Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells.

abstract：:The mechanism of Ca2+-dependent protein-protein interaction and enzyme activation by calmodulin was investigated with the phosphoprotein phosphatase, calcineurin. Dimethylaminonaphthalene (dansyl)-calmodulin, a fluorescent derivative used to monitor complex formation, produced similar maximal activation (10- to 12-fol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kincaid RL,Vaughan M

更新日期：1986-03-01 00:00:00

abstract：:In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doelling JH,Gaudino RJ,Pikaard CS

更新日期：1993-08-15 00:00:00

abstract：:In the manufacture of chemical feedstocks and subsequent processing into derivatives and materials, the U.S. chemical industry sets the current standard of excellence for technological competitiveness. This world-class leadership is attributed to the innovation and advancement of chemical engineering process technolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell JW

更新日期：1992-02-01 00:00:00

abstract：:The three lipin phosphatidate phosphatase (PAP) enzymes catalyze a step in glycerolipid biosynthesis, the conversion of phosphatidate to diacylglycerol. Lipin-1 is critical for lipid synthesis and homeostasis in adipose tissue, liver, muscle, and peripheral nerves. Little is known about the physiological role of lipin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dwyer JR,Donkor J,Zhang P,Csaki LS,Vergnes L,Lee JM,Dewald J,Brindley DN,Atti E,Tetradis S,Yoshinaga Y,De Jong PJ,Fong LG,Young SG,Reue K

更新日期：2012-09-11 00:00:00

abstract：:We investigated the ultrafast structural transitions of the heme induced by nitric oxide (NO) binding for several heme proteins by subpicosecond time-resolved resonance Raman and femtosecond transient absorption spectroscopy. We probed the heme iron motion by the evolution of the iron-histidine Raman band intensity af...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruglik SG,Yoo BK,Franzen S,Vos MH,Martin JL,Negrerie M

更新日期：2010-08-03 00:00:00

abstract：:We have determined the packing efficiency at the protein-water interface by calculating the volumes of atoms on the protein surface and nearby water molecules in 22 crystal structures. We find that an atom on the protein surface occupies, on average, a volume approximately 7% larger than an atom of equivalent chemical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerstein M,Chothia C

更新日期：1996-09-17 00:00:00

abstract：:The POT1-TPP1 heterodimer, the major telomere-specific single-stranded DNA-binding protein in mammalian cells, protects chromosome ends and contributes to the regulation of telomerase. The recent discovery of telomeric RNA raises the question of how POT1 faithfully binds telomeric ssDNA and avoids illicit RNA binding ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nandakumar J,Podell ER,Cech TR

更新日期：2010-01-12 00:00:00

abstract：:The molecular mechanism of tolerance to opiate drugs is poorly understood. We have used single-channel patch-clamp recordings to study opiate receptor effects on dissociated neurons from rat amygdala, a limbic region implicated in addiction processes. A 130-pS inwardly rectifying K(+)-preferring cation channel was act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Marrero HG,Murphy R,Lin YJ,Freedman JE

更新日期：2000-12-19 00:00:00

abstract：:Noniridescent coloration by the spongy keratin in parrot feather barbs has fascinated scientists. Nonetheless, its ultimate origin remains as yet unanswered, and a quantitative structural and optical description is still lacking. Here we report on structural and optical characterizations and numerical simulations of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yin H,Dong B,Liu X,Zhan T,Shi L,Zi J,Yablonovitch E

更新日期：2012-07-03 00:00:00

abstract：:B-1a cells, an anatomically, phenotypically, and functionally distinct subset of B cells that produce the bulk of natural serum IgM and much of gut-associated IgA, are an important component of the early response to pathogens. Because the induced expression of CD5, a hallmark of B-1a cells, requires a nuclear factor o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berland R,Wortis HH

更新日期：2003-11-11 00:00:00

abstract：:The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skinner MM,Zhang H,Leschnitzer DH,Guan Y,Bellamy H,Sweet RM,Gray CW,Konings RN,Wang AH,Terwilliger TC

