Abstract:
:Neurons in culture rely on the supply of exogenous cysteine for their glutathione synthesis. After application of cysteine to neuron-rich primary cultures, the glutathione content was doubled after a 4-hr incubation. The dipeptide cysteinylglycine (CysGly) was able to substitute for cysteine as exogenous glutathione precursor. In kidneys, the ectopeptidase aminopeptidase N (ApN) has been reported to hydrolyze CysGly. Expression of mRNA of ApN in rat brain and cultured rat neurons was demonstrated by reverse transcriptase polymerase chain reaction and sequencing of the cDNA fragment obtained. In addition, the presence of ApN protein in cultured neurons was demonstrated by its immunocytochemical localization. In the presence of an activity-inhibiting antiserum against ApN the utilization of CysGly as neuronal glutathione precursor was completely prevented, whereas that of cysteine plus glycine was not affected. The data presented demonstrates that cultured rat neurons express ApN and that this ectopeptidase participates in the utilization of CysGly as precursor for neuronal glutathione.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Dringen R,Gutterer JM,Gros C,Hirrlinger Jdoi
10.1002/jnr.10042keywords:
subject
Has Abstractpub_date
2001-12-01 00:00:00pages
1003-8issue
5eissn
0360-4012issn
1097-4547pii
10.1002/jnr.10042journal_volume
66pub_type
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