Preferential production of latent transforming growth factor beta-2 by primary prostatic epithelial cells and its activation by prostate-specific antigen.


:Three mammalian isoforms of transforming growth factor-beta (TGFbeta) are known, TGFbeta1, 2, and 3, that have non-overlapping functions during development. However, their specific roles in cancers such as prostate cancer are less clear. Here we show that primary cultures of prostatic epithelial cells preferentially produce and activate the latent TGFbeta2 isoform. Paired cultures of normal and malignant prostate cells from prostate cancer patients produced predominantly the TGFbeta2 isoform, with 30- to 70-fold less TGFbeta1. By mono-Q ion exchange chromatography, three major peaks of latent TGFbeta2 activity were observed corresponding to the known small latent TGFbeta2 complex, the known large latent TGFbeta2 complex and a novel eluting peak of latent TGFbeta2. Although prostate cells are known to activate latent TGFbeta, the mechanism for activation is currently unclear. We investigated whether prostate specific antigen (PSA), a serine protease used as a clinical marker for prostate cancer, could play a role in the activation of latent TGFbeta. Unlike plasmin, a known activator of both latent TGFbeta1 and 2, PSA specifically activated the recombinant small latent form of TGFbeta2, but not TGFbeta1. Prostate epithelial cells, therefore, preferentially produce the TGFbeta2 isoform and PSA, a protease produced by the prostate, specifically targets the activation of this TGFbeta isoform. PSA-mediated activation of latent TGFbeta2 may be an important mechanism for autocrine TGFbeta regulation in the prostate and may potentially contribute to the formation of osteoblastic lesions in bone metastatic prostate cancer.


J Cell Physiol


Dallas SL,Zhao S,Cramer SD,Chen Z,Peehl DM,Bonewald LF





Has Abstract


2005-02-01 00:00:00












  • Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150glued.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen TY,Lin TC,Kuo PL,Chen ZR,Cheng HL,Chao YY,Syu JS,Lu FI,Wang CY

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  • Characteristics of concanavalin A-resistant Chinese hamster ovary cells and certain revertants.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cifone MA,Hynes RO,Baker RM

    更新日期:1979-07-01 00:00:00

  • Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Heckman CA,Plummer HK 3rd,Runyeon CS

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ali MA,Chow AK,Kandasamy AD,Fan X,West LJ,Crawford BD,Simmen T,Schulz R

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  • Identification of novel, stage-specific polypeptides associated with the differentiation of mammary epithelial stem cells to alveolar-like cells in culture.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Paterson FC,Rudland PS

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kleinsmith LJ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mulder KM,Brattain MG

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stamato TD,Patterson D

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vidair C,Rubin H

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Le Marer N,Skacel PO

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yang W,Liu R,Xia C,Chen Y,Dong Z,Huang B,Li R,Li M,Xu C

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wharton W,Van Wyk JJ,Pledger WJ

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  • Lens epithelial cell adhesion to lens capsule: a model system for cell-basement membrane interaction.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cammarata PR,Spiro RG

    更新日期:1982-11-01 00:00:00

  • Hypoxia-induced upregulation of eNOS gene expression is redox-sensitive: a comparison between hypoxia and inhibitors of cell metabolism.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hoffmann A,Gloe T,Pohl U

    更新日期:2001-07-01 00:00:00

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    journal_title:Journal of cellular physiology

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    authors: Chen Y,He X,Chen X,Li Y,Ke Y

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    journal_title:Journal of cellular physiology

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    authors: Ardaillou N,Lelongt B,Turner N,Piedagnel R,Baudouin B,Estrade S,Cassingena R,Ronco PM

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  • Smart materials as scaffolds for tissue engineering.

    abstract::In this review, we focused our attention on the more important natural extracellular matrix (ECM) molecules (collagen and fibrin), employed as cellular scaffolds for tissue engineering and on a class of semi-synthetic materials made from the fusion of specific oligopeptide sequences, showing biological activities, wit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Rosso F,Marino G,Giordano A,Barbarisi M,Parmeggiani D,Barbarisi A

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  • Mechanisms controlling neurite outgrowth in a pheochromocytoma cell line: the role of TRPC channels.

    abstract::Transient Receptor Potential Canonical (TRPC) channels are implicated in modulating neurite outgrowth. The expression pattern of TRPCs changes significantly during brain development, suggesting that fine-tuning TRPC expression may be important for orchestrating neuritogenesis. To study how alterations in the TRPC expr...

    journal_title:Journal of cellular physiology

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    authors: Kumar S,Chakraborty S,Barbosa C,Brustovetsky T,Brustovetsky N,Obukhov AG

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    authors: Cruise JL,Cotecchia S,Michalopoulos G

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    pub_type: 杂志文章


    authors: Reem GH,Friend C

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mitsudomi T,Kimura G

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    pub_type: 杂志文章


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