更新日期：1994-03-15 00:00:00

abstract：:Although the first observations of the inhibitory effect of methylation on gene activity were made almost a decade ago, the mechanism by which methyl groups affect transcription is still obscure. Here we use engineered promoters methylated in vitro in transient transfections to study the mechanism by which methylation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine A,Cantoni GL,Razin A

更新日期：1992-11-01 00:00:00

abstract：:Functional lysosomes mediate autophagy and macropinocytosis for nutrient acquisition. Pancreatic ductal adenocarcinoma (PDAC) tumors exhibit high basal lysosomal activity, and inhibition of lysosome function suppresses PDAC cell proliferation and tumor growth. However, the codependencies induced by lysosomal inhibitio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elliott IA,Dann AM,Xu S,Kim SS,Abt ER,Kim W,Poddar S,Moore A,Zhou L,Williams JL,Capri JR,Ghukasyan R,Matsumura C,Tucker DA,Armstrong WR,Cabebe AE,Wu N,Li L,Le TM,Radu CG,Donahue TR

更新日期：2019-04-02 00:00:00

abstract：:The effects of temperature and of the action of a purified phospholipase C enzyme preparation on human red blood cell membranes has been investigated by chemical analyses, circular dichroism, and proton magnetic resonance measurements. The results indicate that a substantial fraction of the phospholipids and the prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glaser M,Simpkins H,Singer SJ,Sheetz M,Chan SI

更新日期：1970-03-01 00:00:00

abstract：:Hereditary deficiency of factor IXa (fIXa), a key enzyme in blood coagulation, causes hemophilia B, a severe X chromosome-linked bleeding disorder afflicting 1 in 30,000 males; clinical studies have identified nearly 500 deleterious variants. The x-ray structure of porcine fIXa described here shows the atomic origins ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brandstetter H,Bauer M,Huber R,Lollar P,Bode W

更新日期：1995-10-10 00:00:00

abstract：:The tightly coupled nature of the reaction sequence catalyzed by monoterpene cyclases has precluded direct observation of the topologically required isomerization step leading from geranyl pyrophosphate to the presumptive, enzyme-bound, tertiary allylic intermediate linalyl pyrophosphate, which ultimately cyclizes to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wheeler CJ,Croteau RB

更新日期：1987-07-01 00:00:00

abstract：:Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response has rarely been studied. Here, we show that the mice immunized with a l-f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao SF,Liang CH,Ho MY,Hsu TL,Tsai TI,Hsieh YS,Tsai CM,Li ST,Cheng YY,Tsao SM,Lin TY,Lin ZY,Yang WB,Ren CT,Lin KI,Khoo KH,Lin CH,Hsu HY,Wu CY,Wong CH

更新日期：2013-08-20 00:00:00

abstract：:The archaeological record shows that typically human cultural traits emerged at different times, in different parts of the world, and among different hominin taxa. This pattern suggests that their emergence is the outcome of complex and nonlinear evolutionary trajectories, influenced by environmental, demographic, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： d'Errico F,Banks WE,Warren DL,Sgubin G,van Niekerk K,Henshilwood C,Daniau AL,Sánchez Goñi MF

更新日期：2017-07-25 00:00:00

abstract：:Adult naive T cells, which are at rest in normal conditions, proliferate strongly when transferred to lymphopenic hosts. In neonates, the first mature thymocytes to migrate to the periphery reach a compartment devoid of preexisting T cells. We have extensively analyzed the proliferation rate and phenotype of periphera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Campion A,Bourgeois C,Lambolez F,Martin B,Léaument S,Dautigny N,Tanchot C,Pénit C,Lucas B

更新日期：2002-04-02 00:00:00

abstract：:I describe some recent observations of large-scale structure in the galaxy distribution. The best constraints come from two-dimensional galaxy surveys and studies of angular correlation functions. Results from galaxy redshift surveys are much less precise but are consistent with the angular correlations, provided the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Efstathiou G

