当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Expression and cellular distribution of alpha(v)integrins in beta(1)integrin-deficient embryonic stem cell-derived cardiac cells.

Expression and cellular distribution of alpha(v)integrins in beta(1)integrin-deficient embryonic stem cell-derived cardiac cells.

Abstract：

:beta(1)integrin-deficient (beta(1)-/-) ES cells showed increased differentiation of cardiac cells characterized by reduced adhesion and high beating frequency. Whereas in whole embryoid body outgrowths of beta(1)-/- cells maximum levels of alpha(v), beta(3)and beta(5)integrin mRNA were delayed and transiently upregulated, in cardiac clusters isolated from beta(1)-/- cells, only beta(3)integrin mRNA levels were enhanced in comparison to wild-type (wt) cells. To answer the question, whether alpha(v)and beta(3)integrins may compensate, at least partially, the loss of beta(1)integrin function during cardiac differentiation, the distribution of alpha(v)and beta(3)integrins in beta(1)-/- and wt pacemaker-like cardiac cells was analyzed. A different distribution of alpha(v)and beta(3)integrins in beta(1)-/- v wt cardiac cells was found. In wt cardiac cells, beta(1)integrin was localized in specialized subsarcolemmal regions, in particular, at focal contacts and costameres, but alpha(v)integrin was diffusely distributed. In contrast, in beta(1)-/- cardiac cells, alpha(v)integrin was preponderantly localized at cell membranes, focal contacts and costameres. beta(3)integrin displayed a diffuse pattern both in wt and in beta(1)-/- pacemaker-like cells at early differentiation stages, whereas at terminal stages, beta(3)was colocalized with sarcomeres in wt, but not in beta(1)-/- pacemaker-like cells. Quantitative immunofluorescence analysis revealed increased alpha(v)and beta(3)integrin levels in beta(1)-/- pacemaker-like cardiac cells. Our results led us to conclude that altered cellular distribution of alpha(v)integrin and upregulation of beta(3)integrin correlate with growth and survival of beta(1)-/- cardiac pacemaker-like cells at an early developmental state. However, alpha(v)and beta(3)integrins cannot functionally compensate the loss of beta(1)integrin during terminal differentiation of cardiac cells implicating that cardiomyocytes require specific beta(1)integrin functions for cardiac specialization.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Guan K,Czyz J,Fürst DO,Wobus AM

doi

10.1006/jmcc.2000.1326

keywords:

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

521-32

issue

3

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(00)91326-9

journal_volume

33

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Type 2 diabetes, mitochondrial biology and the heart.

    abstract：:Diabetes is recognized as an independent risk factor for cardiovascular morbidity and mortality. This is due, in large part, to premature atherosclerosis, enhanced thrombogenicity and activation of systemic inflammatory programs with resultant vascular dysfunction. More enigmatic mechanisms underpinning diabetes-assoc...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.02.001

    authors： Sack MN

    更新日期：2009-06-01 00:00:00

  • Decrease with senescence in the norepinephrine-induced phosphorylation of myofilament proteins in isolated rat cardiac myocytes.

    abstract：:(1) The effects of norepinephrine on protein phosphorylation in isolated rat cardiac ventricular myocytes were determined by autoradiography on 32P-labelled proteins separated by electrophoresis; (2) In cells from young adult rats (6 months old) there was a marked increase due to norepinephrine (10(-8) to 10(-4) M) in...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(89)90678-0

    authors： Sakai M,Danziger RS,Staddon JM,Lakatta EG,Hansford RG

    更新日期：1989-12-01 00:00:00

  • Transcriptomic analysis reveals atrial KCNE1 down-regulation following lung lobectomy.

    abstract：:Lone atrial fibrillation (AF) is associated with various ion channel gene sequence variants, notably the common S38G loss-of-function polymorphism in the KCNE1 K(+) channel ancillary subunit gene. New-onset postoperative AF (POAF) generally occurs 48-72 h after major surgery, particularly following procedures within t...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.05.010

    authors： Heerdt PM,Kant R,Hu Z,Kanda VA,Christini DJ,Malhotra JK,Abbott GW

    更新日期：2012-09-01 00:00:00

  • Oxygen diffusion distance in thyroxine-induced hypertrophic rabbit myocardium.

