当前位置： SCI文献检索 > EUROPEAN JOURNAL OF PHARMACOLOGY期刊下所有文献 > Lipopolysaccharide activates Akt in vascular smooth muscle cells resulting in induction of inducible nitric oxide synthase through nuclear factor-kappa B activation.

Lipopolysaccharide activates Akt in vascular smooth muscle cells resulting in induction of inducible nitric oxide synthase through nuclear factor-kappa B activation.

Abstract：

:Bacterial lipopolysaccharide and other immunostimulants induce gene expression of an isoform of nitric oxide synthase (iNOS) in vascular smooth muscle cells. This process is dependent on nuclear factor-kappa B (NF-kappa B) activation. The aim of this study was to investigate whether the NF-kappa B and Akt signaling pathways converge to induce iNOS in lipopolysaccharide-stimulated vascular smooth muscle cells. Treatment of vascular smooth muscle cells with lipopolysaccharide plus interferon-gamma (LPS/IFN) caused activation of Akt and NF-kappa B. LPS/IFN caused activation of the iNOS promoter and transcription of iNOS mRNA/protein, resulting in NO production. A pharmacological inhibitor of the phosphoinositide 3-kinase (PI3K)-Akt pathway, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002), inhibited phosphorylation of Akt and suppressed activation of NF-kappa B by attenuating the phosphorylation and degradation of I kappa B. LY294002 thereby inhibited LPS/IFN-induced iNOS expression and NO production. Another inhibitor of the PI3K-Akt pathway, wortmannin, also inhibited NO production in VSMC. LY294002 similarly inhibited interleukin-1 beta- or tumor necrosis factor-alpha-induced NO production. The data indicate that lipopolysaccharide or cytokine stimulation of vascular smooth muscle cells leads to activation of the PI3K-Akt pathway, which then activates the NF-kappa B pathway. Thus, the PI3K-Akt pathway controls the expression of iNOS in lipopolysaccharide- and cytokine-stimulated vascular smooth muscle cells.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Hattori Y,Hattori S,Kasai K

doi

10.1016/j.ejphar.2003.09.034

keywords:

subject

Has Abstract

pub_date

2003-11-28 00:00:00

pages

153-8

issue

2-3

eissn

0014-2999

issn

1879-0712

pii

S0014299903024300

journal_volume

481

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF PHARMACOLOGY文献大全
  • MK-801 powerfully protects against N-methyl aspartate neurotoxicity.

    abstract：:Using the ex vivo chick embryo retina to study the efficacy of antagonists in blocking the excitotoxic effects of excitatory amino acid agonists, we previously identified phencyclidine as the most powerful known anti-excitotoxin. Here we show that MK-801 is 5 times more powerful than phencyclidine as an anti-excitotox...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(87)90552-8

    authors： Olney J,Price M,Salles KS,Labruyere J,Frierdich G

    更新日期：1987-09-23 00:00:00

  • Relaxing effects induced by the soluble guanylyl cyclase stimulator BAY 41-2272 in human and rabbit corpus cavernosum.

    abstract：:5-Cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine (BAY 41-2272) is a potent soluble guanylyl cyclase stimulator in a nitric oxide (NO)-independent manner. The relaxant effect of BAY 41-2272 was investigated in rabbit and human corpus cavernosum in vitro. BAY 41-2272 (0.01-10 micr...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2003.08.012

    authors： Baracat JS,Teixeira CE,Okuyama CE,Priviero FB,Faro R,Antunes E,De Nucci G

    更新日期：2003-09-12 00:00:00

  • Emetic, central nervous system, and pulmonary activities of rolipram in the dog.

    abstract：:Rolipram was characterized for its emetic, behavioral, cardiovascular and pulmonary activities in dogs, to assess its systemic pharmacology and potential bronchodilatory selectivity. At doses > or = 0.1 mg/kg i.v., rolipram induced emesis, anxiety, and stepping behaviors in conscious dogs, and increased heart rate and...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00457-2

    authors： Heaslip RJ,Evans DY

    更新日期：1995-11-24 00:00:00

  • Bronchoconstriction and endogenous nitric oxide in isolated lungs of spontaneously hypertensive rats.

