当前位置： SCI文献检索 > NATURE MEDICINE期刊下所有文献 > Automated subcellular localization and quantification of protein expression in tissue microarrays.

Automated subcellular localization and quantification of protein expression in tissue microarrays.

Abstract：

:The recent development of tissue microarrays-composed of hundreds of tissue sections from different tumors arrayed on a single glass slide-facilitates rapid evaluation of large-scale outcome studies. Realization of this potential depends on the ability to rapidly and precisely quantify the protein expression within each tissue spot. We have developed a set of algorithms that allow the rapid, automated, continuous and quantitative analysis of tissue microarrays, including the separation of tumor from stromal elements and the sub-cellular localization of signals. Validation studies using estrogen receptor in breast carcinoma show that automated analysis matches or exceeds the results of conventional pathologist-based scoring. Automated analysis and sub-cellular localization of beta-catenin in colon cancer identifies two novel, prognostically significant tumor subsets, not detected by traditional pathologist-based scoring. Development of automated analysis technology empowers tissue microarrays for use in discovery-type experiments (more typical of cDNA microarrays), with the added advantage of inclusion of long-term demographic and patient outcome information.

journal_name

Nat Med

journal_title

Nature medicine

authors

Camp RL,Chung GG,Rimm DL

doi

10.1038/nm791

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

1323-7

issue

11

eissn

1078-8956

issn

1546-170X

pii

nm791

journal_volume

8

pub_type

杂志文章

相关文献

NATURE MEDICINE文献大全
  • Restoration of the tumor suppressor function to mutant p53 by a low-molecular-weight compound.

    abstract：:The tumor suppressor p53 inhibits tumor growth primarily through its ability to induce apoptosis. Mutations in p53 occur in at least 50% of human tumors. We hypothesized that reactivation of mutant p53 in such tumors should trigger massive apoptosis and eliminate the tumor cells. To test this, we screened a library of...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0302-282

    authors： Bykov VJ,Issaeva N,Shilov A,Hultcrantz M,Pugacheva E,Chumakov P,Bergman J,Wiman KG,Selivanova G

    更新日期：2002-03-01 00:00:00

  • Nogo-B regulates endothelial sphingolipid homeostasis to control vascular function and blood pressure.

    abstract：:Endothelial dysfunction is a critical factor in many cardiovascular diseases, including hypertension. Although lipid signaling has been implicated in endothelial dysfunction and cardiovascular disease, specific molecular mechanisms are poorly understood. Here we report that Nogo-B, a membrane protein of the endoplasmi...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3934

    authors： Cantalupo A,Zhang Y,Kothiya M,Galvani S,Obinata H,Bucci M,Giordano FJ,Jiang XC,Hla T,Di Lorenzo A

    更新日期：2015-09-01 00:00:00

  • Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.

    abstract：:Low oxygen tension influences tumor progression by enhancing angiogenesis; and histone deacetylases (HDAC) are implicated in alteration of chromatin assembly and tumorigenesis. Here we show induction of HDAC under hypoxia and elucidate a role for HDAC in the regulation of hypoxia-induced angiogenesis. Overexpressed wi...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/86507

    authors： Kim MS,Kwon HJ,Lee YM,Baek JH,Jang JE,Lee SW,Moon EJ,Kim HS,Lee SK,Chung HY,Kim CW,Kim KW

    更新日期：2001-04-01 00:00:00

  • Locoregional delivery of CAR T cells to the cerebrospinal fluid for treatment of metastatic medulloblastoma and ependymoma.

    abstract：:Recurrent medulloblastoma and ependymoma are universally lethal, with no approved targeted therapies and few candidates presently under clinical evaluation. Nearly all recurrent medulloblastomas and posterior fossa group A (PFA) ependymomas are located adjacent to and bathed by the cerebrospinal fluid, presenting an o...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/s41591-020-0827-2

    authors： Donovan LK,Delaidelli A,Joseph SK,Bielamowicz K,Fousek K,Holgado BL,Manno A,Srikanthan D,Gad AZ,Van Ommeren R,Przelicki D,Richman C,Ramaswamy V,Daniels C,Pallota JG,Douglas T,Joynt ACM,Haapasalo J,Nor C,Vladoiu MC,

