Cartilage-derived morphogenetic protein-1 and -2 are endogenously expressed in healthy and osteoarthritic human articular chondrocytes and stimulate matrix synthesis.


OBJECTIVE:We investigated whether chondrocytes derived from osteoarthritic cartilage may lose their responsiveness to cartilage-derived morphogenetic protein-1, -2 (CDMP-1, -2) and osteogenic protein-1 (OP-1) compared with healthy cells, thus leading to an impaired maintenance of matrix integrity. DESIGN:Chondrocytes were isolated from articular cartilage from patients with and without osteoarthritic lesions. Cells were grown as monolayer cultures for 7 days in a chemically defined serum-free basal medium (BM) in the presence of recombinant CDMP-1, -2, and OP-1. Glycosaminoglycan synthesis was measured by [35S]Sulfate incorporation into newly synthesized macromolecules. Cell proliferation was investigated by [3H]Thymidine incorporation. The endogenous gene expression of CDMPs/OP-1 and their respective type I and type II receptors was examined using RT-PCR. The presence of CDMP proteins in tissue and cultured cells was detected by Western immunoblots. RESULTS:mRNAs coding for CDMPs and their respective receptors are endogenously expressed not only in healthy, but also in osteoarthritic cartilage. CDMP proteins are present in both normal and osteoarthritic articular cartilage and cultured chondrocytes. CDMP-1, CDMP-2 and OP-1 markedly increased glycosaminoglycan synthesis in both healthy (P< 0.01) and osteoarthritic (P< 0.05) human articular chondrocytes. A comparison of the glycosaminoglycan biosynthetic activity between healthy and osteoarthritic samples revealed no detectable difference, neither in stimulated nor in unstimulated cultures. [(3)H]Thymidine incorporation showed that CDMPs/OP-1 did not affect cell proliferation in vitro. CONCLUSION:CDMPs and OP-1 exert their anabolic effects on both healthy and osteoarthritic chondrocytes indicating no loss in responsiveness to these growth factors in OA. The endogenous expression of CDMPs/OP-1 and their receptors suggest an important role in cartilage homeostasis.


Bobacz K,Gruber R,Soleiman A,Graninger WB,Luyten FP,Erlacher L





Has Abstract


2002-05-01 00:00:00














  • Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System.

    abstract:OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Custers RJ,Creemers LB,Verbout AJ,van Rijen MH,Dhert WJ,Saris DB

    更新日期:2007-11-01 00:00:00

  • The effect of waiting time on health and quality of life outcomes and costs of medication in hip replacement patients: a randomized clinical trial.

    abstract:OBJECTIVE:To evaluate the effect of waiting time on health and quality of life outcomes and costs of medication in total hip replacement (THR) patients in a randomized clinical trial. METHODS:395 THR patients were recruited into the study. When placed on the waiting list, patients were randomized into a short (< or =3...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Tuominen U,Sintonen H,Hirvonen J,Seitsalo S,Paavolainen P,Lehto M,Hietaniemi K,Blom M

    更新日期:2009-09-01 00:00:00

  • Flow cytometric analysis of the human articular chondrocyte phenotype in vitro.

    abstract:OBJECTIVE:To develop flow cytometry for the study of human articular cartilage cell phenotype and to validate the method on chondrocytes cultured in different in-vitro systems. METHODS:Chondrocyte phenotype was modulated by culturing the cells under different in-vitro conditions: i.e. in monolayer and in suspension cu...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Wang L,Verbruggen G,Almqvist KF,Elewaut D,Broddelez C,Veys EM

    更新日期:2001-01-01 00:00:00

  • Prevalence of radiographic hip osteoarthritis is increased in high bone mass.

    abstract:OBJECTIVE:Epidemiological studies have shown an association between increased bone mineral density (BMD) and osteoarthritis (OA), but whether this represents cause or effect remains unclear. In this study, we used a novel approach to investigate this question, determining whether individuals with High Bone Mass (HBM) h...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hardcastle SA,Dieppe P,Gregson CL,Hunter D,Thomas GE,Arden NK,Spector TD,Hart DJ,Laugharne MJ,Clague GA,Edwards MH,Dennison EM,Cooper C,Williams M,Davey Smith G,Tobias JH

    更新日期:2014-08-01 00:00:00

  • Optical clearing in collagen- and proteoglycan-rich osteochondral tissues.

    abstract:OBJECTIVE:Recent developments in optical clearing and microscopy technology have enabled the imaging of intact tissues at the millimeter scale to characterize cells via fluorescence labeling. While these techniques have facilitated the three-dimensional (3D) cellular characterization within brain and heart, study of de...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Neu CP,Novak T,Gilliland KF,Marshall P,Calve S