    abstract：:Bronchoconstrictor responses were measured in lungs isolated from spontaneously hypertensive (SHR) and normotensive rats, perfused via the airways. Lungs from SHRs were more responsive than lungs from normotensive rats to methacholine, 5-hydroxytryptamine (5-HT), arachidonic acid or prostaglandin H(2). The responses o...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2004.02.005

    authors： Kwasniewski FH,Landgraf RG,Bakhle YS,Jancar S

    更新日期：2004-03-19 00:00:00

  • Current and future treatment strategies for iron overload cardiomyopathy.

    abstract：:Iron overload cardiomyopathy is the major cause of death in transfusion-dependent thalassemia (TDT) patients. Growing evidence demonstrates that combined iron chelators, or the combination of an iron chelator with antioxidant(s) are effective in diminishing myocardial iron deposition and attenuating cardiac dysfunctio...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejphar.2015.08.017

    authors： Wongjaikam S,Kumfu S,Chattipakorn SC,Fucharoen S,Chattipakorn N

    更新日期：2015-10-15 00:00:00

  • Tonic GABA(A) receptor-mediated neurotransmission in the dorsal vagal complex regulates intestinal motility in rats.

    abstract：:Vagal motor outflow from the dorsal vagal complex is important in the regulation of intestinal motility. The aim of our study was to test the hypothesis that within the dorsal vagal complex, tonic GABA(A)-receptor mediated neurotransmission modulates intestinal motility. The GABA(A) receptor antagonist, bicuculline (m...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00071-5

    authors： Greenwood-Van Meerveld B,Barron KW

    更新日期：1998-04-10 00:00:00

  • The tachykinin NK1 receptor antagonist PD 154075 blocks cisplatin-induced delayed emesis in the ferret.

    abstract：:The activity of a selective tachykinin NK1 receptor antagonist, PD 154075 ([(2-benzofuran)-CH2OCO]-(R)-alpha-MeTrp-(S)-NHCH(CH3) Ph), was examined in radioligand binding studies, in a [Sar9,Met(O2)11]substance P-induced foot-tapping model in the gerbil, and in cisplatin-induced acute and delayed emesis in the ferret. ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(96)00950-8

    authors： Singh L,Field MJ,Hughes J,Kuo BS,Suman-Chauhan N,Tuladhar BR,Wright DS,Naylor RJ

    更新日期：1997-02-26 00:00:00

  • NMDA and AMPA/kainate receptors are involved in the anticonvulsant activity of riluzole in DBA/2 mice.

    abstract：:The anticonvulsant activity of riluzole against sound-induced seizures was studied in the DBA/2 mouse model. Riluzole (0.1-4 mg kg(-1), intraperitoneal (i.p.)) produced dose-dependent effects with ED(50) values for the suppression of tonic, clonic and wild running phases of 0.72, 1.38 and 2.71 mg kg(-1), respectively....

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(00)00709-3

    authors： De Sarro G,Siniscalchi A,Ferreri G,Gallelli L,De Sarro A

    更新日期：2000-11-10 00:00:00

  • Reduced levels of 5-HT3 receptor recognition sites in the putamen of patients with Huntington's disease.

    abstract：:The present study assessed 5-HT3 receptor recognition site levels in homogenates of putamen derived from patients with clinically and neurochemically diagnosed Huntington's disease or Parkinson's disease and those from age-, sex- and post-mortem delay-matched neurologically and psychiatrically normal patients to inves...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(93)90073-q

    authors： Steward LJ,Bufton KE,Hopkins PC,Davies WE,Barnes NM

    更新日期：1993-09-28 00:00:00

  • Neuroprotective effect of WIN 55,212-2 pretreatment against focal cerebral ischemia through activation of extracellular signal-regulated kinases in rats.