    更新日期：2020-05-01 00:00:00

  • Antiviral pressure exerted by HIV-1-specific cytotoxic T lymphocytes (CTLs) during primary infection demonstrated by rapid selection of CTL escape virus.

    abstract：:The HIV-1-specific cytotoxic T lymphocyte (CTL) response is temporally associated with the decline in viremia during primary HIV-1 infection, but definitive evidence that it is of importance in virus containment has been lacking. Here we show that in a patient whose early CTL response was focused on a highly immunodom...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0297-205

    authors： Borrow P,Lewicki H,Wei X,Horwitz MS,Peffer N,Meyers H,Nelson JA,Gairin JE,Hahn BH,Oldstone MB,Shaw GM

    更新日期：1997-02-01 00:00:00

  • CD16 promotes Escherichia coli sepsis through an FcR gamma inhibitory pathway that prevents phagocytosis and facilitates inflammation.

    abstract：:Sepsis, a leading cause of death worldwide, involves proinflammatory responses and inefficient bacterial clearance. Phagocytic cells play a crucial part in the prevention of sepsis by clearing bacteria through host innate receptors. Here we show that the FcRgamma adaptor, an immunoreceptor tyrosine-based activation mo...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1665

    authors： Pinheiro da Silva F,Aloulou M,Skurnik D,Benhamou M,Andremont A,Velasco IT,Chiamolera M,Verbeek JS,Launay P,Monteiro RC

    更新日期：2007-11-01 00:00:00

  • Metagenomic and metabolomic analyses reveal distinct stage-specific phenotypes of the gut microbiota in colorectal cancer.

    abstract：:In most cases of sporadic colorectal cancers, tumorigenesis is a multistep process, involving genomic alterations in parallel with morphologic changes. In addition, accumulating evidence suggests that the human gut microbiome is linked to the development of colorectal cancer. Here we performed fecal metagenomic and me...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/s41591-019-0458-7

    authors： Yachida S,Mizutani S,Shiroma H,Shiba S,Nakajima T,Sakamoto T,Watanabe H,Masuda K,Nishimoto Y,Kubo M,Hosoda F,Rokutan H,Matsumoto M,Takamaru H,Yamada M,Matsuda T,Iwasaki M,Yamaji T,Yachida T,Soga T,Kurokawa K,Toy

    更新日期：2019-06-01 00:00:00

  • Effective and selective targeting of leukemia cells using a TORC1/2 kinase inhibitor.

    abstract：:Targeting the mammalian target of rapamycin (mTOR) protein is a promising strategy for cancer therapy. The mTOR kinase functions in two complexes, TORC1 (target of rapamycin complex-1) and TORC2 (target of rapamycin complex-2); however, neither of these complexes is fully inhibited by the allosteric inhibitor rapamyci...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.2091

    authors： Janes MR,Limon JJ,So L,Chen J,Lim RJ,Chavez MA,Vu C,Lilly MB,Mallya S,Ong ST,Konopleva M,Martin MB,Ren P,Liu Y,Rommel C,Fruman DA

    更新日期：2010-02-01 00:00:00

  • Mechanisms of NAFLD development and therapeutic strategies.

    abstract：:There has been a rise in the prevalence of nonalcoholic fatty liver disease (NAFLD), paralleling a worldwide increase in diabetes and metabolic syndrome. NAFLD, a continuum of liver abnormalities from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), has a variable course but can lead to cirrhosi...

    journal_title：Nature medicine

    pub_type： 杂志文章,评审

    doi：10.1038/s41591-018-0104-9

    authors： Friedman SL,Neuschwander-Tetri BA,Rinella M,Sanyal AJ

    更新日期：2018-07-01 00:00:00

  • Serum levels of the iron binding protein p97 are elevated in Alzheimer's disease.

    abstract：:Alzheimer's disease is a progressive and incurable disease whose prevalence increases dramatically with age. A biochemical marker for monitoring the onset and progression of the disease would be a valuable tool for disease management. In addition, such a marker might be used as an end point in clinical intervention pr...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1196-1230

    authors： Kennard ML,Feldman H,Yamada T,Jefferies WA

    更新日期：1996-11-01 00:00:00

  • A chimeric human-cat fusion protein blocks cat-induced allergy.

