Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium.


BACKGROUND AND AIMS:Biliary lipid absorption by the gall bladder mucosa and the cholesterol content of the gall bladder wall appear to play a role in cholesterol gall stone formation. As the scavenger receptor class B type I (SR- BI) regulates cellular cholesterol uptake, we studied its expression in human and murine gall bladders, its regulation by increased biliary lipid content, and its role in gall stone formation. METHODS AND RESULTS:Using immunohistochemistry, SR-BI was found in the apical domain of human gall bladder epithelial cells. Immunoblotting of isolated membranes from gall bladder epithelial cells showed a specific signal for the 82 kDa SR-BI protein. In C57BL/6 mice, SR-BI was also found in the gall bladder epithelium. Using western blot analysis, an inverse relationship was observed between biliary cholesterol concentration and SR-BI expression in murine gall bladder mucosa. By comparing lithogenic diet fed wild-type and SR-BI deficient mice, gall bladder wall cholesterol content and gall stone formation were not found to be dependent on SR-BI expression. CONCLUSIONS:(i) SR-BI is expressed in both human and murine gall bladder epithelium; (ii) biliary cholesterol hypersecretion is associated with decreased gall bladder SR-BI expression in mice; and (iii) murine SR-BI is not essential in controlling gall bladder wall cholesterol content and gall stone formation during diet induced cholelithiasis.






Miquel JF,Moreno M,Amigo L,Molina H,Mardones P,Wistuba II,Rigotti A





Has Abstract


2003-07-01 00:00:00














  • U.K. guidelines on the management of variceal haemorrhage in cirrhotic patients.

    abstract::These updated guidelines on the management of variceal haemorrhage have been commissioned by the Clinical Services and Standards Committee (CSSC) of the British Society of Gastroenterology (BSG) under the auspices of the liver section of the BSG. The original guidelines which this document supersedes were written in 2...


    pub_type: 杂志文章


    authors: Tripathi D,Stanley AJ,Hayes PC,Patch D,Millson C,Mehrzad H,Austin A,Ferguson JW,Olliff SP,Hudson M,Christie JM,Clinical Services and Standards Committee of the British Society of Gastroenterology.

    更新日期:2015-11-01 00:00:00

  • Transit through the gut measured by analysis of a single stool.

    abstract::A method is described for measuring transit time through the gut which requires the collection of only one stool. A dose of 20 radio-opaque markers is given to the subject on each of three consecutive days with breakfast, each dose of marker being of a different type. The first stool passed after rising on the fourth ...


    pub_type: 杂志文章


    authors: Cummings JH,Wiggins HS

    更新日期:1976-03-01 00:00:00

  • Five-year risk for advanced colorectal neoplasia after initial colonoscopy according to the baseline risk stratification: a prospective study in 2452 asymptomatic Koreans.

    abstract:OBJECTIVE:Postpolypectomy surveillance guidelines for colorectal cancer introduced the concept of 'risk stratification'; however, few studies have been conducted for validation of its usefulness. The aim of this study was to assess the 5-year incidence of advanced neoplasia recurrence based on the risk stratification s...


    pub_type: 杂志文章


    authors: Chung SJ,Kim YS,Yang SY,Song JH,Kim D,Park MJ,Kim SG,Song IS,Kim JS

    更新日期:2011-11-01 00:00:00

  • Cerebral processing of painful oesophageal stimulation: a study based on independent component analysis of the EEG.

    abstract:BACKGROUND AND AIMS:Independent component analysis (ICA) of the electroencephalogram (EEG) overcomes many of the classical problems in EEG analysis. We used ICA to determine the brain responses to painful stimulation of the oesophagus. METHODS:Twelve subjects with a median age of 41 years were included. With a nasal e...


    pub_type: 临床试验,杂志文章


    authors: Drewes AM,Sami SA,Dimcevski G,Nielsen KD,Funch-Jensen P,Valeriani M,Arendt-Nielsen L

    更新日期:2006-05-01 00:00:00

  • Hypnotherapy in severe irritable bowel syndrome: further experience.

    abstract::Fifteen patients with severe intractable irritable bowel syndrome previously reported as successfully treated with hypnotherapy, have now been followed up for a mean duration of 18 months. All patients remain in remission although two have experienced a single relapse overcome by an additional session of hypnotherapy....


    pub_type: 杂志文章


    authors: Whorwell PJ,Prior A,Colgan SM

    更新日期:1987-04-01 00:00:00

  • Identifying and testing candidate genetic polymorphisms in the irritable bowel syndrome (IBS): association with TNFSF15 and TNFα.

    abstract:OBJECTIVES:The postinfectious irritable bowel syndrome (PI-IBS) suggests that impaired resolution of inflammation could cause IBS symptoms. The authors hypothesised that polymorphisms in genes whose expression were altered by gastroenteritis might be linked to IBS with diarrhoea (IBS-D) which closely resembles PI-IBS. ...