更新日期：1993-06-01 00:00:00

abstract：:Our previous work [Levinger, L. & Varshavsky, A. (1982) Cell 28, 375-385] has shown that D1, a 50-kilodalton chromosomal protein of Drosophila melanogaster, is specifically associated with isolated nucleosomes that contain a complex A + T-rich satellite DNA with buoyant density of 1.688 g/ml. We show here that D1 is a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levinger L,Varshavsky A

更新日期：1982-12-01 00:00:00

abstract：:By manufacturing a single-particle system in two particulate forms (i.e., micrometer size and nanometer size), we have designed a bacterial vaccine form that exhibits improved efficacy of immunization. Microstructural properties are adapted to alter dispersive and aerosol properties independently. Dried "nanomicropart...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garcia-Contreras L,Wong YL,Muttil P,Padilla D,Sadoff J,Derousse J,Germishuizen WA,Goonesekera S,Elbert K,Bloom BR,Miller R,Fourie PB,Hickey A,Edwards D

更新日期：2008-03-25 00:00:00

abstract：:The role of Notch signaling in cartilage differentiation and maturation in vivo was examined. Conditional Notch pathway gain and loss of function was achieved using a Cre/loxP approach to manipulate Notch signaling in cartilage precursors and chondrocytes of the developing mouse embryo. Conditional overexpression of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mead TJ,Yutzey KE

更新日期：2009-08-25 00:00:00

abstract：:We have cloned two species of EcoRI-cleaved DNA segments that together cover the entire sequence for the human interleukin 2 gene and have determined the nucleotide sequence of the gene and its flanking regions. The gene contains three introns and the exon sequences can be aligned with the previously reported cDNA seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita T,Takaoka C,Matsui H,Taniguchi T

更新日期：1983-12-01 00:00:00

abstract：:Frame-shift mutations at microsatellites occur as a time-dependent function of polymerase errors followed by failure of postreplicational mismatch repair. A cell-culture system was developed that allows identification of intermediate mutant cells that carry the mutation on a single DNA strand after the initial DNA pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gasche C,Chang CL,Natarajan L,Goel A,Rhees J,Young DJ,Arnold CN,Boland CR

更新日期：2003-02-18 00:00:00

abstract：:At meiotic prophase, chromatin loops around a proteinaceous core, with the sizes of these loops varying between species. Comparison of the morphology of sequence-related inserts at different sites in transgenic mice demonstrates that loop size also varies with chromosomal geography. Similarly, chromatin loop lengths d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heng HH,Chamberlain JW,Shi XM,Spyropoulos B,Tsui LC,Moens PB

更新日期：1996-04-02 00:00:00

abstract：:Ethylene inhibits hypocotyl elongation in etiolated Arabidopsis seedlings. However, when Arabidopsis was grown in the light in the presence of ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC), a marked induction of hypocotyl elongation occurred. This resulted from an increase in cell expansion rat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smalle J,Haegman M,Kurepa J,Van Montagu M,Straeten DV

更新日期：1997-03-18 00:00:00

abstract：:Most genetic regulatory mechanisms involve protein-DNA interactions. In these processes, the classical Watson-Crick DNA structure sometimes is distorted severely, which in turn enables the precise recognition of the specific sites by the protein. Despite its key importance, very little is known about such deformation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leger JF,Robert J,Bourdieu L,Chatenay D,Marko JF

更新日期：1998-10-13 00:00:00

abstract：:Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of inducing actin tail formation within the cytoplasm of macrophages, leading to actin-based motility and intercellular spread. Actin tail formation by M. marinum is markedly reduced in macrophages deficient in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamm LM,Pak MA,Morisaki JH,Snapper SB,Rottner K,Lommel S,Brown EJ

更新日期：2005-10-11 00:00:00

abstract：:The spatial coordination of growth is of central importance for the regulation of plant tissue architecture. Individual layers, such as the epidermis, are clonally propagated and structurally maintained by symmetric cell divisions that are oriented along the plane of the layer. The developmental control of this proces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enugutti B,Kirchhelle C,Oelschner M,Torres Ruiz RA,Schliebner I,Leister D,Schneitz K

更新日期：2012-09-11 00:00:00