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A possible association between the presence of interleukin-4-secreting cells and a reduction in the risk of acute graft-versus-host disease.

Abstract：

OBJECTIVE:We monitored cytokine-secreting cells using an enzyme-linked immunospot (ELISPOT) assay in a prospective study to assess the cytokine network after transplantation. PATIENTS AND METHODS:Peripheral blood mononuclear cells were collected from 23 patients who received allogeneic stem cell transplantation, from before the preconditioning regimen to 56 days after transplantation. The number of interleukin-4 (IL-4), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha)-secreting cells were measured by ELISPOT assay. For IL-4 and IFN-gamma, in vitro stimulation with phorbol 12-myristate 13-acetate and phytohemagglutinin was performed. RESULTS:The frequency of IL-4-secreting cells was significantly higher in five patients receiving peripheral blood stem cell transplantation (PBSCT) than that in 18 patients who received bone marrow transplantation (BMT). Based on IFN-gamma and TNF-alpha release, there was a trend toward a decrease in the number of cytokine-secreting cells in PBSCT compared with BMT. Furthermore, patients who did not develop acute graft-vs-host disease (GVHD, n=5) showed a significantly higher number of IL-4-secreting cells compared with those who developed acute GVHD (n=18). Both IFN-gamma-secreting cells and TNF-alpha-secreting cells showed a trend to increase in number in patients with acute GVHD. In patients who received reduced-intensity stem cell transplantation (n=7) compared with conventional stem cell transplantation (n=16), there was a large number of cytokine-secreting cells detected by IL-4 and IFN-gamma release. CONCLUSIONS:These results are consistent with the hypothesis that IL-4-producing cells inhibit the development of acute GVHD. In addition, the increased percentage of IL-4-secreting cells may be responsible for the unexpected low incidence of acute GVHD in PBSCT, despite the presence of large numbers of mature T cells in the donor infusion.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Takabayashi M,Kanamori H,Takasaki H,Yamaji S,Koharazawa H,Taguchi J,Tomita N,Fujimaki K,Fujisawa S,Maruta A,Ishigatsubo Y

doi

10.1016/j.exphem.2004.10.009

keywords:

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

251-7

issue

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(04)00358-3

journal_volume

pub_type

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A possible association between the presence of interleukin-4-secreting cells and a reduction in the risk of acute graft-versus-host disease.