    abstract：:It is well documented that cannabinoid receptor agonist WIN 55,212-2 had protective effect against cerebral ischemic injury. Our previous study indicated that WIN 55,212-2 pretreatment induced ischemic tolerance to focal cerebral ischemia in a dose-dependent manner. The aim of the present study was to investigate the ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2010.07.024

    authors： Hu B,Wang Q,Chen Y,Du J,Zhu X,Lu Y,Xiong L,Chen S

    更新日期：2010-10-25 00:00:00

  • Effects of oxidized low-density lipoprotein on differentiation of mouse neural progenitor cells into neural cells.

    abstract：:In Alzheimer's disease (AD), a decline in function of neural progenitor cells (NPCs) results in a reduced capacity for neural regeneration. It has been shown that plasma oxidized low-density lipoprotein (ox-LDL) levels are positively correlated with severity in patients with AD. However, the direct effects of ox-LDL o...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2020.173456

    authors： Ishizuka T,Nagata W,Nomura-Takahashi S,Satoh Y

    更新日期：2020-12-05 00:00:00

  • Prostacyclin prevents ischemia-induced increase of lactate and cyclic AMP in ischemic myocardium.

    abstract：:The effect of prostacyclin (PGI2, 0.5 nmol . kg-1 . min-1,i.v.) on myocardial metabolism was studied in cats subjected to 5 h of myocardial ischemia (MI) and compared to vehicle-treated MI cats. MI was followed by a 52% decrease in ATP and a concomitant increase (2-3 fold) in lactate and lactate/pyruvate ratio in the ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(81)90454-4

    authors： Rösen R,Rösen P,Ohlendorf R,Schrör K

    更新日期：1981-02-19 00:00:00

  • Long-term blockade of the expression of cocaine sensitization by ondansetron, a 5-HT(3) receptor antagonist.

    abstract：:Intermittent cocaine administration induces sensitization (reverse tolerance) to its behavioral effects. The mechanism(s) mediating sensitization is not clear, however, previous research has implicated 5-HT(3) receptors in the expression of sensitization. The present experiment evaluated the ability of the 5-HT(3) rec...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(99)00926-7

    authors： King GR,Xiong Z,Douglass S,Ellinwood EH

    更新日期：2000-04-07 00:00:00

  • Effects of S 21403 on hormone secretion from isolated rat pancreas at different glucose concentrations.

    abstract：:We investigated the in vitro effects of therapeutical concentrations of S 21403 (a succinic acid derivative also known as KAD 1229 and mitiglinide) on insulin and glucagon secretion during a metabolic stimulus (glucose rising from 5 to 8.33 mM) or at a stable 2.22 mM glucose using the isolated perfused rat pancreas mo...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(02)02620-1

    authors： Gregorio F,Ambrosi F,Boemi M,Carle F,Filipponi P

    更新日期：2002-12-05 00:00:00

  • Tissue-specific effect of clofibrate on rat lipogenic enzyme gene expression.

    abstract：:Fibrate derivatives are commonly used to treat hyperlipidaemia; however, the mechanism of the antilipidaemic action of these drugs is still unknown. The effect of clofibrate (fibrate derivative) administration for 14 days on lipogenesis and on malic enzyme (EC 1.1.1.40) and fatty acid synthase (EC 2.3.1.85) gene expre...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(99)00129-6

    authors： Karbowska J,Kochan Z,Zelewski L,Swierczynski J

    更新日期：1999-04-16 00:00:00

  • The role of ATP-sensitive potassium channel on acute urinary retention and subsequent catheterization in the rat.

    abstract：:We investigated the role of K(ATP) channel on acute urinary retention (AUR) induced bladder dysfunction. Eight-week-old female Sprague-Dawley rats were divided into seven groups: a sham-operated control group, an AUR group, and five AUR groups treated with: two different K(ATP) channel openers namely nicorandil (3 or ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2010.02.052

    authors： Ohmasa F,Saito M,Shimizu S,Taniguchi S,Dimitriadis F,Satoh I,Kinoshita Y,Satoh K

