Abstract:
:Synovial fluid from patients with various arthritides contains procoagulant, cell-derived microparticles. Here we studied whether synovial microparticles modulate the release of chemokines and cytokines by fibroblast-like synoviocytes (FLS). Microparticles, isolated from the synovial fluid of rheumatoid arthritis (RA) and arthritis control (AC) patients (n = 8 and n = 3, respectively), were identified and quantified by flow cytometry. Simultaneously, arthroscopically guided synovial biopsies were taken from the same knee joint as the synovial fluid. FLS were isolated, cultured, and incubated for 24 hours in the absence or presence of autologous microparticles. Subsequently, cell-free culture supernatants were collected and concentrations of monocyte chemoattractant protein-1 (MCP-1), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF) and intracellular adhesion molecule-1 (ICAM-1) were determined. Results were consistent with previous observations: synovial fluid from all RA as well as AC patients contained microparticles of monocytic and granulocytic origin. Incubation with autologous microparticles increased the levels of MCP-1, IL-8 and RANTES in 6 of 11 cultures of FLS, and IL-6, ICAM-1 and VEGF in 10 cultures. Total numbers of microparticles were correlated with the IL-8 (r = 0.91, P < 0.0001) and MCP-1 concentrations (r = 0.81, P < 0.0001), as did the numbers of granulocyte-derived microparticles (r = 0.89, P < 0.0001 and r = 0.93, P < 0.0001, respectively). In contrast, GM-CSF levels were decreased. These results demonstrate that microparticles might modulate the release of chemokines and cytokines by FLS and might therefore have a function in synovial inflammation and angiogenesis.
journal_name
Arthritis Res Therjournal_title
Arthritis research & therapyauthors
Berckmans RJ,Nieuwland R,Kraan MC,Schaap MC,Pots D,Smeets TJ,Sturk A,Tak PPdoi
10.1186/ar1706keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
R536-44issue
3eissn
1478-6354issn
1478-6362pii
ar1706journal_volume
7pub_type
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journal_title:Arthritis research & therapy
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pub_type: 杂志文章,多中心研究
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journal_title:Arthritis research & therapy
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journal_title:Arthritis research & therapy
pub_type: 杂志文章,评审
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pub_type: 杂志文章,随机对照试验
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doi:10.1186/ar4609
更新日期:2014-07-10 00:00:00
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pub_type: 杂志文章
doi:10.1186/ar1882
更新日期:2006-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 评论,杂志文章,评审
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doi:10.1186/ar3057
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pub_type: 社论
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-11-06 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:Arthritis research & therapy
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更新日期:2005-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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更新日期:2014-01-31 00:00:00
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pub_type: 杂志文章
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