Pressure cell assisted solution characterization of polysaccharides. 2. Locust bean gum and tara gum.

Abstract:

:Following the work carried out on guar gum in our first paper of a series, the "pressure cell" solubilization method was applied to two other less highly substituted galactomannans: locust bean gum (LBG) and tara gum. True molecular solution of the polymers was achieved using appropriate temperature, time, and pressure regimes. The technique of capillary viscometry was used to determine the intrinsic viscosity [eta] of the "pressure cell" treated and untreated samples. Molecular weight (M(w)) and radius of gyration (R(g)) were determined by light scattering. The data obtained for LBG and tara gum were compared statistically with reliable data found for guar gum in the literature. The variation in [eta] with M(w) followed the Mark-Houwink-Sakurada relationship, giving the exponent alpha = 0.74 +/- 0.01 for galactomannans consistent with random coil behavior. The characteristic ratio, C(infinity), and the chain persistence length, L(p), were both calculated for LBG and tara gum using the Burchard-Stockmayer-Fixman (BSF) method which is appropriate for flexible to semiflexible chains. A general value of 9 < C(infinity) < 16 and 3 < L(p) < 5 nm can now be estimated with statistical confidence for all galactomannans. According to our statistical analysis, the chain persistence length was found to be insensitive to the degree of galactose substitution.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Picout DR,Ross-Murphy SB,Jumel K,Harding SE

doi

10.1021/bm025517c

keywords:

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

761-7

issue

4

eissn

1525-7797

issn

1526-4602

pii

bm025517c

journal_volume

3

pub_type

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