Antimicrobial activity of human follicular fluids.

Abstract:

:The aim of this study was to explore the antimicrobial activity of human follicular fluid (HFF), to test the hypothesis that different strains of the same bacterial species could display different patterns of susceptibility to antimicrobial action of HFF, and to preliminarily investigate the possible mechanism of antimicrobial action of this fluid. Antimicrobial activity of 60 samples of HFF toward 30 Streptococcus agalactiae strains was determined by the agar diffusion method and broth dilution method. To explore the mechanism of antimicrobial activity, biochemical analyses were performed with selected fluid samples. The obtained results indicate that 38.3% fluid samples did not inhibit bacterial growth, 53.3% showed moderate and 8.3% high antimicrobial activity. The tested effect of HFF on S. agalactiae strains was bactericidal and was not strain dependent. Lysozyme activity was detected in HFF exhibiting antimicrobial activity. There were no statistically significant differences in concentrations of estradiol, progesterone, transferrin, iron, total protein and albumin levels among tested samples regardless of the different rate of antimicrobial activity. The obtained results indicate that lysozyme is most probably a crucial antibacterial agent in this fluid; however, some other still unidentified factors may contribute to it.

journal_name

Gynecol Obstet Invest

authors

Stepanović S,Djukić S,Veljković M,Arsić B,Garalejić E,Ranin L

doi

10.1159/000074103

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

173-8

issue

3

eissn

0378-7346

issn

1423-002X

pii

74103

journal_volume

56

pub_type

杂志文章
  • Vulvar papillomatosis, aceto-white lesions, and normal-looking vulvar mucosa evaluated by microscopy and human papillomavirus analysis.

    abstract:BACKGROUND AND OBJECTIVES:To assess the presence of human papillomavirus (HPV) DNA in vulvar papillomatosis, since some women with this complaint have symptoms associated with HPV infection, such as itching, burning, and dyspareunia. GOAL:To reassure the patients that they do not have a transmissible viral disease, by...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000292350

    authors: Strand A,Wilander E,Zehbe I,Kraaz W,Rylander E

    更新日期:1995-01-01 00:00:00

  • Subcutaneous implantation of pure crystalline estradiol pellets for conception control.

    abstract:OBJECTIVES:Assessment of the contraceptive effectiveness of pure estradiol pellets implanted annually under the skin, thus avoiding the 'first passage phenomenon' through the liver, using a modification of Emperaire and Greenblatt's method. STUDY DESIGN:228 women wishing birth control for 1 year or more were included....

    journal_title:Gynecologic and obstetric investigation

    pub_type: 临床试验,杂志文章

    doi:10.1159/000082652

    authors: Oettinger M,Barak S,Oettinger-Barak O,Ophira E

    更新日期:2005-01-01 00:00:00

  • Urinary excretion, osmolarity and electrolytes after bolus-injection of fenoterol in female rabbits.

    abstract::The effects of bolus injections of 1.0-80.0 micrograms/kg body weight fenoterol on urinary excretion, osmolarity and electrolytes were studied in unanesthetized, water-loaded rabbits. In animals infused initially with isotonic solution over 2 h with 60 ml/h and thereafter over 10 h with 45 ml/h, urine excretion was 53...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000299169

    authors: Grospietsch G,Ulbrich R,Saul U,Fenske M,Ensink FB,Kuhn W

    更新日期:1984-01-01 00:00:00

  • Safety of therapeutic doses of tinzaparin during pregnancy.

    abstract:BACKGROUND/AIMS:Low molecular weight heparin is recommended for the treatment of venous thromboembolism (VTE) during pregnancy. However, there are few reliable data regarding the safety of therapeutic doses of tinzaparin in this setting. The objective of this study was to assess the safety of once-daily therapeutic dos...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000367846

    authors: Parent F,Deruelle P,Sanchez O,Meyer G,Girard P,Jilwan F,Boyer-Neumann C,Wolf M,Simonneau G

    更新日期:2015-01-01 00:00:00

  • Timing of group B streptococcus screening in pregnancy: a systematic review.

    abstract:BACKGROUND:Group B streptococcus (GBS) is an important cause of neonatal sepsis. Guidelines advise to collect cultures at 35-37 weeks' gestation and to administer intrapartum antibiotic prophylaxis in case of GBS-positive cultures, as well as in all preterm deliveries. Improved effectiveness of antenatal cultures might...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,评审

    doi:10.1159/000265942

    authors: Valkenburg-van den Berg AW,Houtman-Roelofsen RL,Oostvogel PM,Dekker FW,Dörr PJ,Sprij AJ

