Abstract:
:We have devised a class of isothermal reactions for amplifying DNA. These homogeneous reactions rapidly synthesize short oligonucleotides (8-16 bases) specified by the sequence of an amplification template. Versions of the reactions can proceed in either a linear or an exponential amplification mode. Both of these reactions require simple, constant conditions, and the rate of amplification depends entirely on the molecular parameters governing the interactions of the molecules in the reaction. The exponential version of the reaction is a molecular chain reaction that uses the oligonucleotide products of each linear reaction to create producers of more of the same oligonucleotide. It is a highly sensitive chain reaction that can be specifically triggered by given DNA sequences and can achieve amplifications of >10(6)-fold. Several similar reactions in this class are described here. The robustness, speed, and sensitivity of the exponential reaction suggest it will be useful in rapidly detecting the presence of small amounts of a specific DNA sequence in a sample, and a range of other applications, including many currently making use of the PCR.
journal_name
Proc Natl Acad Sci U S Aauthors
Van Ness J,Van Ness LK,Galas DJdoi
10.1073/pnas.0730811100keywords:
subject
Has Abstractpub_date
2003-04-15 00:00:00pages
4504-9issue
8eissn
0027-8424issn
1091-6490pii
0730811100journal_volume
100pub_type
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