Abstract：
:We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Kim AP,Yellen P,Yun YH,Azeloglu E,Chen Wdoi
10.1016/j.biomaterials.2004.05.015keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
1585-93issue
13eissn
0142-9612issn
1878-5905pii
S0142961204004910journal_volume
26pub_type
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