    pub_type: 杂志文章


    authors: Swan C,Duroudier NP,Campbell E,Zaitoun A,Hastings M,Dukes GE,Cox J,Kelly FM,Wilde J,Lennon MG,Neal KR,Whorwell PJ,Hall IP,Spiller RC

    更新日期:2013-07-01 00:00:00

  • Preparation of lymphoid cells from small specimens of human gastrointestinal mucosa.

    abstract::Several methods for the preparation of cell suspensions from human gastrointestinal mucosa were investigated. Satisfactory suspensions were obtained by incubating tissue fragments in a solution of collagenase and hyaluronidase overnight at 4 degrees C followed by 30 minutes at 37 degrees C. The resulting suspension co...


    pub_type: 杂志文章


    authors: Crofton RW,Cochrane C,McClelland DB

    更新日期:1978-10-01 00:00:00

  • Threefold increased risk of infertility: a meta-analysis of infertility after ileal pouch anal anastomosis in ulcerative colitis.

    abstract:BACKGROUND:Increased infertility in women has been reported after ileal pouch-anal anastomosis (IPAA) for ulcerative colitis but reported infertility rates vary substantially. AIMS:(1) To perform a systematic review and meta-analysis of the relative risk of infertility post-IPAA compared with medical management; (2) t...


    pub_type: 杂志文章,meta分析,评审


    authors: Waljee A,Waljee J,Morris AM,Higgins PD

    更新日期:2006-11-01 00:00:00

  • Shanghai fever: a distinct Pseudomonas aeruginosa enteric disease.

    abstract:BACKGROUND:Shanghai fever, a community-acquired enteric illness associated with sepsis caused by Pseudomonas aeruginosa, was first described in 1918. The understanding of Shanghai fever is incomplete. OBJECTIVE:To delineate the clinical features and to examine the host and microbial factors associated with Shanghai fe...


    pub_type: 杂志文章


    authors: Chuang CH,Wang YH,Chang HJ,Chen HL,Huang YC,Lin TY,Ozer EA,Allen JP,Hauser AR,Chiu CH

    更新日期:2014-05-01 00:00:00

  • Interval sampling of end-expiratory hydrogen (H2) concentrations to quantify carbohydrate malabsorption by means of lactulose standards.

    abstract::Lactulose H2 breath tests are widely used for quantifying carbohydrate malabsorption, but the validity of the commonly used technique (interval sampling of H2 concentrations) has not been systematically investigated. In eight healthy adults we studied the reproducibility of the technique and the accuracy with which 5 ...


    pub_type: 杂志文章


    authors: Rumessen JJ,Hamberg O,Gudmand-Høyer E

    更新日期:1990-01-01 00:00:00

  • Duodenal ulcer and refined carbohydrate intake: a case-control study assessing dietary fibre and refined sugar intake.

    abstract::An association between duodenal ulceration and a low fibre intake and a high refined carbohydrate diet has been reported. We therefore compared the current diet, smoking habits, social class, and possible other risk factors of 78 patients with duodenal ulcer and a community control group matched for age and sex. Logis...


    pub_type: 杂志文章


    authors: Katschinski BD,Logan RF,Edmond M,Langman MJ

    更新日期:1990-09-01 00:00:00

  • Protein kinase C inhibits cyclic adenosine monophosphate generation by histamine and truncated glucagon like peptide 1 in the human gastric cancer cell line HGT-1.

    abstract::The HGT-1 gastric cancer cell line was used to determine the actions of protein kinase C on the stimulation of adenylate cyclase by the human histamine H2 receptor, and the receptors for gastric inhibitory polypeptide and truncated glucagon like peptide 1 (TGLP-1). Suspensions of HGT-1 cells were preincubated with the...


    pub_type: 杂志文章


    authors: McKenna P,Williams JM,Gespach CP,Hanson PJ

    更新日期:1993-07-01 00:00:00

  • Intraepithelial lymphocyte mitosis in a jejunal biopsy correlates with intraepithelial lymphocyte count, irrespective of diagnosis.

    abstract::When there is villus atrophy in a jejunal biopsy, intraepithelial lymphocyte (IEL) mitosis correlates with a diagnosis of coeliac disease. We have examined the significance of IEL mitosis in jejunal biopsies with normal villi. Counts of IEL per 100 villus enterocytes, and IEL mitosis per 1000 IEL, were carried out in ...


    pub_type: 杂志文章


    authors: Ferguson A,Ziegler K

    更新日期:1986-06-01 00:00:00

  • Global prevalence of Barrett's oesophagus and oesophageal cancer in individuals with gastro-oesophageal reflux: a systematic review and meta-analysis.

