Abstract:
BACKGROUND:Asparagus acutifolius L. is a dioecious and native plant species, widely distributed in the Mediterranean Basin. It is known for its fine flavour and could represent an important resource for cultivation programs in desert areas. Few molecular studies have been performed on this species. In the present paper, the ISSR technique was employed to study genetic diversity in Italian A. acutifolius. RESULTS:Twenty-three primers produced a total of 228 polymorphic fragments used to evaluate genetic variation. FST (0.4561) and Theta B (0.4776) values indicate a wide genetic variation among the samples examined. The distance UPGMA tree grouped together the genotypes strictly according to their geographical origin, showing that each sample is genetically structured and can be considered a distinct population. AMOVA analysis further confirmed genetic structuring of the populations. Population-specific fragments were also detected. CONCLUSION:The results suggest that ISSR markers are useful in distinguishing the populations of A. acutifolius according to geographical origin, and confirm the importance of genetic studies for designing germplasm conservation strategies.
journal_name
BMC Genetjournal_title
BMC geneticsauthors
Sica M,Gamba G,Montieri S,Gaudio L,Aceto Sdoi
10.1186/1471-2156-6-17keywords:
subject
Has Abstractpub_date
2005-03-18 00:00:00pages
17issn
1471-2156pii
1471-2156-6-17journal_volume
6pub_type
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