Abstract:
:Although protein kinase Cdk5-p35 is important in many aspects of the development and function of the central nervous system, relatively little is known about its regulation. In the present study, we examined the relationship between the association of this kinase with membranes and its activity in perinatal and adult rat brains. Cdk5-p35 in perinatal brain exhibited higher activity than that found in adult tissue. Gel filtration chromatography revealed that a portion of Cdk5-p35 from fetal brain occurred as a soluble complex, whereas Cdk5-p35 in adult brain occurred predominantly as a membrane-bound complex. Furthermore, soluble Cdk5-p35 in perinatal brain displayed elevated kinase activity, whereas membrane-bound Cdk5-p35 was highly active only in the presence of detergent. This more active soluble form of Cdk5-p35 correlated to a form in which p35 was phosphorylated, whereas the less active membrane-bound form of Cdk5 correlated to the dephosphorylated form of p35, as evidenced by a downward shift in electrophoretic mobility. Cdk5 activity and transition from soluble to membrane-associated compartments could be modulated by conditions that affected the phosphorylation or dephosphorylation of p35. For example, dephosphorylation of p35 in brain extracts was suppressed by selective inhibition of protein phosphatase-1. Together, these results suggest that the kinase activity of Cdk5-p35 is regulated through its association with membranes, which in turn is under the control of Cdk5-dependent phosphorylation and protein phosphatase-1-dependent dephosphorylation of p35.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Sato K,Zhu YS,Saito T,Yotsumoto K,Asada A,Hasegawa M,Hisanaga Sdoi
10.1002/jnr.21438subject
Has Abstractpub_date
2007-11-01 00:00:00pages
3071-8issue
14eissn
0360-4012issn
1097-4547journal_volume
85pub_type
杂志文章abstract::Ionotropic N-methyl-D-aspartate (NMDA) receptor agonists, L-aspartic acid (L-Asp) and NMDA, have been shown to inhibit histamine-stimulated acid secretion, but their effect on gastric mucosal blood flow (GMBF) is largely unknown. The aim of this study was to investigate whether L-Asp and NMDA inhibit histamine-stimula...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20202
更新日期:2004-09-01 00:00:00
abstract::Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20835
更新日期:2006-06-01 00:00:00
abstract::Primary cultures of cerebral glia derived from neonatal rat brain were utilized to determine 1) the developmental changes of dolichol-linked oligosaccharide and N-linked glycoprotein biosyntheses, 2) the enzymatic correlates of these developmental changes, and 3) the temporal relations between the biosyntheses of doli...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200409
更新日期:1988-08-01 00:00:00
abstract::Four brain-specific, DNase I hypersensitive sites (HSS) have been mapped to the 5' flanking region of the mouse 68-kDa neurofilament gene. These sites are contained within a 1.7-kb sequence that confers neuronal specificity of expression of this gene in transgenic mice. To identify DNA sequences that might be involved...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490320204
更新日期:1992-06-01 00:00:00
abstract::Although it has been well established that the drug efflux pump P-glycoprotein (P-gp) protects the brain against the entry of cytotoxic drugs, its real in situ localization, i.e., at brain capillary endothelial cells or on astrocyte foot processes, is still controversial. The aim of this study was to compare the expre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000601)60:5<594::AID-JNR
更新日期:2000-06-01 00:00:00
abstract::Destruction of the ventromedial hypothalamic nuclei (VMN) in the weanling rat without injury to the median eminence results in a series of somatic, endocrine, and metabolic changes that are characterized by normal food and water intake but decreased linear growth, normal body weight but increased carcass fat and reduc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490020202
更新日期:1976-01-01 00:00:00
abstract::Netrin-1 regulates axon extension during embryonic development and is expressed by neurons and myelinating oligodendrocytes in the adult CNS. To investigate the potential role of netrin-1 after spinal cord injury, we examined the expression of netrin-1 and netrin receptors after sagittal myelotomy in adult rats. This ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21070
更新日期:2006-12-01 00:00:00
abstract::FE65 is a key "adapter" protein that links a multiprotein complex to an intracellular domain of beta-amyloid precursor protein (betaPP). Its overexpression modulates the trafficking of betaPP and facilitates the generation of beta-amyloid (Abeta). FE65 is predominantly expressed in brain tissues. An exon 9-inclusive i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000401)60:1<73::AID-JNR8
更新日期:2000-04-01 00:00:00
abstract::In eukaryotes, mitochondria are critical for cellular bioenergetics and mediating apoptosis. The transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) is an important regulator of mitochondrial biogenesis and function. However, the role of PGC-1alpha in neuronal a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22354
更新日期:2010-07-01 00:00:00
abstract::We used a modified pial vessel disruption (PVD) protocol with adult male Wistar rats to mimic small-vessel stroke in the cerebral cortex. Within 3 weeks, this lesion develops into a single lacuna-like cavity, which is fluid-filled and encapsulated by reactive astrocytes. Minocycline treatment that commences 1 hr after...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23223
更新日期:2013-07-01 00:00:00
abstract::Few diseases in clinical medicine cause as much diagnostic consternation as central nervous system (CNS) vasculitis because of its varying modes of presentation and frequently overlapping clinical and pathological features. There are no pathognomonic clinical or laboratory findings. The purpose of the present retrospe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10180
更新日期:2002-03-15 00:00:00
abstract::The tissue- and cell-specific expression of three neutral glycosphingolipids, gangliotetraosylceramide (GA1), gangliopentaosylceramide (GalNAc-GA1), and the novel 3-O-acetyl-sphingosine-series glycolipid (FMC-5), were examined with monospecific polyclonal antibodies. Immunohistochemical studies of rodent brain cross-s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21419
更新日期:2007-10-01 00:00:00
