Abstract:
:Astrocytic endothelin receptors are involved in the appearance of activated astrocytes upon injury of the brain [Ishikawa N. et al. (1997) Eur. J. Neurosci. 9, 895-901; Koyama Y. et al. (1999) Glia 26, 268-271]. To clarify signal transduction triggered by endothelin receptors, we examined the effects of endothelins on protein tyrosine phosphorylation in cultured rat astrocytes. Endothelin-1 (1 nM) increased tyrosine phosphorylation of focal adhesion kinase and paxillin. The tyrosine phosphorylation was also induced by endothelin-1 (1 nM) and Ala(1,3,11,15)-endothelin-1 (10nM), an endothelin-B receptor agonist. BQ788 (100 nM), an endothelin-B receptor antagonist, inhibited the effects of endothelin-3. Orthovanadate (VO(4)(3-)), a tyrosine phosphatase inhibitor, but not bradykinin (1 microM), angiotensin II (100 nM), A23187 (5 microM) and phorbol 12-myristate 13-acetate (100 nM), increased tyrosine phosphorylation of focal adhesion kinase and paxillin. The tyrosine phosphorylation by endothelin-3 was not prevented by pertussis toxin, Ca(2+) chelation, protein kinase C inhibitors (calphostin C and staurosporine) or wortmannin. Immunocytochemical staining showed that endothelin-3 and VO(4)(3-) induced redistribution of focal adhesion kinase and paxillin to focal adhesions concomitant with stress fiber formation in dibutyryl cyclic-AMP-treated astrocytes. Treatment with endothelin-3 and VO(4)(3-) increased focal adhesion kinase and paxillin associated with astrocytic cytoskeletal fraction. In the presence of cytochalasin B, an actin disrupting agent, endothelin-3 and VO(4)(3-) did not phosphorylate focal adhesion kinase and paxillin. Application of cytochalasin B after treatment with endothelin-3 and VO(4)(3-) stimulated dephosphorylation of focal adhesion kinase and paxillin. These results suggest that the associations of focal adhesion kinase and paxillin with cytoskeletal components are required in the endothelin-induced tyrosine phosphorylation of the astrocytic proteins.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Koyama Y,Yoshioka Y,Hashimoto H,Matsuda T,Baba Adoi
10.1016/s0306-4522(00)00330-4keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
219-27issue
1eissn
0306-4522issn
1873-7544pii
S0306-4522(00)00330-4journal_volume
101pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Antagonists acting at the 5-hydroxytryptamine3 receptor are potent anti-emetic agents in cases of cytotoxic- and radiation-induced vomiting, and binding sites for these compounds have been described in brainstem areas known to be involved in mediation of nausea and vomiting. We have used autoradiography to examine the...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90060-h
更新日期:1990-01-01 00:00:00
abstract::When the neural tracer Fluoro-Gold is used to retrogradely label a population of axotomized neurons, cellular labeling can persist in the axotomized nucleus even when Nissl staining indicates that the injured neurons have degenerated. In order to determine the identity of the labeled cells that remain, this study comb...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90096-7
更新日期:1991-01-01 00:00:00
abstract::GABAA-receptors in the brain display a striking structural heterogeneity, which is based on a multiplicity of diverse subunits. The allocation of GABAA-receptor subtypes to identified neurons is essential for an analysis of the functional significance of receptor heterogeneity. Among GABA-receptive neurons, well-chara...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90582-z
更新日期:1993-06-01 00:00:00
abstract::The effect of the availability of synaptic targets on neuronal survival was tested by explanting the cochleovestibular ganglion from embryonic day 3-1/2 chick embryos and maintaining it in the presence or absence of appropriate synaptic target tissues for 14 days in culture. The targets were the inner ear, peripherall...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90053-3
更新日期:1985-09-01 00:00:00
abstract::When human divers or experimental animals are exposed to high pressure, they develop the high-pressure neurological syndrome which is characterized by electroencephalographic changes, and behavioral disturbances. Recently, neurochemical disorders such as a pressure-induced increase in dopamine release have been demons...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90432-2
更新日期:1992-09-01 00:00:00
abstract::In vertebrates, locomotion is associated with changes in respiratory activity, but the neural mechanisms by which this occurs remain unknown. We began examining this in lampreys using a semi-intact preparation of young adult Petromyzon marinus, in which respiratory and locomotor behaviors can be recorded simultaneousl...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.10.019
更新日期:2007-02-09 00:00:00