    更新日期:2015-03-01 00:00:00

  • Foot progression angle and the knee adduction moment: a cross-sectional investigation in knee osteoarthritis.

    abstract:OBJECTIVE:To test the hypothesis that an association exists between the characteristics of the knee adduction moment and foot progression angle (FPA) in asymptomatic individuals and those with mild to moderate and severe knee osteoarthritis (OA). DESIGN:Fifty asymptomatic individuals, 46 patients with mild to moderate...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Rutherford DJ,Hubley-Kozey CL,Deluzio KJ,Stanish WD,Dunbar M

    更新日期:2008-08-01 00:00:00

  • A pilot study of the reproducibility and validity of measuring knee subchondral bone density in the tibia.

    abstract:OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Dore D,Ding C,Jones G

    更新日期:2008-12-01 00:00:00

  • Human genome-wide expression analysis reorients the study of inflammatory mediators and biomechanics in osteoarthritis.

    abstract::A major objective of this article is to examine the research implications of recently available genome-wide expression profiles of cartilage from human osteoarthritis (OA) joints. We propose that, when viewed in the light of extensive earlier work, this novel data provides a unique opportunity to reorient the design o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Sandy JD,Chan DD,Trevino RL,Wimmer MA,Plaas A

    更新日期:2015-11-01 00:00:00

  • Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis.

    abstract:OBJECTIVE:To investigate the role of Vhl in maintaining the integrity of articular cartilage and in the development of experimental osteoarthritis (OA). METHOD:Histology of articular cartilage and subchondral bone in both Vhl cKO and WT mice were analyzed by histopathology and micro-CT. Articular cartilage destruction...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Weng T,Xie Y,Yi L,Huang J,Luo F,Du X,Chen L,Liu C,Chen D,Chen L

    更新日期:2014-08-01 00:00:00

  • REMOVED: Generation of cartilage microtissues by hanging drops: effect of different culture conditions.

    abstract::This article has been removed, consistent with Elsevier Policy on Article Withdrawal ( The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Martinez I

    更新日期:2007-04-27 00:00:00

  • Patellofemoral morphology and alignment: reference values and dose-response patterns for the relation to MRI features of patellofemoral osteoarthritis.

    abstract:OBJECTIVE:We aimed to (1) determine reference values for trochlear morphology and patellofemoral (PF) alignment in adults without magnetic resonance imaging (MRI)-defined PF full thickness cartilage damage or knee pain; and (2) evaluate dose-response patterns for these measures with prevalent MRI-defined PF structural ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Macri EM,Felson DT,Zhang Y,Guermazi A,Roemer FW,Crossley KM,Khan KM,Stefanik JJ

    更新日期:2017-10-01 00:00:00

  • Brace treatment for osteoarthritis of the knee: a prospective randomized multi-centre trial.

    abstract:OBJECTIVE:To evaluate the effect of a brace intended to reduce load in patients with medial or lateral compartmental osteoarthritis (OA) and concurrent varus or valgus alignment, respectively. DESIGN:This multi-centre randomized controlled trial (performed 2001-2003) studies the additive effect of a brace intended to ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Brouwer RW,van Raaij TM,Verhaar JA,Coene LN,Bierma-Zeinstra SM

    更新日期:2006-08-01 00:00:00

  • Evidence for functional ATP-sensitive (K(ATP)) potassium channels in human and equine articular chondrocytes.

    abstract:OBJECTIVE:Chondrocytes are highly sensitive to variations in extracellular glucose and oxygen levels in the extracellular matrix. As such, they must possess a number of mechanisms to detect and respond to alterations in the metabolic state of cartilage. In other organs such as the pancreas, heart and brain, such detect...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mobasheri A,Gent TC,Nash AI,Womack MD,Moskaluk CA,Barrett-Jolley R

    更新日期:2007-01-01 00:00:00

  • Prevalence of cam and pincer-type deformities on hip MRI in an asymptomatic young Swiss female population: a cross-sectional study.

    abstract:OBJECTIVES:Femoroacetabular impingement is proposed to cause early osteoarthritis (OA) in the non-dysplastic hip. We previously reported on the prevalence of femoral deformities in a young asymptomatic male population. The aim of this study was to determine the prevalence of both femoral and acetabular types of impinge...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Leunig M,Jüni P,Werlen S,Limacher A,Nüesch E,Pfirrmann CW,Trelle S,Odermatt A,Hofstetter W,Ganz R,Reichenbach S