    abstract:OBJECTIVES:Chronic gastro-oesophageal reflux might lead to the development of Barrett's oesophagus (BO) or even oesophageal adenocarcinoma. There has been no definitive systematic review and meta-analysis of data to estimate global prevalence of BO or oesophageal adenocarcinoma in individuals with gastro-oesophageal re...


    pub_type: 杂志文章


    authors: Eusebi LH,Cirota GG,Zagari RM,Ford AC

    更新日期:2020-07-30 00:00:00

  • Gut microbes define liver cancer risk in mice exposed to chemical and viral transgenic hepatocarcinogens.

    abstract:BACKGROUND AND AIMS:Hepatocellular carcinoma (HCC) frequently results from synergism between chemical and infectious liver carcinogens. Worldwide, the highest incidence of HCC is in regions endemic for the foodborne contaminant aflatoxin B1 (AFB1) and hepatitis B virus (HBV) infection. Recently, gut microbes have been ...


    pub_type: 杂志文章


    authors: Fox JG,Feng Y,Theve EJ,Raczynski AR,Fiala JL,Doernte AL,Williams M,McFaline JL,Essigmann JM,Schauer DB,Tannenbaum SR,Dedon PC,Weinman SA,Lemon SM,Fry RC,Rogers AB

    更新日期:2010-01-01 00:00:00

  • Hydrogen sulphide and the hyperdynamic circulation in cirrhosis: a hypothesis.

    abstract::Cirrhosis is associated with the development of a hyperdynamic circulation, which is secondary to the presence of systemic vasodilatation. Several mechanisms have been postulated to be involved in the development of systemic vasodilatation, including increased synthesis of nitric oxide, hyperglucagonaemia, increased c...


    pub_type: 杂志文章,评审


    authors: Ebrahimkhani MR,Mani AR,Moore K

    更新日期:2005-12-01 00:00:00

  • Cellular response to alpha-gliadin in untreated coeliac disease.

    abstract::An improved technique for the detection of alpha-gliadin sensitised mononuclear cells in the peripheral blood of untreated coeliac patients is described. This method is a modification of the direct LMIF assay, and involves exposure of lymphocytes to alpha-gliadin and the assay of the resultant lymphokine produced usin...


    pub_type: 杂志文章


    authors: O'Farrelly C,Feighery C,Greally JF,Weir DG

    更新日期:1982-01-01 00:00:00

  • An interim report on the production of colonic diverticula in the rabbit.

    abstract::Six New Zealand white rabbits were given gas induction anaesthetics and maintained on glucochloralose (Merck) 60 mg/kg given intravenously. Intracolonic pressures were measured after a standard dose of prostigmine, 0.05 mg/kg, using fluid-filled, open-tipped, narrow-bore polythene tubes. Then the rabbits were put on a...


    pub_type: 杂志文章


    authors: Hodgson WJ

    更新日期:1972-10-01 00:00:00

  • Cystic dystrophy of the gastric and duodenal wall developing in heterotopic pancreas: an unrecognised entity.

    abstract::Ten patients in whom cystic dystrophy developed in a heterotopic pancreas of the duodenal (nine patients) or gastric (one patient) wall are reported. All were young or middle aged white men, only two of whom were alcoholic. The symptoms were caused by intestinal or biliary stenosis, or both, secondary to the inflammat...


    pub_type: 杂志文章


    authors: Fléjou JF,Potet F,Molas G,Bernades P,Amouyal P,Fékété F

    更新日期:1993-03-01 00:00:00

  • Delta 4-3-oxosteroid 5 beta-reductase deficiency: failure of ursodeoxycholic acid treatment and response to chenodeoxycholic acid plus cholic acid.

    abstract:BACKGROUND:In some infants with liver disease, 3-oxo-delta 4 bile acids are the major bile acids in urine, a phenomenon attributed to reduced activity of the delta 4-3-oxosteroid 5 beta-reductase required for synthesis of chenodeoxycholic acid and cholic acid. These patients form a heterogeneous group. Many have a know...


    pub_type: 杂志文章


    authors: Clayton PT,Mills KA,Johnson AW,Barabino A,Marazzi MG

    更新日期:1996-04-01 00:00:00

  • microRNA overexpression in slow transit constipation leads to reduced NaV1.5 current and altered smooth muscle contractility.

    abstract:OBJECTIVE:This study was designed to evaluate the roles of microRNAs (miRNAs) in slow transit constipation (STC). DESIGN:All human tissue samples were from the muscularis externa of the colon. Expression of 372 miRNAs was examined in a discovery cohort of four patients with STC versus three age/sex-matched controls by...


    pub_type: 杂志文章


    authors: Mazzone A,Strege PR,Gibbons SJ,Alcaino C,Joshi V,Haak AJ,Tschumperlin DJ,Bernard CE,Cima RR,Larson DW,Chua HK,Graham RP,El Refaey M,Mohler PJ,Hayashi Y,Ordog T,Calder S,Du P,Farrugia G,Beyder A

