Abstract:
:Some 25%-70% of sporadic medullary thyroid carcinomas (MTCs) are associated with somatic mutations within the RET proto-oncogene. In a significant number of MTCs, however, no such genetic variations can be detected, which implies alternative pathogenic molecular alterations. To assess altered RET mutation-specific gene expression, and to identify yet unknown gene transcripts involved in the tumorigenesis of MTC, we performed an expression analysis by mRNA differential display (RT-DD). Snap-frozen tumor tissues and corresponding normal thyroid tissues of 8 patients suffering from MTC (6 sporadic, 2 hereditary tumors) were included in the study; 5/8 MTCs harbored RET point mutations (codons 618, 634, 918). The RT-DD method was refined by use of fluorescence-labeled arbitrary oligonucleotides, electrophoresis on an automated sequencer, and a novel fragment-recovery technique utilizing a high-performance fluorescence scanner. More than 400 differentially expressed mRNA transcripts--representing upregulated or downregulated genes in the compared tissues--were detected. In all, 28 selected fragments were recovered, cloned, sequenced, and identified. Differential expression of gene transcripts with known association to cell proliferation or tumor progression--such as annexin A2, Rab11a, trefoil proteins, superoxide dismutase (SOD1), mitochondrial displacement loop (D-loop), and G protein subunit gamma11--as well as of the neuroendocrine marker chromogranin was observed. Furthermore, several mRNA transcripts of yet unknown genes displayed mutation-specific upregulation or downregulation in MTC. Illumination of the molecular basis especially of C-cell carcinomas without detectable alterations of the RET receptor tyrosine kinase will be required for the development of therapeutic strategies for advanced tumors that cannot be bridled or cured by surgical interventions alone.
journal_name
World J Surgjournal_title
World journal of surgeryauthors
Musholt TJ,Hanack J,Brehm C,von Wasielewski R,Musholt PBdoi
10.1007/s00268-004-7748-ykeywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
472-82issue
4eissn
0364-2313issn
1432-2323journal_volume
29pub_type
杂志文章abstract:BACKGROUND:Pheochromocytoma with synchronous ipsilateral adrenal cortical adenoma (PSCA) may present with mixed clinical, biochemical, and radiological features characteristic to each neoplasm subtype. METHODS:All patients with a pathological diagnosis of pheochromocytoma were evaluated for an ipsilateral cortical ade...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4110-8
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Molecular diagnostics can allow some patients with indeterminate thyroid nodule cytopathology to avoid diagnostic hemithyroidectomy; however, the testing is costly. We hypothesized that molecular testing with the intention of preventing unnecessary diagnostic hemithyroidectomy would be cost-effective if this...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05177-7
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:Ultrasound (US) and sestamibi (MIBI) are traditionally considered positive or negative. The purpose of this study was to define and test a new scoring system for MIBI and US and to determine whether this can improve their accuracy for primary hyperparathyroidism. METHODS:This is a prospective study of 200 c...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1666-1
更新日期:2012-10-01 00:00:00
abstract::Acute acalculous cholecystitis (AAC) is a well known complication in severely traumatized patients. Existing data of AAC originate from retrospective analyses and episodic case reports. In a prospective study 45 polytraumatized patients admitted to our intensive care unit from January 1989 to June 1990 were clinically...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF02067089
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:Laparoscopic intersphincteric resection (ISR) after neoadjuvant chemoradiation is helpful in the management of patients with low rectal cancer. With the advent of this technique, the need for performance of abdominoperineal resection seems to have decreased in patients with very low rectal tumors. The aim of...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-011-1277-2
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:There are scant studies that have analyzed the prognostic factors for malignant nonfunctioning endocrine tumors of the pancreas. We identified the predictive factors associated with long-term survival after surgical resection for malignant nonfunctioning endocrine tumors. METHODS:Among the 25 patients seen ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0585-4
更新日期:2007-03-01 00:00:00
abstract:INTRODUCTION:Hypoxic stress is a feature of rapidly growing thyroid tumours. Cancer progression is thought to be driven by a small population of tumour cells possessing stem cell properties. Hypoxia-inducible factors (HIFs) are important mediators of hypoxia. Both HIF-1alpha and HIF-2alpha have been reported to be expr...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4331-x
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the available resources for trauma care at health centers (HCs) and referral hospitals (RHs) in rural Cambodia and to examine whether the resources at HCs are allocated on the basis of actual need based on the referral distance and number of severely injured patients ref...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9900-6
更新日期:2009-04-01 00:00:00
abstract::The expression CA195 in serum, defined by monoclonal antibody CC3C195 (IgM), was studied in 67 patients with pancreatic cancer and in 138 patients with biliary or pancreatic benign disease. The results were compared with carcinoembryonic antigen (CEA) expression. The overall sensitivity of the CA195 assay (> 12 U/ml) ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/pl00013182
更新日期:1999-03-01 00:00:00
abstract::Chondrosarcomas of the head and neck are uncommon. Over a 35-year period (1950-1985) at our institution, 557 patients had an established diagnosis of chondrosarcoma. In 28 (5%) of these patients, the tumor was located in the head and neck region. A retrospective analysis was performed in an attempt to accurately defin...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF02067021
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND:The goal of the present study was to determine the frequency, mode of presentation, and need for reoperation in the treatment of recurrent inguinal hernia at Liaquat University Hospital, Jamshoro, Sindh, Pakistan. METHODS:This descriptive, cross-sectional study was conducted over a period of four years, fro...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1897-1
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:The aim of the study was to evaluate risk factors for small bowel obstruction (SBO) in early postoperative period after anterior resection for rectal cancer. METHODS:Patients who underwent anterior resection (AR) [high AR (HAR) or low AR (LAR)] for rectal cancer between January 2009 and April 2016 were enrolle...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4152-y
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Finnmark County is the northernmost county in Norway. For several decades, the rate of mortality after injury in this sparsely inhabited region has remained above the national average. Following documentation of this discrepancy for the period 1991-1995, improvements to the trauma system were implemented. Th...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-011-1102-y
