当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Myocardial DNA content, ploidy level and cell number in geriatric hearts: post-mortem examinations of human myocardium in old age.

Myocardial DNA content, ploidy level and cell number in geriatric hearts: post-mortem examinations of human myocardium in old age.

Abstract：

:A reduced adaptation rate of the myocardium must, in many cases, be made responsible for heart insufficiency of old patients. The present investigations are aimed towards the question whether heart in old age is accompanied by a loss of myocardial cells and whether a polyploidisation of heart muscle cells takes place thus indicating an adaptive process. A total of 40 human hearts obtained from autopsy material were studied, among them 30 geriatric hearts. Following preparation of the hearts, the myocardium was weighed and the total amount of DNA was determined biochemically. By means of Feulgen cytophotometry the ploidy levels of the heart muscle nuclei were detected. We have used a special method for estimating the total number of heart muscle cells and connective tissue cells in the individual hearts. It could be shown that the total amount of DNA in the myocardium is correlated with the myocardial weight and not with the age of the individual. Instead, DNA synthesis occurs in the heart muscle cells of all age groups depending on the functional activity of the heart. The degree of polyploidisation is closely correlated with myocardial hypertrophy without any influence of age. In hypertrophic hearts the number of connective tissue cells increases from normally 4 to 5 X 10(9) to 12 X 10(9) (in hearts weighing more than 700 g); in hearts with interstitial fibrosis these values are even higher. In normal hearts 1.8 to 2.1 X 10(9) heart muscle cells can be estimated; in hearts weighing more than 500 g numerical hyperplasia of the heart muscle cells is produced, reaching a maximum number of 4.8 X 10(9) muscle cells in excessively hypertrophied hearts weighing more than 800 g. No correlation between age and myocardial cell number (i.e. heart muscle cells or connective tissue cells) could be observed. This means that no agespecific changes occur in the myocardium with regard to the DNA content and cell number. The structural changes found in the hearts of old individuals are produced by extracardial influences (i.e. arteriosclerosis, hypertension, etc.) leading to myocardial hypertrophy. Polyploidisation and augmentation of heart muscle cells in cardiac hypertrophy or decrease in the number of heart muscle cells in heart atrophy may occur at any time of life and are not age-specific.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Adler CP,Friedburg H

doi

10.1016/s0022-2828(86)80981-6

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

39-53

issue

1

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(86)80981-6

journal_volume

18

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Selective translation of mRNAs in the left ventricular myocardium of the mouse in response to acute pressure overload.

    abstract：:During pressure overload hypertrophy, selective changes in cardiac gene expression occur that regulate growth and modify the structural and functional properties of the myocardium. To determine the role of translational mechanisms, a murine model of transverse aortic constriction was used to screen a set of specified ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.10.011

    authors： Spruill LS,Baicu CF,Zile MR,McDermott PJ

    更新日期：2008-01-01 00:00:00

  • Norepinephrine-induced sustained myocardial adaptation to ischemia is dependent on alpha 1-adrenoceptors and protein synthesis.

    abstract：:The authors have shown that stimulation of cardiac alpha 1-adrenoceptors confers immediate cardioprotection in the isolated rat heart against post-ischemic dysfunction, and have recently demonstrated that in vivo treatment of rats with norepinephrine (NE) induces cardiac heat shock protein 72 and myocardial adaptation...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0194

    authors： Meng X,Cleveland JC Jr,Rowland RT,Mitchell MB,Brown JM,Banerjee A,Harken AH

    更新日期：1996-09-01 00:00:00

  • Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

    abstract：:We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals we...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(95)91659-8

    authors： Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

    更新日期：1995-10-01 00:00:00

  • Bradykinin protects against infarction but does not mediate ischemic preconditioning in the isolated rat heart.

