Abstract:
:In human embryos, blastomeres differentiate into trophectoderm (TE) cells and inner cell mass (ICM) cells of blastocysts. Although morphologically indistinguishable, blastomeres at early cleavage stages are likely to undergo changes on a molecular level that make them destined to become ICM or TE cells. While the transcription factor Oct-4 might serve as a marker for totipotent ICM cells, human chorionic gonadotrophin might be used as the equivalent for TE cells. This study reports a reverse transcription-polymerase chain reaction procedure to assess human beta-HCG mRNA concentrations as well as ploidy in individual blastomeres from normally and abnormally fertilized human embryos. beta-HCG mRNA was detected in both euploid and aneuploid cells and in oocytes. Surprisingly, beta-LH mRNA was also detected in some euploid blastomeres. In regard to preimplantation genetic diagnosis, assessment of expression levels of beta-HCG and Oct-4 mRNA in individual biopsied cells might serve as a tool to identify embryogenic blastomeres in combination with testing for chromosome and single gene abnormalities.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Hansis C,Grifo JA,Tang Y,Krey LCdoi
10.1016/s1472-6483(10)61618-5keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
156-61issue
2eissn
1472-6483issn
1472-6491pii
S1472-6483(10)61618-5journal_volume
5pub_type
杂志文章abstract::The predictive value of pre-ovulatory hormones for achievement of pregnancy has been extensively evaluated in assisted reproductive technologies. Currently, no high-quality evidence exists to support or deny an association between oestradiol levels on the day of human chorionic gonadotrophin (HCG) administration and p...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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更新日期:2009-01-01 00:00:00
abstract:RESEARCH QUESTION:What is the relationship between the vaginal microbiota profile at the time of embryo transfer and live birth rates in women undergoing IVF/intracytoplasmic sperm injection (ICSI) with donated oocytes? DESIGN:One hundred and fifty Caucasian women receiving donated oocytes were prospectively included ...
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journal_title:Reproductive biomedicine online
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更新日期:2004-12-01 00:00:00
abstract::This study aims to compare a psychological evaluation test to classical psychoanalysis in infertile women. Two hundred women were submitted to the Psychological Evaluation Test (PET). The sum of the scores for the responses ranged from 15 to 60 points, with scores >/=30 points being defined as 'psycho-emotional maladj...
journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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abstract:RESEARCH QUESTION:Can single-nucleotide polymorphisms (SNP) of genes related to progesterone synthesis predict the risk of premature serum progesterone elevation in women undergoing gonadotrophin-releasing hormone agonist protocols for ovarian stimulation? DESIGN:A total of 765 women were divided into high progesteron...
journal_title:Reproductive biomedicine online
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更新日期:2020-03-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)62085-8
更新日期:2003-12-01 00:00:00
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journal_title:Reproductive biomedicine online
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更新日期:2004-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
abstract::The main adverse effect of IVF is the high multiple pregnancy rate resulting from the transfer of two or more embryos. The objective was to evaluate pregnancy rates in infertile women with a good prognosis who failed to conceive in a fresh elective single embryo transfer (eSET) and had a second cycle with elective dou...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,多中心研究
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更新日期:2005-12-01 00:00:00
abstract::A cohort follow-up study was designed to compare the efficacy of IVF using frozen donor semen (IVF-D) following previously failed DI cycles (unexplained female infertility) and direct IVF-D treatment because of tubal infertility (control group). The cohort comprised 189 couples initiating IVF-D after previously failed...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61275-8
更新日期:2004-10-01 00:00:00
abstract::IVF productivity rate is an index defined as the sum of all live births from either fresh or frozen embryo transfers arising from a single oocyte collection. This retrospective analysis over 9 continuous years used this index to understand the potential impact on pregnancy rates of milder stimulation regimens with ass...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2013.05.015
更新日期:2013-09-01 00:00:00
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journal_title:Reproductive biomedicine online
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doi:10.1016/j.rbmo.2020.09.012
更新日期:2021-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(12)60111-4
更新日期:2002-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60506-8
更新日期:2004-01-01 00:00:00
abstract::Multiple pregnancies are a serious adverse event of IVF treatment. Several strategies have been developed to improve embryo selection and embryo developmental potential to increase pregnancy rates without increasing the risk of multiple pregnancies. The only way to effectively solve this problem is to reduce the numbe...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/S1472-6483(10)61459-9
更新日期:2007-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.10.007
更新日期:2019-02-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)62217-1
更新日期:2005-03-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
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更新日期:2004-12-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60694-3
更新日期:2007-07-01 00:00:00
abstract::Tubal pathology is a common cause of subfertility. Identifying risk factors for tubal pathology in the medical history is important to distinguish between those couples who benefit from early tubal patency tests and those in whom presence of tubal pathology is less likely and delaying tubal tests is justified. This st...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60589-5
更新日期:2008-02-01 00:00:00
abstract::Gonadotrophin-releasing hormone (GnRH) agonists and antagonists have been widely used to prevent premature LH surge during ovarian stimulation. However, studies have shown a significantly lower serum oestradiol concentration on the day of human chorionic gonadotrophin administration for cycles using GnRH antagonist. T...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
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更新日期:2010-07-01 00:00:00
abstract::This study aimed to investigate whether the spermatozoa-zona pellucida binding test is able to select spermatozoa with higher fertilization potential and higher rate of successful embryo development. This prospective study was performed with metaphase II (MII) oocytes retrieved from couples undergoing intracytoplasmic...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2009.09.020
更新日期:2009-12-01 00:00:00
abstract::In spite of several past attempts at defining the point at which conception can be considered completed, resulting in the formation of an embryo, the existing definitions are still contradictory. In the absence of clear terminology, the application of laws aimed at the protection of early human life may have inadequat...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)62103-7
更新日期:2004-07-01 00:00:00
abstract::Spermatogenesis is a critical process for maintaining male fertility. Sustained spermatogonial stem cell self-renewal and differentiation ensures constant spermatogenesis, and several signalling pathways regulate this process. An increasing number of studies have suggested that the mammalian target of rapamycin (mTOR)...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2015.11.007
更新日期:2016-02-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is associated with increased risk of impaired glucose tolerance and type 2 diabetes. PCOS has been considered as a major risk factor for the development of diabetes, and screening of women with PCOS for glucose intolerance is suggested. Detection of glucose intolerance in PCOS is best ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(10)61645-8
更新日期:2004-06-01 00:00:00
abstract::This paper reports on and discusses the findings of an online survey initiated by the Donor Sibling Registry of 108 parents of children conceived following oocyte donation. Respondents generally supported early disclosure of donor conception to the child, although some bias in favour of disclosure cannot be excluded, ...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2012.10.013
更新日期:2013-02-01 00:00:00
abstract::This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological evaluation of mouse and human ...
journal_title:Reproductive biomedicine online
pub_type: 历史文章,杂志文章
doi:10.1016/j.rbmo.2010.10.015
更新日期:2011-02-01 00:00:00