    更新日期:2010-01-01 00:00:00

  • Body mass index and severe postpartum hemorrhage.

    abstract:BACKGROUND/AIMS:The purpose of this study was to determine the relationship between maternal characteristics and severe postpartum hemorrhage (PPH). METHODS:Medical records of women who delivered at Duke University Hospital between 2001 and 2004 with an ICD-9 code for PPH were reviewed. Women with PPH who received blo...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000329335

    authors: Paglia MJ,Grotegut CA,Johnson LN,Thames B,James AH

    更新日期:2012-01-01 00:00:00

  • Modified hysteroscopic myomectomy of large submucous fibroids.

    abstract:OBJECTIVE:To compare the efficacy, feasibility, operative time and possible complications of a modified technique of hysteroscopic removal of large submucus myomata versus traditional morcellation technique, and to assess the efficacy of preoperative sonohysterography (SHG). DESIGN:A prospective comparative study. SE...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000074451

    authors: Darwish A

    更新日期:2003-01-01 00:00:00

  • Doppler flow velocity waveforms and vasoactive effects of serotonin in human umbilical arteries.

    abstract::The vasoactive effect of serotonin was investigated by in vitro perfusion in human umbilical arteries in which antenatal Doppler ultrasound examination revealed abnormal velocity waveforms. The results were compared to those obtained in preparations from normotensive uncomplicated term pregnancies as well as from pret...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000052885

    authors: Haugen G,Rognerud H

    更新日期:2001-01-01 00:00:00

  • Human chorionic gonadotropin in cervicovaginal secretions as a predictor of preterm birth.

    abstract:OBJECTIVE:To compare the qualitative (QL) and quantitative (QN) levels of betahCG in cervicovaginal secretions as predictors of preterm birth. METHODS:The study included 100 women. QL and QN assays were done with velocit kit and ELISA, respectively. RESULTS:A positive QL assay had sensitivity of 78%, specificity 95%,...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000189917

    authors: Rengaraj G,Guleria K,Suneja A,Gambhir JK

    更新日期:2009-01-01 00:00:00

  • Can a low-lying placenta be the risk factor of cervical incompetence? A preliminary study on the relationship between placenta insertion and anatomical status of uterine cervix.

    abstract::An attempt was made to verify the hypothesis that a low-lying placenta may be a factor leading to the dynamic form of cervical incompetence. A vaginal evaluation of the cervix was made in 143 pregnant women who by means of ultrasonographic method were found to have low-lying placentas. In as many as 68 out of the 143 ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000298964

    authors: Uszyński M,Majczyński T,Uszyński W

    更新日期:1985-01-01 00:00:00

  • Experience with isotonic 2.2% glycine as distension medium for hysteroscopic endomyometrial resection.

    abstract::The feasibility of performing hysteroscopic endomyometrial resection using isotonic 2.2% glycine as distension medium was studied in 181 consecutive operations. A standard continuous flow loop-resectoscope was used. Distension was achieved by means of a gravity-fed system and glycine flowed out under its own pressure....

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000010119

    authors: Tapper AM,Heinonen PK

    更新日期:1999-01-01 00:00:00

  • Transcutaneous and arterial carbon dioxide tension at various conditions of fetal stress in lambs.

    abstract::In an acute fetal lamb model the relation between arterial PCO2 (PaCO2), transcutaneous PCO2 (tcPCO2) and pH was studied at different conditions of stress. Occlusion of the maternal common iliac artery for 8 min, umbilical cord obstruction for 5 min and placental embolization were performed subsequently, every time wi...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000291808

    authors: Bergmans MG,Stevens GH,Keunen H,Hasaart TH

    更新日期:1997-01-01 00:00:00

  • Relationship between metabolic syndrome and uterine leiomyomas: a case-control study.

    abstract:BACKGROUND/AIMS:Uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being. The pathophysiology and epidemiology of fibroids are poorly understood. Obesity and elevated blood pressure have been reported to be predisposing factors. In this study, we investigat...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000114250

    authors: Takeda T,Sakata M,Isobe A,Miyake A,Nishimoto F,Ota Y,Kamiura S,Kimura T

    更新日期:2008-01-01 00:00:00

  • Advantage or disadvantage of episiotomy compared with spontaneous perineal laceration.