    更新日期:2013-04-01 00:00:00

  • T-cell mediated inflammatory pathway in osteoarthritis.

    abstract::Osteoarthritis (OA) is not caused by a simple consequence of aging and cartilage degradation. Based on the conventional paradigm, OA has been considered a degenerative joint disorder. However, the dominant clinical symptom has been characterized by a non-infectious chronic inflammatory condition with infiltration of i...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Nakamura H,Yoshino S,Kato T,Tsuruha J,Nishioka K

    更新日期:1999-07-01 00:00:00

  • Detection of changes in cartilage water content using MRI T2-mapping in vivo.

    abstract:OBJECTIVES:Osteoarthritis (OA) is the most prevalent chronic disease in the elderly, and it is generally diagnosed at an advanced state when treatment is difficult if not impossible. The early form of OA is characterized by an elevated water content in the cartilage tissue. The purpose of this study was to verify in vi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Liess C,Lüsse S,Karger N,Heller M,Glüer CC

    更新日期:2002-12-01 00:00:00

  • Sedentary behavior and blood pressure control among osteoarthritis initiative participants.

    abstract:OBJECTIVE:To examine the association between sedentary behavior and blood pressure (BP) among Osteoarthritis Initiative (OAI) participants. DESIGN:We conducted a cross-sectional analysis of the OAI 48-month visit participants whose physical activity was measured using accelerometers. Participants were classified into ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Sohn MW,Manheim LM,Chang RW,Greenland P,Hochberg MC,Nevitt MC,Semanik PA,Dunlop DD

    更新日期:2014-09-01 00:00:00

  • Management options for femoroacetabular impingement: a systematic review of symptom and structural outcomes.

    abstract:OBJECTIVE:The optimal therapy for femoroacetabular impingement (FAI) is unclear. The aim of this systematic review was to examine the evidence for surgical and non-surgical treatment of FAI on symptom and structural outcomes. DESIGN:MEDLINE and EMBASE were searched electronically. Surgical and non-surgical management ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Fairley J,Wang Y,Teichtahl AJ,Seneviwickrama M,Wluka AE,Brady SRE,Hussain SM,Liew S,Cicuttini FM

    更新日期:2016-10-01 00:00:00

  • C-reactive protein as a biomarker of emergent osteoarthritis.

    abstract:OBJECTIVE:We evaluated C-reactive protein (C-RP), a quantitative marker of the acute phase response, as a potential biomarker of prevalent and incident osteoarthritis of the knee (OAK). METHODS:Serum C-reactive protein concentrations were characterized with ultrasensitive rate nephelometry in a population-based sample...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Sowers M,Jannausch M,Stein E,Jamadar D,Hochberg M,Lachance L

    更新日期:2002-08-01 00:00:00

  • Site-dependent changes in structure and function of lapine articular cartilage 4 weeks after anterior cruciate ligament transection.

    abstract:OBJECTIVE:The aim of this study was to investigate the site-dependent changes in the structure and function of articular cartilage in the lapine knee joint at a very early stage of osteoarthritis (OA), created experimentally by anterior cruciate ligament transection (ACLT). METHODS:Unilateral ACLT was performed in eig...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mäkelä JT,Rezaeian ZS,Mikkonen S,Madden R,Han SK,Jurvelin JS,Herzog W,Korhonen RK

    更新日期:2014-06-01 00:00:00

  • Sustained efficacy of a single intra-articular dose of FX006 in a rat model of repeated localized knee arthritis.

    abstract:OBJECTIVE:To evaluate the efficacy of a single intra-articular (IA) dose of FX006, an extended-release formulation of triamcinolone acetonide (TCA) in poly(lactic-co-glycolic acid) (PLGA) microspheres, on the sequelae of repeated episodes of synovitis. DESIGN:Three flares of localized synovitis in the right knee of ra...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kumar A,Bendele AM,Blanks RC,Bodick N

    更新日期:2015-01-01 00:00:00

  • Subchondral and epiphyseal bone remodeling following surgical transection and noninvasive rupture of the anterior cruciate ligament as models of post-traumatic osteoarthritis.

    abstract:OBJECTIVE:Animal models are frequently used to study post-traumatic osteoarthritis (PTOA). A common anterior cruciate ligament (ACL) injury model is surgical transection, which may introduce confounding factors from surgery. Noninvasive models could model human injury more closely. The purpose of this study was to comp...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Maerz T,Kurdziel M,Newton MD,Altman P,Anderson K,Matthew HW,Baker KC