    更新日期：2010-06-10 00:00:00

  • The role of endogenous opioids in the luteinizing hormone surge in rats: studies with clocinnamox, a long-lasting opioid receptor antagonist.

    abstract：:Endogenous opioid peptides have been demonstrated to regulate luteinizing hormone (LH) secretion in a variety of species. Studies in rodents suggest a role of opioid peptide systems in controlling the timing of the LH surge, which is entrained to the circadian rhythm. The current studies utilize clocinnamox, a novel l...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00341-0

    authors： Lieberman PB,Woods JH,Young EA

    更新日期：1998-07-03 00:00:00

  • The specific effect of Mn2+ on the tonic components of receptor-mediated contractions in isolated vas deferens of the guinea pig.

    abstract：:Intracellular accumulation of Mn2+ augmented the contractions induced by norepinephrine and acetylcholine in the guinea pig isolated vas deferens. Contractions repeatedly induced by norepinephrine, acetylcholine, or a high concentration of K+ decreased depending on the incubation time in Ca(2+)-free medium. The rate o...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00664-8

    authors： Tsunobuchi-Ushijima H,Gomi Y

    更新日期：1996-01-11 00:00:00

  • Stability of the vasopressin V2 receptor-adenylyl cyclase system in rat kidney.

    abstract：:In the Brattleboro rat with diabetes insipidus vasopressin V2 receptor mRNA and the mRNA of various adenylyl cyclase (AC) isoforms are moderately reduced compared with those of normal rats. In the present study renal vasopressin V2 receptor mRNA was modestly higher (by 34%), as was expression of AC 5, 6 and 9 mRNAs (u...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(97)01461-1

    authors： Shen T,Laycock J,Suzuki Y,Defer N,Hanoune J

    更新日期：1998-01-02 00:00:00

  • The synergetic effect of edaravone and borneol in the rat model of ischemic stroke.

    abstract：:Free radical production contributes to the early ischemic response and the neuroinflammatory response to injury initiates the second wave of cell death following ischemic stroke. Edaravone is a free radical scavenger, and borneol has shown anti-inflammatory effect. We investigated the synergistic effect of these two d...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2014.06.035

    authors： Wu HY,Tang Y,Gao LY,Sun WX,Hua Y,Yang SB,Zhang ZP,Liao GY,Zhou QG,Luo CX,Zhu DY

    更新日期：2014-10-05 00:00:00

  • Effects of intracerebral quinpirole on locomotion in rats.

    abstract：:The effects of the dopamine D2 receptor agonist quinpirole (LY 171555) on locomotor activity and margin time (thigmotaxis or wall-hugging) were measured for 2 h in rats injected either s.c. (vehicle, 0.02, 2.0 mg/kg) or directly into either the dorsal striatum or nucleus accumbens (vehicle, 0.1, 1.0, 10, 20 or 40 micr...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(92)90091-h

    authors： Van Hartesveldt C,Cottrell GA,Potter T,Meyer ME

    更新日期：1992-04-07 00:00:00

  • Drinking sucrose or saccharin enhances sensitivity of rats to quinpirole-induced yawning.

    abstract：:Diet can impact sensitivity of rats to some of the behavioral effects of drugs acting on dopamine systems. The current study tested whether continuous access to sucrose is necessary to increase yawning induced by the dopamine receptor agonist quinpirole, or if intermittent access is sufficient. These studies also test...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.07.036

    authors： Serafine KM,Bentley TA,Kilborn DJ,Koek W,France CP

    更新日期：2015-10-05 00:00:00

  • Comparison of the behavioral effects of bupropion and psychostimulants.

    abstract：:Psychostimulant abuse has been a serious social problem worldwide for a long time. Bupropion, which is used as an antidepressant and to aid smoking cessation in the US, is considered to have psychostimulant-like activity. Although activation of the dopaminergic system induces several behavioral effects and bupropion c...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.07.046

    authors： Mori T,Shibasaki M,Ogawa Y,Hokazono M,Wang TC,Rahmadi M,Suzuki T

    更新日期：2013-10-15 00:00:00

  • Analogues of F8Famide resistant to degradation, with high affinity and in vivo effects.

