The growth inhibitory effect of p21 adenovirus on androgen-dependent and -independent human prostate cancer cells.

Abstract:

OBJECTIVE:To assess the potential of p21 as a gene therapy treatment for prostate cancer, by introducing p21 into both androgen-dependent (AD) and -independent (AI) human prostate cancer cell lines via a recombinant adenoviral vector, Ad5CMV-p21, carrying human p21 cDNA. MATERIALS AND METHODS:The LNCaP, DU145 and PC-3 human prostate cancer cell lines were cultured and infected with Ad5CMV-p21. Cell growth, cell-cycle progression and tumorigenicity were then assessed by thymidine incorporation into cellular DNA, and cell number, flow cytometry, and tumour growth after inoculating the cells into nude mice. RESULTS:Growth was inhibited in Ad5CMV-p21 viral-infected AD and AI prostate cancer cells. The effects were dose-dependent, regardless of the androgen status of the cell lines. Flow cytometric analysis showed that Ad5CMV-p21 arrested cell-cycle progression at G1/S with no appreciable effect on the levels of apoptotic cells. The tumorigenicity of cancer cells infected with Ad5CMV-p21 was greatly reduced in athymic mice. CONCLUSIONS:These results suggest that Ad5CMV-p21 may be a new therapeutic agent for human prostate cancer gene therapy.

journal_name

BJU Int

journal_title

BJU international

authors

Gotoh A,Shirakawa T,Wada Y,Fujisawa M,Okada H,Kamidono S,Hamada K

doi

10.1046/j.1464-410x.2003.04318.x

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

314-8

issue

3

eissn

1464-4096

issn

1464-410X

pii

4318

journal_volume

92

pub_type

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