    abstract：:Animal allergens are an important cause of asthma and allergic rhinitis. We designed and tested a chimeric human-cat fusion protein composed of a truncated human IgG Fcgamma1 and the major cat allergen Fel d1, as a proof of concept for a new approach to allergy immunotherapy. This Fcgamma-Fel d1 protein induced dose-d...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1219

    authors： Zhu D,Kepley CL,Zhang K,Terada T,Yamada T,Saxon A

    更新日期：2005-04-01 00:00:00

  • Long-term correction of canine hemophilia B by gene transfer of blood coagulation factor IX mediated by adeno-associated viral vector.

    abstract：:Hemophilia B is a severe X-linked bleeding diathesis caused by the absence of functional blood coagulation factor IX, and is an excellent candidate for treatment of a genetic disease by gene therapy. Using an adeno-associated viral vector, we demonstrate sustained expression (>17 months) of factor IX in a large-animal...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/4743

    authors： Herzog RW,Yang EY,Couto LB,Hagstrom JN,Elwell D,Fields PA,Burton M,Bellinger DA,Read MS,Brinkhous KM,Podsakoff GM,Nichols TC,Kurtzman GJ,High KA

    更新日期：1999-01-01 00:00:00

  • Preventing dysbiosis of the neonatal mouse intestinal microbiome protects against late-onset sepsis.

    abstract：:Late-onset sepsis (LOS) is thought to result from systemic spread of commensal microbes from the intestines of premature infants. Clinical use of probiotics for LOS prophylaxis has varied owing to limited efficacy, reflecting an incomplete understanding of relationships between development of the intestinal microbiome...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/s41591-019-0640-y

    authors： Singer JR,Blosser EG,Zindl CL,Silberger DJ,Conlan S,Laufer VA,DiToro D,Deming C,Kumar R,Morrow CD,Segre JA,Gray MJ,Randolph DA,Weaver CT

    更新日期：2019-11-01 00:00:00

  • Gene expression-based survival prediction in lung adenocarcinoma: a multi-site, blinded validation study.

    abstract：:Although prognostic gene expression signatures for survival in early-stage lung cancer have been proposed, for clinical application, it is critical to establish their performance across different subject populations and in different laboratories. Here we report a large, training-testing, multi-site, blinded validation...

    journal_title：Nature medicine

    pub_type： 杂志文章,多中心研究

    doi：10.1038/nm.1790

    authors： Director's Challenge Consortium for the Molecular Classification of Lung Adenocarcinoma.,Shedden K,Taylor JM,Enkemann SA,Tsao MS,Yeatman TJ,Gerald WL,Eschrich S,Jurisica I,Giordano TJ,Misek DE,Chang AC,Zhu CQ,Strumpf D,Hanash

    更新日期：2008-08-01 00:00:00

  • A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects.

    abstract：:The adoptive transfer of T cells engineered with a chimeric antigen receptor (CAR) (hereafter referred to as CAR-T cells) specific for the B lymphocyte antigen CD19 has shown impressive clinical responses in patients with refractory B cell malignancies. However, the therapeutic effects of CAR-T cells that target other...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4478

    authors： Kagoya Y,Tanaka S,Guo T,Anczurowski M,Wang CH,Saso K,Butler MO,Minden MD,Hirano N

    更新日期：2018-03-01 00:00:00

  • Mutations in G protein β subunits promote transformation and kinase inhibitor resistance.

    abstract：:Activating mutations in genes encoding G protein α (Gα) subunits occur in 4-5% of all human cancers, but oncogenic alterations in Gβ subunits have not been defined. Here we demonstrate that recurrent mutations in the Gβ proteins GNB1 and GNB2 confer cytokine-independent growth and activate canonical G protein signalin...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3751

    authors： Yoda A,Adelmant G,Tamburini J,Chapuy B,Shindoh N,Yoda Y,Weigert O,Kopp N,Wu SC,Kim SS,Liu H,Tivey T,Christie AL,Elpek KG,Card J,Gritsman K,Gotlib J,Deininger MW,Makishima H,Turley SJ,Javidi-Sharifi N,Maciejewski

    更新日期：2015-01-01 00:00:00

  • The clock gene Per2 influences the glutamatergic system and modulates alcohol consumption.