    abstract：:We studied the oxygen diffusion distance in the rabbit myocardium in untreated animals and in animals treated with thyroxine (T4) for 3 days and 16 days. The subepicardial and subendocardial regions were studied separately. Sixteen days of T4 treatment results in significant cardiac hypertrophy. After 16 days, the per...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(88)80146-9

    authors： Seiden D,Navidad P,Weiss HR

    更新日期：1988-10-01 00:00:00

  • Remote ischemic postconditioning enhances cell retention in the myocardium after intravenous administration of bone marrow mesenchymal stromal cells.

    abstract：:Efficacy of intravenous administration of mesenchymal stromal cells (MSCs) for myocardial infarction (MI) is limited by low cell retention in the damaged myocardium. Previous studies indicated that remote ischemic conditioning could protect against ischemia-reperfusion-induced injury by release of various cytokines in...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.12.016

    authors： Jiang Q,Song P,Wang E,Li J,Hu S,Zhang H

    更新日期：2013-03-01 00:00:00

  • Induction of cardiac beta-adrenergic receptor kinase 1 in rat heart failure caused by coronary ligation.

    abstract：:Beta-adrenergic receptor kinase 1 (beta ARK1) participates in the desensitization of beta-adrenergic receptors by uncoupling the signal transduction. The present study was designed to examine whether neurohumoral increase is crucial for the activation of beta ARK1 in heart failure. Four weeks after the ligation of rat...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1999.0958

    authors： Ishigai Y,Mori T,Moriyama S,Shibano T

    更新日期：1999-06-01 00:00:00

  • Suppression of myocardial mitochondrial respiratory function in acute failing hearts made by a short-term Ca2+ free, high Ca2+ coronary perfusion.

    abstract：:We made acute cardiac failure in excised cross-circulated canine hearts by a new coronary perfusion protocol consisting of Ca2+ free Tyrode perfusion for the first 10 min, high Ca2+ (16 mmol/l) Tyrode perfusion for the next 5 min, and normal Tyrode perfusion for the last 5 min interrupting blood cross circulation. Aft...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(95)90022-5

    authors： Takaki M,Zhao DD,Zhao LY,Araki J,Mori M,Suga H

    更新日期：1995-09-01 00:00:00

  • FGF-2-induced negative inotropism and cardioprotection are inhibited by chelerythrine: involvement of sarcolemmal calcium-independent protein kinase C.

    abstract：:Fibroblast growth factor-2 (FGF-2), administered to the isolated rat heart by perfusion and under constant pressure, is protective against ischemia-reperfusion (I-R). Here we have investigated whether FGF-2 cardioprotection: (a) is dependent on flow modulation; (b) is linked to effects on contractility; (c) is mediate...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1998.0832

    authors： Padua RR,Merle PL,Doble BW,Yu CH,Zahradka P,Pierce GN,Panagia V,Kardami E

    更新日期：1998-12-01 00:00:00

  • The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension.

    abstract：:Pulmonary arterial hypertension (PAH) is associated with refractory vasoconstriction and impaired NO-mediated vasodilatation of the pulmonary vasculature. Vascular tone is regulated by light chain (LC) phosphorylation of both nonmuscle (NM) and smooth muscle (SM) myosins, which are determined by the activities of MLC ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.10.009

    authors： Konik EA,Han YS,Brozovich FV

    更新日期：2013-12-01 00:00:00

  • Regulation of the human tumor necrosis factor-alpha promoter by angiotensin II and lipopolysaccharide in cardiac fibroblasts: different cis-acting promoter sequences and transcriptional factors.

