Abstract：
PURPOSE:In severe ocular surface diseases, pathologic keratinization of the ordinarily nonkeratinized corneal and conjunctival mucosal epithelia results in severe visual loss. The expression in conjunctivalized corneas of various proteins known to play important roles in the physiological keratinization process in human epidermis was examined to better understand the mechanism of keratinization. METHODS:Conjunctiva covering the cornea was examined in 12 eyes with ocular surface disease in the chronic cicatricial phase. These comprised four Stevens-Johnson syndrome, four ocular cicatricial pemphigoid, and four chemical injuries. Normal conjunctivas from four age-matched individuals served as controls. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate transglutaminase 1 gene expression and immunohistochemistry to study the expression of transglutaminase 1 protein along with other keratinization-related proteins (involucrin, loricrin, filaggrin, and cytokeratins 1 and 10) and cytokeratin pairs 4/13 and 3/12. RESULTS:Semiquantitative RT-PCR showed that transglutaminase 1 mRNA expression was upregulated in keratinized conjunctiva compared with normal. Also, in this tissue, immunohistochemistry demonstrated elevated levels of transglutaminase 1, involucrin, filaggrin, and the cytokeratin pair 1/10. Levels of loricrin and cytokeratin pairs 4/13 and 3/12, however, remained the same. CONCLUSIONS:Various keratinization-related proteins, transglutaminase 1 included, are most likely involved in the pathogenesis of cicatrizing ocular surface diseases.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Nakamura T,Nishida K,Dota A,Matsuki M,Yamanishi K,Kinoshita Skeywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
549-56issue
3eissn
0146-0404issn
1552-5783journal_volume
42pub_type
杂志文章abstract：PURPOSE:Identification of causative mutations for retinal blinding disorders is often limited by restricted understanding of gene expression and underlying molecular mechanisms that trigger degenerative processes. This study was conducted to develop a catalog of canine retina-expressed genes that would provide a unique...
journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.05-1463
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abstract：PURPOSE:To examine the potential of a broad-spectrum kinase inhibitor as a means of neutralizing the effects of contraction promoters on ocular cells. METHODS:The inhibitory effects of a broad-spectrum kinase inhibitor were examined and characterized using an in vitro assay of extracellular matrix contraction by choro...
journal_title：Investigative ophthalmology & visual science
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abstract：PURPOSE:The purpose of this study was to examine the possibility that all-trans-retinoic acid (RA) in the eye is a signal related to changes in scleral extracellular matrix in a primate model of postnatal eye growth. METHODS:Juvenile marmosets (Callithrix jacchus) were divided into two experimental groups based on the...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
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abstract：PURPOSE:Autoimmune uveitis is a sight-threatening disease in which autoreactive T cells cross the blood-retinal barrier. Molecular mechanisms contributing to the loss of eye immune privilege in this autoimmune disease are not well understood. In this study, the authors investigated the changes in the matrix metalloprot...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6475
更新日期：2011-04-08 00:00:00
abstract：:Using a freezing point depression method, requiring volumes of only 200 nanoliters, the osmolalities of 324 tear samples collected at consecutive 10-minute intervals throughout 8.5-hour periods from each of six healthy young adults were measured. The population mean was found to be 318 mOsm/kg, with a population media...
journal_title：Investigative ophthalmology & visual science
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更新日期：1983-12-01 00:00:00
abstract：:The corneas of 12 eye donors with maturity-onset diabetes were obtained, and the corneal epithelial basement membranes were studied by transmission electron microscopy. Similar tissue was obtained from 12 nondiabetic eye donors who were matched for age (within 2 years) and race. The mean thickness of the corneal epith...
journal_title：Investigative ophthalmology & visual science
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更新日期：1981-04-01 00:00:00
abstract：PURPOSE:To examine whether Na(+)-K(+)-2Cl(-) cotransport has the potential to contribute to corneal endothelial ion and fluid transport in cultured and fresh bovine corneal endothelial cells. METHODS:Cl- and Na+ sensitive fluorescent dyes were used to measure furosemide-dependent ion fluxes in cultured and fresh endot...
journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：2000-02-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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abstract：PURPOSE:To study in an infant rat model of retinopathy of prematurity, the rod photoreceptors, which are known to have attenuated photoresponses. METHODS:Rhodopsin was extracted from whole retinas, the thickness of the rod outer segment (ROS) layer was measured, large phagosomes were counted, and the ROS ultrastructur...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1984-07-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：PURPOSE:To determine whether fellow eyes with normal visual acuity of patients with unilateral neovascular age-related macular degeneration (AMD) have retinal malfunction. METHODS:Foveal cone electroretinograms (ERGs) were recorded from fellow eyes with visual acuities of 20/25 or better of 73 patients with unilateral...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,评审
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1977-07-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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journal_title：Investigative ophthalmology & visual science
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.12-10293
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.07-0860
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journal_title：Investigative ophthalmology & visual science
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
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journal_title：Investigative ophthalmology & visual science
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doi：
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journal_title：Investigative ophthalmology & visual science
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更新日期：2010-08-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1999-05-01 00:00:00