    abstract：:Period (Per) genes are involved in regulation of the circadian clock and are thought to modulate several brain functions. We demonstrate that Per2(Brdm1) mutant mice, which have a deletion in the PAS domain of the Per2 protein, show alterations in the glutamatergic system. Lowered expression of the glutamate transport...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1163

    authors： Spanagel R,Pendyala G,Abarca C,Zghoul T,Sanchis-Segura C,Magnone MC,Lascorz J,Depner M,Holzberg D,Soyka M,Schreiber S,Matsuda F,Lathrop M,Schumann G,Albrecht U

    更新日期：2005-01-01 00:00:00

  • Inhibition of telomerase limits the growth of human cancer cells.

    abstract：:Telomerase is a ribonucleoprotein enzyme that maintains the protective structures at the ends of eukaryotic chromosomes, called telomeres. In most human somatic cells, telomerase expression is repressed, and telomeres shorten progressively with each cell division. In contrast, most human tumors express telomerase, res...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/13495

    authors： Hahn WC,Stewart SA,Brooks MW,York SG,Eaton E,Kurachi A,Beijersbergen RL,Knoll JH,Meyerson M,Weinberg RA

    更新日期：1999-10-01 00:00:00

  • The association between sterilizing activity and drug distribution into tuberculosis lesions.

    abstract：:Finding new treatment-shortening antibiotics to improve cure rates and curb the alarming emergence of drug resistance is the major objective of tuberculosis (TB) drug development. Using a matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging suite in a biosafety containment facility, we show th...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3937

    authors： Prideaux B,Via LE,Zimmerman MD,Eum S,Sarathy J,O'Brien P,Chen C,Kaya F,Weiner DM,Chen PY,Song T,Lee M,Shim TS,Cho JS,Kim W,Cho SN,Olivier KN,Barry CE 3rd,Dartois V

    更新日期：2015-10-01 00:00:00

  • Oral immunization with an anti-idiotypic antibody to the exoglycolipid antigen protects against experimental Chlamydia trachomatis infection.

    abstract：:Chlamydia trachomatis is the leading cause worldwide of preventable infectious blindness (trachoma) and sexually transmitted disease, including nongonoccocal urethritis and pelvic inflammatory disease. To date, no effective vaccine against C. trachomatis infection has been identified. A monoclonal anti-idiotypic antib...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1096-1116

    authors： Whittum-Hudson JA,An LL,Saltzman WM,Prendergast RA,MacDonald AB

    更新日期：1996-10-01 00:00:00

  • Re-engineered CD40 receptor enables potent pharmacological activation of dendritic-cell cancer vaccines in vivo.

    abstract：:Modest clinical outcomes of dendritic-cell (DC) vaccine trials call for the refinement of DC vaccine design. Although many potential antigens have been identified, development of methods to enhance antigen presentation by DCs has lagged. We have engineered a potent, drug-inducible CD40 (iCD40) receptor that permits te...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1183

    authors： Hanks BA,Jiang J,Singh RA,Song W,Barry M,Huls MH,Slawin KM,Spencer DM

    更新日期：2005-02-01 00:00:00

  • Transcriptional regulation of endochondral ossification by HIF-2alpha during skeletal growth and osteoarthritis development.

    abstract：:Chondrocyte hypertrophy followed by cartilage matrix degradation and vascular invasion, characterized by expression of type X collagen (COL10A1), matrix metalloproteinase-13 (MMP-13) and vascular endothelial growth factor (VEGF), respectively, are central steps of endochondral ossification during normal skeletal growt...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.2146

    authors： Saito T,Fukai A,Mabuchi A,Ikeda T,Yano F,Ohba S,Nishida N,Akune T,Yoshimura N,Nakagawa T,Nakamura K,Tokunaga K,Chung UI,Kawaguchi H

    更新日期：2010-06-01 00:00:00

  • Toll-like receptor 2-dependent induction of vitamin A-metabolizing enzymes in dendritic cells promotes T regulatory responses and inhibits autoimmunity.

    abstract：:Immune sensing of a microbe occurs via multiple receptors. How signals from different receptors are coordinated to yield a specific immune response is poorly understood. We show that two pathogen recognition receptors, Toll-like receptor 2 (TLR2) and dectin-1, recognizing the same microbial stimulus, stimulate distinc...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.1925

    authors： Manicassamy S,Ravindran R,Deng J,Oluoch H,Denning TL,Kasturi SP,Rosenthal KM,Evavold BD,Pulendran B