    abstract：:We recently showed that angiotensin (ANG) II as well as mechanical stretch stimulated production of tumor necrosis factor (TNF) in cardiac fibroblasts. Presently, we examined the molecular mechanisms by which ANGII and lipopolysaccharide (LPS) upregulate TNF-alpha gene expression. In neonatal rat cardiac fibroblasts, ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(03)00210-4

    authors： Sato H,Watanabe A,Tanaka T,Koitabashi N,Arai M,Kurabayashi M,Yokoyama T

    更新日期：2003-10-01 00:00:00

  • Anti-apoptotic function of the E2F transcription factor 4 (E2F4)/p130, a member of retinoblastoma gene family in cardiac myocytes.

    abstract：:The E2F4-p130 transcriptional repressor complex is a cell-cycle inhibitor in mitotic cells. However, the role of E2F4/p130 in differentiated cells is largely unknown. We investigated the role of E2F4/p130 in the regulation of apoptosis in postmitotic cardiomyocytes. Here we demonstrate that E2F4 can inhibit hypoxia-in...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.09.004

    authors： Dingar D,Konecny F,Zou J,Sun X,von Harsdorf R

    更新日期：2012-12-01 00:00:00

  • Clinical symptoms of right ventricular failure in experimental chronic pressure load are associated with progressive diastolic dysfunction.

    abstract：BACKGROUND:Right ventricular failure (RVF) due to pressure load is a major cause of death in congenital heart diseases and pulmonary hypertension. The mechanisms of RVF are unknown. We used an experimental approach based upon clinical signs of RVF to delineate functional and biological processes associated with RVF. M...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.11.024

    authors： Borgdorff MA,Koop AM,Bloks VW,Dickinson MG,Steendijk P,Sillje HH,van Wiechen MP,Berger RM,Bartelds B

    更新日期：2015-02-01 00:00:00

  • Systemic hypertension induces disparate localized left ventricular action potential lengthening and altered sensitivity to verapamil in left ventricular myocardium.

    abstract：:Previously, we demonstrated that acute exposure to superfusate containing verapamil suppresses action potential plateau to a greater degree in cells at the tip of the anterior papillary muscle compared to those at its base in normal cat left ventricle (LV) studied in tissue bath. To determine the effects of chronic pr...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80469-2

    authors： Cameron JS,Miller LS,Kimura S,Kaiser CJ,Campbell DR,Kozlovskis PL,Gaide MS,Myerburg RJ,Bassett AL

    更新日期：1986-02-01 00:00:00

  • Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210.

    abstract：:It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.07.001

    authors： Inoue T,Kobirumaki-Shimozawa F,Kagemoto T,Fujii T,Terui T,Kusakari Y,Hongo K,Morimoto S,Ohtsuki I,Hashimoto K,Fukuda N

    更新日期：2013-10-01 00:00:00

  • Intra-tracheal gene delivery of aerosolized SERCA2a to the lung suppresses ventricular arrhythmias in a model of pulmonary arterial hypertension.

    abstract：BACKGROUND:Pulmonary arterial hypertension (PAH) results in right ventricular (RV) failure, electro-mechanical dysfunction and heightened risk of sudden cardiac death (SCD), although exact mechanisms and predisposing factors remain unclear. Because impaired chronotropic response to exercise is a strong predictor of ear...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.11.017

    authors： Strauss B,Sassi Y,Bueno-Beti C,Ilkan Z,Raad N,Cacheux M,Bisserier M,Turnbull IC,Kohlbrenner E,Hajjar RJ,Hadri L,Akar FG

    更新日期：2019-02-01 00:00:00

  • Histochemical alterations of acute and chronic doxorubicin cardiotoxicity.

    abstract：:Histochemical alterations of acute and chronic doxorubicin (DOX) cardiotoxicity in the mouse were assessed by the localization of succinate dehydrogenase (SDH), coenzyme Q10 (CoQ), cytochrome oxidase (COX), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), reduced glutathione (GSH), and intracellular calcium....

