Zirconyl hematoxylin vs. alcian blue staining in a case of extramammary Paget's disease.

Abstract:

BACKGROUND:Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials. METHODS:We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl hematoxylin. RESULTS:Each staining procedure stained the same cells. CONCLUSIONS:Zirconyl hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.

journal_name

J Cutan Pathol

authors

Smith AA,Silver TM

doi

10.1034/j.1600-0560.2003.00080.x

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

401-4

issue

6

eissn

0303-6987

issn

1600-0560

pii

080

journal_volume

30

pub_type

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