abstract::Localization of luteotrophic hormone-releasing hormone (LHRH) was examined by immunocytochemistry in untreated male rats and rats that received an anterior hypothalamic deafferentation. Gonadotrophic function was assessed by examining testicular weight and morphology. All of the antisera used in this study were able t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(82)90012-4
更新日期:1982-01-01 00:00:00
abstract::In the present study the effect of cocaine on thymidine, uridine and leucine incorporation was assessed in primary cortical glial and C6 glioma cells. Cocaine exposure for 24 h inhibited thymidine and uridine incorporation in cortical glial and C6 glioma cells. However, the effect of cocaine on uridine incorporation w...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90079-u
更新日期:1993-11-01 00:00:00
abstract::Converging evidence in schizophrenia suggests prefrontal cortical neuronal deficits that correlate with exaggerated subcortical dopamine (DA) functions: Excitotoxic lesion of the ventral hippocampus (VH) in neonatal rats is widely considered a putative animal model of schizophrenia as they lead to characteristic post-...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2003.08.016
更新日期:2003-01-01 00:00:00
abstract::The disturbance of the insulin-signaling pathway plays an important role in Alzheimer's disease. Resistance to insulin signaling renders neurons energy-deficient and vulnerable to oxidization or other metabolic insults and impairs synaptic plasticity. In search of neuroprotective drugs, we synthesized a peptide analog...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.10.069
更新日期:2011-01-26 00:00:00
abstract::Amphetamine induces a pronounced rotation directed ipsilateral to the lesion and lasting about 2 h in rats bearing a unilateral lesion of the nigrostriatal dopaminergic pathway. Implantation of embryonic dopaminergic neurons into the lesioned striatum leads to a compensation of this rotation. However, graft-bearing an...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90491-w
更新日期:1993-04-01 00:00:00
abstract::Both GABA and glycine (Gly) containing neurons send inhibitory projections to the inferior colliculus (IC), whereas inhibitory neurons within the IC are primarily GABAergic. To date, however, a quantitative description of the topographic distribution of GABAergic neurons in the rat's IC and their GABAergic or glyciner...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.12.030
更新日期:2005-01-01 00:00:00
abstract::Professional Pingju actors have been shown to exhibit practice-induced plastic changes in spontaneous regional brain activity; however, whether these changes are present in resting-state regional cerebral blood flow (CBF) remains largely unclear. Here, twenty professional Pingju opera actors and 20 age-, sex-, and han...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.04.007
更新日期:2020-06-01 00:00:00
abstract::The pharmacological features of putative nicotinic acetylcholine receptor sites involved in the release of [3H]noradrenaline were assessed in rat hippocampus. The effect of nicotinic agonists to induce [3H]noradrenaline release was examined in superfused slices. The nicotinic agonists (-)-epibatidine, (+)-anatoxin-a, ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00425-3
更新日期:1997-03-01 00:00:00
abstract::Chronic administration of mu-opioid receptor agonists is known to produce adaptive changes within noradrenergic neurons of the locus coeruleus. Although mu-opioid receptors are densely expressed by locus coeruleus neurons, the effects of acute and chronic administration of agonists on the subcellular distribution of m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00426-2
更新日期:2001-01-01 00:00:00
abstract::Exposure to severe stress leads to development of neuropsychiatric disorders, including depression and Post-Traumatic Stress Disorder (PTSD) in at-risk individuals. Neuropeptide Y (NPY) is associated with resilience or improved recovery. Therefore exogenous administration to the brain has therapeutic potential althoug...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.01.040
更新日期:2013-04-16 00:00:00
abstract::Numerous studies have shown that human endogenous retrovirus W family (HERV-W) envelope gene (env) is related to various diseases but the underlying mechanism has remained poorly understood. Our previous study showed that there was abnormal expression of HERV-W env in sera of patients with schizophrenia. In this paper...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.05.033
更新日期:2013-09-05 00:00:00
abstract::One of the most replicated findings has been that hippocampus volume is decreased in patients with major depressive disorder (MDD). Recent volumetric magnetic resonance imaging (MRI) studies suggest that localized differences in hippocampal volume may be more prominent than global differences. Preclinical and post-mor...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2015.04.047
更新日期:2015-11-19 00:00:00