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(83)90330-9

    authors： Aversano RC,Boor PJ

    更新日期：1983-08-01 00:00:00

  • May the fibrosis be with you: Is discoidin domain receptor 2 the receptor we have been looking for?

    abstract：:In a recent issue of Journal of Molecular and Cellular Cardiology, George et al. [1] identified discoidin domain receptor 2 (DDR2) as a positive modulator of collagen production in cardiac fibroblasts stimulated with angiotensin II (Ang II). DDR2 is a tyrosine kinase collagen receptor and is associated with pathologic...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 社论

    doi：10.1016/j.yjmcc.2016.01.006

    authors： DeLeon-Pennell KY

    更新日期：2016-02-01 00:00:00

  • Mutation that dramatically alters rat titin isoform expression and cardiomyocyte passive tension.

    abstract：:Titin is a very large alternatively spliced protein that performs multiple functions in heart and skeletal muscles. A rat strain is described with an autosomal dominant mutation that alters the isoform expression of titin. While wild type animals go through a developmental program where the 3.0 MDa N2B becomes the maj...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.02.272

    authors： Greaser ML,Warren CM,Esbona K,Guo W,Duan Y,Parrish AM,Krzesinski PR,Norman HS,Dunning S,Fitzsimons DP,Moss RL

    更新日期：2008-06-01 00:00:00

  • Gene therapy strategies for cardiac electrical dysfunction.

    abstract：:Cardiac disease is frequently associated with abnormalities in electrical function that can severely impair cardiac performance with potentially fatal consequences. The available therapeutic options have some efficacy but are far from perfect. The curative potential of gene therapy makes it an attractive approach for ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2010.07.022

    authors： Greener I,Donahue JK

    更新日期：2011-05-01 00:00:00

  • De-novo collateral formation following acute myocardial infarction: Dependence on CCR2⁺ bone marrow cells.

    abstract：:Wide variation exists in the extent (number and diameter) of native pre-existing collaterals in tissues of different strains of mice, with supportive indirect evidence recently appearing for humans. This variation is a major determinant of the wide variation in severity of tissue injury in occlusive vascular disease. ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.07.020

    authors： Zhang H,Faber JE

    更新日期：2015-10-01 00:00:00

  • Contribution of NO to ischemia-reperfusion injury in the saline-perfused heart: a study in endothelial NO synthase knockout mice.

    abstract：:The contribution of endogenous NO to ischemia-reperfusion injury was studied in isolated perfused hearts of wild-type (WT) and endothelial NO synthase knockout (eNOS-) mice. The hearts were subjected to a 16-min period of global no-flow ischemia and were subsequently reperfused for 1 h. Cardiac contractile function wa...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1998.0921

    authors： Flögel U,Decking UK,Gödecke A,Schrader J

    更新日期：1999-04-01 00:00:00

  • The SCFFBXO3 ubiquitin E3 ligase regulates inflammation in atherosclerosis.

    abstract：:Inflammation is critical in the pathobiology of atherosclerosis. An essential player in the inflammatory process in atherosclerosis are macrophages that scavenge oxidatively modified low-density lipoproteins (OxLDL) deposited in the subendothelium of systemic arteries that secrete a myriad of pro-inflammatory mediator...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.11.006

    authors： Chandra D,Londino J,Alexander S,Bednash JS,Zhang Y,Friedlander RM,Daskivich G,Carlisle DL,Lariviere WR,Nakassa ACI,Ross M,St Croix C,Nyunoya T,Sciurba F,Chen B,Mallampalli RK

    更新日期：2019-01-01 00:00:00

  • Effect of alpha-tocopherol on high energy phosphate metabolite levels in rat heart by 31P-NMR using a Langendorff perfusion technique.

    abstract：:To examine the action of alpha-tocopherol on high energy phosphate compounds, a 31P-NMR technique was applied to perfused Langendorff rat hearts. Rats were treated with tocopherol acetate (25 mg/kg body wt i.p.) for 7 consecutive days. On the 7th day, the rat hearts were isolated for the Langendorff experiment. After ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1993.1119

    authors： Kotegawa M,Sugiyama M,Shoji T,Haramaki N,Ogura R

    更新日期：1993-09-01 00:00:00

  • Extracellular superoxide dismutase regulates cardiac function and fibrosis.

