Abstract:
:Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere-mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6-16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1.
journal_name
Nat Genetjournal_title
Nature geneticsauthors
Itzhaki JE,Barnett MA,MacCarthy AB,Buckle VJ,Brown WR,Porter ACdoi
10.1038/ng1292-283keywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
283-7issue
4eissn
1061-4036issn
1546-1718journal_volume
2pub_type
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