    abstract：:The aim of the study was to test if pre-ischemic treatment with bradykinin can protect against infarction in an isolated rat heart model of regional ischemia and reperfusion, and if any such protection is dependent upon activation of protein kinase C (PKC) or mediated through the nitric oxide (NO) pathway. We also inv...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0226

    authors： Bugge E,Ytrehus K

    更新日期：1996-12-01 00:00:00

  • Kinetic analysis of monocarboxylate uptake into perfused rat hearts.

    abstract：:To test a prediction that trans-sarcolemmal lactate movement is carrier mediated, 14C-labelled lactate or pyruvate and 3H-mannitol were transiently infused (2 min) into aerobically perfused rat hearts that had been depleted of intracellular monocarboxylates by a 30 min pre-perfusion in the absence of substrates. Uptak...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(85)80079-1

    authors： Dennis SC,Kohn MC,Anderson GJ,Garfinkel D

    更新日期：1985-10-01 00:00:00

  • Stretch-induced regulation of angiotensinogen gene expression in cardiac myocytes and fibroblasts: opposing roles of JNK1/2 and p38alpha MAP kinases.

    abstract：:The cardiac renin-angiotensin system (RAS) has been implicated in mediating myocyte hypertrophy, remodeling, and fibroblast proliferation in the hemodynamically overloaded heart. However, the intracellular signaling mechanisms responsible for regulation of angiotensinogen (Ao), a substrate of the RAS system, are large...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.09.121

    authors： Lal H,Verma SK,Golden HB,Foster DM,Smith M,Dostal DE

    更新日期：2008-12-01 00:00:00

  • Uterine-derived progenitor cells are immunoprivileged and effectively improve cardiac regeneration when used for cell therapy.

    abstract：:Cell therapy to prevent cardiac dysfunction after myocardial infarction (MI) is less effective in aged patients because aged cells have decreased regenerative capacity. Allogeneic transplanted stem cells (SCs) from young donors are usually rejected. Maintaining transplanted SC immunoprivilege may dramatically improve ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.04.019

    authors： Ludke A,Wu J,Nazari M,Hatta K,Shao Z,Li SH,Song H,Ni NC,Weisel RD,Li RK

    更新日期：2015-07-01 00:00:00

  • Myosin binding protein C phosphorylation in normal, hypertrophic and failing human heart muscle.

    abstract：:Phosphorylation of myosin binding protein C (MyBP-C) was investigated in intraventricular septum samples taken from patients with hypertrophic cardiomyopathy undergoing surgical septal myectomy. These samples were compared with donor heart muscle, as a well-characterised control tissue, and with end-stage failing hear...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.05.020

    authors： Jacques AM,Copeland O,Messer AE,Gallon CE,King K,McKenna WJ,Tsang VT,Marston SB

    更新日期：2008-08-01 00:00:00

  • Development of the coronary arteries in a murine model of transposition of great arteries.

    abstract：:Transposition of great arteries in humans is associated with a wide spectrum of coronary artery patterns. However, no information is available about how this pattern diversity develops. We have studied the development of the coronary arteries in mouse embryos with a targeted mutation of perlecan, a mutation that leads...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(03)00134-2

    authors： González-Iriarte M,Carmona R,Pérez-Pomares JM,Macías D,Costell M,Muñoz-Chápuli R

    更新日期：2003-07-01 00:00:00

  • Transcriptomic analysis reveals atrial KCNE1 down-regulation following lung lobectomy.

    abstract：:Lone atrial fibrillation (AF) is associated with various ion channel gene sequence variants, notably the common S38G loss-of-function polymorphism in the KCNE1 K(+) channel ancillary subunit gene. New-onset postoperative AF (POAF) generally occurs 48-72 h after major surgery, particularly following procedures within t...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.05.010

    authors： Heerdt PM,Kant R,Hu Z,Kanda VA,Christini DJ,Malhotra JK,Abbott GW

    更新日期：2012-09-01 00:00:00

  • Ischemic preconditioning involves dual cardio-protective axes with p38MAPK as upstream target.

