Abstract:
:Metaphase spindles assemble to a steady state in length by mechanisms that involve microtubule dynamics and motor proteins, but they are incompletely understood. We found that Xenopus extract spindles recapitulate the length of egg meiosis II spindles, by using mechanisms intrinsic to the spindle. To probe these mechanisms, we perturbed microtubule polymerization dynamics and opposed motor proteins and measured effects on spindle morphology and dynamics. Microtubules were stabilized by hexylene glycol and inhibition of the catastrophe factor mitotic centromere-associated kinesin (MCAK) (a kinesin 13, previously called XKCM) and destabilized by depolymerizing drugs. The opposed motors Eg5 and dynein were inhibited separately and together. Our results are consistent with important roles for polymerization dynamics in regulating spindle length, and for opposed motors in regulating the relative stability of bipolar versus monopolar organization. The response to microtubule destabilization suggests that an unidentified tensile element acts in parallel with these conventional factors, generating spindle shortening force.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Mitchison TJ,Maddox P,Gaetz J,Groen A,Shirasu M,Desai A,Salmon ED,Kapoor TMdoi
10.1091/mbc.e05-02-0174keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
3064-76issue
6eissn
1059-1524issn
1939-4586pii
E05-02-0174journal_volume
16pub_type
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