abstract::The cellular localization of calpain is important in understanding the roles that calpain may play in physiological function. We, therefore, examined calpain expression, activity, and immunofluorescent localization in primary cultures of rat oligodendrocytes. The mRNA expression of m-calpain was 64.8% (P = 0.0033) and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10414
更新日期:2002-11-15 00:00:00
abstract::Prothrombin and its active derivative thrombin are key members of the coagulation system. The only site of extra-hepatic thrombin expression is the central nervous system (CNS), where it is involved in brain development, protection, and regeneration. Thrombin affects various degenerative and ischemic CNS diseases like...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24049
更新日期:2017-10-01 00:00:00
abstract::The multifactorial nature of traumatic brain injury (TBI), especially the complex secondary tissue injury involving intertwined networks of molecular pathways that mediate cellular behavior, has confounded attempts to elucidate the pathology underlying the progression of TBI. Here, systems biology strategies are explo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23503
更新日期:2015-02-01 00:00:00
abstract::Finding inhibitors of the first step of the amyloid cascade, A beta (42) generation, is a major goal of Alzheimer's disease drug development. Two target protease activities, beta-and gamma-secretase, were detected more than 10 years ago but progress in this area has been slow because the enzymes were not identified. U...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.10393
更新日期:2002-11-01 00:00:00
abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24605
更新日期:2021-01-01 00:00:00
abstract::Sulfoglucuronosyl paragloboside (SGPG) is a member of the sulfated glucuronic acid-containing glycolipid (SGGL) family found primarily in peripheral nerves. These glycolipids contain the HNK-1 carbohydrate epitope and are recognized by monoclonal IgM from patients with chronic demyelinating neuropathy and paraproteine...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960615)44:6<586::AID-JNR
更新日期:1996-06-15 00:00:00
abstract::Dorsal root ganglia (DRG) neurons connect the spinal cord and uterine cervix, and are activated at parturition with subsequent stimulation of secondary neurons in the spinal dorsal horn and autonomic areas. Neuropeptide neurotransmitters and receptors have been studied in these areas, but amino acid transmitters, e.g....
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21225
更新日期:2007-05-01 00:00:00
abstract::Adenosine signalling has an important role in cochlear protection from oxidative stress. In most tissues, intracellular adenosine kinase (ADK) is the primary route of adenosine metabolism and the key regulator of intracellular and extracellular adenosine levels. The present study provides the first evidence for ADK di...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22421
更新日期:2010-09-01 00:00:00
abstract::This study tested the hypothesis that sensitivity to the Ca(2+) -induced loss of mitochondrial membrane potential (ΔΨ(m)) and the sensitivity of the loss of ΔΨ to mitochondrial permeability transition pore (PTP) inhibitors are different for neurons and astrocytes. Primary cultures of rat cortical neurons and astrocyte...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22709
更新日期:2011-12-01 00:00:00
abstract::Ferritin is the major iron storage protein and accounts for the majority of the iron in the brain. Thus, ferritin is a key component in protecting the brain from iron induced oxidative damage. The high lipid content, high rate of oxidative metabolism, and high iron content combine to make the brain the organ most susc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370405
更新日期:1994-03-01 00:00:00
abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370117
更新日期:1994-01-01 00:00:00
abstract::Substance P (SP) acts as an immunoregulator by binding to specific functional cell surface receptors on a subpopulation of human T-helper lymphocytes. Receptors with similar properties have also been characterized on the human IM-9 B-lymphoblast cell line. Four distinct proteins of molecular weight (MW) 33,000, 58,000...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180127
更新日期:1987-01-01 00:00:00
abstract::To promote neurite elongation, nerve growth cones must adhere to other surfaces. A complex of integral membrane glycoproteins mediates cell binding to the extracellular glycoproteins fibronectin and laminin (Horwitz et al., J Cell Biol 101:2134-2144, 1985). The receptor complex, named integrin, binds to fibronectin by...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210221
更新日期:1988-10-01 00:00:00
abstract::Most cases of early-onset torsion dystonia are caused by deletion of GAG in the coding region of the DYT1 gene encoding torsinA. This autosomal dominant neurologic disorder is characterized by abnormal movements, believed to originate from neuronal dysfunction in the basal ganglia of the human brain. The torsins (tors...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10567
更新日期:2003-04-15 00:00:00
abstract::Neuron expression of the cytokine tumor necrosis factor-alpha (TNF), and the regulation of the levels of TNF by alpha(2)-adrenergic receptor activation were investigated. Adult rat hippocampal neurons and phorbol ester (PMA)-differentiated SH-SY5Y cells were examined. Intracellular levels of TNF mRNA accumulation, as ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10101
更新日期:2002-01-15 00:00:00
abstract::The termination of chemical neurotransmission in the CNS involves the rapid removal of neurotransmitter from synapses by specific transport systems. Such mechanism operates for the three major amino acid neurotransmitters glutamate, gamma-aminobutyric acid (GABA) and glycine. To date, five different high-affinity Na(+...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.1039
更新日期:2001-03-15 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) is an extensively studied protein that is evolutionarily conserved and widely distributed in the brain of vertebrates. It acts via its cognate receptors TrkB and p75NTR and plays a central role in the developmental neurogenesis, neuronal survival, proliferation, differentiation...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24536
更新日期:2020-05-01 00:00:00
abstract::In order to better characterize the presynaptic GABA receptors regulating glutamate release in the cerebellum [Levi and Gallo, 1981], a number of GABA agonists and GABA transport inhibitors were tested for their ability to potentiate the depolarization-induced release of the glutamate analog D-[3H]aspartate from super...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490100204
更新日期:1983-01-01 00:00:00