abstract::Adult neurogenesis occurs in the subgranular zone (SGZ) of the dentate gyrus, where primary neuronal progenitors that express glial fibrillary acidic protein (GFAP) develop into granule neurons. Here, we used transgenic mice with mouse GFAP promoter-controlled enhanced green fluorescent protein (mGFAP-EGFP Tg mice) to...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.12.026
更新日期:2010-03-10 00:00:00
abstract::The schizophrenia-susceptibility gene, dystrobrevin-binding protein 1 (DTNBP1), encodes the dysbindin protein and mediates neurotransmission and neurodevelopment in normal subjects. Functional studies show that DTNBP1 loss may cause deficient presynaptic vesicle transmission, which is related to multiple psychiatric d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.10.020
更新日期:2021-01-01 00:00:00
abstract::The blockade of serotoninergic receptors with methysergide or the activation of alpha(2)-adrenoceptors with moxonidine into the lateral parabrachial nucleus (LPBN) increases water and 0.3 M NaCl intake in rats treated with furosemide (FURO) combined with captopril (CAP). In the present study we investigated the effect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.08.048
更新日期:2009-12-01 00:00:00
abstract::Nickel (Ni(2+)) is a toxic metal that affects the function of several ionic channels. In the N-methyl-d-aspartate (NMDA) subtype of glutamate receptor (NR), it causes activity enhancement of the channels containing the NR2B subunit and voltage-independent inhibition of those containing NR2A. Thus, it may represent a f...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.12.018
更新日期:2011-03-17 00:00:00
abstract::Physiological (pH 7.2 and 7.0) and pathological (pH 6.8 and 6.6) changes of external pH, as they are measured in vivo, were imposed on mouse astrocytes in primary cultures and the effect on components of K+ transport pathways across the cell membrane was measured. Physiological pH changes had no effect at all. Patholo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90024-8
更新日期:1987-01-01 00:00:00
abstract::Light and electron microscopic immunocytochemical methods were used to study the distribution and the morphology of substance P-positive fibers and axon terminals in the dorsal column nuclei of rats and cats, and to determine whether they are part of an ascending input to these nuclei. In rats, substance P-positive fi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90168-4
更新日期:1990-01-01 00:00:00
abstract::Hyperpolarization-activated, cyclic nucleotide-modulated (HCN) channels contribute to rhythmic spontaneous activity in the heart and CNS. Ectopic spontaneous neuronal activity has been implicated in the development and maintenance of acute and chronic hyperalgesia, allodynia and spontaneous pain. Previously, we docume...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.10.048
更新日期:2007-02-23 00:00:00
abstract::Microglia, the resident tissue macrophages of the central nervous system, have a highly differentiated morphology and do not express many of the antigens typically associated with other tissue macrophages. Activation of microglia is associated with a change in morphology and an increase in their repertoire of antigen ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90353-h
更新日期:1993-10-01 00:00:00
abstract::The distributions of the alpha 1-adrenoceptor and its subtypes (alpha 1A and alpha 1B) in human and rat hippocampus are analysed by quantitative receptor autoradiography. alpha 1-Adrenoceptors are labelled by [3H]prazosin. The alpha 1A subtype is visualized by [3H]prazosin after irreversible blockade of alpha 1B adren...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90122-5
更新日期:1991-01-01 00:00:00
abstract::Cocaine- and amphetamine-regulated transcript (CART) and nesfatin-1/nucleobindin 2 (NUCB2) are assumed to play a role in feeding and adaptation to stress. Both peptides are highly expressed in the midbrain non-preganglionic Edinger-Westphal nucleus (npEW), a center implicated in the regulation of stress adaptation and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.07.001
更新日期:2010-10-13 00:00:00
abstract::In urethane-anesthetized rats with body temperature maintained at 39-40 degrees C, electrical stimulation of raphe magnus/pallidus/parapyramidal region within 0.5 mm of the ventral medullary surface reduced arterial blood flow to the tail cutaneous bed (measured with a chronically implanted Doppler ultrasonic flowmete...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00251-2
更新日期:2001-01-01 00:00:00
abstract::A variety of N-methyl-D-aspartate and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonists, and the antiepileptic drug lamotrigine, were examined for their ability to restore locomotion and other behaviours when injected stereotaxically via indwelling cannulae into the striatum or substantia n...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00407-1
更新日期:1997-01-01 00:00:00