    更新日期:2016-04-01 00:00:00

  • Serial kinematic analysis of the canine hindlimb joints after deafferentation and anterior cruciate ligament transection.

    abstract:OBJECTIVE AND DESIGN:Transection of the anterior cruciate ligament 2 weeks after ipsilateral hindlimb deafferentation leads to osteoarthritis of the knee joint within 3 weeks. We analyzed the gait of six dogs that underwent this procedure in order to identify kinematic changes that could account for this rapid joint de...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Vilensky JA,O'Connor BL,Brandt KD,Dunn EA,Rogers PI

    更新日期:1997-05-01 00:00:00

  • Dynamic knee loading is related to cartilage defects and tibial plateau bone area in medial knee osteoarthritis.

    abstract:OBJECTIVE:To evaluate the relationship between dynamic mechanical loading, as indicated by external knee adduction moment (KAM) measures during walking, and measures of articular cartilage morphology and subchondral bone size in people with medial knee osteoarthritis (OA). DESIGN:180 individuals with radiographic medi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Creaby MW,Wang Y,Bennell KL,Hinman RS,Metcalf BR,Bowles KA,Cicuttini FM

    更新日期:2010-11-01 00:00:00

  • Cross-sectional and longitudinal associations between radiographic features and measures of pain and physical function in hand osteoarthritis.

    abstract:OBJECTIVE:Previous longitudinal studies have shown no associations between increasing amount of radiographic hand osteoarthritis (OA) and levels of hand pain/disability. In this longitudinal study, we aimed to study whether radiographic hand OA was related to pain/disability in cross-sectional and longitudinal settings...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Haugen IK,Slatkowsky-Christensen B,Bøyesen P,van der Heijde D,Kvien TK

    更新日期:2013-09-01 00:00:00

  • Recommendations for an update of the 2010 European regulatory guideline on clinical investigation of medicinal products used in the treatment of osteoarthritis and reflections about related clinically relevant outcomes: expert consensus statement.

    abstract:OBJECTIVE:The European Society on Clinical and Economic aspects of Osteoporosis and Osteoarthritis (ESCEO) organised a working group to evaluate the need for updating the current European guideline on clinical investigation of drugs used in the treatment of osteoarthritis (OA). DESIGN:Areas of potential attention were...

    journal_title:Osteoarthritis and cartilage

    pub_type: 共识发展会议,杂志文章,评审


    authors: Reginster JY,Reiter-Niesert S,Bruyère O,Berenbaum F,Brandi ML,Branco J,Devogelaer JP,Herrero-Beaumont G,Kanis J,Maggi S,Maheu E,Richette P,Rizzoli R,Cooper C

    更新日期:2015-12-01 00:00:00

  • Surgical suturing of articular cartilage induces osteoarthritis-like changes.

    abstract:INTRODUCTION:In clinical tissue-engineering-based approaches to articular cartilage repair, various types of flap are frequently used to retain an implanted construct within the defect, and they are usually affixed by suturing. We hypothesize that the suturing of articular cartilage is associated with a loss of chondro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hunziker EB,Stähli A

    更新日期:2008-09-01 00:00:00

  • Tribological rehydration of cartilage and its potential role in preserving joint health.

    abstract:OBJECTIVE:During exercise, cartilage recovers interstitial fluid lost during inactivity, which explains how tissue thickness and joint space are maintained over time. This recovery phenomenon is currently explained by a combination of osmotic swelling during intermittent bath exposure and sub-ambient pressurization dur...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Moore AC,Burris DL

    更新日期:2017-01-01 00:00:00

  • Measurement properties of the WOMAC LK 3.1 pain scale.

    abstract:OBJECTIVE:The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) is applied extensively to patients with osteoarthritis of the hip or knee. Previous work has challenged the validity of its physical function scale however an extensive evaluation of its pain scale has not been reported. Our purpose wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Stratford PW,Kennedy DM,Woodhouse LJ,Spadoni GF

    更新日期:2007-03-01 00:00:00

  • Articular cartilage calcification of the hip and knee is highly prevalent, independent of age but associated with histological osteoarthritis: evidence for a systemic disorder.

    abstract:OBJECTIVES:Based on the concept of a systemic predisposition for articular cartilage calcification (CC), the aim of this study was to determine the prevalence and amount of bilateral CC of hip and knee joints in an unselected sample cohort by high-resolution digital contact radiography (DCR) and to analyze the associat...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hawellek T,Hubert J,Hischke S,Krause M,Bertrand J,Pap T,Püschel K,Rüther W,Niemeier A

    更新日期:2016-12-01 00:00:00