    abstract::In a prospective clinical investigation of 2,144 deliveries, we elucidate the indications for episiotomy and how different methods of anesthesia affect the frequency of episiotomy and the perineal problems after episiotomy compared with those after spontaneous perineal laceration. We found a significantly higher infec...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293161

    authors: Larsson PG,Platz-Christensen JJ,Bergman B,Wallstersson G

    更新日期:1991-01-01 00:00:00

  • Management of supraventricular tachycardia during hexoprenaline therapy for preterm labour: benefit of cardioselective beta blockade?

    abstract::A 29-year-old woman presented with preterm labour at 32 weeks of gestation. Tocolytic treatment was started with intravenous hexoprenaline. Twenty-four hours after initiation of treatment, the patient developed supraventricular tachycardia, resistant to digoxin and verapamil. Medical treatment with metoprolol finally ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000292411

    authors: Frigo P,Eppel W,Frank A,Ulm M,Golaszewski T,Gruber W

    更新日期:1995-01-01 00:00:00

  • Blood loss during transvaginal oocyte retrieval.

    abstract:BACKGROUND/AIMS:Limited information is available regarding the quantity of blood loss associated with uncomplicated transvaginal oocyte retrieval. The aim of the present study was evaluating the quantity of such a loss. METHODS:One hundred and fifty consecutive women undergoing oocyte retrieval were recruited. They un...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000158649

    authors: Ragni G,Scarduelli C,Calanna G,Santi G,Benaglia L,Somigliana E

    更新日期:2009-01-01 00:00:00

  • Diagnostic aid of endometrium biopsy.

    abstract::The 784 cases, from which 701 complained of abnormal uterine bleeding, were reviewed by age groups, presenting symptoms, sex steroid medication and endometrial findings. Endometrial polyps and endometritis, which showed an incidence of 17.5 and 10.0%, respectively, were correlated with clinicopathologic findings. Peri...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000299980

    authors: van Bogaert LJ

    更新日期:1979-01-01 00:00:00

  • Uterine myoma causing uremia in a 15-year-old girl.

    abstract::Uterine myoma leading to uremia in young women below 16 years of age is an extremely rare condition. On reviewing the literature we have not been able to find any case similar to the one described below. ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293043

    authors: Heimer G,Axelsson O,Johnson P

    更新日期:1991-01-01 00:00:00

  • Is There a Benefit to Culturing Intra-Uterine Devices in Pelvic Inflammatory Disease?

    abstract:AIMS:The study aimed to compare the clinical course and disease severity between culture positive and culture negative patients with intra-uterine devices (IUD)-associated pelvic inflammatory disease (PID). METHODS:A retrospective study of all IUD-associated PID patients admitted to tertiary medical center between 201...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000490666

    authors: Fouks Y,Many A,Amit S,Levin I,Meiri A,Shinar S

    更新日期:2019-01-01 00:00:00

  • Metastatic choriocarcinoma presenting in a gravid woman.

    abstract::Choriocarcinoma coexistent with an intrauterine pregnancy is rare and requires decisions affecting mother and child to treat this aggressive tumor. An instance of this situation is presented to highlight clinical circumstances that warrant departure from accepted dogma. Pathologic aspects of choriocarcinoma are also d...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000363234

    authors: Byrd K,Seay R,Bechert CJ,Maxwell GL,Bicher A

    更新日期:2015-01-01 00:00:00

  • Elevated Expression of T-Cell Immunoglobulin and Mucin Domain 3 on T Cells from Peripheral Blood in Patients with Cervical Carcinoma.

    abstract:OBJECTIVE:To investigate the expression of T-cell immunoglobulin and mucin domain 3 (TIM-3) on peripheral T cells of cervical carcinoma patients. METHODS:Peripheral blood samples from 15 high-grade cervical squamous intraepithelial lesion (HSIL) patients, 24 cervical carcinoma patients, and 21 healthy controls were co...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000511440

    authors: Li J,Sun XF,Shen Y,Yang Q,Dai SY

    更新日期:2020-12-04 00:00:00

  • Fetal heart rate decelerations precede a decrease in fetal oxygen content.

    abstract::To determine the relationship between abnormal fetal heart rate (FHR) patterns, fetal oxygen content by reflectance pulse oximetry, and the effects of maternal oxygen administration, 158 intrapartum women consisting of 120 women with normal FHR patterns, and 38 women with varying degrees of variable FHR decelerations ...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000291404

    authors: Izumi A,Minakami H,Sato I

    更新日期:1997-01-01 00:00:00

  • Clinical and prognostic significance of human papillomavirus in a Chinese population of cervical cancers.