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:The survival outcome in lung cancer patients with chronic kidney disease (CKD) has not been well evaluated. The aim of this study was to evaluate the survival outcomes following non-small cell lung cancer (NSCLC) surgery in patients with CKD as a preoperative comorbidity. METHODS:Among 671 patients who unde...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05143-3
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Transient postthyroidectomy hypocalcemia occurs in up to 30% of patients. We evaluated the effect of vitamin D deficiency on postthyroidectomy hypocalcemia. METHODS:Data were collected prospectively between January 2006 and March 2009. A total of 166 consecutive total thyroidectomies were analyzed regarding...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0872-y
更新日期:2011-02-01 00:00:00
abstract::Familial Mediterranean fever (FMF) also known as hereditary polyserositis, is an inherited disorder commonly found in Armenians, Turks, Arabs, Balkans, and Jews originating from North African countries. The diagnosis of FMF is based on clinical findings and family history, as no specific diagnostic test is yet availab...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF00348205
更新日期:1994-01-01 00:00:00
abstract::The well-known poor prognosis of proximal bile duct cancer is due to its unfortunate anatomical location and its late diagnosis. Successful tumor resection, which is considered to be optimal treatment, depends on many factors. Eighty-eight patients suffering from proximal bile duct cancer underwent surgical exploratio...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-003-6656-x
更新日期:2003-06-01 00:00:00
abstract:INTRODUCTION:The surgical repair of large irreducible scrotal hernias is, in general, technically difficult. Laparoscopic repair for these cases is controversial, although extraperitoneal and transperitoneal approaches have been described. We present a small series of patients with large irreducible groin hernias (omen...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-007-9157-5
更新日期:2007-09-01 00:00:00
abstract:INTRODUCTION:Jejunal diverticula are rare lesions, and when complications arise, they pose diagnostic difficulties. Perforation is a common complication resulting in an acute abdomen, although preoperative diagnosis is usually not possible. The "gold standard" for management for patients with complications is surgery. ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9469-0
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:The impact of time to readmission (TTR) on post-discharge mortality has not been well examined. We sought to define the impact of TTR on postoperative mortality after liver or pancreas surgery. METHODS:A retrospective cohort analysis of liver and pancreas surgical patients was conducted using 2013-2015 Medi...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-018-4766-8
更新日期:2019-01-01 00:00:00
abstract::In a retrospective study the clinical course of 40 patients with symptomatic persistent or recurrent parathyroid cancer was analyzed in order to assess the value of aggressive surgical intervention. Recurrence was diagnosed after a median period of 33 months (1-228 months). Twenty-two patients had locoregional disease...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF00353773
更新日期:1994-07-01 00:00:00
abstract::There are now excellent data that patients with stage III colon cancer or stage II and III rectal cancer live longer if they receive, respectively, systemic or regional and systemic therapy after surgery. In addition, disease-free survival, particularly freedom from the symptoms of regional recurrence, in the high ris...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF00308633
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:This is a baseline assessment of surgical capacity in the Federal Capital Territory (FCT), in preparation for the creation of a National Surgical, Obstetric, Anesthesia, and Nursing Plan. METHODS:In October 2017, all 10 of the 11 secondary hospitals in FCT that provide surgical and/or obstetric care were su...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-018-4835-z
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:It is now generally believed that regulatory T cells (Tregs) play an important role in peripheral tolerance, and a defect in Tregs is considered one of the most important factors in the induction of various kinds of autoimmune disease including ulcerative colitis (UC). Here, we examined the change in frequen...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-0301-9
更新日期:2006-04-01 00:00:00
abstract:INTRODUCTION TO THE PROBLEM:Though declining in the recent decades, high-energy musculoskeletal trauma remains a major contributor to the burden of disease in high-income countries (HICs). However, due to limitations in the available body of the literature, evaluation of this burden is challenging. The purpose of this ...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-018-4742-3
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Few details of the clinical features of hepatocellular carcinoma (HCC) developing extrahepatic recurrence after a curative resection have been published. The purpose of this study was to clarify the clinicopathologic findings of patients with HCC who experienced extrahepatic metastases. METHODS:Clinicopatho...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9613-x
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:With the progressive use of new diagnostic techniques, the management of penetrating abdominal stab wounds is changing. Most studies have been conducted in well-equipped trauma centers in developed countries, and there is a paucity of reports from general teaching hospitals with limited resources. We reviewe...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-013-1931-y
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:Total thyroidectomy is a well-established surgical approach for the management of papillary thyroid cancer (PTC). However, the best surgical approach for papillary microcarcinoma is nowadays still debated. Both total thyroidectomy and simple lobectomy are used. We report the experience of a single University...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-015-3327-7
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVE:The present study was designed to evaluate functional outcome of perineal repair with and without levatorplasty versus transanal repair of rectocele in obstructed defecation. METHODS AND PATIENTS:A total of 48 multiparous women with obstructed defecation caused by a rectocele were randomly allocated to three...
journal_title:World journal of surgery
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00268-010-0390-y
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:The stapled hemorrhoidopexy is reported to have a low recurrence while treating the major hemorrhoidal symptoms of bleeding and prolapse. The aim of this study is to obtain long-term results on the outcome of the stapled hemorrhoidopexy. METHODS:All patients with a hemorrhoidal disease grade III, who underw...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05065-0
更新日期:2019-10-01 00:00:00