    abstract：:Extracellular superoxide dismutase (EC-SOD) is an antioxidant that protects the heart from ischemia and the lung from inflammation and fibrosis. The role of cardiac EC-SOD under normal conditions and injury remains unclear. Cardiac toxicity, a common side effect of doxorubicin, involves oxidative stress. We hypothesiz...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.08.010

    authors： Kliment CR,Suliman HB,Tobolewski JM,Reynolds CM,Day BJ,Zhu X,McTiernan CF,McGaffin KR,Piantadosi CA,Oury TD

    更新日期：2009-11-01 00:00:00

  • Effects of regional ischemia on the ryanodine-sensitive Ca2+ release channel of canine cardiac sarcoplasmic reticulum.

    abstract：:In mammalian myocardium, muscle contraction is regulated by the rapid release of Ca2+ ions through ryanodine-sensitive Ca2+ release channels present in the intracellular membrane compartment, sarcoplasmic reticulum (SR). In this study, the effects of regional ischemia on intrinsic SR Ca2+ release channel function were...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93181-i

    authors： Darling EM,Lai FA,Meissner G

    更新日期：1992-10-01 00:00:00

  • Gender modulation of Ca(2+) uptake in cardiac mitochondria.

    abstract：BACKGROUND:Mitochondrial calcium overload is an important factor in defining ischemia/reperfusion injury. Since pre-menopausal women are relatively protected from ischemia and heart disease, we tested the hypothesis that gender differences alter Ca(2+) handling in rat cardiac mitochondria. METHODS:Using cardiac mitoch...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.04.023

    authors： Arieli Y,Gursahani H,Eaton MM,Hernandez LA,Schaefer S

    更新日期：2004-08-01 00:00:00

  • An ultrastructural study of the effects of age on sympathetic innervation and atrial tissue in the rat.

    abstract：:Previous work on the rat heart has demonstrated an age-related reduction in catecholamines and a decline in myocardial cell sensitivity to catecholamines in vitro. We used ultrastructural cytochemical techniques to label noradrenergic vesicles of the sympathetic nerve terminals of the rat heart atrium, and addressed t...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(83)90284-5

    authors： McLean MR,Goldberg PB,Roberts J

    更新日期：1983-02-01 00:00:00

  • The effect of vitamin D3 deficiency on the isolated chick heart: hemodynamic, P-31 NMR and membrane studies.

    abstract：:The purpose of this study was to investigate whether vitamin D3 deficiency affects the cardiac function of chick hearts directly or whether the influence is secondary through the hormone's effect on serum calcium levels. To this end, three experimental groups were studied: (a) the control group of vitamin D3 supplemen...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1993.1010

    authors： Hochhauser E,Kushnir T,Navon G,Rehavi M,Barak J,Edelstein S,Vidne B

    更新日期：1993-01-01 00:00:00

  • Alpha adrenergic receptor mechanism: biochemical events.

    abstract：:Immediate post-receptor events following alpha-adrenoceptor stimulation in cardiac ventricular muscle are still largely unknown. Since membrane redox systems appear to be present in cell plasma membranes and may be involved in trans-sarcolemma electron efflux, the possibility that Ca2+ inflow was controlled by electro...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80462-x

    authors： Clark MG,Rattigan S

    更新日期：1986-11-01 00:00:00

  • Reduction of prostacyclin synthesis as a possible cause of transient flow reduction in a partially constricted canine coronary artery.

    abstract：:Coronary blood flow decreases cyclically in a partially occluded coronary artery of anesthetized dogs. Spontaneous aggregation and deaggregation of platelet plugs in the constricted artery have been indicated to be responsible for this phenomenon. A current hypothesis is that platelet aggregation may be determined by ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(84)80040-1

    authors： Tada M,Esumi K,Yamagishi M,Kuzuya T,Matsuda H,Abe H,Uchida Y,Murao S

    更新日期：1984-12-01 00:00:00