Abstract:
:Growing Streptococcus gordonii Spp+ phase variants, which have normal levels of glucosyltransferase (GTF) activity, use sucrose to promote their accumulation on surfaces by forming a cohesive bacterium-insoluble glucan polymer mass (BPM). Spp- phase variants, which have lower levels of GTF activity, do not form BPMs and do not remain in BPMs formed by Spp+ cells when grown in mixed cultures. To test the hypothesis that segregation of attached Spp+ and unattached Spp- cells was due to differences in adhesiveness, adhesion between washed, [3H]thymidine-labeled cells and preformed BPM substrata was measured. Unexpectedly, the results showed that cells of both phenotypes, as well as GTF-negative cells, attached equally well to preformed BPMs, indicating that attachment to BPMs was independent of cell surface GTF activity. Initial characterization of this binding interaction suggested that a protease-sensitive component on the washed cells may be binding to lipoteichoic acids sequestered in the BPM, since exogenous lipoteichoic acid inhibited adhesion. Surprisingly, the adhesion of both Spp+ and Spp- cells was markedly inhibited in the presence of sucrose, which also released lipoteichoic acid from the BPM. These in vitro findings suggest that, in vivo, sucrose and lipoteichoic acid may modify dental plaque development by enhancing or inhibiting the attachment of additional bacteria.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Vickerman MM,Jones GWdoi
10.1128/IAI.60.10.4301-4308.1992keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
4301-8issue
10eissn
0019-9567issn
1098-5522journal_volume
60pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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更新日期:1977-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1384-1390.1990
更新日期:1990-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.3.1088-1094.1997
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.635-644.1979
更新日期:1979-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.672-675.1978
更新日期:1978-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2006-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.745-752.1985
更新日期:1985-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2237-2240.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.286-289.1984
更新日期:1984-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1978-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.231-243.1980
更新日期:1980-10-01 00:00:00