    abstract：:The existing literature indicates a crucial role of p38 MAP (mitogen-activated protein) kinase (p38MAPK) and its downstream target MAPKAP kinase 2 (MK2) in ischemic preconditioning (IPC). Accordingly, deletion of MK2 gene should abolish the cardioprotective ability of IPC. Interestingly, we were able to partially prec...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.yjmcc.2007.02.010

    authors： Nagy N,Shiroto K,Malik G,Huang CK,Gaestel M,Abdellatif M,Tosaki A,Maulik N,Das DK

    更新日期：2007-05-01 00:00:00

  • Brain renin-angiotensin system blockade with orally active aminopeptidase A inhibitor prevents cardiac dysfunction after myocardial infarction in mice.

    abstract：:Brain renin-angiotensin system (RAS) hyperactivity has been implicated in sympathetic hyperactivity and progressive left ventricular (LV) dysfunction after myocardial infarction (MI). Angiotensin III, generated by aminopeptidase A (APA), is one of the main effector peptides of the brain RAS in the control of cardiac f...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.12.008

    authors： Boitard SE,Marc Y,Keck M,Mougenot N,Agbulut O,Balavoine F,Llorens-Cortes C

    更新日期：2019-02-01 00:00:00

  • Moderate calorie restriction improves cardiac remodeling and diastolic dysfunction in the Dahl-SS rat.

    abstract：:Caloric restriction extends longevity and reduces the onset of chronic disease in many animal models. Recently, caloric restriction was shown in humans to be associated with lower blood pressure, decreased systemic inflammation, and improved cardiac diastolic parameters. However, the causation and mechanisms of calori...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.07.012

    authors： Seymour EM,Parikh RV,Singer AA,Bolling SF

    更新日期：2006-10-01 00:00:00

  • Mep1a contributes to Ang II-induced cardiac remodeling by promoting cardiac hypertrophy, fibrosis and inflammation.

    abstract：:Pathological cardiac remodeling, characterized by excessive deposition of extracellular matrix proteins and cardiac hypertrophy, leads to the development of heart failure. Meprin α (Mep1a), a zinc metalloprotease, previously reported to participate in the regulation of inflammatory response and fibrosis, may also cont...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2020.11.015

    authors： Ge W,Hou C,Zhang W,Guo X,Gao P,Song X,Gao R,Liu Y,Guo W,Li B,Zhao H,Wang J

    更新日期：2020-12-08 00:00:00

  • Different onset of nucleolar activation in endocardial endothelial cells and cardiomyocytes following pressure overload in rat heart.

    abstract：:We evaluated the time course of activation of endocardial endothelial cells and cardiomyocytes in adult rats with pressure overload induced by the abdominal aortic constriction. The silver staining technique for nucleolar organizer region was used to mark an increased transcriptional activity of the cells. An increase...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0484

    authors： Horký M,Horský P,Kolár F

    更新日期：1997-09-01 00:00:00

  • Increased myocyte fragility following anoxic injury.

    abstract：:The ability of cells in anoxic-perfused hearts to withstand two types of physical stresses (stretching and swelling), in the absence of reoxygenation was determined. Isolated rat hearts were perfused for 20, 40, or 60 mins with anoxic buffer after which hearts were either reoxygenated for 20 mins or exposed for 15 s t...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80353-x

    authors： Vander Heide RS,Ganote CE

    更新日期：1987-11-01 00:00:00

  • Effects of regional ischemia on the ryanodine-sensitive Ca2+ release channel of canine cardiac sarcoplasmic reticulum.

    abstract：:In mammalian myocardium, muscle contraction is regulated by the rapid release of Ca2+ ions through ryanodine-sensitive Ca2+ release channels present in the intracellular membrane compartment, sarcoplasmic reticulum (SR). In this study, the effects of regional ischemia on intrinsic SR Ca2+ release channel function were...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93181-i

    authors： Darling EM,Lai FA,Meissner G

    更新日期：1992-10-01 00:00:00

  • Extracellular superoxide dismutase regulates cardiac function and fibrosis.