    abstract：:Four analogues of Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2, a mammalian FMRFamide-like peptide with antiopiate properties, were synthesized with N-terminus modifications and were shown to have high affinity for F8Famide binding sites. The degradation rate of these analogues in mouse brain slices was 3 times lower than that...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(92)90463-e

    authors： Gicquel S,Mazarguil H,Allard M,Simonnet G,Zajac JM

    更新日期：1992-11-03 00:00:00

  • Activation of the 5-HT1A receptor subtype increases rat plasma ACTH concentration.

    abstract：:Serotonergic (5-HT) neuronal pathways regulate the release of adrenocorticotropin hormone (ACTH) from the pituitary gland probably through the action of hypothalamic corticotropin-releasing hormone (CRH). 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), a selective 5-HT1A receptor agonist, dose dependently (0.016-3...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(88)90178-1

    authors： Gilbert F,Brazell C,Tricklebank MD,Stahl SM

    更新日期：1988-03-15 00:00:00

  • Biochemical and behaviour changes induced by acute stress in a chronic variate stress model of depression: the effect of amitriptyline.

    abstract：:This paper examines the biochemical and behaviour changes induced by an acute stress (five 10-s, 1-mA foot-shocks) in three groups of rats: (1) never stressed, (2) subjected to chronic variate stress for 20 days, (3) subjected to the same chronic stress and treated with 5 mg/kg per day amitriptyline. After 15 min, acu...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00172-h

    authors： Ferretti C,Blengio M,Gamalero SR,Ghi P

    更新日期：1995-06-23 00:00:00

  • MDL 74,180 reduces cerebral infarction and free radical concentrations in rats subjected to ischaemia and reperfusion.

    abstract：:The protective effect of MDL 74,180 (2,3-dihydro-2,2,4,6, 7-pentamethyl-3-(4-methylpiperazino)-methyl-1-benzofuran-5-ol dihydrochloride) and alpha-tocopherol analogue free radical scavenger, against cerebral ischaemia and reperfusion in conscious rats has been demonstrated. Tissue damage following middle cerebral arte...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00807-1

    authors： Cowley DJ,Lukovic L,Petty MA

    更新日期：1996-03-18 00:00:00

  • Alpha 1-adrenoceptor subtypes mediating noradrenaline-induced contraction of pulmonary artery from pulmonary hypertensive rats.

    abstract：:The effect of monocrotaline-induced pulmonary hypertension on alpha(1)-adrenoceptor-mediated contractions of pulmonary artery segments was studied. In control and monocrotaline-treated rats, noradrenaline evoked concentration-dependent contractions of the pulmonary artery. There was no change in the potency and affini...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2003.10.001

    authors： Oriowo MA,Chandrasekhar B,Kadavil EA

    更新日期：2003-12-15 00:00:00

  • Dizocilpine-induced accuracy deficits in a visual signal detection task are not present following D-cycloserine administration in rats.

    abstract：:The N-methyl-D-aspartate (NMDA) receptor system is thought to be underactive in schizophrenia which may contribute to attentional dysfunction in this disease. In a visual signal detection task that required discrimination of signaled-trials from trials with no signal, the NMDA receptor antagonist, dizocilpine (0.05 mg...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2007.08.037

    authors： Howe WM,Burk JA

    更新日期：2007-12-22 00:00:00

  • Effects of chlorogenic acid and its metabolites on the sleep-wakefulness cycle in rats.

    abstract：:The effect of chlorogenic acid on the sleep-wakefulness cycle in rats was investigated in comparison with those of caffeic acid (the metabolite of chlorogenic acid) and dihydrocaffeic acid (the metabolite of caffeic acid). A significant prolongation of sleep latency was observed with chlorogenic acid and caffeic acid ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2004.09.054

    authors： Shinomiya K,Omichi J,Ohnishi R,Ito H,Yoshida T,Kamei C

    更新日期：2004-11-19 00:00:00