Abstract:
:Infrared and UV spectroscopies have been used to study the assembly of a hairpin nucleotide sequence (nucleotides 3-30) of the 5' non-coding region of the hepatitis C virus RNA (5'-GGCGGGGAUUAUCCCCGCUGUGAGGCGG-3') with a RNA 20mer ligand (5'-CCGCCUCACAAAGGUGGGGU-3') in the presence of magnesium ion and spermidine. The resulting complex involves two helical structural domains: the first one is an intermolecular duplex stem at the bottom of the target hairpin and the second one is a parallel triplex generated by the intramolecular hairpin duplex and the ligand. Infrared spectroscopy shows that N-type sugars are exclusively present in the complex. This is the first case of formation of a RNA parallel triplex with purine motif and shows that this type of targeting RNA strands to viral RNA duplexes can be used as an alternative to antisense oligonucleotides or ribozymes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Carmona P,Molina Mdoi
10.1093/nar/30.6.1333keywords:
subject
Has Abstractpub_date
2002-03-15 00:00:00pages
1333-7issue
6eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract::JSNP is a repository of Japanese Single Nucleotide Polymorphism (SNP) data, begun in 2000 and developed through the Prime Minister's Millennium Project. The aim of this undertaking is to identify and collate up to 150 000 SNPs from the Japanese population, located in genes or in adjacent regions that might influence t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.1.158
更新日期:2002-01-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1977-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw644
更新日期:2016-09-06 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr080
更新日期:2011-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp347
更新日期:2009-07-01 00:00:00
abstract::The sequence of the 1173 nt 12S kinetoplast ribosomal RNA from Leishmania tarentolae was determined from the maxicircle DNA sequence, and the 5' and 3' ends localized by primer runoff and S1 nuclease protection experiments. The gene was shown to be free of introns by S1 nuclease analysis. A partial secondary structure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.7.2337
更新日期:1985-04-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1095
更新日期:2018-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki209
更新日期:2005-01-26 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.17.6075
更新日期:1985-09-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2017-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1981-11-11 00:00:00
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更新日期:1998-10-15 00:00:00
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pub_type: 杂志文章
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更新日期:1998-06-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:1992-06-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.13.2585
更新日期:2000-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.22.6595
更新日期:1990-11-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-10-25 00:00:00