    abstract：:Extracellular superoxide dismutase (EC-SOD) is an antioxidant that protects the heart from ischemia and the lung from inflammation and fibrosis. The role of cardiac EC-SOD under normal conditions and injury remains unclear. Cardiac toxicity, a common side effect of doxorubicin, involves oxidative stress. We hypothesiz...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.08.010

    authors： Kliment CR,Suliman HB,Tobolewski JM,Reynolds CM,Day BJ,Zhu X,McTiernan CF,McGaffin KR,Piantadosi CA,Oury TD

    更新日期：2009-11-01 00:00:00

  • Angiotensin II receptor type 1 mRNA is upregulated in atria of patients with end-stage heart failure.

    abstract：:There is increasing evidence that pathological changes in the myocardium during chronic heart failure (CHF) are partly regulated through the activation of the renin-angiotensin system (RAS), an effect mediated by the angiotensin II type 1 receptor (AT1R). We examined the expression of cardiac AT1R mRNA in normal (atri...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0458

    authors： Kaprielian RR,Dupont E,Hafizi S,Poole-Wilson PA,Khaghani A,Yacoub MH,Severs NJ

    更新日期：1997-08-01 00:00:00

  • I(f) blocking potency of ivabradine is preserved under elevated endotoxin levels in human atrial myocytes.

    abstract：:Lower heart rate is associated with better survival in patients with multiple organ dysfunction syndrome (MODS), a disease mostly caused by sepsis. The benefits of heart rate reduction by ivabradine during MODS are currently being investigated in the MODIfY clinical trial. Ivabradine is a selective inhibitor of the pa...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.02.010

    authors： Scheruebel S,Koyani CN,Hallström S,Lang P,Platzer D,Mächler H,Lohner K,Malle E,Zorn-Pauly K,Pelzmann B

    更新日期：2014-07-01 00:00:00

  • Cardiac overexpression of Epac1 in transgenic mice rescues lipopolysaccharide-induced cardiac dysfunction and inhibits Jak-STAT pathway.

    abstract：:Pro-inflammatory cytokines are released in septic shock and impair cardiac function via the Jak-STAT pathway. It is well known that sympathetic stimulation leads to coupling of the β-adrenergic receptor/Gs/adenylyl cyclase, a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP, thereby stimulating prote...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.05.014

    authors： Jin H,Fujita T,Jin M,Kurotani R,Namekata I,Hamaguchi S,Hidaka Y,Cai W,Suita K,Ohnuki Y,Mototani Y,Shiozawa K,Prajapati R,Liang C,Umemura M,Yokoyama U,Sato M,Tanaka H,Okumura S,Ishikawa Y

    更新日期：2017-07-01 00:00:00

  • Longitudinal correlation of biomarkers of cardiac injury, inflammation, and coagulation to outcome in hospitalized COVID-19 patients.

    abstract：BACKGROUND:Cardiac injury, as measured by troponin elevation, has been reported among hospitalized coronavirus disease 2019 (COVID-19) patients and portends a poor prognosis. However, how the dynamics of troponin elevation interplay with inflammation and coagulation biomarkers over time is unknown. We assessed longitud...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2020.08.008

    authors： Li C,Jiang J,Wang F,Zhou N,Veronese G,Moslehi JJ,Ammirati E,Wang DW

    更新日期：2020-10-01 00:00:00

  • Temperature affects human cardiac sarcoplasmic reticulum energy-mediated calcium transport.

    abstract：:Hypothermic cardioplegic solutions are currently used to preserve cardiac function during transportation. However, it has been shown that end-diastolic compliance decreases in donor hearts during reperfusion. Excessively cold temperatures may affect membrane-bound enzymes (Ca2+ ATPase and Ca2+ uptake) which are necess...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1993.1129

    authors： Labow RS,Hendry PJ,Meek E,Keon WJ

    更新日期：1993-10-01 00:00:00

  • Identification and characterization of a transient outward K+ current in human induced pluripotent stem cell-derived cardiomyocytes.

