Abstract:
BACKGROUND:Detection of cancer micrometastases is required for improvement of cancer therapy. The aim of this study was to establish a rapid and practical genetic assay to detect micrometastasis in gastric cancer and to assess its clinical significance with respect to prognosis. METHODS:A novel RNA amplification system with transcription-reverse transcription concerted reaction (TRC) was introduced for quantitative detection of cancer-specific carcinoembryonic antigen messenger RNA. The sensitivity and quantitative aspects of the assay were assessed with the full-length carcinoembryonic antigen messenger RNA, a gastric cancer cell line (MKN-45), and metastatic lymph nodes obtained from patients with gastric cancer. Peritoneal lavage fluid specimens that were collected from gastric cancer surgery were subjected to the assay, and the clinical significance of the results was examined for prediction of recurrence and survival. RESULTS:The quantification, sensitivity, and reproducibility of the assay with the TRC reaction were equal to those of quantitative reverse transcriptase-polymerase chain reaction with LightCycler. The most important advantages of the assay were its simplicity and rapidity. Molecular diagnosis of peritoneal lavage fluid by the TRC reaction significantly correlated with depth of invasion, peritoneal metastasis, clinical stage, overall survival, and peritoneal recurrence-free survival. CONCLUSIONS:Molecular diagnosis of peritoneal lavage fluid with the TRC reaction could be a useful prognostic indicator for peritoneal recurrence and survival. Because the TRC reaction is more rapid and simpler than reverse transcriptase-polymerase chain reaction as a format for detecting RNA sequences, it may enhance the genetic diagnosis of cancer micrometastasis and may improve cancer therapy.
journal_name
Ann Surg Oncoljournal_title
Annals of surgical oncologyauthors
Ishii T,Fujiwara Y,Ohnaka S,Hayashi T,Taniguchi H,Takiguchi S,Yasuda T,Yano M,Monden Mdoi
10.1245/ASO.2004.12.043keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
778-85issue
8eissn
1068-9265issn
1534-4681pii
11/8/778journal_volume
11pub_type
杂志文章abstract:BACKGROUND:Approximately 3-5% of patients with thin (≤ 1 mm) cutaneous melanomas develop distant metastases. We sought to identify clinical and pathologic factors associated with distant metastasis and survival in a large number of patients with thin melanoma treated at a single institution. METHODS:We identified pati...
journal_title:Annals of surgical oncology
pub_type: 临床试验,杂志文章
doi:10.1245/s10434-012-2265-y
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Response to neoadjuvant chemotherapy is an independent prognostic factor in locally advanced gastric cancer. However, no prospectively tested pretherapeutic parameters predicting response and/or survival in gastric cancer are available in clinical routine. METHODS:We evaluated the prognostic significance of...
journal_title:Annals of surgical oncology
pub_type: 杂志文章
doi:10.1245/s10434-012-2254-1
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更新日期:2020-09-01 00:00:00
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pub_type: 临床试验,杂志文章
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abstract:: ...
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更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2007-10-01 00:00:00
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journal_title:Annals of surgical oncology
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更新日期:2020-11-01 00:00:00
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1245/s10434-012-2318-2
更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1245/s10434-020-09372-y
更新日期:2020-11-28 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 临床试验,杂志文章
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更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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更新日期:2013-04-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
doi:10.1245/s10434-011-2032-5
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:The intraoperative epidural analgesia (EA) has the potential to reduce stress response to surgical trauma which induces a transient immunoactivation that has a negative impact on the outcome. This study investigates the effect of intraoperative EA versus intravenous analgesia (IA) on the immune function. ME...
journal_title:Annals of surgical oncology
pub_type: 杂志文章,随机对照试验
doi:10.1245/s10434-011-1700-9
更新日期:2011-10-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Patients with borderline resectable pancreatic ductal adenocarcinoma (PDA) represent a high-risk group of patients due to tumor or patient-related characteristics. The optimal management of these patients has not been fully defined. MATERIALS AND METHODS:All patients undergoing evaluation for PDA between 20...
journal_title:Annals of surgical oncology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00