    更新日期:2020-05-01 00:00:00

  • Hepatitis C virus: epidemiology and genotypes in the north east of England.

    abstract::The epidemiology of hepatitis C virus (HCV) infection was studied in an English teaching hospital over an 18 month period. A total of 104 HCV antibody positive patients were referred for further investigation. They were divided into those diagnosed through screening (blood donors and intravenous drug abusers) and thos...


    pub_type: 杂志文章


    authors: Watson JP,Brind AM,Chapman CE,Bates CL,Gould FK,Johnson SJ,Burt AD,Ferguson J,Simmonds P,Bassendine MF

    更新日期:1996-02-01 00:00:00

  • Pathophysiological role of secretory type I and II phospholipase A2 in acute pancreatitis: an experimental study in rats.

    abstract:BACKGROUND:In human acute pancreatitis two different types of secretory phospholipase A2 (PLA2) have been found. AIM:To analyse the specific pattern of distribution of these PLA2 activities and their pathophysiological role in experimental acute pancreatitis. SUBJECTS AND METHODS:Catalytic activities of secretory typ...


    pub_type: 杂志文章


    authors: Uhl W,Schrag HJ,Schmitter N,Nevalainen TJ,Aufenanger J,Wheatley AM,Büchler MW

    更新日期:1997-03-01 00:00:00

  • Effect of carbenoxolone on the synthesis of glycoproteins and DNA in rat gastric epithelial cells.

    abstract::The influence of carbenoxolone on the synthesis of glycoproteins in the surface mucous cells and the production of new cells in the rat gastric mucosa was studied by means of a vascular perfusion system. The rate of incorporation of tritiated galactose, glucosamine, serine, and sulphate in surface mucous cells, studie...


    pub_type: 杂志文章


    authors: van Huis GA,Kramer MF

    更新日期:1981-10-01 00:00:00

  • The sequential therapy regimen for Helicobacter pylori eradication: a pooled-data analysis.

    abstract:BACKGROUND:Standard triple therapies are the most used treatment in clinical practice. However, a critical fall in the H pylori eradication rate following these therapies has been observed in the last few years. The sequential regimen is a novel, promising therapeutic approach. OBJECTIVES:To evaluate the available dat...


    pub_type: 杂志文章,meta分析,评审


    authors: Zullo A,De Francesco V,Hassan C,Morini S,Vaira D

    更新日期:2007-10-01 00:00:00

  • NAD metabolism fuels human and mouse intestinal inflammation.

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    pub_type: 杂志文章


    authors: Gerner RR,Klepsch V,Macheiner S,Arnhard K,Adolph TE,Grander C,Wieser V,Pfister A,Moser P,Hermann-Kleiter N,Baier G,Oberacher H,Tilg H,Moschen AR

    更新日期:2018-10-01 00:00:00

  • PPARgamma as a new therapeutic target in inflammatory bowel diseases.

    abstract::The peroxisome proliferator activated receptor gamma(PPARgamma) is a nuclear receptor highly expressed in the colon and playing a key role in bacterial induced inflammation. Regulation of colon inflammation by this receptor has been well demonstrated in many experimental models of colitis but also in patients with ulc...


    pub_type: 杂志文章,评审


    authors: Dubuquoy L,Rousseaux C,Thuru X,Peyrin-Biroulet L,Romano O,Chavatte P,Chamaillard M,Desreumaux P

    更新日期:2006-09-01 00:00:00

  • Determinants of perception of heartburn and regurgitation.

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    pub_type: 杂志文章


    authors: Bredenoord AJ,Weusten BL,Curvers WL,Timmer R,Smout AJ

    更新日期:2006-03-01 00:00:00

  • Gut microbiome analysis as a tool towards targeted non-invasive biomarkers for early hepatocellular carcinoma.

    abstract:OBJECTIVE:To characterise gut microbiome in patients with hepatocellular carcinoma (HCC) and evaluate the potential of microbiome as non-invasive biomarkers for HCC. DESIGN:We collected 486 faecal samples from East China, Central China and Northwest China prospectively and finally 419 samples completed Miseq sequencin...


    pub_type: 杂志文章


    authors: Ren Z,Li A,Jiang J,Zhou L,Yu Z,Lu H,Xie H,Chen X,Shao L,Zhang R,Xu S,Zhang H,Cui G,Chen X,Sun R,Wen H,Lerut JP,Kan Q,Li L,Zheng S

    更新日期:2019-06-01 00:00:00

  • Altered migrating myoelectrical complex in an animal model of cholesterol gallstone disease: the effect of erythromycin.

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    pub_type: 杂志文章


    authors: Xu QW,Scott RB,Tan DT,Shaffer EA

    更新日期:1998-12-01 00:00:00