    更新日期：2009-04-01 00:00:00

  • Hypomorphic promoter mutation in PIGM causes inherited glycosylphosphatidylinositol deficiency.

    abstract：:Attachment to the plasma membrane by linkage to a glycosylphosphatidylinositol (GPI) anchor is a mode of protein expression highly conserved from protozoa to mammals. As a clinical entity, deficiency of GPI has been recognized as paroxysmal nocturnal hemoglobinuria, an acquired clonal disorder associated with somatic ...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1410

    authors： Almeida AM,Murakami Y,Layton DM,Hillmen P,Sellick GS,Maeda Y,Richards S,Patterson S,Kotsianidis I,Mollica L,Crawford DH,Baker A,Ferguson M,Roberts I,Houlston R,Kinoshita T,Karadimitris A

    更新日期：2006-07-01 00:00:00

  • Functional genomics of Neisseria meningitidis pathogenesis.

    abstract：:The pathogenic bacterium Neisseria meningitidis is an important cause of septicemia and meningitis, especially in childhood. The establishment and maintenance of bacteremic infection is a pre-requisite for all the pathological sequelae of meningococcal infection. To further understand the genetic basis of this essenti...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/81380

    authors： Sun YH,Bakshi S,Chalmers R,Tang CM

    更新日期：2000-11-01 00:00:00

  • Inhibition of fatty acid oxidation as a therapy for MYC-overexpressing triple-negative breast cancer.

    abstract：:Expression of the oncogenic transcription factor MYC is disproportionately elevated in triple-negative breast cancer (TNBC), as compared to estrogen receptor-, progesterone receptor- or human epidermal growth factor 2 receptor-positive (RP) breast cancer. We and others have shown that MYC alters metabolism during tumo...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4055

    authors： Camarda R,Zhou AY,Kohnz RA,Balakrishnan S,Mahieu C,Anderton B,Eyob H,Kajimura S,Tward A,Krings G,Nomura DK,Goga A

    更新日期：2016-04-01 00:00:00

  • Serum insulin-like growth factor I regulates brain amyloid-beta levels.

    abstract：:Levels of insulin-like growth factor I (IGF-I), a neuroprotective hormone, decrease in serum during aging, whereas amyloid-beta (Abeta), which is involved in the pathogenesis of Alzheimer disease, accumulates in the brain. High brain Abeta levels are found at an early age in mutant mice with low circulating IGF-I, and...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1202-793

    authors： Carro E,Trejo JL,Gomez-Isla T,LeRoith D,Torres-Aleman I

    更新日期：2002-12-01 00:00:00

  • A new penumbra: transitioning from injury into repair after stroke.

    abstract：:The penumbra is an area of brain tissue that is damaged but not yet dead after focal ischemia. The existence of a penumbra implies that therapeutic salvage is theoretically possible after stroke. The first decade of penumbral science investigated the ischemic regulation of electrophysiology, cerebral blood flow and me...

    journal_title：Nature medicine

    pub_type： 杂志文章,评审

    doi：10.1038/nm1735

    authors： Lo EH

    更新日期：2008-05-01 00:00:00

  • A combination of fluorescent NFAT and H2B sensors uncovers dynamics of T cell activation in real time during CNS autoimmunity.

    abstract：:Multiple sclerosis is an autoimmune disease of the central nervous system (CNS) that is initiated when self-reactive T cells enter the brain and become locally activated after encountering their specific nervous antigens. When and where the disease-relevant antigen encounters occur is unclear. Here we combined fluores...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3182

    authors： Lodygin D,Odoardi F,Schläger C,Körner H,Kitz A,Nosov M,van den Brandt J,Reichardt HM,Haberl M,Flügel A

    更新日期：2013-06-01 00:00:00

  • Vaccines: past, present and future.

    abstract：:The vaccines developed over the first two hundred years since Jenner's lifetime have accomplished striking reductions of infection and disease wherever applied. Pasteur's early approaches to vaccine development, attenuation and inactivation, are even now the two poles of vaccine technology. Today, purification of micr...

    journal_title：Nature medicine

    pub_type： 历史文章,杂志文章

    doi：10.1038/nm1209

    authors： Plotkin SA

    更新日期：2005-04-01 00:00:00