    abstract：BACKGROUND:The ability to recapitulate mature adult phenotypes is critical to the development of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) as models of disease. The present study examines the characteristics of the transient outward current (Ito) and its contribution to the hiPSC-CM action p...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.03.014

    authors： Cordeiro JM,Nesterenko VV,Sicouri S,Goodrow RJ Jr,Treat JA,Desai M,Wu Y,Doss MX,Antzelevitch C,Di Diego JM

    更新日期：2013-07-01 00:00:00

  • Alterations in the baroreceptor reflex control of heart rate in streptozotocin diabetic rats.

    abstract：:Baroreflex control of heart rate was studied in conscious diabetic rats at 12, 24 and 48 weeks after the induction of diabetes with streptozotocin. Baseline blood pressure (mean arterial blood pressure) of diabetic rats was significantly lower at 12 weeks after the induction of diabetes when compared to age-matched co...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80969-5

    authors： Chang KS,Lund DD

    更新日期：1986-06-01 00:00:00

  • Control of steady-state intracellular pH in intact perfused ferret hearts.

    abstract：:To elucidate the role of bicarbonate-dependent mechanisms and Na+/H+ exchange in maintenance of physiological intracellular pH (pHi) under various steady-state conditions, phosphorus NMR spectra were taken in isovolumically-contracting, perfused ferret hearts. Switching the perfusate from HCO3-/CO2 to HEPES buffer sig...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1099

    authors： Kusuoka H,Marban E,Cingolani HE

    更新日期：1994-07-01 00:00:00

  • Sodium channel current loss of function in induced pluripotent stem cell-derived cardiomyocytes from a Brugada syndrome patient.

    abstract：:Brugada syndrome predisposes to sudden death due to disruption of normal cardiac ion channel function, yet our understanding of the underlying cellular mechanisms is incomplete. Commonly used heterologous expression models lack many characteristics of native cardiomyocytes and, in particular, the individual genetic ba...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.10.002

    authors： Selga E,Sendfeld F,Martinez-Moreno R,Medine CN,Tura-Ceide O,Wilmut SI,Pérez GJ,Scornik FS,Brugada R,Mills NL

    更新日期：2018-01-01 00:00:00

  • The response to ischemia in blood perfused vs. crystalloid perfused isolated rat heart preparations.

    abstract：:With a research hypothesis that the behavior of blood perfused hearts was different from that of crystalloid perfused hearts, we tested the null hypothesis that the functional and metabolic status of blood-perfused (paracorporeal oxygenation) and Krebs-Henseleit (bubble oxygenation) perfused Langendorff isolated rat h...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93172-g

    authors： Walters HL 3rd,Digerness SB,Naftel DC,Waggoner JR 3rd,Blackstone EH,Kirklin JW

    更新日期：1992-10-01 00:00:00

  • The effect of ouabain on hearts of cardiomyopathic hamsters: potentiation by isoproterenol.

    abstract：:The isometric twitch properties of papillary muscles from hearts of 30- to 53-day-old cardiomyopathic hamsters (BIO 14.6) were studied before and after exposure to the cardiac glycoside, ouabain. The diseased tissue was weakly responsive to ouabain (3 to 100 microM), as compared to a more appreciable positive inotropi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80368-1

    authors： Rossner KL,Mascarenhas DA

    更新日期：1987-06-01 00:00:00

  • Constitutive expression of HSP 72 in swine heart.

    abstract：:Stress-induced regulation of the 72 kD heat shock protein (HSP 72), the major stress inducible protein in mammalian cells, is mediated by the activation and binding of a heat shock transcription factor (HSF) to a specific sequence in the 5' region of the promoter termed the heat shock element (HSE). In agreement with ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0043

    authors： Locke M,Tanguay RM,Ianuzzo CD

    更新日期：1996-03-01 00:00:00