    abstract:OBJECTIVE:To investigate the clinical and prognostic significance of human papillomavirus (HPV) in a Chinese population of cervical cancers. METHODS:We studied 121 cervical cancer tissue samples from patients treated at our hospital. Identification and typing of HPV were done by polymerase chain reaction (PCR) using c...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000052925

    authors: Lo KW,Cheung TH,Chung TK,Wang VW,Poon JS,Li JC,Lam P,Wong YF

    更新日期:2001-01-01 00:00:00

  • Guillain-barré syndrome in pregnancy: early diagnosis and treatment is essential for a favorable outcome.

    abstract::Guillain-Barré syndrome (GBS) is an acute or subacute demyelinating polyneuropathy that rarely complicates pregnancy, but increases maternal and perinatal morbidity. A 15-year-old pregnant woman was admitted with ascending paralysis and acellular lumbar puncture suggestive of GBS. She required mechanical ventilation f...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000209215

    authors: Campos da Silva F,de Moraes Paula G,Dos Santos Esteves Automari CV,Mendes de Almeida DS,Ubirajara Cavalcanti Guimarães R

    更新日期:2009-01-01 00:00:00

  • A Randomized Controlled Trial on Efficacy and Safety of Trocar-Site Infiltration with Lidocaine for Postoperative Pain Relief after Diagnostic Laparoscopy.

    abstract:BACKGROUND/AIMS:To compare the efficacy and safety of trocar-site infiltration with lidocaine and no intervention for postoperative pain relief after diagnostic laparoscopy. METHODS:A randomized clinical trial of women with infertility scheduled for diagnostic laparoscopy without additional procedures in Life Institut...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000490565

    authors: Joe-Ikechebelu NN,Eleje GU,Ugwu EO,Okafor CD,Nwachukwu CE,Okam PC,Ogboji OE,Ikechebelu JI

    更新日期:2019-01-01 00:00:00

  • Tension-free vaginal tape procedure: an effective minimally invasive operation for the treatment of recurrent stress urinary incontinence?

    abstract::The aim of this study was to evaluate the results of tension-free vaginal tape (TVT) surgery in women with recurrent stress urinary incontinence (SUI). Fifty-one women with recurrent SUI were treated with TVT and followed prospectively for a minimum of 2 years according to a protocol. Twenty percent of the women had a...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000072942

    authors: Kuuva N,Nilsson CG

    更新日期:2003-01-01 00:00:00

  • The influence of tocolytic therapy on serum digoxin-like immunoreactive substance concentration.

    abstract::Digoxin-like immunoreactive substance (DLIS) has been proposed to have a role in what seems to be the timely onset of labor. The relationship between the DLIS in patients with preterm labor and the success of tocolytic therapy with ritodrine and nifedipine has been studied. Using a commercially available radioimmunoas...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000292511

    authors: Yapar EG,Ayhan A

    更新日期:1994-01-01 00:00:00

  • Effects of caffeine ingestion during pregnancy.

    abstract::We studied the effects of caffeine ingestion during pregnancy. A total number of 9,921 healthy pregnant women with a gestational age after 24 weeks were subjected to the study. The women who drank more than 5 cups of coffee per day had a high incidence of impending abortion, premature labor, and fetuses small for gest...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000299032

    authors: Furuhashi N,Sato S,Suzuki M,Hiruta M,Tanaka M,Takahashi T

    更新日期:1985-01-01 00:00:00

  • Use of highly evacuated Redon drains after gynecologic laparotomies.

    abstract::Highly evacuated (980 mbar) wound drainage systems according to Redon were used in 100 gynecologic laparotomies. In 96% of cases, no flask exchange was required before the system was removed. Bacteriological specimens from various locations of the Redon system showed minimal colonization with pathogenic bacteria. No c...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000293758

    authors: Grillo M,Riedel HH,Lehmann-Willenbrock E,Höller C

    更新日期:1988-01-01 00:00:00

  • Postmenopausal hormone replacement therapy use decreases oxidative protein damage.

    abstract:OBJECTIVES:The aim of this study is to evaluate oxidative protein damage (OPD) by investigating protein carbonyl (PCO) and nitrotyrosine (NT) levels, oxidative stress by total thiol (T-SH), erythrocyte glutathione (GSH) and nitric oxide (NO) levels in women receiving hormone replacement therapy (HRT). MATERIALS AND ME...

    journal_title:Gynecologic and obstetric investigation

    pub_type: 杂志文章

    doi:10.1159/000067718

    authors: Telci A,Cakatay U,Akhan SE,Bilgin ME,Turfanda A,Sivas A

    更新日期:2002-01-01 00:00:00