[Evaluation of some neutrophil adhesion molecules in pregnancy complicated by diabetes mellitus type 1].

Abstract:

UNLABELLED:The effective functions of human peripheral blood neutrophil are modulated by regulatory molecules depending on receptor agonists and adhesion molecules. OBJECTIVES:Evaluation of percentage of neutrophil adhesion molecules in pregnancy of diabetic mothers as well as their expression on peripheral blood neutrophils. MATERIAL:A-12 pregnant diabetic women at 7-13 and 34-36 weeks of first gestation, B-11 healthy pregnant women match for gestation, age and parity; C-11 healthy non-diabetic women; D-14 diabetic, non-pregnant women. METHODS:MFI expression and percentage of CD18, CD11b, CD62L and CD54 were evaluated resting and activated by 30 min., fMLP preincubation using monoclonal antibodies anti-CD11b and anti-CD62L, employing Flow Cytometer FACSCalibur with computer program CELLquest. RESULTS:Expression of CD11b and CD18 receptor on leukocyte of A, B, D groups was higher spontaneous and after stimulation by fMLP in I-st trimester compare to group C and then lowered in IIIrd. The significant difference in CD62L percentage was noticed resting and activated neutrophils in group A, which was lower compared to C and expression of CD62L on peripheral blood neutrophils was higher in group B compared to other studied groups in Ist trimester. There was an increase in expression of CD54 on resting neutrophils of group D when compared with C. A significant difference between Ist and IIIrd trimester was noticed in group A in expression of CD62L,CD62L/fMLP,CD54 on peripheral blood neutrophils, which were higher in the third trimester. Expression of CD62L was higher in the group A when compared to B. CONCLUSIONS:Diabetes type 1 and pregnancy changed the percentage and MFI of neutrophil adhesion molecules.

journal_name

Ginekol Pol

journal_title

Ginekologia polska

authors

Pertyńska MP,Banasik M,Tchórzewski H,Cypryk K,Wilczyński J

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

480-90

issue

6

eissn

0017-0011

issn

2543-6767

journal_volume

71

pub_type

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