers in family members. We used the nationwide Swedish Family-Cancer Database on 10.2 million individuals and 21,000 pancreatic cancers to calculate standardized incidence ratios (SI...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10863
更新日期:2003-02-10 00:00:00
abstract::Regulatory T cells (Treg) mediate amelioration of disease and immune homeostasis by inhibiting immune activation and maintaining peripheral immune tolerance. The suppressive mechanisms and clinical significance of Treg have not been completely elucidated in patients with acute myeloid leukemia (AML). Here, we demonstr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25791
更新日期:2011-09-15 00:00:00
abstract::Although new treatments have been widely studied to improve the survival of patients with metastatic breast cancer (BC), prognosis continues to be poor with an average survival time no longer than 3 years. We carried on a population-based study with the purpose of evaluating the outcome of metastatic breast cancer in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29503
更新日期:2015-09-15 00:00:00
abstract::Tumor cells interact with endothelial cells during both intra- and extravasation. Understanding how these interactions are modulated could lead to the development of ways to alter the metastatic potential of tumor cells. Three pancreatic cancer cell lines, SW1990, CAPAN-2 and PANC-I, were examined for their ability to...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570621
更新日期:1994-06-15 00:00:00
abstract::Since it is difficult to predict the probability of persistent teratoma or of a viable tumor in patients with normalized tumor markers and a normal CT scan following chemotherapy for advanced stage testis cancer, recommendations regarding adjunctive surgery have ranged from observation to surgical exploration for all ...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/(sici)1097-0215(19991210)83:6<852::aid-ijc
更新日期:1999-12-10 00:00:00
abstract::MET is a master gene controlling a genetic program driving proliferation, apoptosis protection and invasion. The ROR1 pseudokinase acts as a MET substrate. However, its contribution to MET signaling and MET-dependent biological outcomes remains to be elucidated. By structure-function analysis of ROR1 mutants, we show ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28879
更新日期:2014-11-15 00:00:00
abstract::The stability of a cloned murine tumor for sensitivity to NR was examined following growth in vivo in order to test the hypothesis that tumor progression proceeds through the generation and selection of variants. Clonal sensitivity to the [131I]-dUrd elimination assay of NR was assessed for the L5178Y-F9 tumor grown i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910310116
更新日期:1983-01-15 00:00:00
abstract::Adenoviral oncolysis is a promising new modality for treatment of cancer based on selective viral replication in tumor cells. However, tumor cell killing by adenoviral oncolysis needs to be improved to achieve therapeutic benefit in the clinic. Towards this end, the activation of anti-tumor immunity by adenoviral onco...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23074
更新日期:2008-01-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity, representing 90% of all oral carcinomas and accounting for 3-5% of all malignancies. The WWOX gene (WW-domain containing oxidoreductase) is a candidate tumor suppressor gene located at 16q23.3-24.1, spanning the second most c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21446
更新日期:2006-03-01 00:00:00
abstract::Epigenetic alterations play an important role in carcinogenesis. Recent studies suggested that global histone modifications are predictors of cancer recurrence in various tumor entities. Our study was performed to evaluate histone H3 lysine 4 mono-methyl (H3K4me1), -di-methyl (H3K4me2) and -trimethyl (H3K4me3) pattern...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25250
更新日期:2010-11-15 00:00:00
abstract::Classical midgut carcinoids are rare intestinal neuroendocrine tumors that often present with metastases at diagnosis. In contrast to foregut carcinoids, midgut carcinoids are not related to the multiple endocrine neoplasia type 1 syndrome, and the mechanisms involved in their tumorigenesis are unknown. Eight classica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1276
更新日期:2001-06-15 00:00:00
abstract::MAGE genes encoding tumor-rejection antigens recognized by cytotoxic T lymphocytes are expressed at the mRNA level in various malignant tumors. We have investigated the expression of genes MAGE-1, -2, -3/-6 and -4a/-4b at the mRNA level in malignant and non-malignant ovarian tumors as well as in normal ovaries by reve...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640607
更新日期:1995-12-20 00:00:00
abstract::Gene transfer is an attractive approach to fight cancer by targeting cancer cells or their vasculature. Our study reports the inhibition of tumor growth and angiogenesis by a nonviral method using dendrimers associated with 36-mer anionic oligomers (ON36) for delivering angiostatin (Kringle 1-3) and tissue inhibitor o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11105
更新日期:2003-06-20 00:00:00
abstract::Increased levels of hypoxia and hypoxia-inducible factor 1α (HIF-1α) in human sarcomas correlate with tumor progression and radiation resistance. Prolonged antiangiogenic therapy of tumors not only delays tumor growth but may also increase hypoxia and HIF-1α activity. In our recent clinical trial, treatment with the v...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27666
更新日期:2013-01-01 00:00:00
abstract::Ninety-eight cases of transitional-cell carcinoma (TCC) of the renal pelvis and ureter, including dysplastic lesions, were studied for tumor incorporation of human papillomavirus (HPV) type-16 and type-18 DNA by in situ hybridization (ISH) with DNA probes for each HPV viral type. Immunohistochemical analysis of p53 ex...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640503
更新日期:1995-10-20 00:00:00
abstract::Loss of heterozygosity (LOH) on chromosome 13 and the age of patients at operation were studied in 46 cases of retinoblastoma (RB) tumors, of which 25 were hereditary and 21 were non-hereditary. The frequency of LOH was 70% for all informative tumors, but significantly higher in non-hereditary tumors (90%) than in her...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540609
更新日期:1993-07-30 00:00:00
abstract::Although there are several hypotheses explaining the mechanisms of immune-privileged status of malignant tumor, the exact pathway has yet to be explored. Cyclooxygenase (COX)-2 plays a vital role in prognosis of cancer patients in terms of contribution to neoangiogenesis and apoptosis inhibition; however, the impact o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20777
更新日期:2005-05-01 00:00:00
abstract::Cytokines such as Interleukin (IL)-12p70 ("IL-12") and IL-23 can influence tumor progression. We tested the hypothesis that blood levels of IL-12p40, the common subunit of both cytokines, are associated with melanoma progression. Blood from 2,048 white melanoma patients were collected at a single institution between M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29182
更新日期:2015-04-15 00:00:00
abstract::Changes in the length of a polymorphic trinucleotide (CAG) repeat in the androgen receptor (AR) gene, which may lead to altered transactivation of the AR gene, have been implicated to play a role in the pathogenesis of several forms of endocrine cancer and certain reproductive disorders. Subjects with reproductive dis...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10680
更新日期:2002-11-10 00:00:00